Abstract:
:We have identified a cellular efflux pump, RhT, with the properties of an MDR transporter-a type of ATP-binding cassette transporter whose substrates include small hydrophobic molecules. RhT transports rhodamine 123 (Rh123) and is inhibited by low temperature, energy poisons, and several MDR transport inhibitors, such as verapamil. All vegetative cells have RhT activity, but during development prestalk cells lose RhT activity while prespore cells retain it. We also identified several RhT inhibitors. The most effective inhibitor is the stalk cell-inducing chlorinated alkyl phenone, DIF-1. The RhT inhibitors disrupted development, to varying degrees, and induced stalk cell formation in submerged culture. The inhibitors displayed the same rank order of pharmacological efficacy for stalk cell induction as they did for Rh123 transport inhibition. We also found that cerulenin, a specific inhibitor of DIF-1 biosynthesis (R. R. Kay, 1998, J. Biol. Chem. 273, 2669-2675), abolished the induction of stalk cells by each of the RhT inhibitors, and this effect could be reversed by DIF-1. Thus, DIF-1 synthesis appears to be required for the induction of stalk cells by the RhT inhibitors. Since DIF-1 is the most potent inhibitor of RhT activity, and thus a likely transport substrate itself, we propose that RhT inhibitors induce stalk cell differentiation by blocking DIF-1 export, causing DIF-1 to build up within cells. Our results provide evidence for a prespore-specific efflux pump that regulates cell fate determination, perhaps by regulating the cellular concentration of DIF-1.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Good JR,Kuspa Adoi
10.1006/dbio.2000.9611subject
Has Abstractpub_date
2000-04-01 00:00:00pages
53-61issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)99611-8journal_volume
220pub_type
杂志文章abstract::Expression of constitutively active Src protein tyrosine kinase in Xenopus oocytes has been shown to accelerate oocyte maturation suggesting that Src may be involved in meiotic progression. However, meiotic regulation of endogenous Src kinase in oocytes has not been investigated in detail. To address this problem, we ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.10.018
更新日期:2005-02-15 00:00:00
abstract::In Xenopus, the hatching and cement glands form at important boundaries in the head. We have examined induction of these glands in order to see the basis for this ectodermal patterning. Ectoderm can be induced to form hatching and cement glands by dorsal mesoderm and by the neural plate. Because the neural plate is su...
journal_title:Developmental biology
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pub_type: 杂志文章,评审
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2004-04-01 00:00:00
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1230
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pub_type: 杂志文章
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journal_title:Developmental biology
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pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8773
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journal_title:Developmental biology
pub_type: 杂志文章
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