Abstract:
:P19 embryonal carcinoma cells are multipotential stem cells that differentiate into striated muscle as well as some other cell types when aggregated and exposed to dimethyl sulfoxide (DMSO). Immunofluorescence experiments using monospecific antibodies indicated that the majority of muscle cells were mononucleate and contained four myosin isoforms normally found in cardiac muscle; atrial and ventricular myosin heavy chains, ventricular myosin light chain 1, and atrial myosin light chain 2. Northern blot analysis of RNA isolated from differentiating cultures indicated that cardiac actin and skeletal actin mRNAs were expressed at similar levels and with identical kinetics during the differentiation of P19-derived myocytes. These results demonstrate that most of the P19-derived myocytes are of the cardiac type and suggest that they closely resemble the cells of the early embryonic myocardium.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Rudnicki MA,Jackowski G,Saggin L,McBurney MWdoi
10.1016/0012-1606(90)90202-tsubject
Has Abstractpub_date
1990-04-01 00:00:00pages
348-58issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(90)90202-Tjournal_volume
138pub_type
杂志文章abstract::The cellular mechanisms underlying the amazing ability of sea cucumbers to regenerate their autotomized intestines have been widely described by us and others. However, the signaling pathways that control these mechanisms are unknown. Previous studies have shown that Wnt homologs are upregulated during early intestina...
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
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