Expression of the Distalless-B gene in Ciona is regulated by a pan-ectodermal enhancer module.


:The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, which are also arranged in convergently transcribed bigene clusters. Sequence analysis of the C. intestinalis Dll-A-B cluster reveals a 378bp region upstream of Ci-Dll-B, termed B1, which is highly conserved with the corresponding region from the congener Ciona savignyi. The B1 element is necessary and sufficient to drive expression of a lacZ reporter gene in a pattern mimicking the endogenous expression of Ci-Dll-B at gastrula stages. This expression pattern which is specific to the entire animal hemisphere is activated preferentially in posterior, or b-lineage, cells by a central portion of B1. Expression in anterior, or a-lineage cells, can be activated by this central portion in combination with the distal part of B1. Anterior expression can also be activated by the central part of B1 plus both the proximal part of B1 and non-conserved sequence upstream of B1. Thus, cis-regulation of early Ci-Dll-B expression is activated by a required submodule in the center of B1, driving posterior expression, which works in combination with redundant submodules that respond to differentially localized anterior factors to produce the total animal hemisphere expression pattern. Interestingly, the intergenic region of the cluster, which is important for expression of the Dlx genes in vertebrates, does not have a specific activating function in the reporter genes tested, but acts as an attenuator in combination with upstream sequences.


Dev Biol


Developmental biology


Irvine SQ,Vierra DA,Millette BJ,Blanchette MD,Holbert RE




Has Abstract


2011-05-15 00:00:00














  • The responses of pollen to applied electrical fields.

    abstract::The growing pollen tubes of tomato and tobacco were exposed to electrical fields and the effect on the direction of growth was measured. They responded by turning toward the positive electrode (anode) and gave a detectable response in fields as small as 0.1 mV/tube diameter. The sites of germination of tobacco pollen ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wang C,Rathore KS,Robinson KR

    更新日期:1989-12-01 00:00:00

  • Ventral closure, headfold fusion and definitive endoderm migration defects in mouse embryos lacking the fibronectin leucine-rich transmembrane protein FLRT3.

    abstract::The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. XFLRT3, a Nodal/TGFbeta target, regulates cell adhesion and modulates FGF signalling during Xenopus gast...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Maretto S,Müller PS,Aricescu AR,Cho KW,Bikoff EK,Robertson EJ

    更新日期:2008-06-01 00:00:00

  • Left and right ventricular contributions to the formation of the interventricular septum in the mouse heart.

    abstract::Mammalian heart development involves complex morphogenetic events which lead to the formation of fully separated left and right atrial and ventricular chambers from a tubular heart. Separation of left and right ventricular chambers is dependent on a single structure, the interventricular septum (IVS), which has both m...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Franco D,Meilhac SM,Christoffels VM,Kispert A,Buckingham M,Kelly RG

    更新日期:2006-06-15 00:00:00

  • Comparative biology of calcium signaling during fertilization and egg activation in animals.

    abstract::During animal fertilizations, each oocyte or egg must produce a proper intracellular calcium signal for development to proceed normally. As a supplement to recent synopses of fertilization-induced calcium responses in mammals, this paper reviews the spatiotemporal properties of calcium signaling during fertilization a...

    journal_title:Developmental biology

    pub_type: 杂志文章,评审


    authors: Stricker SA

    更新日期:1999-07-15 00:00:00

  • Osteogenic cell lineage analysis is facilitated by organ cultures of embryonic chick periosteum.

    abstract::Monoclonal antibodies against the surface of embryonic osteogenic cells (SB-1, SB-2, SB-3, and SB-5) have been used to characterize the sequence of transitions involved in the osteogenic cell lineage. In the present study, immunohistochemical analyses of the expression of osteogenic cell surface antigens in organ cult...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bruder SP,Caplan AI

    更新日期:1990-10-01 00:00:00

  • Segment-specific generation of Drosophila Capability neuropeptide neurons by multi-faceted Hox cues.

    abstract::In the Drosophila ventral nerve cord, the three pairs of Capability neuropeptide-expressing Va neurons are exclusively found in the second, third and fourth abdominal segments (A2-A4). To address the underlying mechanisms behind such segment-specific cell specification, we followed the developmental specification of t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Suska A,Miguel-Aliaga I,Thor S

    更新日期:2011-05-01 00:00:00

  • Effects of phlorizin and ouabain on the polarity of mouse 4-cell/16-cell stage blastomere heterokaryons.

    abstract::Cell polarity is thought to be required for the efficient production of nascent blastocoele fluid, which begins at the 16-cell stage of mouse preimplantation development. In this study the 4-cell/16-cell blastomere heterokaryon was used to test the hypothesis that solute transport across the apical membrane domain ind...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wiley LM,Obasaju MF

    更新日期:1989-06-01 00:00:00

  • Xenopus tropicalis nodal-related gene 3 regulates BMP signaling: an essential role for the pro-region.

