Abstract：
:We have isolated a nonneuronal cell line from Xenopus retinal neuroepithelium (XR1 cell line). On the basis of immunocytochemical characterization using monoclonal antibodies generated in our laboratory as well as several other glial-specific antibodies, we have established that the XR1 cells are derived from embryonic astroglia. A monolayer of XR1 cells serves as an excellent substrate upon which embryonic retinal explants attach and elaborate neurites. This neurite outgrowth promoting activity appears not to be secreted into the medium, as medium conditioned by XR1 cells is ineffective in promoting outgrowth. Cell-free substrates were prepared to examine whether outgrowth promoting activity is also associated with the XR1 extracellular matrix (ECM). Substrates derived from XR1 cells grown on collagen are still capable of promoting outgrowth following osmotic shock and chemical extraction. This activity does not appear to be associated with laminin or fibronectin. Scanning electron microscopy was used to examine growth cones of retinal axons on XR1 cells and other substrates that supported neurite outgrowth. Growth cones and neurites growing on a monolayer of XR1 cells, or on collagen conditioned by XR1 cells, closely resemble the growth cones of retinal ganglion cells in vivo. A polyclonal antiserum (NOB1) generated against XR1 cells effectively and specifically inhibits neurite outgrowth on XR1-conditioned collagen. We therefore propose that neurite outgrowth promoting factors produced by these cells are associated with the extracellular matrix and may be glial specific.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Sakaguchi DS,Moeller JF,Coffman CR,Gallenson N,Harris WAdoi
10.1016/0012-1606(89)90086-9subject
Has Abstractpub_date
1989-07-01 00:00:00pages
158-74issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(89)90086-9journal_volume
134pub_type
杂志文章abstract：:The Drosophila homeobox gene tinman and its vertebrate homologs Nkx-2.5 and Nkx-2.3 are critical determinants of cardiac development. We report here the identification of a new tinman-related gene, nkx2.7, as well as orthologs of Nkx-2.5 and Nkx-2.3 in the zebrafish. Analysis of their expression in the developing zebr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0341
更新日期：1996-12-15 00:00:00
abstract：:Patterning of the early neural tube is achieved in part by the inductive signals, which arise from neuroepithelial signaling centers. The zona limitans intrathalamica (ZLI) is a neuroepithelial domain in the alar plate of the diencephalon which separates the prethalamus from the thalamus. The ZLI has recently been con...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.05.031
更新日期：2005-08-15 00:00:00
abstract：:Selected stages of the developing chicken cornea have been examined for type VI collagen, employing monoclonal antibodies specific for this molecule. By immunofluorescence, the molecule is not detectable in 5 1/2 day corneas, a time at which the epithelial-derived, acellular primary stroma is the only corneal matrix p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90013-8
更新日期：1986-12-01 00:00:00
abstract：:Vertebrate voluntary muscles are composed of myotubes and connective tissue cells. These two cell types have different embryonic origins: myogenic cells arise from paraxial mesoderm, while in the head many of the connective tissues are formed by neural crest cells. The objective of this research was to study interacti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90138-7
更新日期：1986-08-01 00:00:00
abstract：:Caenorhabditis elegans embryonic elongation depends on both epidermal and muscle cells. The hemidesmosome-like junctions, commonly called fibrous organelles (FOs), that attach the epidermis to the extracellular matrix ensure muscle anchoring to the cuticular exoskeleton and play an essential role during elongation. To...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.11.025
更新日期：2011-02-15 00:00:00
abstract：:Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90170-2
更新日期：1984-03-01 00:00:00
abstract：:Previous attempts to study sorting out of Drosophila imaginal disc cells have been hampered by an inability to thoroughly dissociate these cells and the need to use cuticular markers which require several days of in vivo culture. This study overcomes these limitations by using a new dissociation procedure and a geneti...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90049-1
更新日期：1985-04-01 00:00:00
abstract：:Mutations at the Drosophila melanogaster locus female sterile (1) homeotic (fs(1)h) result in segmental abnormalities including missing organs and homeotic transformations in the progeny of mutant mothers. Homeotic transformations are enhanced when the zygotes carry one of several third chromosome mutations, specifica...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90392-1
更新日期：1986-03-01 00:00:00
