Abstract：
:Although the development of several of the voltage-dependent currents in embryonic amphibian myocytes has been described, the overall muscle electrical development, particularly the relative times of expression of different voltage-dependent currents, has not been addressed in a single study under one set of conditions. We have found that, in mesoderm isolated and cultured from neurula stage embryos, myocytes are identifiable before they express voltage-gated currents. These ionic currents are absent from all Xenopus mesodermal cells during the late gastrula/early neurula stages of embryonic development. At about the time of first somite segregation an inward rectifier K+ current is expressed in some myocytes, followed within 2 hr by a delayed rectifier K+ current. The density of both currents increases fourfold over the next 24 hr in culture. A Na+ current is not expressed in large numbers of myocytes until late in this culture period, at about the time that a slow Ca2+ current appears. Under our culture conditions the myocytes have a very low chloride conductance. A fast inactivating component to the outward K+ current is expressed in all myocytes by 24 hr in culture. In some experiments we dissociated embryos at later times and made recordings when all previously isolated myocytes expressed currents. In the late dissociations, most myocytes did not express currents, but developed them after a short period in culture. Because we have evidence that in vivo development is more closely approximated by the early dissociations, these results suggest that dissociation causes some degree of dedifferentiation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Spruce AE,Moody WJdoi
10.1016/0012-1606(92)90043-gsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
11-22issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(92)90043-Gjournal_volume
154pub_type
杂志文章abstract：:Human MusTRD1alpha1 was isolated as a result of its ability to bind a critical element within the Troponin I slow upstream enhancer (TnIslow USE) and was predicted to be a regulator of slow fiber-specific genes. To test this hypothesis in vivo, we generated transgenic mice expressing hMusTRD1alpha1 in skeletal muscle....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.019
更新日期：2006-05-01 00:00:00
abstract：:Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0718
更新日期：2002-08-15 00:00:00
abstract：:The subdivision of the developing field by region-specific expression of genes encoding transcription factors is an essential step during appendage development in arthropod and vertebrates. In Drosophila leg development, the distal-most region (pretarsus) is specified by the expression of homeobox genes, aristaless an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.12.005
更新日期：2005-03-15 00:00:00
abstract：:Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9474
更新日期：1999-11-15 00:00:00
abstract：:As the germ band shortens in Drosophila melanogaster embryos, cell shape changes cause segments to narrow anteroposteriorly and to lengthen dorsoventrally. One of the genes required for this retraction process is the hindsight (hnt) gene. hnt encodes a nuclear Zinc-finger protein that is expressed in the extraembryoni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9409
更新日期：1999-10-01 00:00:00
abstract：:Developing cardiac myocytes divide a limited number of times before they stop and terminally differentiate, but the mechanism that stops their division is unknown. To help study the stopping mechanism, we defined conditions under which embryonic rat cardiac myocytes cultured in serum-free medium proliferate and exit t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9524
更新日期：1999-12-15 00:00:00
abstract：:The direct flight muscles (DFMs) of Drosophila allow for the fine control of wing position necessary for flight. In DWnt-2 mutant flies, certain DFMs are either missing or fail to attach to the correct epithelial sites. Using a temperature-sensitive allele, we show that DWnt-2 activity is required only during pupation...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0572
更新日期：2002-03-15 00:00:00
abstract：:The Rho family of small GTPases has been implicated in many neurological disorders including mental retardation, but whether they are involved in primary microcephaly (microcephalia vera) is unknown. Here, we examine the role of Rac1 in mammalian neural progenitors and forebrain development by a conditional gene-targe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.10.023
更新日期：2009-01-01 00:00:00
abstract：:The prostate gland develops from the urogenital sinus by a testosterone-dependent process of ductal morphogenesis. Sonic hedgehog (Shh) is expressed in the urogenital sinus epithelium and the time course of expression coincides with the formation of the main prostatic ducts. Expression is most abundant in the lumen of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9229
更新日期：1999-05-01 00:00:00
abstract：:A fundamental feature of cartilage differentiation in the developing limb is the formation of a prechondrogenic cell condensation. An apparently similar process of prechondrogenic cell aggregation occurs in micromass cultures of limb bud mesenchyme with the formation of cellular aggregates which often differentiate in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90314-9
更新日期：1987-08-01 00:00:00
abstract：:Patterning of the early neural tube is achieved in part by the inductive signals, which arise from neuroepithelial signaling centers. The zona limitans intrathalamica (ZLI) is a neuroepithelial domain in the alar plate of the diencephalon which separates the prethalamus from the thalamus. The ZLI has recently been con...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.031
更新日期：2005-08-15 00:00:00
abstract：:The mammalian Cone-rod homeobox (Crx) gene is a divergent member of the Otx gene family known to be involved in differentiation and survival of retinal photoreceptors and photoentrainment of circadian rhythms. Zebrafish have two genes in the Otx5/crx orthology class, and we previously showed that crx can transactivate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.037
