Sperm-induced Ca(2+) oscillations in mouse oocytes and eggs can be mimicked by photolysis of caged inositol 1,4,5-trisphosphate: evidence to support a continuous low level production of inositol 1, 4,5-trisphosphate during mammalian fertilization.


:During mouse fertilization the spermatozoon induces a series of low-frequency long-lasting Ca(2+) oscillations. It is generally accepted that these oscillations are due to Ca(2+) release through the inositol 1,4,5-trisphosphate (InsP(3)) receptor. However, InsP(3) microinjection does not mimic sperm-induced Ca(2+) oscillations, leading to the suggestion that the spermatozoon causes Ca(2+) release by sensitizing the InsP(3) receptor to basal levels of InsP(3). This contradicts recent evidence that the spermatozoon triggers Ca(2+) oscillations by introducing a phospholipase C or else an activator of phospholipase C. Here we show for the first time that sperm-induced Ca(2+) oscillations may be mimicked by the photolysis of caged InsP(3) in both mouse metaphase II eggs and germinal vesicle stage oocytes. Eggs, and also oocytes that had displayed spontaneous Ca(2+) oscillations, gave long-lasting Ca(2+) oscillations when fertilized or when caged InsP(3) was photolyzed. In contrast, oocytes that had shown no spontaneous Ca(2+) oscillations did not generate many oscillations when fertilized or following photolysis of caged InsP(3). Fertilization in eggs was most closely mimicked when InsP(3) was uncaged at relatively low amounts for extended periods. Here we observed an initial Ca(2+) transient with superimposed spikes, followed by a series of single transients with a low frequency; all characteristics of the Ca(2+) changes at fertilization. We therefore show that InsP(3) can mimic the distinctive pattern of Ca(2+) release in mammalian eggs at fertilization. It is proposed that a sperm Ca(2+)-releasing factor operates by generating a continuous small amount of InsP(3) over an extended period of time, consistent with the evidence for the involvement of a phospholipase C.


Dev Biol


Developmental biology


Jones KT,Nixon VL




Has Abstract


2000-09-01 00:00:00














  • PAT-12, a potential anti-nematode target, is a new spectraplakin partner essential for Caenorhabditis elegans hemidesmosome integrity and embryonic morphogenesis.

    abstract::Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Hetherington S,Gally C,Fritz JA,Polanowska J,Reboul J,Schwab Y,Zahreddine H,Behm C,Labouesse M

    更新日期:2011-02-15 00:00:00

  • Cell division in the ciliary ganglion of quail embryos in situ and after back-transplantation into the neural crest migration pathways of chick embryos.

    abstract::Embryonic 4- to 15-day-old quail ciliary ganglia (CG) were grafted into the neural crest migration pathway of 2-day-old chick embryos at the adrenomedullary level of the neural axis. This back-transplantation results in dispersion of cells of the implanted ganglion, their migration in the host embryo, and subsequent p...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Dupin E

    更新日期:1984-10-01 00:00:00

  • The switch from cAMP-independent to cAMP-dependent arrest of meiotic prophase is associated with coordinated GPR3 and CDK1 expression in mouse oocytes.

    abstract::Mammalian oocytes are arrested in meiotic prophase from around the time of birth until just before ovulation. Following an extended period of growth, they are stimulated to mature to the metaphase II stage by a preovulatory luteinizing hormone (LH) surge that occurs with each reproductive cycle. Small, growing oocytes...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Firmani LD,Uliasz TF,Mehlmann LM

    更新日期:2018-02-01 00:00:00

  • Ribbon modulates apical membrane during tube elongation through Crumbs and Moesin.

    abstract::Although the formation and maintenance of epithelial tubes are essential for the viability of multicellular organisms, our understanding of the molecular and cellular events coordinating tubulogenesis is relatively limited. Here, we focus on the activities of Ribbon, a novel BTB-domain containing nuclear protein, in t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kerman BE,Cheshire AM,Myat MM,Andrew DJ

    更新日期:2008-08-01 00:00:00

  • Wnt signaling during development of the gastrointestinal tract.

    abstract::Wnt signaling pathways have been demonstrated to play important roles in controlling tissue patterning and cell proliferation. In the gastrointestinal tract, mutations that lead to activation of the canonical Wnt pathway through beta-catenin result in familial and sporadic colon cancers. The downstream transcription f...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Theodosiou NA,Tabin CJ

    更新日期:2003-07-15 00:00:00

  • Cell degeneration in the developing optic lobes of the sine oculis and small-optic-lobes mutants of Drosophila melanogaster.

    abstract::In the small-optic-lobes (sol) and sine oculis (so) mutants of Drosophila melanogaster extensive cell death occurs in the optic lobes during the first half of pupal development. Gynandromorph flies show that the sol mutation acts primarily on cells of the medulla cortex. Degeneration of medullar ganglion cells occurs ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Fischbach KF,Technau G

