Abstract:
:TGF-beta (TGFβ) family mediated Smad signaling is involved in mesoderm and endoderm specifications, left-right asymmetry formation and neural tube development. The TGFβ1/2/3 and Activin/Nodal signal transduction cascades culminate with activation of SMAD2 and/or SMAD3 transcription factors and their overactivation are involved in different pathologies with an inflammatory and/or uncontrolled cell proliferation basis, such as cancer and fibrosis. We have developed a transgenic zebrafish reporter line responsive to Smad3 activity. Through chemical, genetic and molecular approaches we have seen that this transgenic line consistently reproduces in vivo Smad3-mediated TGFβ signaling. Reporter fluorescence is activated in phospho-Smad3 positive cells and is responsive to both Smad3 isoforms, Smad3a and 3b. Moreover, Alk4 and Alk5 inhibitors strongly repress the reporter activity. In the CNS, Smad3 reporter activity is particularly high in the subpallium, tegumentum, cerebellar plate, medulla oblongata and the retina proliferative zone. In the spinal cord, the reporter is activated at the ventricular zone, where neuronal progenitor cells are located. Colocalization methods show in vivo that TGFβ signaling is particularly active in neuroD+ precursors. Using neuronal transgenic lines, we observed that TGFβ chemical inhibition leads to a decrease of differentiating cells and an increase of proliferation. Similarly, smad3a and 3b knock-down alter neural differentiation showing that both paralogues play a positive role in neural differentiation. EdU proliferation assay and pH3 staining confirmed that Smad3 is mainly active in post-mitotic, non-proliferating cells. In summary, we demonstrate that the Smad3 reporter line allows us to follow in vivo Smad3 transcriptional activity and that Smad3, by controlling neural differentiation, promotes the progenitor to precursor switch allowing neural progenitors to exit cell cycle and differentiate.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Casari A,Schiavone M,Facchinello N,Vettori A,Meyer D,Tiso N,Moro E,Argenton Fdoi
10.1016/j.ydbio.2014.09.025subject
Has Abstractpub_date
2014-12-01 00:00:00pages
81-93issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(14)00482-5journal_volume
396pub_type
杂志文章abstract::The distribution and ontogeny of four neuropeptides in developing chick lumbosacral sensory and sympathetic ganglia were studied using immunohistochemical techniques. Antibodies to two of these peptides, substance P (SP) and calcitonin gene-related peptide (CGRP), stained small neurons in the medial part of the dorsal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90137-5
更新日期:1986-08-01 00:00:00
abstract::Cyclin J is a cyclin family member that appears to have evolved before the metazoan radiation. Its evolutionary conservation argues for an important role but functional characterizations of Cyclin J have remained very limited. In Drosophila, Cyclin J is expressed only in females. Using transgenic Drosophila lines expr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.042
更新日期:2009-09-15 00:00:00
abstract::Single cells from 512-cell Xenopus embryos, totally labeled with intracellular horseradish peroxidase, were transplanted orthotopically (from either dorsal or lateral marginal zone) or heterotopically (between these two marginal zones) to unlabeled host embryos at the 512-cell stage. At tailbud stage 23 the locations,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80043-0
更新日期:1989-01-01 00:00:00
abstract::The iris sphincter muscle consists of striated muscle fibers in the adult chicken. The ontogenetic development of this muscle has been studied by immunocytochemistry, from Embryonic Day 7 to the time of hatching. The time course of expression of specific markers of either smooth or striated muscle, i.e., smooth and sk...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1254
更新日期:1993-10-01 00:00:00
abstract::The mechanisms involved in the formation and the differentiation of the liver remain unclear despite extensive studies. To investigate these events in mouse hepatic development, we have taken advantage of the N-myc mutant mouse line which exhibits abnormal liver development. N-myc mutant embryos die between 11.5 and 1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8823
更新日期:1998-03-01 00:00:00
abstract::Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to in...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.02.021
更新日期:2014-06-01 00:00:00
abstract::Initial studies suggested that spatial organization of the putative polar body contractile ring was determined by the peripheral aster in Spisula [Biol. Bull. 205 (2003) 192]. Here we report detailed supporting observations, including testing of aster and ring function with inhibitors. The metaphase peripheral aster w...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.01.033
更新日期:2004-05-15 00:00:00
abstract::Pax3 is a transcription factor expressed in somitic mesoderm, dorsal neural tube and pre-migratory neural crest during embryonic development. We have previously identified cis-acting enhancer elements within the proximal upstream genomic region of Pax3 that are sufficient to direct functional expression of Pax3 in neu...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.12.030
更新日期:2010-03-15 00:00:00
abstract::The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Gl...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.0127
更新日期:2001-03-01 00:00:00
abstract::In normal development the veg1 tier of the sixth cleavage Strongylocentrotus purpuratus embryo contributes progeny to both ectodermal lineages and portions of the archenteron. Treatment with 18 mM LiCl specifically affects this lineage allocation, reducing or eliminating the veg1 contribution to ectoderm. Less frequen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8615
更新日期:1997-07-15 00:00:00
abstract::Chondrogenesis of mesenchymal cells from the frontonasal mass, mandibles and maxillae of stage-24 chick embryos has been investigated in micromass (high-density) cultures. Distinct differences in the amount and pattern of cartilage differentiation are found. In cultures of frontonasal mass cells, a central sheet of ca...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90349-0