更新日期：2004-05-01 00:00:00
abstract：:Canonical Wnt signalling is known to be involved in the regulation of differentiation and proliferation in the context of endodermal organogenesis. Wnt mediated beta-catenin activation is understood to be modulated by secreted Frizzled-related proteins, such as XsFRP5, which is dynamically expressed in the prospective...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.03.004
更新日期：2009-05-15 00:00:00
abstract：:Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.05.019
更新日期：2017-08-01 00:00:00
abstract：:About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8972
更新日期：1998-09-01 00:00:00
abstract：:Homeotic genes in the Antennapedia Complex of Drosophila specify identity of the posterior head segments; the labial segment requires Sex combs reduced (Scr) for proper development, Deformed (Dfd) specifies maxillary and mandibular identity, and labial is necessary for intercalary segment identity. Although mutations ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0290
更新日期：1996-11-25 00:00:00
abstract：:The elongation of embryo and tissue is a key morphogenetic event in embryogenesis and organogenesis. Notochord, a typical chordate organ, undergoes elongation to perform its regulatory roles and to form the structural support in the embryo. Notochord elongation is morphologically similar across all chordates, but asci...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.009
更新日期：2019-04-15 00:00:00
abstract：:Somatic embryogenesis is one of the best examples of the remarkable developmental plasticity of plants, in which committed somatic cells can dedifferentiate and acquire the ability to form an embryo and regenerate an entire plant. In Arabidopsis thaliana, the shoot apices of young seedlings have been reported as an al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.04.023
更新日期：2018-10-01 00:00:00
abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:A chronic neuromuscular blockade during those embryonic stages when naturally occurring spinal motoneuron death occurs, results in the prevention of this cell loss. The excess motoneurons are maintained as long as the neuromuscular blockade is continued; once embryonic neuromuscular activity resumes, however, the exce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90114-3
更新日期：1984-01-01 00:00:00
abstract：:Global silencing of transcriptional activity in the oocyte genome occurs just before the resumption of meiosis and is a crucial developmental transition at the culmination of oogenesis. Transcriptionally quiescent oocytes rely on stored maternal transcripts to sustain the completion of meiosis, fertilization, and earl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.028
更新日期：2004-11-15 00:00:00
abstract：:Vertebrate neural tube formation involves two distinct morphogenetic events--convergent extension (CE) driven by mediolateral cell intercalation, and bending of the neural plate driven largely by cellular apical constriction. However, the cellular and molecular biomechanics of these processes are not understood. Here,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.12.009
更新日期：2009-03-15 00:00:00
abstract：:In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.005
更新日期：2019-04-15 00:00:00
abstract：:TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.025
更新日期：2014-12-01 00:00:00
abstract：:Directed cell movement is integral to both embryogenesis and hematopoiesis. In the adult, the chemokine family of secreted proteins signals migration of hematopoietic cells through G-coupled chemokine receptors. We detected embryonic expression of chemokine receptor messages by RT-PCR with degenerate primers at embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9405
更新日期：1999-09-15 00:00:00
abstract：:Hox genes of the Abdominal-B (Abd-B) class regulate gonadal development in diverse metazoans. Here we have investigated the role of the Abd-B homolog egl-5 in C. elegans gonadal development. Previous work showed that egl-5 is required male-specifically in the gonad and that mutant gonads are highly dysgenic and possib...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.516
更新日期：2010-08-15 00:00:00
abstract：:MicroRNAs (miRNAs) are post-transcriptional regulators of growth and development in both plants and animals. Flowering is critical for the reproduction of angiosperms. Flower development entails the transition from vegetative growth to reproductive growth, floral organ initiation, and the development of floral organs....
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2013.05.009
更新日期：2013-08-15 00:00:00
abstract：:The Hippo tumor suppressor pathway plays many fundamental cell biological roles during animal development. Two central players in controlling Hippo-dependent gene expression are the TEAD transcription factor Scalloped (Sd) and its transcriptional co-activator Yorkie (Yki). Hippo signaling phosphorylates Yki, thereby b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.022
更新日期：2019-11-01 00:00:00
abstract：:The midbrain-hindbrain boundary (MHB) acts as a local organizer in the development of the CNS in vertebrates. Previously, we identified an MHB-specific bHLH-WRPW transcriptional repressor gene, Xenopus Hes-related 1 (XHR1), which is initially expressed in the presumptive MHB (pre-MHB) region at the early gastrula stag...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.04.020
更新日期：2005-07-01 00:00:00
abstract：:Salivary gland formation in the Drosophila embryo is dependent on the homeotic gene Sex combs reduced (Scr). When Scr function is missing, salivary glands do not form, and when SCR is expressed everywhere in the embryo, salivary glands form in new places. Scr is normally expressed in all the cells that form the saliva...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9560
更新日期：2000-01-15 00:00:00
