Abstract：
:A fundamental feature of cartilage differentiation in the developing limb is the formation of a prechondrogenic cell condensation. An apparently similar process of prechondrogenic cell aggregation occurs in micromass cultures of limb bud mesenchyme with the formation of cellular aggregates which often differentiate into cartilage nodules. We have investigated the process of aggregate formation in micromass culture using chimaeric mixtures of potentially chondrogenic and nonchondrogenic cell types. Two systems were studied: mixtures of distal and proximal limb mesenchyme cells and mixtures of distal limb cells with avian tendon fibroblasts. In both cases cultures of varying proportions of each cell type have been prepared. The results demonstrate that aggregate formation in vitro is the consequence of a cell sorting process which can involve prechondrogenic cells of widely different spatial origins within the developing limb. This contrasts with in vivo prechondrogenic condensation in which there is no evidence of cell sorting (Searls, R.L. (1967), J. Exp. Zool. 166, 39-50). However, our findings do indicate that cell surface differences occur in apparently undifferentiated limb mesenchyme. The results also suggest that mesenchymal cell aggregates must achieve a threshold size before chondrogenesis can proceed. In addition, the results show that under some culture conditions nonchondrogenic cells will form aggregates.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Cottrill CP,Archer CW,Wolpert Ldoi
10.1016/0012-1606(87)90314-9subject
Has Abstractpub_date
1987-08-01 00:00:00pages
503-15issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(87)90314-9journal_volume
122pub_type
杂志文章abstract：:Full-grown Xenopus oocytes, Stage VI (1200-1300 microns), undergo meiotic maturation when exposed to progesterone. Smaller stage IV (800 microns) and stage V (1000 microns) oocytes remain in prophase arrest when exposed to this steroid. The larger stage VI oocytes undergo an intracellular alkalization from 7.2 to 7.6,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90288-4
更新日期：1984-10-01 00:00:00
abstract：:During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein dis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.02.019
更新日期：2010-05-01 00:00:00
abstract：:Peptide growth factors can initiate changes in cell fate in Xenopus ectodermal explants and induce the formation of mesoderm. Marker genes expressed in mesoderm allow the analysis of whether, or how much, induction has occurred, but do not tell us what molecules are involved in carrying out the response. In this repor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0158
更新日期：1996-07-10 00:00:00
abstract：:Stable subdivision of Drosophila limbs into Anterior (A) and Posterior (P) compartments is a consequence of asymmetric signaling by Hedgehog (Hh) from P to A cells. The activity of the homeodomain protein Engrailed (En) in P cells has been reported to help to generate this asymmetry by inducing the expression of hedge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.006
更新日期：2009-03-15 00:00:00
abstract：:The Endo16 gene codes for an RGD-containing calcium-binding protein that is found on the basal surfaces and in the extracellular matrix of cells of the invaginating archenteron during sea urchin gastrulation. Previously, we have shown that Endo16 is a single copy gene and we have determined the coding sequence and ana...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0247
更新日期：1996-10-10 00:00:00
abstract：:Mutations in the Bone Morphogenetic Protein (BMP) pathway are associated with a range of defects in skeletal formation. Genetic analysis of BMP signaling requirements is complicated by the presence of three partially redundant BMPs that are required for multiple stages of limb development. We generated an inducible al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.07.003
更新日期：2014-09-15 00:00:00
abstract：:Bone morphogenetic protein-4 (BMP-4) induces epidermis and represses neural fate in Xenopus ectoderm. Our previous findings implicate p42 Erk MAP kinase (MAPK) in the response to neural induction. We have examined the effects of BMP-4 on MAPK activity in gastrula ectoderm. Expression of a dominant negative BMP-4 recep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0338
更新日期：2001-08-15 00:00:00
abstract：:In the embryonic mouse retina, retinoic acid (RA) is unevenly distributed along the dorsoventral axis: RA-rich zones in dorsal and ventral retina are separated by a horizontal RA-poor stripe that contains the RA-inactivating enzyme CYP26A1. To explore the developmental role of this arrangement, we studied formation of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.032
更新日期：2004-12-01 00:00:00
abstract：:FbxA is a novel member of a family of proteins that contain an F-box and WD40 repeats and that target specific proteins for degradation via proteasomes. In fruiting bodies formed from cells where the fbxA gene is disrupted (fbxA(-) cells), the spore mass fails to fully ascend the stalk. In addition, fbxA(-) slugs cont...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9793
更新日期：2000-08-01 00:00:00
abstract：:Electroporation-based assays were used to test whether the myogenic regulatory factor (MRF) of Ciona intestinalis (CiMRF) interferes with endogenous developmental programs, and to evaluate the importance of its unusual N-terminus for muscle development. We found that CiMRF suppresses both notochord and endoderm develo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.10.010
更新日期：2019-04-15 00:00:00
abstract：:The universal signal for egg activation at fertilization is a rise in cytoplasmic Ca(2+) with defined spatial and temporal kinetics. Mammalian and amphibian eggs acquire the ability to produce such Ca(2+) signals during a maturation period that precedes fertilization and encompasses resumption of meiosis and progressi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.004
更新日期：2004-11-01 00:00:00
abstract：:We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca2+ release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.03.031
更新日期：2017-05-15 00:00:00
