Expression of the Distalless-B gene in Ciona is regulated by a pan-ectodermal enhancer module.


:The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, which are also arranged in convergently transcribed bigene clusters. Sequence analysis of the C. intestinalis Dll-A-B cluster reveals a 378bp region upstream of Ci-Dll-B, termed B1, which is highly conserved with the corresponding region from the congener Ciona savignyi. The B1 element is necessary and sufficient to drive expression of a lacZ reporter gene in a pattern mimicking the endogenous expression of Ci-Dll-B at gastrula stages. This expression pattern which is specific to the entire animal hemisphere is activated preferentially in posterior, or b-lineage, cells by a central portion of B1. Expression in anterior, or a-lineage cells, can be activated by this central portion in combination with the distal part of B1. Anterior expression can also be activated by the central part of B1 plus both the proximal part of B1 and non-conserved sequence upstream of B1. Thus, cis-regulation of early Ci-Dll-B expression is activated by a required submodule in the center of B1, driving posterior expression, which works in combination with redundant submodules that respond to differentially localized anterior factors to produce the total animal hemisphere expression pattern. Interestingly, the intergenic region of the cluster, which is important for expression of the Dlx genes in vertebrates, does not have a specific activating function in the reporter genes tested, but acts as an attenuator in combination with upstream sequences.


Dev Biol


Developmental biology


Irvine SQ,Vierra DA,Millette BJ,Blanchette MD,Holbert RE




Has Abstract


2011-05-15 00:00:00














  • Proteolytic cleavage of the cell surface protein p160 is required for detachment of the fertilization envelope in the sea urchin.

    abstract::Sea urchin eggs secrete a serine protease activity, CGSP1, at fertilization that is essential for the block to polyspermy. Several targets of this proteolytic activity on the plasma membrane were identified here using a cell surface biotinylation approach. Amino acid microsequencing of one of these proteins led to the...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Haley SA,Wessel GM

    更新日期:2004-08-01 00:00:00

  • Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori.

    abstract::Hox genes are well-known master regulators in developmental morphogenesis along the anteroposterior axis of animals. However, the molecular mechanisms by which Hox proteins regulate their target genes and determine cell fates are not fully understood. The silk gland of Bombyx mori is a tubular tissue divided into seve...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kimoto M,Tsubota T,Uchino K,Sezutsu H,Takiya S

    更新日期:2014-02-01 00:00:00

  • Involvement of Pax6 and Otx2 in the forebrain-specific regulation of the vertebrate homeobox gene ANF/Hesx1.

    abstract::During early vertebrate development, ANF homeobox genes are expressed in the prospective forebrain. Their regulation is essential for correct morphogenesis and function of the prosencephalon. We identified a 1-kb fragment upstream of the chicken GANF gene sufficient to drive lacZ expression in the endogenous expressio...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Spieler D,Bäumer N,Stebler J,Köprunner M,Reichman-Fried M,Teichmann U,Raz E,Kessel M,Wittler L

    更新日期:2004-05-15 00:00:00

  • Roles of growth hormone and insulin-like growth factor 1 in mouse postnatal growth.

    abstract::To examine the relationship between growth hormone (GH) and insulin-like growth factor 1 (IGF1) in controlling postnatal growth, we performed a comparative analysis of dwarfing phenotypes manifested in mouse mutants lacking GH receptor, IGF1, or both. This genetic study has provided conclusive evidence demonstrating t...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Lupu F,Terwilliger JD,Lee K,Segre GV,Efstratiadis A

    更新日期:2001-01-01 00:00:00

  • Functional genomics identifies neural stem cell sub-type expression profiles and genes regulating neuroblast homeostasis.

    abstract::The Drosophila larval central brain contains about 10,000 differentiated neurons and 200 scattered neural progenitors (neuroblasts), which can be further subdivided into ~95 type I neuroblasts and eight type II neuroblasts per brain lobe. Only type II neuroblasts generate self-renewing intermediate neural progenitors ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Carney TD,Miller MR,Robinson KJ,Bayraktar OA,Osterhout JA,Doe CQ

