Abstract：
:Chondrocytes were isolated from the auricular cartilage of rabbits, aged 1 week to 30 months, and grown in short-term cell culture. The cells from the 1-week animals were small, polygonal, and mononucleated, while the chondrocytes from the older animals were larger, rounded, and frequently binucleated. The synthesis of proteoglycans, collagen, and elastin was determined by isotope incubation. Chemical characterization of the proteoglycans was also performed. The production of the matrix macromolecules showed a clear age dependence with peak synthesis occurring at different ages. Proteoglycans were actively synthesized by chondrocytes from all age groups with a broad maximum between 2 weeks and 5 months followed by a sharp decline to about 50% of the 1-week level at 12-30 months. Collagen synthesis peaked at 2 weeks, declining progressively thereafter to about 60% of the 1-week level at 30 months. Elastin synthesis was highest in the 1-week cultures and thereafter fell quickly to very low levels. In all age groups the chondrocytes synthesized predominantly cartilage-typic proteoglycans, i.e., large aggregate forming molecules containing chondroitin sulfate. Monomers and aggregates showed a size maximum at 2-8 weeks. The degree of sulfation of the chondroitin sulfate and the proportion of 6-sulfate increased with age. These findings support the concept of "age programs" for the biosynthesis and turnover of different matrix macromolecules.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Madsen K,Moskalewski S,von der Mark K,Friberg Udoi
10.1016/0012-1606(83)90311-1subject
Has Abstractpub_date
1983-03-01 00:00:00pages
63-73issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(83)90311-1journal_volume
96pub_type
杂志文章abstract：:The vertebrate pituitary gland is a key endocrine control organ that contains six distinct hormone secreting cell types. In this study, we analyzed the role of direct cell-to-cell Delta-Notch signaling in zebrafish anterior pituitary cell type specification. We demonstrate that initial formation of the anterior pituit...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.019
更新日期：2008-07-15 00:00:00
abstract：:In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1191
更新日期：1995-07-01 00:00:00
abstract：:Mesodermal cell migration during amphibian gastrulation is dependent on cellular interactions with fibronectin. One mechanism whereby cells bind fibronectin is through alpha v-containing integrin heterodimers. In order to investigate the role of alpha v in amphibian gastrulation, we have cloned the Pleurodeles homolog...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1212
更新日期：1995-08-01 00:00:00
abstract：:The embryonic cell lineage of Caenorhabditis elegans has been traced from zygote to newly hatched larva, with the result that the entire cell lineage of this organism is now known. During embryogenesis 671 cells are generated; in the hermaphrodite 113 of these (in the male 111) undergo programmed death and the remaind...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90201-4
更新日期：1983-11-01 00:00:00
abstract：:Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.048
更新日期：2008-01-15 00:00:00
abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:Hoxa-11 and Hoxd-11 are paralogous genes required for proper development of the vertebral column, the limbs, and the urogenital system. To further explore the functional relationship between these genes, as well as the potential rescue of one function by the other, we have introduced a Hoxd-11-expressing transgene int...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0137
更新日期：1996-06-15 00:00:00
abstract：:In previous studies we found that two glycoproteins of 220 and 180 kDa account for 80-90% of the material dissolved from the vitelline coat (VC) of Unio elongatulus egg (Focarelli and Rosati, 1993). The two glycoproteins were purified by electroelution and used to raise the corresponding polyclonal antibodies. Immunof...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1308
更新日期：1995-10-01 00:00:00
abstract：:Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct regions, exhibiting differences in m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.06.004
更新日期：2018-09-15 00:00:00
abstract：:In Dictyostelium discoideum cyclic AMP (cAMP) metabolism during macrocyst development, i.e., the sexual cycle of this organism, and in giant cells, i.e., fusion products from opposite mating-type cells, was investigated. The pattern of change in cAMP levels during macrocyst development differed considerably from that ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90104-0
更新日期：1984-08-01 00:00:00
abstract：:Mutations in the Pax6 gene disrupt telencephalic development, resulting in a thin cortical plate, expansion of proliferative layers, and the absence of the olfactory bulb. The primary defect in the neuronal cell population of the developing cerebral cortex was analysed by using mouse chimeras containing a mixture of w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00046-5
更新日期：2003-03-01 00:00:00
abstract：:In the developing vertebrate brain, growing axons establish a scaffold of axon tracts connected across the midline via commissures. We have previously identified a population of telencephalic neurons that express NOC-2, a novel glycoform of the neural cell adhesion molecule N-CAM that is involved in axon guidance in t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9549
更新日期：2000-01-15 00:00:00
abstract：:COUP-TFII (also known as Nr2f2), a member of the nuclear orphan receptor superfamily, is expressed in several regions of the central nervous system (CNS), including the ventral thalamus, hypothalamus, midbrain, pons, and spinal cord. To address the function of COUP-TFII in the CNS, we generated conditional COUP-TFII k...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.001
更新日期：2009-02-15 00:00:00
abstract：:Botryllus schlosseri is a colonial marine urochordate in which all adult organisms (called zooids) in a colony die synchronously by apoptosis (programmed cell death) in cyclical fashion. During this death phase called takeover, cell corpses within the dying organism are engulfed by circulating phagocytic cells. The "o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0772
