Abstract:
:The Drosophila abnormal wing discs (awd) gene encodes the subunit of a protein that has nucleoside diphosphate kinase (NDP kinase) activity. Null mutations of the awd gene cause lethality after puparium formation. Larvae homozygous for such mutations have small imaginal discs, lymph glands, and brain lobes. Neither the imaginal discs nor the ovaries from such null mutant larvae are capable of further growth or normal differentiation when transplanted into suitable host larvae. This null mutant phenotype can be entirely rescued by one copy of a transgene that has 750 bp of awd upstream regulatory DNA fused to a full-length awd cDNA. Tissue-specific expression of AWD protein from this rescue transgene is identical to tissue-specific expression of beta-galactosidase from a reporter transgene that has the same regulatory region fused to the bacterial lac Z gene. However, this rescue transgene or reporter transgene expression pattern is only a subset of the endogenous pattern of expression detected by either in situ hybridization or immunohistochemistry. This suggests that awd is normally expressed in some tissues where it is not required. The null mutant phenotype cannot be rescued at all by a transgene that has 750 bp of awd upstream regulatory DNA fused to a full-length awd cDNA with a mutation that eliminates NDP kinase activity by replacement of the active site histidine with alanine. This suggests that the enzymatic activity of the AWD protein is necessary for its biological function. The human genes nm23-H1 and nm23-H2 encode NDP kinase A and B subunits, respectively. The protein subunit encoded by either human nm23 gene is 78% identical to that encoded by the Drosophila awd gene. Transgenes that have the 750-bp awd upstream regulatory DNA fused to human nm23-H2 cDNA but not to nm23-H1 cDNA can rescue the imaginal disc phenotype and the zygotic lethality caused by homozygosis for an awd null mutation as efficiently as an awd transgene. However, rescue of female sterility requires twice as much nm23-H2 expression as awd expression. This implies that the enzymatic activity of the AWD protein is not sufficient for its biological function. The biological function may require nonconserved residues of the AWD protein that allow it to interact with other proteins.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Xu J,Liu LZ,Deng XF,Timmons L,Hersperger E,Steeg PS,Veron M,Shearn Adoi
10.1006/dbio.1996.0184subject
Has Abstractpub_date
1996-08-01 00:00:00pages
544-57issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90184-0journal_volume
177pub_type
杂志文章abstract::During endochondral bone formation, vascular invasion initiates the replacement of avascular cartilage by bone. We demonstrate herein that the cartilage-specific overexpression of VEGF-A(164) in mice results in the hypervascularization of soft connective tissues away from cartilage. Unexpectedly, perichondrial tissue ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.05.552
更新日期:2009-08-15 00:00:00
abstract::Cells from newborn rat hindlimb show greatly enhanced myogenicity when grown on surfaces coated with poly-L-lysine followed by laminin (PLL/Lam) instead of the collagens routinely used. Coating with poly-L-lysine (PLL) alone or with PLL followed by collagen does not enhance myogenicity. Both myogenic and nonmyogenic c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90327-7
更新日期:1987-07-01 00:00:00
abstract::Major insulin gene transcription factors, such as PDX-1 or NeuroD1, have equally important roles in pancreatic development and the differentiation of pancreatic endocrine cells. Previously, we identified and cloned another critical insulin gene transcription factor MafA (RIPE3b1) and reported that other Maf factors we...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.02.028
更新日期:2006-05-15 00:00:00
abstract::The mouse Otlx2 gene is a new member of the paired-like family of homeobox genes whose human homologue, RIEG, is involved in Rieger syndrome, an autosomal-dominant disorder. One of the cardinal features of Rieger syndrome is dental hypoplasia, indicating that Otlx2/RIEG activity is essential for normal tooth developme...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8672
更新日期:1997-09-15 00:00:00
abstract::The zebrafish fin is an excellent system to study the mechanisms of dermal bone patterning. Fin rays are segmented structures that form successive bifurcations both during ontogenesis and regeneration. Previous studies showed that sonic hedgehog (shha) may regulate regenerative bone patterning based on its expression ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.03.008
