Abstract：
:The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization. It is not clear, however, whether the nature of the sperm dysfunction is the same in all t haplotypes. Studies to date have focused on only one or two aspects of sperm function or have not examined sperm from males carrying different t haplotypes. In addition, factors within the t haplotypes interact with the genetic background, so that comparisons to sperm from t/+ or +/+ mice having different alleles at loci outside the t haplotypes may not be valid. To determine the nature of the sperm dysfunction caused by the t haplotypes, we have studied sperm from mice of the same genetic strain carrying none, one, or two t haplotypes. Sperm from tw32/+, tw5/+, and tw32/tw5 mice exhibited premature hyperactivation, a type of vigorous but nonprogressive motility correlated with fertility, while their rates of capacitation (the ability to undergo the acrosome reaction in response to zona proteins) and spontaneous acrosome reaction were similar to those of wild-type sperm. In addition, sperm flagellar curvature was abnormal: flagella from heterozygotes had an acute bend in the midpiece, giving the sperm a "fishhook" appearance, while the entire flagellum of sperm from tw32/tw5 mice was curled. Also, fewer sperm from tw32/tw5 mice were initially motile. Since all of these motility defects were dependent on exogenous calcium, the t haplotypes could specify an abnormal, calcium-sensitive component of the flagellum. The motility defects could also contribute to the dysfunction of these sperm by inhibiting their passage to the site of fertilization in vivo.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Olds-Clarke P,Johnson LRdoi
10.1006/dbio.1993.1002subject
Has Abstractpub_date
1993-01-01 00:00:00pages
14-25issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(83)71002-Xjournal_volume
155pub_type
杂志文章abstract：:The smaller AB-cell of the 2-cell embryo of Tubifex divides 40 min later than the larger CD-cell. The present study was undertaken to elucidate those factors that are responsible for this cleavage asynchrony. An examination of chromosome cycles showed that the AB-cell entered M-phase 5 min later and spent about 35 min...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1123
更新日期：1993-05-01 00:00:00
abstract：:We have characterized the antigen recognized by mab10, a monoclonal antibody that has been shown to modify outgrowth of thalamic and cortical axons in vitro, and investigated the influence of this antibody on axonal growth in the chicken retina in vivo. Immunopurification, peptide sequencing, and biochemical character...
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1987-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1991-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2015-06-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1115
更新日期：1994-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2003-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1984-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0184
更新日期：1996-08-01 00:00:00
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journal_title：Developmental biology
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更新日期：1999-11-01 00:00:00
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journal_title：Developmental biology
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更新日期：2010-10-01 00:00:00
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journal_title：Developmental biology
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abstract：:The salivary system in mammals is comprised of three independently developed pairs of organs, the parotid, submaxillar, and sublingual glands. Each gland is composed of various ductal and acinar cell types that fulfill multiple roles. However, the molecular mechanisms regulating their biogenesis and functions are stil...
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