Abstract：
:Canonical Wnt signalling is known to be involved in the regulation of differentiation and proliferation in the context of endodermal organogenesis. Wnt mediated beta-catenin activation is understood to be modulated by secreted Frizzled-related proteins, such as XsFRP5, which is dynamically expressed in the prospective liver/ventral pancreatic precursor cells during late neurula stages, becoming liver specific at tailbud stages and shifting to the posterior stomach/anterior duodenum territory during tadpole stages of Xenopus embryogenesis. These expression characteristics prompted us to analyse the function of XsFRP5 in the context of endodermal organogenesis. We demonstrate that XsFRP5 can form a complex with and inhibit a multitude of different Wnt ligands, including both canonical and non-canonical ones. Knockdown of XsFRP5 results in transient pancreatic hypoplasia as well as in an enlargement of the stomach. In VegT-injected animal cap explants, XsFRP5 can induce expression of exocrine but not endocrine pancreatic marker genes. Both, its expression characteristics as well as its interactions with XsFRP5, define Wnt2b as a putative target for XsFRP5 in vivo. Knockdown of Wnt2b results in a hypoplastic stomach as well as in hypoplasia of the pancreas. On the basis of these findings we propose that XsFRP5 exerts an early regulatory function in the specification of the ventral pancreas, as well as a late function in controlling stomach size via inhibition of Wnt signalling.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Damianitsch K,Melchert J,Pieler Tdoi
10.1016/j.ydbio.2009.03.004subject
Has Abstractpub_date
2009-05-15 00:00:00pages
327-37issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(09)00171-7journal_volume
329pub_type
杂志文章abstract：:There has been a resurgence of interest in comparative embryology. It is now important to be able to compare gene expression in different species at similar developmental stages. One phenomenon which may make it difficult to compare embryos in this way is heterochrony--a change in developmental timing during evolution...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1006/dbio.1995.8041
更新日期：1995-12-01 00:00:00
abstract：:Paracrine signaling mediated by FGF-10 and the FGF-R2IIIb receptor is required for formation of the lung. To determine the temporal requirements for FGF signaling during pulmonary morphogenesis, Sprouty-4 (Spry-4), an intracellular FGF receptor antagonist, was expressed in epithelial cells of the fetal lung under cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00106-4
更新日期：2003-06-01 00:00:00
abstract：:To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90260-0
更新日期：1987-04-01 00:00:00
abstract：:The location, timing and intensity of Nodal signalling are all critical for proper patterning of the vertebrate embryo. Genetic evidence from mouse and zebrafish indicates that EGF-CFC family members are essential for Nodal ligands to signal. However, the Xenopus EGF-CFC, FRL1, has been implicated in Wnt signalling an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.006
更新日期：2006-04-15 00:00:00
abstract：:Notch signaling is involved in a large range of developmental processes, and has been functionally implicated in body plan segmentation in two of the three diverse segmented taxa, the vertebrates and arthropods. Here we investigate expression of Notch, Delta, and hes gene homologues during larval and juvenile developm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.015
更新日期：2008-08-01 00:00:00
abstract：:Specification of the trophectoderm (TE) and inner cell mass (ICM) lineages in the mouse blastocyst correlates with cell position, as TE derives from outer cells whereas ICM from inner cells. Differences in position are reflected by cell polarization and Hippo signaling. Only in outer cells, the apical-basal cell polar...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.023
更新日期：2014-10-01 00:00:00
abstract：:An abundant phosphoprotein with an apparent molecular mass of 65 kDa (p65) has been identified that is enriched in developing new macronuclei (or anlagen) isolated from the holotrichous ciliate, Tetrahymena thermophila. During early stages of macronuclear development, p65 is actively synthesized and deposited into you...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1264
更新日期：1994-10-01 00:00:00
abstract：:Insulin-producing beta-cells are present as single cells or in small clusters distributed throughout the pancreas of the Xenopus laevis tadpole. During metamorphic climax when the exocrine pancreas dedifferentiates to progenitor cells, the beta-cells undergo two changes. Insulin mRNA is down regulated at the beginning...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.038
更新日期：2009-04-15 00:00:00
abstract：:The development of the action potential and responses to neurotransmitters have been described for a population of embryonic spinal neurons developing in vivo. A comparable pattern is seen for spinal neurons developing in dissociated cell culture. The impulse appears very early in this developmental sequence, and the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90065-4
更新日期：1984-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90094-0
更新日期：1984-08-01 00:00:00
abstract：:Immunohistochemical analysis of myosin heavy chain (MHC) isoform expression in perinatal and adult rat diaphragm muscles was performed with antibodies which permitted the identification of all known MHC isoforms found in typical rat muscles. Isoform switching, leading to the emergence of the adult phenotype, was more ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90473-g
更新日期：1991-03-01 00:00:00
abstract：:During early development, GATA factors have been shown to be important for key events of coronary vasculogenesis, including formation of the epicardium. Myocardial GATA factors are required for coronary vascular (CV) formation; however, the role of epicardial localized GATAs in this process has not been addressed. The...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.033
更新日期：2014-02-01 00:00:00
