Abstract：
:Sonic hedgehog is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb. The signaling pathway mediating the transduction of the signal is still poorly understood. In Drosophila several genes are known to act downstream of hedgehog, the fly homolog of Sonic hedgehog. An important gene epistatic to hedgehog is cubitus interruptus, which encodes the fly homolog of a family of vertebrate putative transcription factors, the GLI genes. We have isolated two members of the GLI family from chick, called GLI and GLI3. Their expression patterns in a variety of tissues during embryogenesis suggest that these genes may be targets of the Sonic hedgehog signal. We demonstrate that the two GLI genes are differentially regulated by Sonic hedgehog during limb development. Sonic hedgehog up-regulates GLI transcription, while down-regulating GLI3 expression in the mesenchymal cells of the developing limb bud. Finally, we demonstrate that an activated form of GLI can induce expression of Patched, a known target of Sonic hedgehog, thus implicating GLI as a key transcription factor in the vertebrate hedgehog signaling pathway. In conjunction with evidence from a mouse Gli3 mutant, our data suggest that GLI and GLI3 may have taken two different functions of their Drosophila homolog cubitus interruptus.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Marigo V,Johnson RL,Vortkamp A,Tabin CJdoi
10.1006/dbio.1996.0300subject
Has Abstractpub_date
1996-11-25 00:00:00pages
273-83issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90300-0journal_volume
180pub_type
杂志文章abstract：:Synapse elimination was examined in the developing frog cutaneous pectoris muscle using histological and electrophysiological techniques. Morphological synapse elimination occurred in two phases. The first phase, which began at the time of metamorphosis and continued until the second to third postmetamorphic week, was...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90279-8
更新日期：1983-10-01 00:00:00
abstract：:To determine whether low oxygen is a stimulus for endothelial cell differentiation and vascular development in the kidney, we examined the effect of low oxygen on rat metanephric organ culture, a model known to recapitulate nephrogenesis in the absence of vessels. After 6 days in culture in standard (20% O2) or low ox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8513
更新日期：1997-03-15 00:00:00
abstract：:Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.04.017
更新日期：2019-08-15 00:00:00
abstract：:An immunocytochemical study of cholecalcin (28-kDa calcium-binding protein, CaBP, calbindin) was carried out during the development of the rat hippocampus. In normal animals, the protein appeared from Postnatal Day 3 in the granule cells of the dentate gyrus and from Day 5 in the CA1-CA2 pyramidal cells of Ammon's hor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90474-x
更新日期：1987-11-01 00:00:00
abstract：:Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.01.010
更新日期：2017-02-15 00:00:00
abstract：:We have used an in vitro model to investigate the relationship between cell proliferation, cell maturation, and intercellular junctional changes that are seen during normal liver development. Chick liver fragments of embryonic stages 21, 28, and 39 were grown in organ culture in Medium 199 with 10% bovine serum albumi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90265-8
更新日期：1983-09-01 00:00:00
abstract：:Several genomic clones were isolated from a Drosophila library screened with cDNA prepared from abundant adult female mRNA. Cytoplasmic dot hybridizations have shown that the genes in all of these clones are expressed only in posteclosion (stages 8-14) follicles. One set of overlapping clones (lambda 20, lambda 28, an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90271-9
更新日期：1984-09-01 00:00:00
abstract：:Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90389-1
更新日期：1986-03-01 00:00:00
abstract：:Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct regions, exhibiting differences in m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.06.004
更新日期：2018-09-15 00:00:00
abstract：:Wnt-4, a member of the Wnt family of secreted signaling molecules, is essential for nephrogenesis, but its expression in the presumptive medulla suggests additional developmental roles in kidney organogenesis. We demonstrate here that Wnt-4 signaling plays also a role in the determination of the fate of smooth muscle ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.019
更新日期：2006-05-15 00:00:00
abstract：:Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.032
更新日期：2005-08-15 00:00:00
abstract：:The oblique muscle layer in the leech body wall is built upon the processes of a unique identified embryonic cell, the Comb- or C-cell. Each C-cell is composed of a spindle-shaped soma that projects approximately 70 parallel processes through the developing body wall at an angle oblique to the long axis. The morphogen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90342-z
更新日期：1991-11-01 00:00:00
abstract：:Syndecan, a cell surface proteoglycan, binds multiple extracellular ligands, and is developmentally regulated in epithelial and mesenchymal tissues. The branching morphogenesis of embryonic lung is dependent on epithelial-mesenchymal interactions and, based on studies with inhibitors, on proteoglycan synthesis. To ass...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90286-c
更新日期：1991-10-01 00:00:00
abstract：:Retinal stem cells (RSCs) exist as rare pigmented ciliary epithelial cells in adult mammalian eyes. We hypothesized that RSCs are at the top of the retinal cell lineage. Thus, genes expressed early in embryonic development to establish the retinal field in forebrain neuroectoderm may play important roles in RSCs. Pax6...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.021
更新日期：2007-04-15 00:00:00