    abstract::In vertebrates, nodal-related genes are crucial for specifying mesendodermal cell fates. Six nodal-related genes have been identified in Xenopus, but only one, nodal, has been identified in the mouse. The Xenopus nodal-related gene 3 (Xnr3), however, lacks the mesoderm-inducing activity of the other five nodal-related...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Haramoto Y,Tanegashima K,Onuma Y,Takahashi S,Sekizaki H,Asashima M

    更新日期:2004-01-01 00:00:00

  • Modulation of AP and DV signaling pathways by the homeotic gene Ultrabithorax during haltere development in Drosophila.

    abstract::Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mohit P,Makhijani K,Madhavi MB,Bharathi V,Lal A,Sirdesai G,Reddy VR,Ramesh P,Kannan R,Dhawan J,Shashidhara LS

    更新日期:2006-03-15 00:00:00

  • The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity.

    abstract::Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Heller RS,Stoffers DA,Liu A,Schedl A,Crenshaw EB 3rd,Madsen OD,Serup P

    更新日期:2004-04-01 00:00:00

  • Xenopus embryos lacking specific isoforms of the corepressor SMRT develop abnormal heads.

    abstract::The corepressor SMRT acts on a range of transcription factors, including the retinoid and thyroid hormone nuclear receptors. The carboxy-terminal region of SMRT contains CoRNR box motifs that mediate these interactions. We have shown, in Xenopus, that SMRT can exist as isoforms containing either two or three CoRNR box...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Malartre M,Short S,Sharpe C

    更新日期:2006-04-15 00:00:00

  • Strains matter: Success of murine in vitro spermatogenesis is dependent on genetic background.

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    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Portela JMD,Mulder CL,van Daalen SKM,de Winter-Korver CM,Stukenborg JB,Repping S,van Pelt AMM

    更新日期:2019-12-01 00:00:00

  • On the role of the 200-kDa neurofilament protein at the developing neuromuscular junction.

    abstract::To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Donahue SP,Wood JG,English AW

    更新日期:1988-11-01 00:00:00

  • The switch from cAMP-independent to cAMP-dependent arrest of meiotic prophase is associated with coordinated GPR3 and CDK1 expression in mouse oocytes.

    abstract::Mammalian oocytes are arrested in meiotic prophase from around the time of birth until just before ovulation. Following an extended period of growth, they are stimulated to mature to the metaphase II stage by a preovulatory luteinizing hormone (LH) surge that occurs with each reproductive cycle. Small, growing oocytes...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Firmani LD,Uliasz TF,Mehlmann LM

    更新日期:2018-02-01 00:00:00

  • Scheduled and unscheduled DNA synthesis during development in conjugating Tetrahymena.

    abstract::Autoradiography has been used to confirm and to extend previous microspectrophotometric studies (Doerder and DeBault, 1975) on the timing of DNA synthesis during conjugation in Tetrahymena thermophila. The majority of DNA synthesis occurs at the expected periods preceding gamete formation and the two postzygotic divis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Allis CD,Colavito-Shepanski M,Gorovsky MA

    更新日期:1987-12-01 00:00:00

  • Nerve and muscle development in paralysé mutant mice.

    abstract::Nerve and muscle development was studied in paralysé mutant mice. The mutant phenotype is first recognizable 6-7 days after birth (PN 6-PN 7) as cessation of muscle growth and weakness and incoordination of movement. Mutant animals die between 2 and 3 weeks of age. Muscle fibers from paralysé mutants had a unimodal di...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Blondet B,Duxson MJ,Harris AJ,Melki J,Guénet JL,Pinçon-Raymond M,Rieger F

    更新日期:1989-03-01 00:00:00

  • Generation and analysis of an improved Foxg1-IRES-Cre driver mouse line.

    abstract::Foxg1 expression is highly restricted to the telencephalon and other head structures in the early embryo. This expression pattern has been exploited to generate conditional knockout mice, based on a widely used Foxg1-Cre knock-in line (Foxg1(tm1(cre)Skm)), in which the Foxg1 coding region was replaced by the Cre gene....

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kawaguchi D,Sahara S,Zembrzycki A,O'Leary DDM

    更新日期:2016-04-01 00:00:00

  • Completion of Meiosis I of preovulatory oocytes and facilitation of preimplantation embryo development by glial cell line-derived neurotrophic factor.

    abstract::Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kawamura K,Ye Y,Kawamura N,Jing L,Groenen P,Gelpke MS,Rauch R,Hsueh AJ,Tanaka T

    更新日期:2008-03-01 00:00:00

  • A method for using direct injection of plasmid DNA to study cis-regulatory element activity in F0 Xenopus embryos and tadpoles.

    abstract::The ability to express exogenous reporter genes in intact, externally developing embryos, such as Xenopus, is a powerful tool for characterizing the activity of cis-regulatory gene elements during development. Although methods exist for generating transgenic Xenopus lines, more simplified methods for use with F0 anima...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wang C,Szaro BG

    更新日期:2015-02-01 00:00:00

  • Developmental patterns of expression and coexpression of myosin heavy chains in atria and ventricles of the avian heart.

