Abstract：
:The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Glypican-3 (GPC3) deficiency was associated with abnormal and contrasting rates of proliferation and apoptosis in cortical (CCD) and medullary collecting duct (MCD) cells. In CCD, cell proliferation was increased threefold. In MCD, apoptosis was increased 16-fold. Expression of Gpc3 mRNA in ureteric bud and collecting duct cells suggested that GPC3 can exert direct effects in these cells. Indeed, GPC3 deficiency abrogated the inhibitory activity of BMP2 on branch formation in embryonic kidney explants, converted BMP7-dependent inhibition to stimulation, and enhanced the stimulatory effects of KGF. Similar comparative differences were found in collecting duct cell lines derived from GPC3-deficient and wild type mice and induced to form tubular progenitors in vitro, suggesting that GPC3 directly controls collecting duct cell responses. We propose that GPC3 modulates the actions of stimulatory and inhibitory growth factors during branching morphogenesis.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Grisaru S,Cano-Gauci D,Tee J,Filmus J,Rosenblum NDdoi
10.1006/dbio.2000.0127subject
Has Abstractpub_date
2001-03-01 00:00:00pages
31-46issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)90127-1journal_volume
231pub_type
杂志文章abstract：:Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8592
更新日期：1997-06-15 00:00:00
abstract：:Initiation of the development of the anterior-posterior axis in the mouse embryo has been thought to take place only when the anterior visceral endoderm (AVE) emerges and starts its asymmetric migration. However, expression of Lefty1, a marker of the AVE, was recently found to initiate before embryo implantation. This...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.020
更新日期：2007-09-01 00:00:00
abstract：:teashirt (tsh) encodes a zinc-finger protein that is thought to be part of a network that contributes to regionalization of the Drosophila embryo and establishes the domains of Hox protein function. tsh and the Hox gene Sex combs reduced (Scr) are essential to establish the identity of the first thoracic segment. We u...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.04.028
更新日期：2007-07-01 00:00:00
abstract：:To determine whether low oxygen is a stimulus for endothelial cell differentiation and vascular development in the kidney, we examined the effect of low oxygen on rat metanephric organ culture, a model known to recapitulate nephrogenesis in the absence of vessels. After 6 days in culture in standard (20% O2) or low ox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8513
更新日期：1997-03-15 00:00:00
abstract：:Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.009
更新日期：2007-02-15 00:00:00
abstract：:Aurora-C was first identified during screening for kinases expressed in mouse sperm and eggs. Herein, we report for the first time the precise subcellular localization of endogenous Aurora-C during male meiotic division. The localization of Aurora-C was analyzed by immunofluorescence staining on chromosome spreads of ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.11.036
更新日期：2006-02-15 00:00:00
abstract：:Temporal and spatial aspects of floral determination in seedling terminal buds of the qualitative short-day plant Pharbitis nil were examined using a grafting assay. Floral determination in the terminal buds of 6-day-old P. nil seedlings is rapid; by 9 hr after the end of a 14-hr inductive dark period more than 50% of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90268-n
更新日期：1990-02-01 00:00:00
abstract：:Metathoracic limb buds were removed unilaterally from Locusta migratoria embryos at 30% of embryonic development, thereby depriving limb-innervating neurons of the opportunity of innervating their normal target muscles. The operated embryos were allowed to hatch and develop to adulthood, and then the connections betwe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90335-5
更新日期：1985-02-01 00:00:00
abstract：:The arterial pole of the heart is the region where the ventricular myocardium continues as the vascular smooth muscle tunics of the aorta and pulmonary trunk. It has been shown that the arterial pole myocardium derives from the secondary heart field and the smooth muscle tunic of the aorta and pulmonary trunk derives ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.02.012
更新日期：2005-05-01 00:00:00
abstract：:CD9 is a membrane protein belonging to the tetraspanin family. Despite CD9's broad tissue distribution, the only abnormality observed in CD9-deficient mice was infertility of females, which was responsible for a defect in the sperm-egg fusion process. However, the function of CD9 in sperm-egg fusion is not clear at al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0694
更新日期：2002-07-15 00:00:00
abstract：:The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90241-7
更新日期：1987-04-01 00:00:00
abstract：:c-fms and c-kit are structurally related tyrosine kinase receptors for colony-stimulating factor-1 (CSF-1) and for steel factor (SF), respectively. The level of c-fms mRNA, like c-kit mRNA, increases during the maturation of oocytes and is an abundant maternal message found in the unfertilized oocyte. Following fertil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90207-w
更新日期：1992-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.009
更新日期：2006-11-15 00:00:00
abstract：:The salivary system in mammals is comprised of three independently developed pairs of organs, the parotid, submaxillar, and sublingual glands. Each gland is composed of various ductal and acinar cell types that fulfill multiple roles. However, the molecular mechanisms regulating their biogenesis and functions are stil...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0473
