Abstract：
:Pronuclear migration and formation of the first mitotic spindle depend upon assembly of a functional zygotic centrosome. For most animals, this involves both paternal and maternal contributions as sperm basal bodies are converted into centrosomes competent for microtubule nucleation through recruitment of egg proteins. Nek2B is a vertebrate NIMA-related protein kinase required for centrosome assembly, as its depletion from egg extracts delays microtubule aster formation from sperm basal bodies. Using Xenopus as a model system, we now show that protein expression of Nek2B begins during mid-oogenesis and increases further upon oocyte maturation. This is regulated, at least in part, at the level of protein translation. Nek2B protein is weakly phosphorylated in mitotic egg extracts but its recruitment to the sperm basal body, which occurs independently of its kinase activity, stimulates its phosphorylation, possibly through sequestration from a phosphatase present in mitotic egg cytoplasm. Importantly, although Nek2B is not required to organize acentrosomal microtubule asters, we show that addition of either active or kinase-dead recombinant Nek2B can restore centrosome assembly in a dose-dependent manner to a depleted extract. These results support a model in which maternal Nek2B acts to promote assembly of a functional zygotic centrosome in a kinase-independent manner.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Twomey C,Wattam SL,Pillai MR,Rapley J,Baxter JE,Fry AMdoi
10.1016/j.ydbio.2003.10.001subject
Has Abstractpub_date
2004-01-15 00:00:00pages
384-98issue
2eissn
0012-1606issn
1095-564Xpii
S0012160603006109journal_volume
265pub_type
杂志文章abstract：:A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90049-0
更新日期：1983-02-01 00:00:00
abstract：:The Jonah gene family consists of approximately 20 genes, distributed in small clusters at eight or more widely dispersed chromosomal sites. Gene clusters differ in the number of genes per cluster, ranging up to four, and in the arrangement of genes within clusters, which include direct repeats, inverted repeats, and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90038-7
更新日期：1985-04-01 00:00:00
abstract：:Most metazoans are able to grow beyond a few hundred cells and to support differentiated tissues because they elaborate multicellular, epithelial tubes that are indispensable for nutrient and gas exchange. To identify and characterize the cellular behaviors and molecular mechanisms required for the morphogenesis of ep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.10.034
更新日期：2016-01-01 00:00:00
abstract：:We cloned Xenopus Strabismus (Xstbm), a homologue of the Drosophila planar cell or tissue polarity gene. Xstbm encodes four transmembrane domains in its N-terminal half and a PDZ-binding motif in its C-terminal region, a structure similar to Drosophila and mouse homologues. Xstbm is expressed strongly in the deep cell...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0673
更新日期：2002-07-01 00:00:00
abstract：:Retinoic acid (RA) plays a critical role in neural patterning and organogenesis in the vertebrate embryo. Here we characterize a mutant of the zebrafish named giraffe (gir) in which the gene for the RA-degrading enzyme Cyp26a1 is mutated. The gir mutant displayed patterning defects in multiple organs including the com...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.023
更新日期：2005-02-15 00:00:00
abstract：:The Drosophila brain is tracheated by the cerebral trachea, a branch of the first segmental trachea of the embryo. During larval stages the cerebral trachea splits into several main (primary) branches that grow around the neuropile, forming a perineuropilar tracheal plexus (PNP) at the neuropile surface. Five primary ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.09.022
更新日期：2007-02-01 00:00:00
abstract：:This study was undertaken to determine whether premigratory neural crest cells of the axolotl embryo differentiate autonomously into chromatophores, or whether stimuli from the environment, particularly from the extracellular matrix, are required for this process. Neural crest cells were excised from the dorsal part o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90168-5
更新日期：1986-02-01 00:00:00
abstract：:We have compared insulin binding by heart cells at 7 and 14 days of development. Species specificity, optimum pH, temperature relationships, and time to equilibrium for binding of insulin were the same in both 7 and 14-day systems. Curvilinear Scatchard plots for chicken insulin binding were demonstrated. Binding affi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(81)90410-3
更新日期：1981-06-01 00:00:00
abstract：:Intercellular communication mediated by gap junctions is important for tissue homeostasis in the avascular lens, and extensive areas of gap junctions form between fiber cells during fiber cell differentiation and lens development. We examined the role of the calcium-dependent cell adhesion molecule, N-cadherin, in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0237
更新日期：1996-10-10 00:00:00
abstract：:We have examined the expression of mRNAs encoding five major neurotransmitter-synthesizing enzymes in MAH cells, a clonal cell line derived by retroviral immortalization of a rat embryonic sympathoadrenal progenitor cell. These mRNAs include tyrosine hydroxylase (TH), choline acetyltransferase (ChAT), tryptophan hydro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90313-r
更新日期：1991-11-01 00:00:00
abstract：:The ubiquitin ligase Smurf1 can target a handful of signaling proteins for ubiquitin-mediated proteasomal destruction or functional modification, including TGF-beta receptors, Smads, transcription factors, RhoA and MEKK2. Smurf1 was initially implicated in BMP pathway regulation in embryonic development, but its poten...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.009
更新日期：2006-11-15 00:00:00
abstract：:Chromatin insulators orchestrate gene transcription during embryo development and cell differentiation by stabilizing interactions between distant genomic sites. Mutations in genes encoding insulator proteins are generally lethal, making in vivo functional analyses of insulator proteins difficult. In Drosophila, howev...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.03.024
更新日期：2015-07-01 00:00:00
abstract：:Thyroid hormone (TH) induces dramatic skeletal and tissue remodeling of the anuran head and body at metamorphosis. The expression pattern of TH up-regulated genes has been correlated with tissues that either grow or resorb at metamorphosis. Whereas the expression of the thyroid hormone receptors in Xenopus laevis tadp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8975