更新日期:2012-05-15 00:00:00
abstract::The dorsal-ventral axis of the Xenopus embryo is approximately determined by the point of sperm entry, which occurs randomly about the animal-vegetal axis of the egg. Two cytoplasmic components were studied whose spatial arrangements and local concentration patterns appear to reflect the forming dorsal-ventral axis. F...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90457-9
更新日期:1985-06-01 00:00:00
abstract::Isotopes of a number of crustacean myofibrillar proteins have been identified with sodium dodecyl sulfate-polyacrylamide electrophoresis, and their distribution in muscles of the snapping shrimp has been examined. Fast-slow differences in distribution have been observed for myosin light chains and tropomyosin. In cont...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90081-2
更新日期:1984-11-01 00:00:00
abstract::Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.040
更新日期:2007-05-01 00:00:00
abstract::Aurora A is a mitotic kinase essential for cell proliferation. In mice, ablation of Aurora A results in mitotic arrest and pre-implantation lethality, preventing studies at later stages of development. Here we report the effects of Aurora A ablation on embryo patterning at early post-implantation stages. Inactivation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.08.010
更新日期:2012-11-01 00:00:00
abstract::Because the formation of a mature nerve terminal requires the accumulation of large quantities of synaptic vesicles, the expression of synaptic vesicle proteins would be expected to correlate with synaptogenesis. However, previous studies have provided conflicting evidence on this point. We have examined the developme...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1244
更新日期:1993-09-01 00:00:00
abstract::The role of retrograde signaling between target cells and their presynaptic partners during early cell-cell interactions was examined in cell culture. Using time-lapse video microscopy and fura-2 calcium analysis, we followed contacts between presynaptic neurites of Helisoma motoneurons and muscle fibers dissociated f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1176
更新日期:1993-07-01 00:00:00
abstract::Cranial nerves VII, IX and X provide both gustatory (taste) and non-gustatory (touch, pain, temperature) innervation to the oral cavity of vertebrates. Gustatory neurons innervate taste buds and project centrally to the rostral nucleus of the solitary tract (NTS), whereas neurons providing general epithelial innervati...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.042
更新日期:2007-10-15 00:00:00
abstract::We have tested the role of proteoglycans in the development of feather pattern by culturing 7-day-old embryonic chick skins on medium containing para-nitrophenyl-beta-D-xyloside (2 mM). Xylosides compete with core proteins of proteoglycans by acting as exogenous acceptors for the synthesis of glycosaminoglycans leadin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90199-6
更新日期:1988-05-01 00:00:00
abstract::Interleukin-6 is a member of a class of hormone-like molecules termed cytokines. The actions of IL-6 are highly pleiotropic. In adults IL-6 functions as a major mediator of inflammatory responses as well as inducing the synthesis of acute phase proteins by the liver following infection or injury. Based on in vitro and...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/0012-1606(92)90173-e
更新日期:1992-06-01 00:00:00
abstract::To form a gonad, germ cells (GCs) and somatic gonadal precursor cells (SGPs) must migrate to the correct location in the developing embryo and establish the cell-cell interactions necessary to create proper gonad architecture. During gonad morphogenesis, SGPs send out cellular extensions to ensheath the individual GCs...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.04.027
更新日期:2012-07-15 00:00:00
abstract::Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.05.032
更新日期:2005-08-15 00:00:00
abstract::We have used an in vitro model to investigate the relationship between cell proliferation, cell maturation, and intercellular junctional changes that are seen during normal liver development. Chick liver fragments of embryonic stages 21, 28, and 39 were grown in organ culture in Medium 199 with 10% bovine serum albumi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90265-8
更新日期:1983-09-01 00:00:00
