Abstract：
:We have identified zebrafish orthologues of glial cell line-derived neurotrophic factor (GDNF) and the ligand-binding component of its receptor GFRalpha1. We examined the mRNA expression pattern of these genes in the developing spinal cord primary motor neurons (PMN), kidney, and enteric nervous systems (ENS) and have identified areas of correlated expression of the ligand and the receptor that suggest functional significance. Many aspects of zebrafish GDNF expression appear conserved with those reported in mouse, rat, and avian systems. In the zebrafish PMN, GFRalpha1 is only expressed in the CaP motor neuron while GDNF is expressed in the ventral somitic muscle that it innervates. To test the functional significance of this correlated expression pattern, we ectopically overexpressed GDNF in somitic muscle during the period of motor axon outgrowth and found specific perturbations in the pattern of CaP axon growth. We also depleted GDNF protein in zebrafish embryos using morpholino antisense oligos and found that GDNF protein is critical for the development of the zebrafish ENS but appears dispensable for the development of the kidney and PMN.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Shepherd IT,Beattie CE,Raible DWdoi
10.1006/dbio.2000.0145subject
Has Abstractpub_date
2001-03-15 00:00:00pages
420-35issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)90145-3journal_volume
231pub_type
杂志文章abstract：:In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.005
更新日期：2019-04-15 00:00:00
abstract：:The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8764
更新日期：1997-12-15 00:00:00
abstract：:We show that the intermediate filament protein nestin is expressed in myogenic cells and that multiple mechanisms regulate nestin expression at different stages of myogenesis. Cultured embryonic, fetal, and neonatal mouse limb myoblasts initially expressed nestin in the absence of the four muscle regulatory factors (M...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1248
更新日期：1994-09-01 00:00:00
abstract：:We investigated the influence of the growth surface on the direction of Xenopus spinal neurite growth in the presence of a dc electric field of physiological magnitude. The direction of galvanotropism was determined by the substratum; neurites grew toward the negative electrode (cathode) on untreated Falcon tissue cul...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9039
更新日期：1998-11-15 00:00:00
abstract：:Epi 1, a monoclonal antibody, was generated against an epidermal specific epithelial antigen; it does not stain neural epithelium. We have used Epi 1 as a marker to determine when the spatial patterns delineating neural from nonneural epithelium become established. We used ventral ectoderm in a sandwich assay to show ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90307-2
更新日期：1989-05-01 00:00:00
abstract：:Ephrin-B2, a member of the Eph/ephrin family of cell signaling molecules, has been implicated in the guidance of cranial and trunk neural crest cells (NCC) and development of the branchial arches(BA), but detailed examination in mice has been hindered by embryonic lethality of Efnb2 null loss of function due to a requ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.08.021
更新日期：2015-10-15 00:00:00
abstract：:The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1040
更新日期：1995-02-01 00:00:00
abstract：:All metazoan genomes encode multiple RAS GTPase activating proteins (RasGAPs) that negatively regulate the conserved RAS/MAPK signaling pathway. In mammals, several RasGAPs exhibit tumor suppressor activity by preventing excess RAS signal transduction. We have identified gap-3 as the to date missing Caenorhabditiseleg...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.08.026
更新日期：2008-11-15 00:00:00
abstract：:Specification of the primordial germ cells (PGCs) is essential for sexually reproducing animals. Although the mechanisms of PGC specification are diverse between organisms, the RNA binding protein Nanos is consistently required in the germ line in all species tested. How Nanos is selectively expressed in the germ line...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.04.010
更新日期：2019-08-01 00:00:00
abstract：:Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.02.021
更新日期：2014-06-01 00:00:00
abstract：:Embryonic development involves a series of cell adhesive interactions that provide mechanical and instructive information required for morphogenesis. The ADAMs family of membrane-anchored proteins, containing a disintegrin and metalloprotease domain, is well suited for participating in such developmental events. They ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8458
更新日期：1997-02-15 00:00:00
abstract：:Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9346
更新日期：1999-08-15 00:00:00
abstract：:Gastrulation in Xenopus laevis transforms a ball of undifferentiated cells into a well-organized elongated embryo with a distinct body axis, as a result of extensive cell rearrangements and movements. Since cell adhesion is thought to play an important role during tissue morphogenesis, we investigated the potential ro...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1288
更新日期：1995-10-01 00:00:00
abstract：:We have used amphibian gastrulation as a model system to study the action of the extracellular matrix (ECM) glycoprotein tenascin on mesodermal cell migration. Tenascin function was assayed in vitro during spreading of isolated cells from the dorsal marginal zone (DMZ) and during cell migration from DMZ explants. Plas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90256-i
更新日期：1990-02-01 00:00:00