更新日期:1986-09-01 00:00:00
abstract::FbxA is a novel member of a family of proteins that contain an F-box and WD40 repeats and that target specific proteins for degradation via proteasomes. In fruiting bodies formed from cells where the fbxA gene is disrupted (fbxA(-) cells), the spore mass fails to fully ascend the stalk. In addition, fbxA(-) slugs cont...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9793
更新日期:2000-08-01 00:00:00
abstract::To examine whether the extracellular matrix (ECM) plays a role in mediating interactions between the apical ectodermal ridge (AER) and the subjacent mesoderm in the chick wing bud, we used immunohistochemistry to locate the following tissue components during wing morphogenesis: types I and IV collagens, fibronectin, t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1141
更新日期:1994-05-01 00:00:00
abstract::Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90023-0
更新日期:1986-12-01 00:00:00
abstract::The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1002
更新日期:1993-01-01 00:00:00
abstract::The evolutionary divergence of cnidarian and bilaterian lineages from their remote metazoan ancestor occurred at an unknown depth in time before the Cambrian, since crown group representatives of each are found in Lower Cambrian fossil assemblages. We report here a variety of putative embryonic, larval, and adult micr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0714
更新日期:2002-08-01 00:00:00
abstract::The nematode gonad is an exemplary system for the study of organogenesis and fundamental problems in developmental and cellular biology. Nematode gonads vary dramatically across species (Chitwood, B.G., Chitwood, M.B., 1950. Introduction to Nematology." University Park Press, Baltimore; Felix, M.A., Sternberg, P.W., 1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.09.021
更新日期:2005-01-01 00:00:00
abstract::We describe the identification, biochemical characterisation, and mutation of a novel mouse gene: Sp5. Sp5 encodes a protein having a C-terminal C(2)H(2) zinc finger domain closely related to that of the transcription factor Sp1. In vitro, DNA binding studies show that it binds to the GC box, a DNA motif present in th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9878
更新日期:2000-11-15 00:00:00
abstract::The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90441-6
更新日期:1987-09-01 00:00:00
abstract::The HCO3(-) anion activates sperm motility, an important early step in capacitation, by increasing flagellar beat frequency through a pathway that requires the atypical adenylyl cyclase SACY and the sperm-specific C alpha2 catalytic subunit of PKA. Here we show that the accelerating action of HCO3(-) also requires the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.09.017
更新日期:2007-12-01 00:00:00
abstract::These studies were designed to test the hypothesis that sperm nuclear decondensation and male pronuclear formation during hamster fertilization depend upon the ability of the fertilized oocyte to reduce sperm nuclear disulfide bonds. In a first series of experiments, treatment of mature oocytes with the sulfhydryl blo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90126-x
更新日期:1984-01-01 00:00:00
abstract::The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8915
更新日期:1998-07-01 00:00:00
abstract::Localisation of Protein Kinase A (PKA) by A-Kinase Anchoring Proteins (AKAPs) is known to coordinate localised signalling complexes that target cAMP-mediated signalling to specific cellular sub-domains. The cAMP PKA signalling pathway is implicated in both meiotic arrest and meiotic resumption, thus spatio-temporal ch...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.045
更新日期:2008-05-01 00:00:00
abstract::The Drosophila brain is generated by a complex series of morphogenetic movements. To better understand brain development and to provide a guide for experimental manipulation of brain progenitors, we created a fate map using photoactivated gene expression to mark cells originating within specific mitotic domains and ti...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00220-3
更新日期:2003-08-01 00:00:00
abstract::We have previously shown that the adenosine analog 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR), an activator of AMP-activated protein kinase (AMPK), stimulates an increase in AMPK activity and induces meiotic resumption in mouse oocytes [Downs, S.M., Hudson, E.R., Hardie, D.G., 2002. A potential rol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.039
更新日期:2006-03-15 00:00:00
abstract::A fusion gene construct containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of CyIIIa actin gene regulatory sequences was injected into unfertilized eggs of the urchin Strongylocentrotus purpuratus, and early pluteus stage embryos that developed from these eggs were fixed and sectio...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90193-x
更新日期:1987-06-01 00:00:00
abstract::A mutant gene dose-dependent inhibition of cerebellar granule cell neuron survival and neurite growth in dissociated cultures of cerebellum from 7-day-old heterozygous (+/wv) and homozygous (wv/wv) weaver mutant mice (M. Willinger, D. M. Margolis, and R. L. Sidman. (1981), J. Supramol. Struc. 17, 79-86) has previously...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90384-7
更新日期:1985-01-01 00:00:00
abstract::Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8592
更新日期:1997-06-15 00:00:00
abstract::We have investigated the ability of growing dictyate oocytes and early preimplantation embryos of the mouse to process extrachromosomal DNA molecules with free ends by intranuclearly microinjecting DNA fragments containing a region of homology of various extent at either the 5' or 3' terminus. Homologous recombination...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0199
更新日期:2001-05-01 00:00:00
abstract::The contribution of cranial neural crest cells to the development and patterning of cranial muscles in amphibians was investigated in the phylogenetically basal and morphologically generalized frog, Bombina orientalis. Experimental methods included fluorescent marking of premigratory cranial neural crest and extirpati...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0377
更新日期:2001-09-15 00:00:00