    更新日期:2012-01-01 00:00:00

  • Targeted disruption of the cardiac troponin T gene causes sarcomere disassembly and defects in heartbeat within the early mouse embryo.

    abstract::Cardiac troponin T (cTnT) is a component of the troponin (Tn) complex in cardiac myocytes, and plays a regulatory role in cardiac muscle contraction by anchoring two other Tn components, troponin I (TnI) and troponin C, to tropomyosin (Tm) on the thin filaments. In order to determine the in vivo function of cTnT, we c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Nishii K,Morimoto S,Minakami R,Miyano Y,Hashizume K,Ohta M,Zhan DY,Lu QW,Shibata Y

    更新日期:2008-10-01 00:00:00

  • Characterization of an islet-activating protein-sensitive site in mouse sperm that is involved in the zona pellucida-induced acrosome reaction.

    abstract::Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Endo Y,Lee MA,Kopf GS

    更新日期:1988-09-01 00:00:00

  • Secondary heart field contributes myocardium and smooth muscle to the arterial pole of the developing heart.

    abstract::The arterial pole of the heart is the region where the ventricular myocardium continues as the vascular smooth muscle tunics of the aorta and pulmonary trunk. It has been shown that the arterial pole myocardium derives from the secondary heart field and the smooth muscle tunic of the aorta and pulmonary trunk derives ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Waldo KL,Hutson MR,Ward CC,Zdanowicz M,Stadt HA,Kumiski D,Abu-Issa R,Kirby ML

    更新日期:2005-05-01 00:00:00

  • Neural crest induction by the canonical Wnt pathway can be dissociated from anterior-posterior neural patterning in Xenopus.

    abstract::While Wnt signaling is known to be involved in early steps of neural crest development, the mechanism remains unclear. Because Wnt signaling is able to posteriorize anterior neural tissues, neural crest induction by Wnts has been proposed to be an indirect consequence of posteriorization of neural tissues rather than ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wu J,Yang J,Klein PS

    更新日期:2005-03-01 00:00:00

  • Severe developmental bone phenotype in ClC-7 deficient mice.

    abstract::Bone development is dependent on the functionality of three essential cell types: chondrocytes, osteoclasts and osteoblasts. If any of these cell types is dysfunctional, a developmental bone phenotype can result. The bone disease osteopetrosis is caused by osteoclast dysfunction or impaired osteoclastogenesis, leading...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Neutzsky-Wulff AV,Sims NA,Supanchart C,Kornak U,Felsenberg D,Poulton IJ,Martin TJ,Karsdal MA,Henriksen K

    更新日期:2010-08-15 00:00:00

  • The effect of agrin and laminin on acetylcholine receptor dynamics in vitro.

    abstract::Using optical imaging assays, we investigated the dynamics of acetylcholine receptors (AChRs) at laminin-associated clusters on cultured myotubes in the absence or presence of the nerve-derived clustering factor, agrin. Using fluorescence recovery after photobleaching (FRAP) on fluorescent bungarotoxin-labeled recepto...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Bruneau EG,Macpherson PC,Goldman D,Hume RI,Akaaboune M

    更新日期:2005-12-01 00:00:00

  • Complementary expression of IGF-II and IGFBP-5 during anterior pituitary development.

    abstract::The specification of the five individual hormone-secreting cell types in the anterior pituitary requires a series of sequential cell fate decisions. We have immortalized cells at several stages along this pathway of pituitary differentiation. Here, we present analysis of differences in gene expression between an anter...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Holley SJ,Hall SB,Mellon PL

    更新日期:2002-04-15 00:00:00

  • Bef medaka mutant reveals the essential role of c-myb in both primitive and definitive hematopoiesis.

