Abstract：
:In mammals, sex is determined in the bipotential embryonic gonad by a balanced network of gene actions which when altered causes disorders of sexual development (DSD, formerly known as intersex). In the XY gonad, presumptive Sertoli cells begin to differentiate when SRY up-regulates SOX9, which in turn activates FGF9 and PGDS to maintain its own expression. This study identifies a new and essential component of FGF signaling in sex determination. Fgfr2 mutant XY mice on a mixed 129/C57BL6 genetic background had either normal testes, or developed ovotestes, with predominantly testicular tissue. However, backcrossing to C57BL6 mice resulted in a wide range of gonadal phenotypes, from hypoplastic testes to ovotestes with predominantly ovarian tissue, similar to Fgf9 knockout mice. Since typical male-specific FGF9-binding to the coelomic epithelium was abolished in Fgfr2 mutant XY gonads, these results suggest that FGFR2 acts as the receptor for FGF9. Pgds and SOX9 remained expressed within the testicular portions of Fgfr2 mutant ovotestes, suggesting that the Prostaglandin pathway acts independently of FGFR2 to maintain SOX9 expression. We could further demonstrate that double-heterozygous Fgfr2/Sox9 knockout mice developed ovotestes, demonstrating that both Fgfr2 and Sox9 can act as modifier intersex genes in the heterozygous state. In summary, we provide evidence that FGFR2 is important for male sex determination in mice, thereby rendering human FGFR2 a candidate gene for unsolved DSD cases such as 10q26 deletions.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Bagheri-Fam S,Sim H,Bernard P,Jayakody I,Taketo MM,Scherer G,Harley VRdoi
10.1016/j.ydbio.2007.11.010subject
Has Abstractpub_date
2008-02-01 00:00:00pages
71-83issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(07)01539-4journal_volume
314pub_type
杂志文章abstract：:Proper development of a multicellular organism relies on well-coordinated regulation of cell fate specification, cell proliferation and cell differentiation. The C. elegans postembryonic mesoderm provides a useful system for uncovering factors involved in these processes and for further dissecting their regulatory rel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.06.011
更新日期：2017-09-01 00:00:00
abstract：:Embryonic 4- to 15-day-old quail ciliary ganglia (CG) were grafted into the neural crest migration pathway of 2-day-old chick embryos at the adrenomedullary level of the neural axis. This back-transplantation results in dispersion of cells of the implanted ganglion, their migration in the host embryo, and subsequent p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90286-0
更新日期：1984-10-01 00:00:00
abstract：:The effect of epidermal growth factor (EGF) on cellular differentiation of the neonatal mouse mandibular incisor was examined autoradiographically using tritiated thymidine ([3H]TDR) and tritiated proline ([3H]PRO). On days 0 (day of birth), 1, and 2, EGF was administered (3 micrograms/g body wt) sc to neonates. Mice ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90506-9
更新日期：1987-12-01 00:00:00
abstract：:Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90160-i
更新日期：1992-07-01 00:00:00
abstract：:Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular ca...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9745
更新日期：2000-07-01 00:00:00
abstract：:Cell adhesion molecules (CAMs) perform numerous functions during neural development. An individual CAM can play different roles during each stage of neuronal differentiation; however, little is known about how such functional switching is accomplished. Here we show that Drosophila N-cadherin (CadN) is required at mult...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.04.006
更新日期：2012-06-15 00:00:00
abstract：:Foxg1 expression is highly restricted to the telencephalon and other head structures in the early embryo. This expression pattern has been exploited to generate conditional knockout mice, based on a widely used Foxg1-Cre knock-in line (Foxg1(tm1(cre)Skm)), in which the Foxg1 coding region was replaced by the Cre gene....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.02.011
更新日期：2016-04-01 00:00:00
abstract：:In Xenopus, the hatching and cement glands form at important boundaries in the head. We have examined induction of these glands in order to see the basis for this ectodermal patterning. Ectoderm can be induced to form hatching and cement glands by dorsal mesoderm and by the neural plate. Because the neural plate is su...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1183
更新日期：1993-07-01 00:00:00
abstract：:Dorsoventral specification of the zebrafish gastrula is governed by the functions of the dorsal shield, a region of the embryo functionally analogous to the amphibian Spemann organizer. We report that the bozozok locus encodes the transcription factor nieuwkoid/dharma, a homeobox gene with non-cell-autonomous organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9479
更新日期：1999-11-15 00:00:00
abstract：:The Drosophila ovarian germline stem cells (GSCs) constantly experience self-renewal and differentiation, ensuring the female fertility throughout life. The balance between GSC self-renewal and differentiation is exquisitely regulated by the stem cell niche, the stem cells themselves and systemic factors. Increasing e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.04.015
更新日期：2013-07-15 00:00:00
abstract：:Choroideremia (CHM) is a hereditary eye disease caused by mutations in the X-linked CHM gene. Disruption of the Chm gene in mice resulted in prenatal death of Chm-/Y males and Chm-/Chm+ females that had inherited the mutation from their mothers. Male chimeras and Chm+/Chm- females with paternal transmission of the mut...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.016
更新日期：2004-08-01 00:00:00
abstract：:A large subset of mammalian imprinted genes show extra-embryonic lineage (EXEL) specific imprinted expression that is restricted to placental trophectoderm lineages and to visceral yolk sac endoderm (ysE). Isolated ysE provides a homogenous in vivo model of a mid-gestation extra-embryonic tissue to examine the mechani...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.04.010
更新日期：2015-06-15 00:00:00
abstract：:Cell surface and cytoplasmic polarity is exhibited by the blastomeres of mouse preimplantation embryos following compaction at the 8-cell stage of cleavage. It has been hypothesized that cytoplasmic polarity is initiated by plasma membrane functions of polar blastomeres that are absent from apolar blastomeres. To test...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90433-2
