Abstract：
:Localisation of Protein Kinase A (PKA) by A-Kinase Anchoring Proteins (AKAPs) is known to coordinate localised signalling complexes that target cAMP-mediated signalling to specific cellular sub-domains. The cAMP PKA signalling pathway is implicated in both meiotic arrest and meiotic resumption, thus spatio-temporal changes in PKA localisation during development may determine the oocytes response to changes in cAMP. In this study we aim to establish whether changes in PKA localisation occur during oocyte and early embryo development. Using fluorescently-labelled PKA constructs we show that in meiotically incompetent oocytes PKA is distributed throughout the cytoplasm and shows no punctuate localisation. As meiotic competence is acquired, PKA associates with mitochondria. Immature germinal vesicle (GV) stage oocytes show an aggregation of PKA around the GV and PKA remains co-localised with mitochondria throughout oocyte maturation. After fertilisation, the punctuate, mitochondrial distribution was lost, such that by the 2-cell stage there was no evidence of PKA localisation. RT-PCR and Western blotting revealed two candidate AKAPs that are known to be targeted to mitochondria, AKAP1 and D-AKAP2. In summary these data show a dynamic regulation of PKA localisation during oocyte and early embryo development.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Webb RJ,Tinworth L,Thomas GM,Zaccolo M,Carroll Jdoi
10.1016/j.ydbio.2008.01.045subject
Has Abstractpub_date
2008-05-01 00:00:00pages
36-45issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(08)00097-3journal_volume
317pub_type
杂志文章abstract：:The canonical, beta-catenin-dependent Wnt pathway is a crucial player in the early events of Xenopus development. Dorsal axis formation and mesoderm patterning are accepted effects of this pathway, but the regulation of expression of genes involved in mesoderm specification is not. This conclusion is based largely on ...
journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
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pub_type： 杂志文章
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