    abstract::Vertebrate hematopoiesis is characterized by two evolutionally conserved phases of development, i.e., primitive hematopoiesis, which is a transient phenomenon in the early embryo, and definitive hematopoiesis, which takes place in the later stages. Beni fuji (bef) was originally isolated as a medaka mutant that has an...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Moriyama A,Inohaya K,Maruyama K,Kudo A

    更新日期:2010-09-15 00:00:00

  • spalt-induced specification of distinct dorsal and ventral domains is required for Drosophila tracheal patterning.

    abstract::Morphogenesis of the Drosophila tracheal system relies on different signalling pathways that have distinct roles in specifying both the migration of the tracheal cells and the particular morphological features of the primary branches. The current view is that the tracheal cells are initially specified as an equivalent...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Franch-Marro X,Casanova J

    更新日期:2002-10-15 00:00:00

  • Na/K-ATPase-mediated 86Rb+ uptake and asymmetrical trophectoderm localization of alpha1 and alpha3 Na/K-ATPase isoforms during bovine preattachment development.

    abstract::This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-su...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Betts DH,Barcroft LC,Watson AJ

    更新日期:1998-05-01 00:00:00

  • Roles of Polycomb group proteins Enhancer of zeste (E(z)) and Polycomb (Pc) during metamorphosis and larval leg regeneration in the flour beetle Tribolium castaneum.

    abstract::Many organisms both undergo dramatic morphological changes during post-embryonic development and also regenerate lost structures, but the roles of epigenetic regulators in such processes are only beginning to be understood. In the present study, the functions of two histone modifiers were examined during metamorphosis...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Chou J,Ferris AC,Chen T,Seok R,Yoon D,Suzuki Y

    更新日期:2019-06-01 00:00:00

  • The distribution of collapsin-1 mRNA in the developing chick nervous system.

    abstract::Collapsin-1 is a secreted glycoprotein that inhibits the extension of specific growth cones in vitro. It has been hypothesized to serve as a repulsive guidance cue for extending growth cones in vivo. Here we report the distribution of collapsin-1 message as demonstrated by in situ hybridization using digoxigenin-label...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Shepherd I,Luo Y,Raper JA,Chang S

    更新日期:1996-01-10 00:00:00

  • Mesenchymal Wnt signaling promotes formation of sternum and thoracic body wall.

    abstract::Midline defects account for approximately 5% of congenital abnormalities observed at birth. However, the molecular mechanisms underlying the formation of the ventral body wall are not well understood. Recent studies linked mutations in Porcupine-an O-acetyl transferase mediating Wnt ligand acylation-with defects in th...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Snowball J,Ambalavanan M,Cornett B,Lang R,Whitsett J,Sinner D

    更新日期:2015-05-15 00:00:00

  • Myogenesis and the intermediate filament protein, nestin.

    abstract::We show that the intermediate filament protein nestin is expressed in myogenic cells and that multiple mechanisms regulate nestin expression at different stages of myogenesis. Cultured embryonic, fetal, and neonatal mouse limb myoblasts initially expressed nestin in the absence of the four muscle regulatory factors (M...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kachinsky AM,Dominov JA,Miller JB

    更新日期:1994-09-01 00:00:00

  • Cusp patterning defect in Tabby mouse teeth and its partial rescue by FGF.

    abstract::Tabby is a mouse mutant characterized by deficient development of the ectodermal organs: teeth, hair, and a subset of glands. Ectodysplasin, the protein encoded by the Tabby gene, was recently identified as a novel TNF-like transmembrane protein but little is known about its function. We have examined the Tabby tooth ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Pispa J,Jung HS,Jernvall J,Kettunen P,Mustonen T,Tabata MJ,Kere J,Thesleff I

    更新日期:1999-12-15 00:00:00

  • Completion of Meiosis I of preovulatory oocytes and facilitation of preimplantation embryo development by glial cell line-derived neurotrophic factor.

    abstract::Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulate...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Kawamura K,Ye Y,Kawamura N,Jing L,Groenen P,Gelpke MS,Rauch R,Hsueh AJ,Tanaka T