    更新日期:1984-07-01 00:00:00

  • Coordinate and noncoordinate regulation of synaptic vesicle protein genes during embryonic development.

    abstract::Because the formation of a mature nerve terminal requires the accumulation of large quantities of synaptic vesicles, the expression of synaptic vesicle proteins would be expected to correlate with synaptogenesis. However, previous studies have provided conflicting evidence on this point. We have examined the developme...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lou X,Bixby JL

    更新日期:1993-09-01 00:00:00

  • Different molecular mechanisms for cAMP regulation of gene expression during Dictyostelium development.

    abstract::Alterations in cAMP concentrations have been implicated in developmentally regulated gene expression in Dictyostelium. Using a variety of culture conditions to control the metabolism of cAMP during cytodifferentiation, I have examined the role of the cyclic nucleotide in development. Conditions which allow intracellul...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kimmel AR

    更新日期:1987-07-01 00:00:00

  • Cyclic AMP induction of Dictyostelium prespore gene expression requires autophagy.

    abstract::Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yamada Y,Schaap P

    更新日期:2019-08-15 00:00:00

  • Reporter gene expression in G2 of the 1-cell mouse embryo.

    abstract::Similar amounts of expression of a luciferase reporter gene that is driven by the SV40 early promoter are detected in G2 of 1-cell mouse embryo following injection of the male pronucleus in either early or mid/late S. No luciferase activity is observed when the male pronucleus is injected in early S and the embryos an...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Ram PT,Schultz RM

    更新日期:1993-04-01 00:00:00

  • Positive and negative regulation of epicardial-mesenchymal transformation during avian heart development.

    abstract::In the developing heart, the epicardium is essential for coronary vasculogenesis as it provides precursor cells that become coronary vascular smooth muscle and perivascular fibroblasts. These precursor cells are derived from the epicardium via epithelial-mesenchymal transformation (EMT). The factors that regulate epic...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Morabito CJ,Dettman RW,Kattan J,Collier JM,Bristow J

    更新日期:2001-06-01 00:00:00

  • Myf5 and MyoD activation define independent myogenic compartments during embryonic development.

    abstract::Gene targeting has indicated that Myf5 and MyoD are required for myogenic determination because skeletal myoblasts and myofibers are missing in mouse embryos lacking both Myf5 and MyoD. To investigate the fate of Myf5:MyoD-deficient myogenic precursor cells during embryogenesis, we examined the sites of epaxial, hypax...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kablar B,Krastel K,Tajbakhsh S,Rudnicki MA

    更新日期:2003-06-15 00:00:00

  • Targeted disruption of the cardiac troponin T gene causes sarcomere disassembly and defects in heartbeat within the early mouse embryo.

    abstract::Cardiac troponin T (cTnT) is a component of the troponin (Tn) complex in cardiac myocytes, and plays a regulatory role in cardiac muscle contraction by anchoring two other Tn components, troponin I (TnI) and troponin C, to tropomyosin (Tm) on the thin filaments. In order to determine the in vivo function of cTnT, we c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Nishii K,Morimoto S,Minakami R,Miyano Y,Hashizume K,Ohta M,Zhan DY,Lu QW,Shibata Y

    更新日期:2008-10-01 00:00:00

  • Biochemical evidence for cell fusion in placentas of mouse aggregation chimeras.

    abstract::Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: West JD,Flockhart JH,Keighren M

    更新日期:1995-03-01 00:00:00

  • Primitive-streak origin of the cardiovascular system in avian embryos.

    abstract::The origin of the avian cardiovascular system from the primitive streak has been mapped by the construction of quail/chick transplantation chimeras, the use of QH-1 (an antiquail endothelial/endocardial cell marker), and injections of a vital fluorescent dye (DiI) into the primitive streak. Our studies reveal that the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Garcia-Martinez V,Schoenwolf GC

    更新日期:1993-10-01 00:00:00

  • The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity.

    abstract::Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Heller RS,Stoffers DA,Liu A,Schedl A,Crenshaw EB 3rd,Madsen OD,Serup P

    更新日期:2004-04-01 00:00:00

  • The TATA binding protein in the sea urchin embryo is maternally derived.

    abstract::The cDNA encoding the TATA binding protein was isolated from 8- to 16-cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C-terminal domains, suggesting a...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Edelmann L,Zheng L,Wang ZF,Marzluff W,Wessel GM,Childs G

    更新日期:1998-12-01 00:00:00

  • Changes in intercellular junctions. II. Modulation in embryonic chick liver in vitro by cytosine arabinoside and dexamethasone.

    abstract::We have used an in vitro model to investigate the relationship between cell proliferation, cell maturation, and intercellular junctional changes that are seen during normal liver development. Chick liver fragments of embryonic stages 21, 28, and 39 were grown in organ culture in Medium 199 with 10% bovine serum albumi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Meyer RA,Overton J

    更新日期:1983-09-01 00:00:00

  • Morphological differentiation of embryonic rat sympathetic neurons in tissue culture. I. Conditions under which neurons form axons but not dendrites.

