Abstract:
:The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular matrix components that are required for fertilization and perhaps for early development. This study uses immunofluorescence and immunogold techniques with two novel monoclonal antibodies against proteins found in egg yolk and cortical granules to study the localizations of these antigens during early starfish development. Partial biochemical characterizations using the anti-yolk antibody have revealed that there are a family of structurally related proteins in oocyte yolk granules and that while the molecular compositions of the yolk proteins change during embryogenesis, their depletion is not significant until the larval stage, suggesting these proteins are not required for early development. In addition, a large immunoreactive protein has been found in the intestine and coelomic fluid, suggesting that, as in other species, the starfish yolk proteins may be derived from a large precursor, such as vitellogenin. Analysis of the anti-cortical granule antibody has revealed that a 120-kDa antigen is stored in antibody has revealed that a 120-kDa antigen is stored in cortical granules of unfertilized eggs. Upon egg activation, the cortical granules located in the peripheral egg cytoplasm undergo exocytosis, and the 120-kDa antigen is released into the perivitelline space. However, other granules, which are also labeled by this antibody, remain dispersed throughout the egg cytoplasm and are still present in the early gastrula, where they appear to contribute to the extracellular matrices of the developing embryo. This suggests that starfish cortical granules play a dual function: At fertilization, where they help create a block to polyspermy, and in embryonic development, where they secrete extracellular matrix components.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Reimer CL,Crawford BJdoi
10.1006/dbio.1995.1040subject
Has Abstractpub_date
1995-02-01 00:00:00pages
439-57issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)71040-8journal_volume
167pub_type
杂志文章abstract::Joint and skeletal development is highly regulated by extracellular matrix (ECM) proteoglycans, of which chondroitin sulfate proteoglycans (CSPGs) are a major class. Despite the requirement of joint CSPGs for skeletal flexibility and structure, relatively little is understood regarding their role in establishing joint...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.01.005
更新日期:2012-03-15 00:00:00
abstract::Cardiomyocyte differentiation overall has been analyzed in vivo and in vitro at the molecular level by homologous recombination, gene mutation studies, and by transgenics; however, the roles of many signal transduction mechanisms that drive this differentiation process are still not fully understood. One set of signal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00080-5
更新日期:2003-03-15 00:00:00
abstract::Three unrelated chromosome 2q14.1-14.2 region translocations caused the split hand/foot limb malformation development in humans by an unknown mechanism. Their etiology was described by the autosomal dominant inheritance with incomplete penetrance genetic model although authors stated, "the understanding of the genotyp...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2015.10.013
更新日期:2015-12-01 00:00:00
abstract::Vertebrate retinal progenitor cells (RPCs) undergo a robust proliferative expansion to produce enough cells for the retina to form appropriately. Vsx2 (formerly Chx10), a homeodomain protein expressed in RPCs, is required for sufficient proliferation to occur. Sonic Hedgehog protein (SHH), secreted by retinal ganglion...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.055
更新日期:2008-05-15 00:00:00
abstract::Cellular interactions with laminin are important for numerous morphogenetic events. In Xenopus, the first of these is neurulation. The integrin alpha6 subunit mediates an attachment of the cells of the neural plate to the underlying basal lamina. A disruption of this interaction results in embryos that fail to neurula...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9833
更新日期:2000-09-01 00:00:00
abstract::There is a natural curiosity about how organisms give rise to offspring like themselves through a series of reproducible developmental events and how, once mature, these offspring mate and continue the process giving rise the next generation. In the mid-1800s investigators started using gametes and embryos to explore ...
journal_title:Developmental biology
pub_type: 历史文章,杂志文章
doi:10.1016/j.ydbio.2011.06.021
更新日期:2011-10-15 00:00:00
abstract::We have investigated the accumulation and adenylation of the maternal mRNA during oogenesis in the oocytes of the marine worm Urechis caupo. The analysis, using in vitro translation and cDNA probes to assay for specific mRNAs, demonstrates that different maternal mRNAs accumulate with different patterns during oogenes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90347-7
更新日期:1986-09-01 00:00:00
abstract::Mature hamster eggs exhibit repetitive increases in the intracellular Ca2+ concentration ([Ca2+]i) at fertilization, caused by inositol 1,4,5-trisphosphate (InsP3)-induced Ca2+ release (IICR) from stores. Oscillating Ca2+ rises also occurred in inseminated immature oocytes at the germinal vesicle (GV) stage but the pe...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1059
更新日期:1993-03-01 00:00:00
abstract::Myogenesis in the embryo and the adult mammal consists of a highly organized and regulated sequence of cellular processes to form or repair muscle tissue that include cell proliferation, migration, and differentiation. Data from cell culture and in vivo experiments implicate both FGFs and HGF as critical regulators of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0416
更新日期:2001-11-01 00:00:00
abstract::The germ-line cells of many animals possess a characteristic cytoplasmic structure termed nuage or germinal granules. In mice, nuage that is prominent in postnatal male germ cells is also called intermitochondrial cement or chromatoid bodies. TDRD1/MTR-1, which contains Tudor domain repeats, is a specific component of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.046
更新日期:2007-01-01 00:00:00
abstract::Although the development of several of the voltage-dependent currents in embryonic amphibian myocytes has been described, the overall muscle electrical development, particularly the relative times of expression of different voltage-dependent currents, has not been addressed in a single study under one set of condition...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90043-g
更新日期:1992-11-01 00:00:00
abstract::The segmentation of somites from the paraxial mesoderm is a crucial event in vertebrate embryonic development; however, the mechanisms underlying this process are not well understood. In a yeast two-hybrid screen we have identified the novel basic-helix-loop-helix (bHLH) protein cMeso-1 which is expressed in the preso...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8919