更新日期：2001-12-15 00:00:00
abstract：:How natural variation in embryo size affects patterning of the Drosophila embryo dorsal-ventral (DV) axis is not known. Here we examined quantitatively the relationship between nuclear distribution of the Dorsal transcription factor, boundary positions for several target genes, and DV axis length. Data were obtained f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.020
更新日期：2013-09-01 00:00:00
abstract：:Vertebrate morphology has been evolutionarily modified by natural and/or artificial selection. The morphological variation of goldfish is a representative example. In particular, the twin-tail strain of ornamental goldfish shows highly diverged anal and caudal fin morphology: bifurcated anal and caudal fins. Recent mo...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.11.022
更新日期：2017-07-15 00:00:00
abstract：:The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell-cell adhesion is known to play a crucial role in the directi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.02.007
更新日期：2016-03-15 00:00:00
abstract：:Activation of progenitor cells is crucial to promote tissue repair following injury in adult animals. In the context of successful limb regeneration following amputation, progenitor cells residing within the stump must re-enter the cell cycle to promote regrowth of the missing limb. We demonstrate that in axolotls, am...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.07.010
更新日期：2018-01-15 00:00:00
abstract：:The basic helix-loop-helix transcription factor Twist1 plays an essential role in mesenchymal cell populations during embryonic development and in pathological disease. Remodeling of the cardiac outflow tract (OFT) into the functionally separate aortic arch and pulmonary trunk is dependent upon the dynamic, coordinate...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.037
更新日期：2008-08-01 00:00:00
abstract：:Global silencing of transcriptional activity in the oocyte genome occurs just before the resumption of meiosis and is a crucial developmental transition at the culmination of oogenesis. Transcriptionally quiescent oocytes rely on stored maternal transcripts to sustain the completion of meiosis, fertilization, and earl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.028
更新日期：2004-11-15 00:00:00
abstract：:The protein tyrosine phosphatase PTP1, which mediates reversible phosphorylation on tyrosine, has been shown to play an important regulatory role during Dictyostelium development. Mutants lacking PTP1 develop more rapidly than normal, while strains that overexpress PTP1 display aberrant morphology. However, the signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0171
更新日期：2001-04-01 00:00:00
abstract：:The Hedgehog (Hh) signal transduction pathway plays a central role in the development of invertebrates and vertebrates. While much is known about the pathway, the role of Suppressor of fused (Su(fu)), a component of the pathway's signaling complex has remained enigmatic. Previous studies have linked Su(fu) to the cyto...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.050
更新日期：2006-06-01 00:00:00
abstract：:Caenorhabditis elegans PEB-1 is a novel DNA-binding protein expressed in most pharyngeal cell types and outside the pharynx in the hypodermis, hindgut, and vulva. Previous RNAi analyses indicated that PEB-1 is required for normal morphology of these tissues and growth; however, the peb-1 null phenotype was unknown. He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.08.040
更新日期：2004-12-15 00:00:00
abstract：:The acquisition of specialized skeletal muscle fiber phenotypes during development is investigated by systematic measurement of the accumulation of 21 contractile protein mRNAs during hindlimb development in the rat and the human. During early myotube formation in both species there is no coordination of expression of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90457-e
更新日期：1991-07-01 00:00:00
abstract：:The surface of eggs of the ascidian Halocynthia roretzi, observed with SEM, is essentially smooth until immediately before cell division when numerous microvilli appear and remain during cytokinesis. As the dividing blastomeres become closely adherent, however, the microvilli disappear and the eggs recover their smoot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90214-8
更新日期：1984-04-01 00:00:00
abstract：:cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90268-0
更新日期：1988-07-01 00:00:00
abstract：:We have examined the interfaces between protein and lipid sulfation, the response of progenitor cells to specific growth factors, and developmental lineage progression. The experimental system used is the oligodendrocyte (OL), the cell responsible for myelination of axons in the central nervous system. The ability to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1105
更新日期：1994-04-01 00:00:00
abstract：:Hippo (Hpo) signaling plays a critical role in restricting tissue growth and organ size in both invertebrate and vertebrate animals. However, how the Hpo kinase is regulated during development has not been clearly understood. Using a Bimolecular Fluorescence Complementation assay, we have investigated the functional s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:We have isolated two related Xenopus homologues of the homeotic zinc finger protein Teashirt1 (Tsh1), XTsh1a and XTsh1b. While Drosophila teashirt specifies trunk identity in the fly, the developmental relevance of vertebrate Tsh homologues is unknown. XTsh1a/b are expressed in prospective trunk CNS throughout early n...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.041
更新日期：2006-10-01 00:00:00
abstract：:The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90090-4
更新日期：1986-11-01 00:00:00