更新日期：2002-09-15 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
abstract：:Completion of cytokinesis, abscission, has been studied little despite the intensive studies of the onset and contractile mechanism of the earlier phases of division. It has been well documented that microtubule (MT) disruption before furrow stimulation prevents furrowing, while MT disruption after furrow stimulation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9395
更新日期：1999-10-01 00:00:00
abstract：:To analyse cell migration and the differentiation potential of migratory stem cells in Hydractinia, we generated animals with an eGFP reporter gene stably expressed and transmitted via the germline. The transgene was placed under the control of two different actin promoters and the promoter of elongation factor-1α. On...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.017
更新日期：2010-12-01 00:00:00
abstract：:We have investigated oocyte development in Hydra vulgaris, a member of one of the oldest metazoan phyla. We show that oocyte determination involves a mechanism that establishes a subset of precursor interstitial cells competent to differentiate into oocytes. The oocyte is singled out from this subset and the competenc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9790
更新日期：2000-08-15 00:00:00
abstract：:Epithelial planar cell polarity (PCP) allows epithelial cells to coordinate their development to that of the tissue in which they reside. The mechanisms that impart PCP as well as effectors that execute the polarizing instructions are being sought in many tissues. We report that the epidermal epithelium of Drosophila ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.454
更新日期：2006-09-15 00:00:00
abstract：:The ancestral state of animal gastrulation and its bearing for our understanding of bilaterian evolution still is one of the most controversially discussed topics in the field of evolutionary and developmental biology. One hypothesis, the so-called amphistomy scenario, suggests the presence of a slit-like blastopore i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.04.014
更新日期：2017-07-01 00:00:00
abstract：:Vertebrate Cdx genes encode homeodomain transcription factors related to caudal in Drosophila. The murine Cdx homologues Cdx1, Cdx2 and Cdx4 play important roles in anterior-posterior patterning of the embryonic axis and the intestine, as well as axial elongation. While our understanding of the ontogenic programs requ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.09.034
更新日期：2012-01-01 00:00:00
abstract：:Exocytosis of cortical granules in mouse eggs is required to produce the zona pellucida block to polyspermy. In this study, we examined the role of microfilaments and microtubules in the regulation of cortical granule movement toward the cortex during oocyte maturation and anchoring of cortical granules in the cortex....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8945
更新日期：1998-08-01 00:00:00
abstract：:Malfunctions of motile cilia cause a variety of developmental defects and diseases in humans and animal model organisms. Defects include impaired mucociliary clearance of the airways, sperm immotility, hydrocephalus and organ laterality. Here, we characterize the evolutionary conserved Cfap43 gene by loss-of-function ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.010
更新日期：2020-03-15 00:00:00
abstract：:Metazoan replication-dependent histone mRNAs accumulate to high levels during S phase as a result of an increase in the rate of histone gene transcription, pre-mRNA processing, and mRNA stability at the G1-S transition. However, relatively little is known about the contribution of these processes to histone expression...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.06.019
更新日期：2004-10-01 00:00:00
abstract：:In mammalian fertilization, paternal chromatin is exhaustively remodeled, yet the maternal contribution to this process is unknown. To address this, we prevented the induction of meiotic exit by spermatozoa and examined sperm chromatin remodeling in metaphase II (mII) oocytes. Methylation of paternal H3-K4 and H3-K9 r...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.006
更新日期：2007-01-15 00:00:00
abstract：:Tubes with distinct shapes and sizes are critical for the proper function of many tubular organs. Here we describe a unique phenotype caused by the loss of a novel, evolutionarily-conserved, Drosophila Smad-like protein, Expansion. In expansion mutants, unicellular and intracellular tracheal branches develop bubble-li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.016
更新日期：2014-09-01 00:00:00
abstract：:One way to better understand the molecular mechanisms involved in the construction of a nervous system is to identify the downstream effectors of major regulatory proteins. We previously showed that Engrailed (EN) and Gooseberry-Neuro (GsbN) transcription factors act in partnership to drive the formation of posterior ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.10.018
更新日期：2017-12-15 00:00:00
abstract：:Despite differences in the formation of neural precursors, all arthropod species analyzed so far generate about 30 single precursors (insects/crustaceans) or precursor groups (chelicerates/myriapods) per hemi-segment. In Drosophila, each precursor has a distinct identity conferred by segment polarity and dorso-ventral...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.11.029
更新日期：2006-02-15 00:00:00
abstract：:In mammals, sex is determined in the bipotential embryonic gonad by a balanced network of gene actions which when altered causes disorders of sexual development (DSD, formerly known as intersex). In the XY gonad, presumptive Sertoli cells begin to differentiate when SRY up-regulates SOX9, which in turn activates FGF9 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.11.010
更新日期：2008-02-01 00:00:00
abstract：:Egg yolk phosvitin is one of the most highly phosphorylated extracellular matrix proteins known in nature with unique physico-chemical properties deemed to be critical during ex-vivo egg embryo development. We have utilized our unique live mouse calvarial bone organ culture models under conditions which dissociates th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.005
更新日期：2013-09-01 00:00:00
