Abstract：
:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical removal of one olfactory bulb, the synaptic target of olfactory receptor neurons (ORNs) of the OE); and in primary cell cultures derived from embryonic mouse OE. In vivo, cells at all stages in the neuronal lineage--proliferating neuronal precursors, immature ORNs, and mature ORNs--displayed signs of apoptotic cell death; nonneuronal cells did not. Bulbectomy dramatically increased the number of apoptotic cells in the OE on the bulbectomized side. Shortly following bulbectomy, increased cell death involved neuronal cells of all stages. Later, cell death remained persistently elevated, but this was due to increased apoptosis by mature ORNs alone. In vitro, apoptotic death of both ORNs and their precursors could be inhibited by agents that prevent apoptosis in other cells: aurintricarboxylic acid (ATA), a membrane-permeant anlog of cyclic AMP (CPT-cAMP), and certain members of the neurotrophin family of growth factors (brain-derived neurotrophic factor, neurotrophin 3, and neurotrophin 5), although no neurotrophin was as effective at promoting survival as ATA or CPT-cAMP. Consistent with observed effects of neurotrophins, immunohistochemistry localized the neurotrophin receptors trkB and trkC to fractions of ORNs scattered throughout neonatal OE. These results suggest that apoptosis may regulate neuronal number in the OE at multiple stages in the neuronal lineage and that multiple factors-potentially including certain neurotrophins--may be involved in this process.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Holcomb JD,Mumm JS,Calof ALdoi
10.1006/dbio.1995.0025subject
Has Abstractpub_date
1995-11-01 00:00:00pages
307-23issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)70025-5journal_volume
172pub_type
杂志文章abstract：:Mutations in the NF1 gene may cause developmental abnormalities and the formation of a variety of tumors of neural crest origin in humans. The NF1 gene codes for a large protein, neurofibromin (nf), which is structurally and functionally related to yeast and human ras-GTPase-activating proteins (ras-GAPs). Recently, t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1052
更新日期：1994-02-01 00:00:00
abstract：:Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alterations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90320-9
更新日期：1986-10-01 00:00:00
abstract：:Pronuclear migration and formation of the first mitotic spindle depend upon assembly of a functional zygotic centrosome. For most animals, this involves both paternal and maternal contributions as sperm basal bodies are converted into centrosomes competent for microtubule nucleation through recruitment of egg proteins...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.001
更新日期：2004-01-15 00:00:00
abstract：:Cellular interactions with laminin are important for numerous morphogenetic events. In Xenopus, the first of these is neurulation. The integrin alpha6 subunit mediates an attachment of the cells of the neural plate to the underlying basal lamina. A disruption of this interaction results in embryos that fail to neurula...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9833
更新日期：2000-09-01 00:00:00
abstract：:This first study of the onset of spermatogenesis in the sea urchin, Strongylocentrotus purpuratus, was undertaken using individuals reared in the laboratory. Spermatogenesis commences about 11-12 months after metamorphosis in these animals. Bindin message accumulates in late spermatocytes and early spermatids which li...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90149-d
更新日期：1990-11-01 00:00:00
abstract：:Expression of cardiac troponin I (TnIcardiac) and slow skeletal troponin I (TnIslow) genes was analyzed at the mRNA and protein level in the developing rat heart. TnIslow mRNA was detectable by in situ hybridization in the embryonic cardiac tube as early as the 13-somite stage (Embryonic Day 10). In contrast, TnIcardi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1074
更新日期：1993-03-01 00:00:00
abstract：:It has long been hypothesized that in fishes the contents of cortical granules are involved in the hardening of egg envelope following fertilization. We previously purified the egg envelope hardening initiation factor from the exudates released from activated medaka (Oryzias latipes) eggs and tentatively termed this p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.09.016
更新日期：2012-12-15 00:00:00
abstract：:The spe-9 gene is required for fertility in Caenorhabditis elegans and encodes a sperm transmembrane protein with an extracellular domain (ECD) that contains 10 epidermal growth factor (EGF) repeats. Deletion analysis reveals that the EGF repeats and the transmembrane domain are required for fertilization. In contrast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.014
更新日期：2004-08-15 00:00:00
abstract：:Mitogen-activated protein kinases (MAPKs) are important regulators of many cellular processes. In mammalian testes, these kinases are involved in controlling cell division, differentiation, survival and death, and are therefore critical to spermatogenesis. Recent studies have also illustrated their involvement in junc...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2005.08.001
更新日期：2005-10-01 00:00:00
abstract：:To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.12.019
更新日期：2020-04-01 00:00:00
abstract：:Mutations in the Bone Morphogenetic Protein (BMP) pathway are associated with a range of defects in skeletal formation. Genetic analysis of BMP signaling requirements is complicated by the presence of three partially redundant BMPs that are required for multiple stages of limb development. We generated an inducible al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.07.003
更新日期：2014-09-15 00:00:00
abstract：:The levels of al-1 and al-2 transcripts change dramatically in response to light and development during the formation of Neurospora crassa asexual spores (conidia). al-1 and al-2 mRNAs accumulate throughout conidiation irrespective of lighting conditions initially at low levels. As conidiation proceeds, two increases ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1129