    更新日期:2008-03-01 00:00:00

  • Production and utilization of growth factors related to fibroblast growth factor by embryonal carcinoma cells and their differentiated cells.

    abstract::Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Rizzino A,Kuszynski C,Ruff E,Tiesman J

    更新日期:1988-09-01 00:00:00

  • E-cadherin is required for cranial neural crest migration in Xenopus laevis.

    abstract::The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell-cell adhesion is known to play a crucial role in the directi...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Huang C,Kratzer MC,Wedlich D,Kashef J

    更新日期:2016-03-15 00:00:00

  • Regulation of M-phase progression in Chaetopterus oocytes by protein kinase C.

    abstract::We have examined the presence of protein kinase C in oocytes of Chaetopterus pergamentaceus and its role in the initiation of germinal vesicle breakdown (GVBD). First, we demonstrated that the oocytes contain a phospholipid- and calcium-dependent protein kinase, protein kinase C (PKC). Since PKC is the primary intrace...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Eckberg WR,Palazzo RE

    更新日期:1992-02-01 00:00:00

  • Tenascin in the developing chick visual system: distribution and potential role as a modulator of retinal axon growth.

    abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Perez RG,Halfter W

    更新日期:1993-03-01 00:00:00

  • Cavitation in the mouse preimplantation embryo: Na/K-ATPase and the origin of nascent blastocoele fluid.

    abstract::This study tested the proposition that Na/K-ATPase activity could be involved in the morphogenetic aspects of mouse blastocyst formation by facilitating the localization of certain organelles to apposed borders, the production of nascent blastocoele fluid, and cavitation. It was assumed that such Na/K-ATPase activity ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Wiley LM

    更新日期:1984-10-01 00:00:00

  • Prorenin receptor is critical for nephron progenitors.

    abstract::Deficient nephrogenesis is the major factor contributing to renal hypoplasia defined as abnormally small kidneys. Nephron induction during kidney development is driven by reciprocal interactions between progenitor cells of the cap mesenchyme (CM) and the ureteric bud (UB). The prorenin receptor (PRR) is a receptor for...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Song R,Preston G,Kidd L,Bushnell D,Sims-Lucas S,Bates CM,Yosypiv IV

    更新日期:2016-01-15 00:00:00

  • Comparative transcriptional profiling and evolutionary analysis of the GRAM domain family in eukaryotes.

    abstract::The GRAM domain was found in glucosyltransferases, myotubularins and other membrane-associated proteins. So far, functions for majority of these proteins are yet to be uncovered. In order to address the evolutionary and functional significance of this family members, we have performed a comprehensive investigation on ...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Jiang SY,Ramamoorthy R,Ramachandran S

    更新日期:2008-02-15 00:00:00

  • The role of Brn4/Pou3f4 and Pax6 in forming the pancreatic glucagon cell identity.

    abstract::Brain 4 (Brn4/Pou3f4) and Pax6 are POU-homeodomain and paired-homeodomain transcription factors, respectively, that are expressed in the brain and the glucagon-expressing cells in the pancreas. Brn4 expression begins at embryonic day 10 in the pancreas, just before pax6 and both appear in the glucagon immunoreactive c...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Heller RS,Stoffers DA,Liu A,Schedl A,Crenshaw EB 3rd,Madsen OD,Serup P

    更新日期:2004-04-01 00:00:00

  • Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.

    abstract::Specification of the primordial germ cells (PGCs) is essential for sexually reproducing animals. Although the mechanisms of PGC specification are diverse between organisms, the RNA binding protein Nanos is consistently required in the germ line in all species tested. How Nanos is selectively expressed in the germ line...

    journal_title:Developmental biology

    pub_type: 杂志文章


    authors: Oulhen N,Swartz SZ,Wang L,Wikramanayake A,Wessel GM

    更新日期:2019-08-01 00:00:00