abstract：:The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present st...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90188-7
更新日期：1989-10-01 00:00:00
abstract：:Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1016
更新日期：1994-01-01 00:00:00
abstract：:An antiserum (anti-H2) directed at the second helix of the helix-loop-helix (HLH) protein MyoD1 reacts with a protein expressed during avian cardiac myocyte differentiation. Indirect immunohistochemical whole mount staining with anti-H2 detected a protein expressed in stage 11 hearts, but not in hearts of older embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1088
更新日期：1993-04-01 00:00:00
abstract：:This study describes the temporal pattern of posterior positional identity in mouse limb bud cells. To do this wedges of tissue from the posterior edge of mouse limb buds at various stages (limb stages: Wanek et al., 1989b. J. Exp. Zool. 249, 41-49) were grafted to the anterior edge of a host chick embryo wing bud. Gr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90306-n
更新日期：1991-10-01 00:00:00
abstract：:The Drosophila salivary gland is a simple tubular organ derived from a contiguous epithelial primordium, which is established by the activities of the homeodomain-containing proteins Sex combs reduced (SCR), Extradenticle (EXD), and Homothorax (HTH). EGF signaling along the ventral midline specifies the salivary duct ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00140-4
更新日期：2003-06-15 00:00:00
abstract：:The vertebrate cranial base is a complex structure composed of bone, cartilage and other connective tissues underlying the brain; it is intimately connected with development of the face and cranial vault. Despite its central importance in craniofacial development, morphogenesis and tissue origins of the cranial base h...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.016
更新日期：2008-10-01 00:00:00
abstract：:The thyroid gland synthesizes thyroxine (T4), which passes through the larval tadpole's circulatory system. The enzyme type II iodothyronine deiodinase (D2) converts thyroxine (T4) to the active hormone 3,5,3'-triiodothyronine (T3) in peripheral tissues. An early response to thyroid hormone (TH) in the Xenopus laevis ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.005
更新日期：2004-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90339-9
更新日期：1988-12-01 00:00:00
abstract：:The structure of the three polypeptide chains of the laminin subunits and the number of glycosylation sites in each polypeptide chain were determined using peptide mapping by high-performance liquid chromatography. Analysis of the [35S]methionine-labeled underglycosylated laminin isolated from tunicamycin (TM)-treated...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90367-6
更新日期：1983-08-01 00:00:00
abstract：:The spe-9 gene is required for fertility in Caenorhabditis elegans and encodes a sperm transmembrane protein with an extracellular domain (ECD) that contains 10 epidermal growth factor (EGF) repeats. Deletion analysis reveals that the EGF repeats and the transmembrane domain are required for fertilization. In contrast...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.014
更新日期：2004-08-15 00:00:00
abstract：:Transgenic mice expressing stabilized beta-catenin in neural progenitors develop enlarged brains resulting from increased progenitor expansion. To more precisely define beta-catenin regulation of progenitor fate, we employed a conditional transgenic approach to delete the beta-catenin regulatory domain from neural pro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.013
更新日期：2007-09-15 00:00:00
abstract：:The regeneration of lost body parts is a fascinating phenomenon exhibited by some multicellular organisms. In social amoebae, such as Dictyostelium discoideum, the pseudoplasmodium is a temporary migratory multicellular structure with high regeneration ability. It consists of future stalk cells (prestalk cells) at the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.09.014
更新日期：2020-01-01 00:00:00
abstract：:Insulin-like growth factor-1 (IGF-1) is believed to play a role in the regulation of brain growth. The identity of cells responsible for its synthesis in the immature brain, however, has not been established. To identify potential sites of IGF-1 synthesis, in situ hybridization has been utilized to localize IGF-1 mRNA...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(05)80021-1
更新日期：1991-09-01 00:00:00
abstract：:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90275-0
更新日期：1983-09-01 00:00:00
abstract：:Cadherin-11 (cad-11) is a novel member of the cadherin family of cell adhesion molecules, having recently been identified by means of the polymerase chain reaction. To study the function and expression of this molecule, we cloned mouse, cad-11 cDNA. Transfection of L cells with cDNA led them to acquire a typical cadhe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1149
更新日期：1995-05-01 00:00:00
abstract：:Complement components have been implicated in a wide variety of functions including neurogenesis, proliferation, cell migration, differentiation, cancer, and more recently early development and regeneration. Following our initial observations indicating that C3a/C3aR signaling induces chick retina regeneration, we ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.05.019
更新日期：2017-08-01 00:00:00
abstract：:Actin dynamics are necessary at multiple steps in the formation of multinucleated muscle cells. BAR domain proteins can regulate actin dynamics in several cell types, but have been little studied in skeletal muscle. Here, we identify novel functions for the N-BAR domain protein, Bridging integrator 3 (Bin3), during my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.004
更新日期：2013-10-01 00:00:00
abstract：:Previous studies from this laboratory (M. Bronner-Fraser (1985). J. Cell Biol. 101, 610) have demonstrated that an antibody to a cell surface receptor complex caused alterations in avian neural crest cell migration. Here, these observations are extended to examine the distribution and persistency of injected antibody,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90320-9
更新日期：1986-10-01 00:00:00