更新日期：1995-05-01 00:00:00
abstract：:Zebrafish heart regeneration relies on the capacity of cardiomyocytes to proliferate upon injury. To understand the principles of this process after cryoinjury-induced myocardial infarction, we established a spatio-temporal map of mitotic cardiomyocytes and their differentiation dynamics. Immunodetection of phosphohis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.12.002
更新日期：2015-03-01 00:00:00
abstract：:We wish to understand how organ-specific structures assemble during embryonic development. In the present paper, we consider what determines the subapical position of the terminal web in the intestinal cells of the nematode Caenorhabditis elegans. The terminal web refers to the organelle-depleted, intermediate filamen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.003
更新日期：2004-04-15 00:00:00
abstract：:The engrailed gene is well known from its role in segmentation and central nervous system development in a variety of species. In molluscs, however, engrailed is involved in shell formation. So far, it seemed that engrailed had been co-opted uniquely for this particular process in molluscs. Here, we show that, in the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0653
更新日期：2002-06-15 00:00:00
abstract：:Multiciliated cells (MCCs) differentiate hundreds of motile cilia that beat to drive fluid movement over various kinds of epithelia. In Xenopus, mice and human, the coiled-coil containing protein Mcidas (Mci) has been shown to be a key transcriptional regulator of MCC differentiation. We have examined Mci function in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.07.005
更新日期：2020-09-15 00:00:00
abstract：:Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9560
更新日期：2000-01-15 00:00:00
abstract：:The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(81)90400-0
更新日期：1981-06-01 00:00:00
abstract：:Eight series of mouse chimeras were produced by aggregating 8-cell embryos that differed at the Gpi-1s locus, encoding glucose phosphate isomerase (GPI-1). Chimeric blastocysts (Gpi-1sa/Gpi-1sa <--> Gpi-1sb/Gpi-1sb) were transferred to pseudopregnant females, which produced only GPI-1C enzyme. Quantitative electrophor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1062
更新日期：1995-03-01 00:00:00
abstract：:Cells that overexpress PKA as a consequence of carrying multiple copies of the gene encoding the catalytic subunit can be induced to sporulate when developing as single cells. A peptide phosphorylated by PKA, termed SDF-1, has recently been shown to stimulate this process (Anjard et al., 1997). Several genes have been...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8804
更新日期：1998-01-15 00:00:00
abstract：:Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.032
更新日期：2005-08-15 00:00:00
abstract：:Spermatogenesis is a complex process that requires coordinated proliferation and differentiation of male germ cells. The molecular events that dictate this process are largely unknown, but are likely to involve highly regulated transcriptional control. In this study, we investigate the contribution of chromatin associ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.07.006
更新日期：2012-09-15 00:00:00
abstract：:The proper removal of superfluous neurons through apoptosis and subsequent phagocytosis is essential for normal development of the central nervous system (CNS). During Drosophila embryogenesis, a large number of apoptotic neurons are efficiently engulfed and degraded by phagocytic glia. Here we demonstrate that glial ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.07.005
更新日期：2014-09-15 00:00:00
abstract：:The mouse prostate gland develops by branching morphogenesis from the urogenital epithelium and mesenchyme. Androgens and developmental factors, including FGF10 and SHH, promote prostate growth (Berman, D.M., Desai, N., Wang, X., Karhadkar, S.S., Reynon, M., Abate-Shen, C., Beachy, P.A., Shen, M.M., 2004. Roles for He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.08.018
更新日期：2005-12-15 00:00:00
abstract：:Recent studies with amoebae of the Myxomycetes Didymium iridis and Physarum polycephalum reveal that competence to undergo sexual fusion or to differentiate to plasmodia apogamically can be induced by a diffusible factor. Suspension cultures of Didymium amoebae have proven to be a good source of the inducer activity. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90337-3
更新日期：1984-06-01 00:00:00
abstract：:The PITX1 transcription factor is expressed during hindlimb development, where it plays a critical role in directing hindlimb growth and the specification of hindlimb morphology. While it is known that PITX1 regulates hindlimb formation, in part, through activation of the Tbx4 gene, other transcriptional targets remai...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.12.013
更新日期：2018-02-01 00:00:00
abstract：:The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90445-9
更新日期：1991-07-01 00:00:00
abstract：:In many non-mammalian vertebrates, adult dentitions result from cyclical rounds of tooth regeneration wherein simple unicuspid teeth are replaced by more complex forms. Therefore and by contrast to mammalian models, the numerical majority of vertebrate teeth develop shape during the process of replacement. Here, we ex...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.02.007
更新日期：2013-05-15 00:00:00
abstract：:Lens growth involves the proliferation of epithelial cells, followed by their migration to the equator region and differentiation into secondary fiber cells. It is widely accepted that fibroblast growth factor (FGF) signaling is required for the differentiation of lens epithelial cells into crystallin-rich fibers, but...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.05.029
更新日期：2012-08-01 00:00:00
abstract：:Although a large superfamily of G-protein-coupled receptors serves multiple functions, little is known about their functional activation during ontogeny. To examine the functional activation of the mu-opioid receptor (MOR) and the delta-opioid receptor (DOR) during development, sections of mouse embryos and fetuses fr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0855
更新日期：2003-01-01 00:00:00