abstract：:Exocytosis of cortical granules in mouse eggs is required to produce the zona pellucida block to polyspermy. In this study, we examined the role of microfilaments and microtubules in the regulation of cortical granule movement toward the cortex during oocyte maturation and anchoring of cortical granules in the cortex....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8945
更新日期：1998-08-01 00:00:00
abstract：:Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.08.009
更新日期：2016-10-01 00:00:00
abstract：:The levels of al-1 and al-2 transcripts change dramatically in response to light and development during the formation of Neurospora crassa asexual spores (conidia). al-1 and al-2 mRNAs accumulate throughout conidiation irrespective of lighting conditions initially at low levels. As conidiation proceeds, two increases ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1129
更新日期：1995-05-01 00:00:00
abstract：:We have identified cDNA clones encoding a testis-specific poly(A) polymerase, termed TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of preexisting mRNAs in male haploid germ cells. The TPAP gene was most abundantly expressed coincident with the additional elongation of mRNA poly(A) tails in rou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9894
更新日期：2000-12-01 00:00:00
abstract：:Previous experiments in which vitamin A has been administered to developing or regenerating limbs have shown that different limb axes are affected. In regenerating axolotl limbs, serial reduplications in the proximodistal axis are produced. In the developing chick limb bud, mirror-imaged reduplications in the anteropo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90370-6
更新日期：1983-08-01 00:00:00
abstract：:Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light) increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolariz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8600
更新日期：1997-07-01 00:00:00
abstract：:Islet1 (Isl1) is a LIM homedomain protein that plays a pivotal role in cardiac progenitors of the second heart field. Here, lineage studies with an inducible isl1-cre demonstrated that most Isl1 progenitors have migrated into the heart by E9. Although Isl1 expression is downregulated in most cardiac progenitors as the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.12.048
更新日期：2007-04-01 00:00:00
abstract：:The cardiac neural crest contains ectomesenchymal and neural anlagen that are necessary for normal heart development. It is not known whether other regions of the neural crest are capable of supporting normal heart development. In the experiments reported herein, quail donor embryos provided cardiac, trunk, or mesence...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90112-7
更新日期：1989-08-01 00:00:00
abstract：:FgfrL1, which interacts with Fgf ligands and heparin, is a member of the fibroblast growth factor receptor (Fgfr) family. FgfrL1-deficient mice show two significant alterations when compared to wildtype mice: They die at birth due to a malformed diaphragm and they lack metanephric kidneys. Utilizing gene arrays, qPCR ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.08.016
更新日期：2014-10-15 00:00:00
abstract：:Limb buds in the limbless chick begin to form normally but fail to form an AER and ultimately degenerate. Wnt7a and LMX-1, which are restricted to the dorsal half of a normal limb bud, are expressed throughout the ectoderm and mesenchyme of the mutant buds, respectively. Engrailed-1, normally expressed in ventral limb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0265
更新日期：1996-11-01 00:00:00
abstract：:A functional ribosomal protein mRNA, encoding the 60 S subunit protein L1, has been synthesized in vitro using bacteriophage SP6 RNA polymerase. This mRNA directs the synthesis of a product indistinguishable from L1 protein purified from Xenopus ovarian ribosomes. Our results show that L1 synthesis in stage VI oocytes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90247-3
更新日期：1988-03-01 00:00:00
abstract：:From July 19-24, 2015, 169 clinicians and basic scientists gathered in the vertiginous heights of Snowmass, Colorado (2502 m) for the fourth FASEB summer research conference on the 'Biology of Cilia and Flagella'. Organizers Maureen Barr (Rutgers University), Iain Drummond (Massachusetts General Hospital/Harvard Medic...
journal_title：Developmental biology
pub_type：
doi：10.1016/j.ydbio.2015.10.030
更新日期：2016-01-15 00:00:00
abstract：:The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.006
更新日期：2011-12-15 00:00:00
abstract：:We have cloned a Caenorhabditis elegans homologue of the Drosophila gap gene hunchback (hb) and have designated it hbl-1 (hunchback-like). hbl-1 encodes a predicted 982-amino-acid protein, containing two putative zinc-finger domains similar to those of Drosophila Hunchback. The gene is transcribed embryonically, but u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9096
更新日期：1999-01-15 00:00:00
abstract：:The Id1 and Id3 genes play major roles during cardiac development, despite their expression being confined to non-myocardial layers (endocardium-endothelium-epicardium). We previously described that Id1Id3 double knockout (dKO) mouse embryos die at mid-gestation from multiple cardiac defects, but early lethality precl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.10.004
更新日期：2011-01-01 00:00:00
abstract：:We have investigated the imprinting status of two insulin genes using an interspecific recombinant congenic mouse strain carrying Ins1 and Ins2 alleles from Mus spretus on a C57BL/6 genetic background. At Days 12.5, 13.5, and 14.5 of gestation, expression of both parental alleles of both Ins1 and Ins2 was detected in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1114
更新日期：1995-04-01 00:00:00