更新日期：1988-11-01 00:00:00
abstract：:Normal animal development requires accurate cell divisions, not only in the early stages of rapid embryonic cleavages, but also in later developmental stages. The Caenorhabditis elegans unc-85 gene is implicated only in cell divisions that occur post-embryonically, primarily in terminal neuronal lineages. Variable pos...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.013
更新日期：2008-07-01 00:00:00
abstract：:Spinal cord regenerative ability is lost with development, but the mechanisms underlying this loss are still poorly understood. In chick embryos, effective regeneration does not occur after E13, when spinal cord injury induces extensive apoptotic response and tissue damage. As initial experiments showed that treatment...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.04.015
更新日期：2011-07-15 00:00:00
abstract：:For both the intricate morphogenetic layout of the sensory cells in the ear and the elegantly radial arrangement of the sensory neurons in the nose, numerous signaling molecules and genetic determinants are required in concert to generate these specialized neuronal populations that help connect us to our environment. ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2014.01.023
更新日期：2014-05-01 00:00:00
abstract：:We have tested the role of proteoglycans in the development of feather pattern by culturing 7-day-old embryonic chick skins on medium containing para-nitrophenyl-beta-D-xyloside (2 mM). Xylosides compete with core proteins of proteoglycans by acting as exogenous acceptors for the synthesis of glycosaminoglycans leadin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90199-6
更新日期：1988-05-01 00:00:00
abstract：:Xenopus laevis frogs from laboratory stocks normally lay eggs exhibiting extensive size variability. We find that these initial size differences subsequently affect the size of the embryos prior to the onset of growth, and the size of tadpoles during the growth period. Even though these tadpoles differ in size, their ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.03.014
更新日期：2020-06-15 00:00:00
abstract：:Conserved from Cnidarians to vertebrates, the dorsoventral (DV) axis is patterned by the Chordin-BMP pathway. However, the functions of the pathway's components are very different in different phyla. By modeling it is shown that many observations can be integrated by the assumption that BMP, acting as an inhibitory co...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.05.025
更新日期：2015-09-01 00:00:00
abstract：:Although round spermatid injection can be used to create progeny for males who do not produce mature sperm, the rate of successful embryogenesis after such procedures is significantly lower than that for similar procedures using mature spermatozoa. The mechanisms underlying this difference are unknown. In this study, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.026
更新日期：2006-01-01 00:00:00
abstract：:Spore germination is a defined developmental process that marks a critical point in the life cycle of Dictyostelium discoideum. Upon germination the environmental conditions must be conducive to cell growth to ensure survival of emerged amoebae. However, the signal transduction pathways controlling the various aspects...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8854
更新日期：1998-04-15 00:00:00
abstract：:Neurons of the dorsal hindbrain and spinal cord are central in receiving, processing and relaying sensory perception and participate in the coordination of sensory-motor output. Numerous cellular and molecular mechanisms that underlie neuronal development in both regions of the nervous system are shared. We discuss he...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.10.008
更新日期：2017-12-01 00:00:00
abstract：:Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00255-0
更新日期：2003-08-15 00:00:00
abstract：:The Jonah gene family consists of approximately 20 genes, distributed in small clusters at eight or more widely dispersed chromosomal sites. Gene clusters differ in the number of genes per cluster, ranging up to four, and in the arrangement of genes within clusters, which include direct repeats, inverted repeats, and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90038-7
更新日期：1985-04-01 00:00:00
abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.0025
更新日期：1995-11-01 00:00:00
abstract：:Formation of the adenohypophysis in mammalian embryos occurs via an invagination of the oral ectoderm to form Rathke's pouch, which becomes exposed to opposing dorsoventral gradients of signaling proteins governing specification of the different hormone-producing pituitary cell types. One signal promoting pituitary ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00124-0
更新日期：2003-02-01 00:00:00
abstract：:The Ci-Dll-B gene is an early regulator of ectodermal development in the ascidian Ciona intestinalis (Imai et al., 2006). Ci-Dll-B is located in a convergently transcribed bigene cluster with a tandem duplicate, Ci-Dll-A. This clustered genomic arrangement is the same as those of the homologous vertebrate Dlx genes, w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.02.009
更新日期：2011-05-15 00:00:00
abstract：:The categorical data set is an important data class in experimental biology and contains data separable into several mutually exclusive categories. Unlike measurement of a continuous variable, categorical data cannot be analyzed with methods such as the Student's t-test. Thus, these data require a different method of ...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2010.08.018
更新日期：2010-12-01 00:00:00
abstract：:The lateral line system displays highly divergent patterns in adult teleost fish. The mechanisms underlying this variability are poorly understood. Here, we demonstrate that the lateral line mechanoreceptor, the neuromast, gives rise to a series of accessory neuromasts by a serial budding process during postembryonic ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.02.017
更新日期：2010-04-15 00:00:00
abstract：:A genetically complex mutant, two-faced (tfd), which causes the production of extra eye and antennal tissue on the dorsal head cuticle, has been analyzed for connectivity to and projection patterns in the central brain of nerves derived from these extra structures. It has been found that the extra antennal nerves freq...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90370-7
更新日期：1985-01-01 00:00:00