abstract::The germinal vesicle (GV) of starfish oocytes contains a factor which is required to drive the cytoplasmic cycle of the meiotic division. Biochemical investigation of this factor has been difficult due to the small quantities of obtainable GV materials. To overcome this, we have developed a mass-isolation procedure fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90187-l
更新日期:1992-06-01 00:00:00
abstract::Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1016
更新日期:1994-01-01 00:00:00
abstract::A modification of the scrape-loading/dye transfer technique was used to study gap junctional communication along the anterior-posterior (A-P) axis of embryonic chick wing buds at an early stage of development (stage 20/21) when positional values along the A-P axis are being specified. Extensive intercellular transfer ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90271-4
更新日期:1991-12-01 00:00:00
abstract::We have previously shown that the Caenorhabditis elegans gut-specific esterase gene (Ce-ges-1) has the unusual ability to be expressed in different modules of the embryonic digestive tract (anterior pharynx, posterior pharynx, and rectum) depending on sequence elements within the Ce-ges-1 promoter. In the present pape...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0442
更新日期:2001-11-15 00:00:00
abstract::Notch signaling is involved in a large range of developmental processes, and has been functionally implicated in body plan segmentation in two of the three diverse segmented taxa, the vertebrates and arthropods. Here we investigate expression of Notch, Delta, and hes gene homologues during larval and juvenile developm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.015
更新日期:2008-08-01 00:00:00
abstract::During Drosophila oogenesis, activation of Notch signaling in the follicular epithelium (FE) around stage 6 of oogenesis is essential for entry into the endocycle and a series of other changes such as cell differentiation and migration of subsets of the follicle cells. Notch induces the expression of zinc finger prote...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.04.024
更新日期:2014-08-01 00:00:00
abstract::The development of neural crest-derived melanocytes, as well as haematopoietic and germ cells, is affected by mutations of the Kit and Mgf genes, which lead to dominant spotting (W) or steel (Sl) phenotypes. Mgf codes for the ligand of the receptor tyrosine kinase encoded by the Kit locus. KitW-v, a point mutation exe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8738
更新日期:1997-12-01 00:00:00
abstract::To examine the possible role of homeo box genes in murine development we have studied the structure and expression pattern of Hox-2.5, a newly isolated homeo box gene that maps to the left end of the mouse Hox-2 locus on chromosome 11. The sequence of the Hox-2.5 homeo box has been determined. It is highly homologous ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90056-0
更新日期:1989-06-01 00:00:00
abstract::The development of the reproductive system in Caenorhabditis elegans is a well-established model system for patterning and organogenesis. We report the characterization of the cog-1 gene, mutations in which cause novel phenotypes in late patterning in vulval lineages, establishment of the vulva-uterine connection, dev...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0850
更新日期:2002-12-15 00:00:00
abstract::Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00255-0
更新日期:2003-08-15 00:00:00
abstract::Auxin is implicated throughout plant growth and development. Although the effects of this plant hormone have been recognized for more than a century, it is only in the past two decades that light has been shed on the molecular mechanisms that regulate auxin homeostasis, signaling, transport, crosstalk with other hormo...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2012.05.039
更新日期:2012-09-01 00:00:00
abstract::This study evaluated Na/K-ATPase alpha 1- and alpha 3-subunit isoform polypeptide expression and localization during bovine preattachment development. Na/K-ATPase cation transport activity from the one-cell to blastocyst stage was also determined by measuring ouabain-sensitive 86Rb+ uptake. Both alpha1- and alpha 3-su...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8874
更新日期:1998-05-01 00:00:00
abstract::Retinoic acid (RA) affects the response of many cells to growth factors, including the bone morphogenetic proteins (BMPs). The BMPs are members of the TGF-beta, family of growth factors, originally identified by their bone-inducing activities. Their widespread expression suggests many roles other than that in osteogen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0275
更新日期:1996-11-01 00:00:00