Abstract：
:The HCO3(-) anion activates sperm motility, an important early step in capacitation, by increasing flagellar beat frequency through a pathway that requires the atypical adenylyl cyclase SACY and the sperm-specific C alpha2 catalytic subunit of PKA. Here we show that the accelerating action of HCO3(-) also requires the continued presence of external Ca2+ (EC50 approximately 0.5 mM), and find that Ca2+ can be replaced by Sr2+ but not by Mn2+. Ca2+ is required for HCO3(-) to elevate cAMP, but not for cAMP-AM to increase beat frequency, indicating that external Ca2+ acts before rather than after stimulation of SACY by HCO3(-). With external Ca2+ present, HCO3(-) does not alter cytosolic or near-membrane [Ca2+]. Removal of external Ca2+ initiates a slow decline in intracellular [Ca2+] and rapid block of the HCO3(-)-evoked acceleration that is not relieved upon increasing internal [Ca2+] by rapid photolysis of caged Ca2+. We also find that the rapid (t(1/2) approximately 10 s) accelerating action of HCO3(-) is slowed more than three-fold by the carbonic anhydrase inhibitor acetazolamide. It is unaltered by the broad spectrum anion transport inhibitor SITS, and is not accompanied by detectable changes in intracellular pH. We propose that external Ca2+ binds an unidentified extracellular protein that is required for HCO3(-) to engage cAMP-mediated activation of motility.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Carlson AE,Hille B,Babcock DFdoi
10.1016/j.ydbio.2007.09.017subject
Has Abstractpub_date
2007-12-01 00:00:00pages
183-92issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(07)01352-8journal_volume
312pub_type
杂志文章abstract：:In this study, we demonstrate the developmental activation, in the zebrafish embryo, of a surveillance mechanism which triggers apoptosis to remove damaged cells. We determine the time course of activation of this mechanism by exposing embryos to camptothecin, an agent which specifically inhibits topoisomerase I withi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9243
更新日期：1999-05-15 00:00:00
abstract：:BMP signaling responses are refined by distinct secreted and intracellular antagonists in different cellular and temporal contexts. Here, we show that the nuclear LEM-domain protein MAN1 is a tissue-specific antagonist of BMP signaling in Drosophila. MAN1 contains two potential Mad-binding sites. We generated MAN1Delt...
journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.031
更新日期：2005-08-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:Developing cardiac myocytes divide a limited number of times before they stop and terminally differentiate, but the mechanism that stops their division is unknown. To help study the stopping mechanism, we defined conditions under which embryonic rat cardiac myocytes cultured in serum-free medium proliferate and exit t...
journal_title：Developmental biology
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doi：10.1006/dbio.1999.9524
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.042
更新日期：2007-10-15 00:00:00
abstract：:Plants often display a high competence for regeneration under stress conditions. Signals produced in response to various types of stress serve as critical triggers for de novo organogenesis, but the identity of these signaling molecules underlying cellular reprogramming are largely unknown. We previously identified an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.006
更新日期：2018-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0440
更新日期：2001-12-01 00:00:00
abstract：:Hox genes are well-known master regulators in developmental morphogenesis along the anteroposterior axis of animals. However, the molecular mechanisms by which Hox proteins regulate their target genes and determine cell fates are not fully understood. The silk gland of Bombyx mori is a tubular tissue divided into seve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.002
更新日期：2014-02-01 00:00:00
abstract：:Developing vertebrate limbs are often utilized as a model for studying pattern formation and morphogenetic cell death. Herein, we report that conditional deletion of Rac1, a member of the Rho family of proteins, in mouse limb bud mesenchyme led to skeletal deformities in the autopod and soft tissue syndactyly, with th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.09.014
更新日期：2009-11-15 00:00:00
abstract：:The Drosophila homeobox gene tinman and its vertebrate homologs Nkx-2.5 and Nkx-2.3 are critical determinants of cardiac development. We report here the identification of a new tinman-related gene, nkx2.7, as well as orthologs of Nkx-2.5 and Nkx-2.3 in the zebrafish. Analysis of their expression in the developing zebr...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1996-12-15 00:00:00
abstract：:The notochord is the defining characteristic of the chordate embryo and plays critical roles as a signaling center and as the primitive skeleton. In this study we show that early notochord development in Xenopus embryos is regulated by apoptosis. We find apoptotic cells in the notochord beginning at the neural groove ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.08.047
更新日期：2007-11-15 00:00:00
abstract：:External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm,...
journal_title：Developmental biology
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doi：10.1006/dbio.2002.0668
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abstract：:Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.06.017
更新日期：2014-09-01 00:00:00
abstract：:To examine the possible role of homeo box genes in murine development we have studied the structure and expression pattern of Hox-2.5, a newly isolated homeo box gene that maps to the left end of the mouse Hox-2 locus on chromosome 11. The sequence of the Hox-2.5 homeo box has been determined. It is highly homologous ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90056-0
更新日期：1989-06-01 00:00:00
abstract：:Previous work in our laboratory has shown that a 62- to 64-kDa protein was a major component of the perinuclear ring of manchettes fractionated from rat spermatids. Mass spectrometry analysis of this protein indicated the presence of a glycine-rich domain homologous to human keratin 9 (K9). Several antibodies to K9, r...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2000-11-15 00:00:00
abstract：:We report the patterns of migration of Sertoli cells plated on specific substrata, and the influences of testicular peritubular cells on these processes. Data presented indicate that while peritubular cells readily spread when explanted onto Type I collagen, Sertoli cells do not. A delay of 4 to 6 days occurs after Se...
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90091-2
更新日期：1989-07-01 00:00:00
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journal_title：Developmental biology
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更新日期：2017-03-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1996-12-15 00:00:00
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journal_title：Developmental biology
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更新日期：2012-01-01 00:00:00
abstract：:An understanding of heart development is critical in any systems biology approach to cardiovascular disease. The interpretation of data generated from high-throughput technologies (such as microarray and proteomics) is also essential to this approach. However, characterizing the role of genes in the processes underlyi...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2011-06-01 00:00:00
abstract：:Recently, it has been suggested that a mouse sperm-associated protein with properties similar to the inhibitory guanine nucleotide-binding regulatory protein Gi plays an intermediary role in the zona pellucida-induced acrosome reaction (Endo, Y., Lee, M. A., and Kopf, G. S. 1987. Dev. Biol. 119, 210-216). In the prese...
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pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.07.016
更新日期：2003-12-01 00:00:00
abstract：:Stem cells are essential for the regeneration and homeostasis of many organs, such as tooth, hair, skin, and intestine. Although human tooth regeneration is limited, a number of animals have evolved continuously growing teeth that provide models of stem cell-based organ renewal. A well-studied model is the mouse incis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.03.012
更新日期：2012-06-15 00:00:00
abstract：:We have identified a cellular efflux pump, RhT, with the properties of an MDR transporter-a type of ATP-binding cassette transporter whose substrates include small hydrophobic molecules. RhT transports rhodamine 123 (Rh123) and is inhibited by low temperature, energy poisons, and several MDR transport inhibitors, such...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9611
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