abstract：:Sprouty proteins function as negative regulators of the receptor tyrosine kinase (RTK)-mediated Ras/Raf/MAPK pathway in many varied physiological and developmental processes, inhibiting growth factor-induced cellular proliferation, migration and differentiation. Like other negative regulators, Sprouty proteins are exp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.09.005
更新日期：2015-10-15 00:00:00
abstract：:The epidermis forms a vital barrier composed of stratified keratinocytes and their differentiated products. One of these products, keratin K10, is critical to epidermal integrity, because mutations in k10 lead to abnormal blistering. For the normal expression of k10, differentiation-associated transcription factors C/...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9460
更新日期：1999-12-01 00:00:00
abstract：:Developmental boundaries ensure that cells fated to participate in a particular structure are brought together or maintained at the appropriate locale within developing embryos. Parasegment grooves mark the position of boundaries that separate every segment of the Drosophila embryo into anterior and posterior compartm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.026
更新日期：2008-09-15 00:00:00
abstract：:We have isolated a nonneuronal cell line from Xenopus retinal neuroepithelium (XR1 cell line). On the basis of immunocytochemical characterization using monoclonal antibodies generated in our laboratory as well as several other glial-specific antibodies, we have established that the XR1 cells are derived from embryoni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90086-9
更新日期：1989-07-01 00:00:00
abstract：:Vascular smooth muscle of the head derives from neural crest, but developmental mechanisms and early transcriptional drivers of the vSMC lineage are not well characterized. We find that in early development, the transcription factor foxc1b is expressed in mesenchymal cells that associate with the vascular endothelium....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.005
更新日期：2019-09-01 00:00:00
abstract：:The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present st...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90188-7
更新日期：1989-10-01 00:00:00
abstract：:It is known that secreted proteins from the anterior lateral endoderm, FGF8 and BMP2, are involved in mesodermal cardiac differentiation, which determines the first cardiac field, defined by the expression of the earliest specific cardiac markers Nkx-2.5 and Gata4. However, the molecular mechanisms responsible for ear...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.07.007
更新日期：2015-10-01 00:00:00
abstract：:The zebrafish hedgehog (hh) family members tiggy-winkle hedgehog (twhh) and sonic hedgehog (shh) are involved in patterning the ventral CNS and proximal eye. Using a dominant negative protein kinase A regulatory subunit mutant, we show that these hh activities are mediated by protein kinase A. The effects of dominant ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0209
更新日期：1996-08-25 00:00:00
abstract：:Formation of the adenohypophysis in mammalian embryos occurs via an invagination of the oral ectoderm to form Rathke's pouch, which becomes exposed to opposing dorsoventral gradients of signaling proteins governing specification of the different hormone-producing pituitary cell types. One signal promoting pituitary ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00124-0
更新日期：2003-02-01 00:00:00
abstract：:Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.006
更新日期：2020-04-15 00:00:00
abstract：:Retinoic acid (RA) plays a critical role in neural patterning and organogenesis in the vertebrate embryo. Here we characterize a mutant of the zebrafish named giraffe (gir) in which the gene for the RA-degrading enzyme Cyp26a1 is mutated. The gir mutant displayed patterning defects in multiple organs including the com...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.023
更新日期：2005-02-15 00:00:00
abstract：:Extracellular modulators of cell-cell signaling control numerous aspects of organismal development. The Twisted gastrulation (Twsg1) gene product is a small, secreted cysteine-rich protein that has the unusual property of being able to either enhance or inhibit signaling by the bone morphogenetic protein (BMP) subfami...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.11.015
更新日期：2004-03-15 00:00:00
abstract：:To analyze the molecular mechanism of the limb pattern formation, we have tried to make monoclonal antibodies against antigens from chick limb buds. We obtained one antibody named AV-1 which recognized a specific region of chick limb buds. AV-1 reacted with the distal portion of the anteroventral mesoderm of only deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90337-4
更新日期：1986-10-01 00:00:00
abstract：:The gastrointestinal (GI) tract of metazoans is lined by a series of regionally distinct epithelia. To maintain structure and function of the GI tract, regionally diversified differentiation of somatic stem cell (SC) lineages is critical. The adult Drosophila midgut provides an accessible model to study SC regulation ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.08.027
更新日期：2016-11-15 00:00:00
abstract：:The unfertilized oocyte contains various granules which serve as storage sites for proteins, the majority which are yolk granules or platelets. Yolk granules are thought to provide the developing embryo with nutrients essential for its survival, while other granules contain proteins such as enzymes and extracellular m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1040
更新日期：1995-02-01 00:00:00
abstract：:Bone morphogenetic protein-4 (BMP-4) induces epidermis and represses neural fate in Xenopus ectoderm. Our previous findings implicate p42 Erk MAP kinase (MAPK) in the response to neural induction. We have examined the effects of BMP-4 on MAPK activity in gastrula ectoderm. Expression of a dominant negative BMP-4 recep...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0338
更新日期：2001-08-15 00:00:00