更新日期:1998-07-15 00:00:00
abstract::Here we describe a novel set of peptidergic neurons conserved throughout all developmental stages in the Drosophila central nervous system (CNS). We show that a small complement of 28 apterous-expressing cells (Ap-let neurons) in the ventral nerve cord (VNC) of Drosophila larvae co-express numerous gene products. The ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.015
更新日期:2004-05-01 00:00:00
abstract::CREB-2 (also called ATF4, TAXREB67, or C/ATF) is an evolutionarily conserved member of the CREB/ATF family of basic-leucine zipper transcription factors. CREB-2 is expressed ubiquitously in the adult mouse and can function as both a transcriptional activator and a repressor. However, little was understood about the no...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9699
更新日期:2000-06-01 00:00:00
abstract::Vertebrate hematopoiesis is characterized by two evolutionally conserved phases of development, i.e., primitive hematopoiesis, which is a transient phenomenon in the early embryo, and definitive hematopoiesis, which takes place in the later stages. Beni fuji (bef) was originally isolated as a medaka mutant that has an...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.06.031
更新日期:2010-09-15 00:00:00
abstract::Connective tissue outgrowths of neonatal muscle onto a substratum of bone matrix differentiate into cartilage in response to a bone morphogenetic protein (BMP). The BMP can be separated from bone matrix by extraction with 4 M guanidine hydrochloride (GuHCl) or degraded in situ by endogenous proteolytic enzymes to deac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90339-9
更新日期:1988-12-01 00:00:00
abstract::We demonstrate that interspecific sequence conservation can provide a systematic guide to the identification of functional cis-regulatory elements within a large expanse of genomic DNA. The test was carried out on the otx gene of Strongylocentrotus purpuratus. This gene plays a major role in the gene regulatory networ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0618
更新日期:2002-06-01 00:00:00
abstract::The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. XFLRT3, a Nodal/TGFbeta target, regulates cell adhesion and modulates FGF signalling during Xenopus gast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.03.021
更新日期:2008-06-01 00:00:00
abstract::To examine the possible role of homeo box genes in murine development we have studied the structure and expression pattern of Hox-2.5, a newly isolated homeo box gene that maps to the left end of the mouse Hox-2 locus on chromosome 11. The sequence of the Hox-2.5 homeo box has been determined. It is highly homologous ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90056-0
更新日期:1989-06-01 00:00:00
abstract::Animal development is controlled by gene regulation networks that are composed of sequence-specific transcription factors (TF) and cell signaling molecules (ST). Although housekeeping genes have been reported to show clustering in the animal genomes, whether the genes comprising a given regulatory network are physical...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.009
更新日期:2008-04-15 00:00:00
abstract::In the present study, we have investigated the role of tyrosine kinase activity during early retinal development in Xenopus laevis. The protein tyrosine kinase (PTK) inhibitors lavendustin A and genistein were used to determine the possible role of tyrosine kinase activity during retinal development in vivo and in vit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.10.006
更新日期:2004-02-01 00:00:00
abstract::The Drosophila melanogaster ventral nerve cord derives from neural progenitor cells called neuroblasts. Individual neuroblasts have unique gene expression profiles and give rise to distinct clones of neurons and glia. The specification of neuroblast identity provides a cell intrinsic mechanism which ultimately results...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.01.016
更新日期:2006-04-15 00:00:00
abstract::Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9474
更新日期:1999-11-15 00:00:00
abstract::Sonic hedgehog is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb. The signaling pathway mediating the transduction of the signal is still poorly understood. In Drosophila several genes are known to act downstream of hedgehog, the fly homolog of Sonic hedgehog. An importan...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0300
更新日期:1996-11-25 00:00:00
abstract::The relative transcription of three unlinked actin genes of the sea urchin Strongylocentrotus purpuratus was measured in isolated nuclei, at several stages during embryonic development. Transcription of two cytoskeletal actin genes, CyI and CyIIIa, was first detected in 64-128 cell embryos. At the early stages, the Cy...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90473-8
更新日期:1987-11-01 00:00:00
abstract::Oligodendrocyte precursor cells (OPCs) can be differentiated in culture into either oligodendrocytes or type-2 astrocytes (2As), depending on the culture conditions. Whereas oligodendrocyte development can occur in the absence of inducing signals, 2A development apparently cannot. Fetal calf serum (FCS) and bone morph...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.11.013
更新日期:2004-03-01 00:00:00
abstract::Completion of cytokinesis, abscission, has been studied little despite the intensive studies of the onset and contractile mechanism of the earlier phases of division. It has been well documented that microtubule (MT) disruption before furrow stimulation prevents furrowing, while MT disruption after furrow stimulation ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9395
更新日期:1999-10-01 00:00:00
abstract::The Foxl1 gene, which encodes a winged helix transcriptional regulator, is expressed in the mesenchymal layer of developing and mature gastrointestinal tract. Foxl1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(02)00088-x
更新日期:2003-03-15 00:00:00
abstract::Deficient nephrogenesis is the major factor contributing to renal hypoplasia defined as abnormally small kidneys. Nephron induction during kidney development is driven by reciprocal interactions between progenitor cells of the cap mesenchyme (CM) and the ureteric bud (UB). The prorenin receptor (PRR) is a receptor for...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.11.024
更新日期:2016-01-15 00:00:00
abstract::The distribution of the extracellular matrix protein tenascin was studied in the developing chick visual system to determine its possible regulatory role in retinotectal development. Little tenascin was present in the retinal optic fiber layer, and the optic nerve and tract, but was abundant in the stratum opticum of ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1076
更新日期:1993-03-01 00:00:00