更新日期：1998-11-01 00:00:00
abstract：:P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90202-t
更新日期：1990-04-01 00:00:00
abstract：:It has been suggested that the mesonephros has a role in normal limb development. This hypothesis was directly tested by removing the mesonephros adjacent to the presumptive limb region of stage 12-18 chick embryos using microsurgery or laser ablation. The experimental manipulation resulted in reduced limb outgrowth o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90230-e
更新日期：1992-05-01 00:00:00
abstract：:Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alterations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90320-9
更新日期：1986-10-01 00:00:00
abstract：:The organization of the embryonic neural plate requires coordination of multiple signal transduction pathways, including fibroblast growth factors (FGFs), bone morphogenetic proteins (BMPs), and WNTs. Many studies have suggested that a critical component of this process is the patterning of posterior neural tissues by...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.019
更新日期：2009-09-01 00:00:00
abstract：:lilliputian (lilli), the sole Drosophila member of the FMR2/AF4 (Fragile X Mental Retardation/Acute Lymphoblastic Leukemia) family of transcription factors, is widely expressed with roles in segmentation, cellularization, and gastrulation during early embryogenesis with additional distinct roles at later stages of emb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.027
更新日期：2007-01-15 00:00:00
abstract：:The survival effects of brain-derived neurotrophic factor (BDNF) on the ganglion cells of the chick retina were studied in vitro at different embryonic ages. We found these effects to be strongly age-dependent: at E5, when the first ganglion cell axons have crossed the optic chiasm, but not yet reached the tectum, gan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90256-x
更新日期：1989-12-01 00:00:00
abstract：:The embryonic dorsal vessel in Drosophila possesses anteroposterior polarity and is subdivided into two chamber-like portions, the aorta in the anterior and the heart in the posterior. The heart portion features a wider bore as compared with the aorta and develops inflow valves (ostia) that allow the pumping of hemoly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0839
更新日期：2002-11-15 00:00:00
abstract：:Runx2-Cbfa1, a Runt transcription factor, plays important roles during skeletal development. It is required for differentiation and function of osteoblasts. In its absence, chondrocyte hypertrophy is severely impaired and there is no vascularization of cartilage templates during skeletal development. These tissue-spec...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.003
更新日期：2004-06-15 00:00:00
abstract：:In the developing retina, as in other regions of the CNS, neural progenitors give rise to individual cell types during discrete temporal windows. Pax6 is expressed in retinal progenitor cells (RPCs) throughout the course of retinogenesis, and has been shown to be required during early retinogenesis for generation of m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.09.030
更新日期：2017-12-01 00:00:00
abstract：:Cortical granules of eggs contain a population of heterogeneous proteins that are sequestered selectively within the cortical granule during oogenesis and that participate in the block to polyspermy during the fertilization reaction. To begin to understand the targeting mechanism of proteins to this organelle, we have...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1033
更新日期：1995-01-01 00:00:00
abstract：:The basic helix-loop-helix transcription factor Twist1 plays an essential role in mesenchymal cell populations during embryonic development and in pathological disease. Remodeling of the cardiac outflow tract (OFT) into the functionally separate aortic arch and pulmonary trunk is dependent upon the dynamic, coordinate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.037
更新日期：2008-08-01 00:00:00
abstract：:John W. Saunders, Jr. made seminal discoveries unveiling how chick embryos develop their limbs. He discovered the apical ectodermal ridge (AER), the zone of polarizing activity (ZPA), and the domains of interdigital cell death within the developing limb and determined their function through experimental analysis. Thes...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.11.005
更新日期：2017-09-15 00:00:00
abstract：:The origin and development of mouse kidney vasculature were examined in chorioallantoic grafts of early kidney rudiments and of experimentally induced explants of separated metanephric mesenchymes. Whole kidney rudiments developed into advanced stages, expressed the segment-specific antigenic markers of tubules and th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90180-x
更新日期：1983-04-01 00:00:00
abstract：:Hox genes are crucial players in the generation and pattering of the vertebrate trunk and posterior body during embryogenesis. Their initial expression takes place shortly after the establishment of the primitive streak, in the posterior-most part of the mouse embryo and is a determinant step for setting up the defini...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.02.009
更新日期：2017-08-15 00:00:00
abstract：:Sex in the nematode Caenorhabditis elegans is normally determined by the X chromosome to autosome (X:A) ratio, with XX hermaphrodites and XO males. Previous work has shown that a set of at least four autosomal genes (her-1, tra-2, tra-3, and tra-1) is signaled by the X:A ratio and appears to act in a regulatory pathwa...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90077-0
更新日期：1984-11-01 00:00:00
abstract：:Treatment of metaphase II-arrested hamster eggs with activators of protein kinase C has been reported to promote resumption of the cell cycle, second polar body emission, and pronucleus formation (G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco, 1993, Dev. Biol. 156, 94-106). In contrast, we have not obse...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1233
更新日期：1995-08-01 00:00:00
abstract：:Cardiomyocyte differentiation overall has been analyzed in vivo and in vitro at the molecular level by homologous recombination, gene mutation studies, and by transgenics; however, the roles of many signal transduction mechanisms that drive this differentiation process are still not fully understood. One set of signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00080-5
更新日期：2003-03-15 00:00:00