    abstract::We have examined the morphology of fetal rat sympathetic neurons grown in serum-free medium in the absence of nonneuronal cells. Because cell density can affect phenotypic expression in vitro, the morphological analysis was subdivided into the study of isolated neurons (neurons whose somata were at least 150 micron fr...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bruckenstein DA,Higgins D

    更新日期:1988-08-01 00:00:00

  • The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.

    abstract::Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus (Sp), and each nanos mRNA accumulates specifically in the small micromere (sMic) lineage. We found t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oulhen N,Yoshida T,Yajima M,Song JL,Sakuma T,Sakamoto N,Yamamoto T,Wessel GM

    更新日期:2013-05-01 00:00:00

  • Analysis of corneal development with monoclonal antibodies. I. Differentiation in isolated corneas.

    abstract::Monoclonal antibodies highly selective for developmentally regulated antigens present in the cornea (Zak and Linsenmayer, Dev. Biol. 99, 373-381, 1983) have been used to immunohistochemically evaluate differentiation in intact chick corneas cultured on the chorioallantoic membrane (CAM) of host embryos. One antibody i...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zak NB,Linsenmayer TF

    更新日期:1985-04-01 00:00:00

  • Induction of an epithelial-mesenchymal transition by an in vivo adheron-like complex.

    abstract::The embryonic vertebrate heart consists of two epithelia: the myocardium and endothelium, separated by the myocardial basement membrane (MBM). The myocardium has been shown to induce endothelial transformation into prevalvular mesenchyme in a temporally and site restricted manner. Previously, we hypothesized that the ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mjaatvedt CH,Markwald RR

    更新日期:1989-11-01 00:00:00

  • A divergent Tbx6-related gene and Tbx6 are both required for neural crest and intermediate mesoderm development in Xenopus.

    abstract::T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Callery EM,Thomsen GH,Smith JC

    更新日期:2010-04-01 00:00:00

  • The "beginnings" of the neural crest.

    abstract:FOREWORD:The neural crest has been the main object of my investigations during my career in science, up to now. It is a fascinating topic for an embryologist because of its two unique characteristics: its large degree of multipotency and the fact that its development involves a phase during which its component cells mi...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Le Douarin NM,Dupin E

    更新日期:2018-12-01 00:00:00

  • Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis.

    abstract::Metazoan development involves a myriad of dynamic cellular processes that require cytoskeletal function. Nonmuscle myosin II plays essential roles in embryonic development; however, knowledge of its role in post-embryonic development, even in model organisms such as Drosophila melanogaster, is only recently being reve...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Franke JD,Montague RA,Kiehart DP

    更新日期:2010-09-15 00:00:00

  • The regulatory genome and the computer.

    abstract::The definitive feature of the many thousand cis-regulatory control modules in an animal genome is their information processing capability. These modules are "wired" together in large networks that control major processes such as development; they constitute "genomic computers." Each control module receives multiple in...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Istrail S,De-Leon SB,Davidson EH

    更新日期:2007-10-15 00:00:00

  • Distinct enhancers at the Pax3 locus can function redundantly to regulate neural tube and neural crest expressions.

    abstract::Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory neural crest during embryonic development. We have previously identified cis-acting enhancer elements within the proximal upstream genomic region of Pax3 that are sufficient to direct functional expression of Pax3 in neu...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Degenhardt KR,Milewski RC,Padmanabhan A,Miller M,Singh MK,Lang D,Engleka KA,Wu M,Li J,Zhou D,Antonucci N,Li L,Epstein JA

    更新日期:2010-03-15 00:00:00

  • Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis.

    abstract::The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Johnson JL,Lu C,Raharjo E,McNally K,McNally FJ,Mains PE

    更新日期:2009-06-15 00:00:00

  • Effect of an inhibitory mutant of the FGF receptor on mesoderm-derived alpha-smooth muscle actin-expressing cells in Xenopus embryo.

    abstract::Epithelial-mesenchymal interactions are of major importance during development to direct correct differentiation and morphogenesis of embryonic tissues. One subset of lateral mesoderm-derived mesenchymal cells will form the smooth muscle (SM) layer of the primary epithelial lining of hollow internal organs. It has bee...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Saint-Jeannet JP,Thiery JP,Koteliansky VE

    更新日期:1994-08-01 00:00:00

  • Multiple Shh signaling centers participate in fungiform papilla and taste bud formation and maintenance.

    abstract::The adult fungiform taste papilla is a complex of specialized cell types residing in the stratified squamous tongue epithelium. This unique sensory organ includes taste buds, papilla epithelium and lateral walls that extend into underlying connective tissue to surround a core of lamina propria cells. Fungiform papilla...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Liu HX,Ermilov A,Grachtchouk M,Li L,Gumucio DL,Dlugosz AA,Mistretta CM

    更新日期:2013-10-01 00:00:00