abstract：:A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprote...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.007
更新日期：2007-08-15 00:00:00
abstract：:We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90147-b
更新日期：1990-11-01 00:00:00
abstract：:The zebrafish T-box transcription factors spadetail (spt) and the brachyury ortholog no tail (ntl) are together essential for posterior mesoderm formation. In addition to being functionally redundant, spt and ntl also genetically interact with zygotic mutant alleles of one-eyed pinhead (Zoep), leading to synergistic m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.09.008
更新日期：2003-12-15 00:00:00
abstract：:Stem cells are essential for the regeneration and homeostasis of many organs, such as tooth, hair, skin, and intestine. Although human tooth regeneration is limited, a number of animals have evolved continuously growing teeth that provide models of stem cell-based organ renewal. A well-studied model is the mouse incis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.012
更新日期：2012-06-15 00:00:00
abstract：:Type 1 protein phosphatases (PP1) are involved in diverse cellular activities, ranging from glycogen metabolism to chromatin structure modification, mitosis, and meiosis. The holoenzymes are composed of two or more subunits, including a catalytic subunit (PP1c) and one or more regulatory subunits. Many eukaryotes poss...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9100
更新日期：1999-01-01 00:00:00
abstract：:We have isolated Xbr-1, a novel Xenopus homeobox gene that defines a new class of homeobox genes, distantly related to the Drosophila BarH1 and BarH2 genes. Xbr-1 is predominantly expressed in the developing Xenopus eye, starting as early as the neural plate stage. At early stages of eye development XBR-1 is expressed...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0055
更新日期：1996-02-25 00:00:00
abstract：:About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8972
更新日期：1998-09-01 00:00:00
abstract：:Bone morphogenetic protein (BMP) signalling is key to many developmental processes, including early regionalisation of the ectoderm. The neural crest is induced here by a combination of BMP and Wnt signals from nearby tissues with many secreted factors contributing to its initial specification at the neural plate bord...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.015
更新日期：2020-04-15 00:00:00
abstract：:Acute myeloid leukemia (AML) is a complex malignancy with poor prognosis. Several genetic lesions can lead to the disease. One of these corresponds to the NUP98-HOXA9 (NA9) translocation that fuses sequences encoding the N-terminal part of NUP98 to those encoding the DNA-binding domain of HOXA9. Despite several studie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.11.003
更新日期：2017-01-01 00:00:00
abstract：:Temporal and spatial aspects of floral determination in seedling terminal buds of the qualitative short-day plant Pharbitis nil were examined using a grafting assay. Floral determination in the terminal buds of 6-day-old P. nil seedlings is rapid; by 9 hr after the end of a 14-hr inductive dark period more than 50% of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90268-n
更新日期：1990-02-01 00:00:00
abstract：:Integrin α5-null embryos die in mid-gestation from severe defects in cardiovascular morphogenesis, which stem from defective development of the neural crest, heart and vasculature. To investigate the role of integrin α5β1 in cardiovascular development, we used the Mesp1(Cre) knock-in strain of mice to ablate integrin ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.014
更新日期：2014-11-15 00:00:00
abstract：:The insect head is composed of several segments. During embryonic development, the segments fuse to form a rigid head capsule where obvious segmental boundaries are lacking. Hence, the assignment of regions of the insect head to specific segments is hampered, especially with respect to dorsal (vertex) and lateral (gen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.11.010
更新日期：2010-02-01 00:00:00
abstract：:Somatic gonadal niche cells control the survival, differentiation, and proliferation of germline stem cells. The establishment of this niche-stem cell relationship is critical, and yet the precursors to these two cell types are often born at a distance from one another. The simple Caenorhabditis elegans gonadal primor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.03.019
更新日期：2013-07-15 00:00:00
abstract：:The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Gl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0127
更新日期：2001-03-01 00:00:00
abstract：:Hox genes are crucial players in the generation and pattering of the vertebrate trunk and posterior body during embryogenesis. Their initial expression takes place shortly after the establishment of the primitive streak, in the posterior-most part of the mouse embryo and is a determinant step for setting up the defini...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2017.02.009
更新日期：2017-08-15 00:00:00
abstract：:During limb development, Sonic hedgehog (SHH) and HOX proteins are considered among the most important factors regulating digit number and identity. SHH signaling prevents the processing of GLI3 into a short form that functions as a strong transcriptional repressor. Gli3 mutant limbs are characterized by a severe poly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.023
更新日期：2007-10-15 00:00:00
abstract：:Embryonic morphogenesis relies on the intrinsic ability of cells, often through remodeling the cytoskeleton, to shape epithelial tissues during development. Epithelial invagination is an example of morphogenesis that depends on this remodeling but the cellular mechanisms driving arrangement of cytoskeletal elements ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.02.014
更新日期：2020-06-01 00:00:00
abstract：:A mouse mammary epithelial cell line with morphogenetic properties in vivo, Comma-Dbeta, was used to isolate and to characterize mammary progenitor cells. We found that a homogeneous cell population expressing high surface levels of stem cell antigen 1 (Sca-1) was able to give rise in vivo to ductal and alveolar struc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.007
更新日期：2006-05-15 00:00:00
