Abstract:
:To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed the ADP ribosylation of a 40-kDa protein in crude membranes from rainbow trout postvitellogenic oocytes, and cholera toxin (CT) labeled several proteins, including a major protein with an apparent molecular weight of 43 kDa. The 40-kDa protein was recognized by an antibody against the alpha subunit of inhibitory G-proteins (Gi), whereas the 43-kDa protein was recognized by an antibody against the alpha subunit of stimulatory G-proteins (Gs). Treating the membrane fraction with 17 alpha,20 beta-DP decreased the PT-catalyzed ADP ribosylation of the 40-kDa protein. In contrast, there was no significant change in the CT-catalyzed ribosylation of the 43-kDa protein after exposure to 17 alpha,20 beta-DP. The specific binding of 17 alpha,20 beta-DP to membrane fractions was decreased by PT. 17 alpha,20 beta-DP binding was also inhibited by nonhydrolyzable GTP analogs such as guanosine 5'-O-(3-thiotriphosphate) (GTP gamma S) and guanylylimidodiphosphate (GppNHp), but not by either ATP or guanosine 5'-O-(2-thiodiphosphate) (GDP beta S). Scatchard analysis revealed that GppNHp induced a 3.8-fold increase in the dissociation constant without a significant change in the number of binding sites, suggesting that the GppNHp-induced decrease in 17 alpha,20 beta-DP binding is due to the decrease in binding affinity between 17 alpha,20 beta-DP and its receptors. We conclude that the PT-sensitive Gi is involved in the signal transduction pathway of 17 alpha,20 beta-DP in rainbow trout oocytes.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Yoshikuni M,Nagahama Ydoi
10.1006/dbio.1994.1341subject
Has Abstractpub_date
1994-12-01 00:00:00pages
615-22issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71341-8journal_volume
166pub_type
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