    abstract::Monoclonal antibodies (mAbs), electrophoresis, immunoblotting, and immunohistochemistry were used to determine the molecular properties of cardiac myosin heavy chain (MHC) isoforms and the regions of the developing chicken heart in which they were expressed. Adult atria expressed three electrophoretically distinct MHC...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Evans D,Miller JB,Stockdale FE

    更新日期:1988-06-01 00:00:00

  • Keratin 10 gene expression during differentiation of mouse epidermis requires transcription factors C/EBP and AP-2.

    abstract::The epidermis forms a vital barrier composed of stratified keratinocytes and their differentiated products. One of these products, keratin K10, is critical to epidermal integrity, because mutations in k10 lead to abnormal blistering. For the normal expression of k10, differentiation-associated transcription factors C/...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Maytin EV,Lin JC,Krishnamurthy R,Batchvarova N,Ron D,Mitchell PJ,Habener JF

    更新日期:1999-12-01 00:00:00

  • Epigenetic discrimination by mouse metaphase II oocytes mediates asymmetric chromatin remodeling independently of meiotic exit.

    abstract::In mammalian fertilization, paternal chromatin is exhaustively remodeled, yet the maternal contribution to this process is unknown. To address this, we prevented the induction of meiotic exit by spermatozoa and examined sperm chromatin remodeling in metaphase II (mII) oocytes. Methylation of paternal H3-K4 and H3-K9 r...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yoshida N,Brahmajosyula M,Shoji S,Amanai M,Perry AC

    更新日期:2007-01-15 00:00:00

  • Isolation and partial characterization of a gene from trachea of Manduca sexta that requires and is negatively regulated by ecdysteroids.

    abstract::The molting cycle in insects is regulated by the fluctuating levels of ecdysteroids. Twenty-four hours prior to the pupal molt in Manduca sexta steroid titers are relatively high and they fall to very low levels at 4 hr before the molt. Several physiological events accompanying molts have been shown to require the ris...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Mészáros M,Morton DB

    更新日期:1994-04-01 00:00:00

  • Yolk proteins of Caenorhabditis elegans.

    abstract::A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Sharrock WJ

    更新日期:1983-03-01 00:00:00

  • Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development.

    abstract::Angiogenesis relies on specialized endothelial tip cells to extend toward guidance cues in order to direct growing blood vessels. Although many of the signaling pathways that control this directional endothelial sprouting are well known, the specific cellular mechanisms that mediate this process remain to be fully elu...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Yang H,Fang L,Zhan R,Hegarty JM,Ren J,Hsiai TK,Gleeson JG,Miller YI,Trejo J,Chi NC

    更新日期:2015-08-15 00:00:00

  • Normal newt limb regeneration requires matrix metalloproteinase function.

    abstract::Newts regenerate lost limbs through a complex process involving dedifferentiation, migration, proliferation, and redifferentiation of cells proximal to the amputation plane. To identify the genes controlling these cellular events, we performed a differential display analysis between regenerating and nonregenerating li...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Vinarsky V,Atkinson DL,Stevenson TJ,Keating MT,Odelberg SJ

    更新日期:2005-03-01 00:00:00

  • Ontogenesis of chick iris intrinsic muscles: evidence for a smooth-to-striated muscle transition.

    abstract::The iris sphincter muscle consists of striated muscle fibers in the adult chicken. The ontogenetic development of this muscle has been studied by immunocytochemistry, from Embryonic Day 7 to the time of hatching. The time course of expression of specific markers of either smooth or striated muscle, i.e., smooth and sk...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Volpe P,Biral D,Pizzo P,Salviati G,Margreth A

    更新日期:1993-10-01 00:00:00

  • Drosophila midgut morphogenesis requires the function of the segmentation gene odd-paired.

    abstract::Development of the Drosophila embryonic midgut is dependent on a number of genes, including tinman and bagpipe, which are required for formation of the visceral mesoderm, and the homeotic genes and their targets, which act locally in the visceral mesoderm to direct formation of specific midgut constrictions. Here we r...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Cimbora DM,Sakonju S

    更新日期:1995-06-01 00:00:00

  • Misexpression of noggin leads to septal defects in the outflow tract of the chick heart.

    abstract::BMP-2 and BMP-4 are known to be involved in the early events which specify the cardiac lineage. Their later patterns of expression in the developing mouse and chick heart, in the myocardium overlying the atrioventricular canal (AV) and outflow tract (OFT) cushions, also suggest that they may play a role in valvoseptal...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Allen SP,Bogardi JP,Barlow AJ,Mir SA,Qayyum SR,Verbeek FJ,Anderson RH,Francis-West PH,Brown NA,Richardson MK

    更新日期:2001-07-01 00:00:00

  • KLC3 is involved in sperm tail midpiece formation and sperm function.

    abstract::Kinesin light chain 3 (KLC3) is the only known kinesin light chain expressed in post-meiotic male germ cells. We have reported that in rat spermatids KLC3 associates with outer dense fibers and mitochondrial sheath. KLC3 is able to bind to mitochondria in vitro and in vivo employing the conserved tetratrico-peptide re...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Zhang Y,Ou Y,Cheng M,Saadi HS,Thundathil JC,van der Hoorn FA

    更新日期:2012-06-15 00:00:00