abstract：:The cranial base is a component of the neurocranium and has a central role in the structural integration of the face, brain and vertebral column. Consequently, alteration in the shape of the human cranial base has been intimately linked with primate evolution and defective development is associated with numerous human...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.12.024
更新日期：2016-07-15 00:00:00
abstract：:Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.07.014
更新日期：2019-11-15 00:00:00
abstract：:It is well established that the notochord influences the development of adjacent neural and mesodermal tissue. Involvement of the notochord in the differentiation of the dorsal pancreas has been demonstrated. However, our knowledge of the signals involved in pancreatic development is still incomplete. In order to iden...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9348
更新日期：1999-08-15 00:00:00
abstract：:We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells wi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0537
更新日期：2002-02-15 00:00:00
abstract：:The mature heart valves and septa are derived from the cardiac cushions which initially form as local outgrowths of mesenchymal cells within the outflow tract and atrioventricular regions. Endocardial cells respond to signals from the overlying myocardium and undergo an epithelial-to-mesenchymal transformation to inva...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0284
更新日期：2001-07-15 00:00:00
abstract：:We report periodic movements during erection of Dictyostelium discoideum (Dd) sorocarps. Our observations lead to the working hypothesis that Dd sorocarp erection occurs by two superimposed processes: one periodic, with a modal period of 6 1/2 min, and one continuous. We tentatively identify the periodic process with ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(76)90237-2
更新日期：1976-09-01 00:00:00
abstract：:Immunological approaches were used to characterize an antigen that is present within the cortical granules of mouse oocytes and eggs. Immunoelectron microscopy shows a specific localization of the antigen to the cortical granules in the cortex of mouse oocytes and eggs. Following in vitro fertilization, the antigen is...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90392-v
更新日期：1990-10-01 00:00:00
abstract：:The dopaminergic neurons of the basal ganglia play critical roles in CNS function and human disease, but specification of dopamine neuron phenotype is poorly understood in vertebrates. We performed an in vivo screen in zebrafish to identify dopaminergic neuron enhancers, in order to facilitate studies on the specifica...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.01.023
更新日期：2011-04-15 00:00:00
abstract：:During development of the central nervous system (CNS), commissural axons grow toward the ventral midline. After crossing the floor plate, they abruptly change their trajectory from the circumferential to the longitudinal axis. The contacts between the commissural axons and the floor plate cells are involved in this a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.05.034
更新日期：2004-09-15 00:00:00
abstract：:Although local epithelial-mesenchymal tissue interactions which are presumably mediated by extracellular matrix molecules are important regulators of tooth morphogenesis and differentiation, our studies have indicated that these developmental processes also depend on circulating molecules. The iron-carrying serum prot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90437-3
更新日期：1985-09-01 00:00:00
abstract：:To examine whether the extracellular matrix (ECM) plays a role in mediating interactions between the apical ectodermal ridge (AER) and the subjacent mesoderm in the chick wing bud, we used immunohistochemistry to locate the following tissue components during wing morphogenesis: types I and IV collagens, fibronectin, t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1141
更新日期：1994-05-01 00:00:00
abstract：:Formation of the adenohypophysis in mammalian embryos occurs via an invagination of the oral ectoderm to form Rathke's pouch, which becomes exposed to opposing dorsoventral gradients of signaling proteins governing specification of the different hormone-producing pituitary cell types. One signal promoting pituitary ce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00124-0
更新日期：2003-02-01 00:00:00
abstract：:Fertilized eggs of the leech Helobdella triserialis undergo a cytoplasmic reorganization which generates domains of nonyolky cytoplasm, called teloplasm, at the animal and vegetal poles. The segregation of teloplasm to one cell of the eight-cell embryo is responsible for a unique developmental fate of that cell, i.e.,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90181-4
更新日期：1989-10-01 00:00:00
abstract：:Although the formation and maintenance of epithelial tubes are essential for the viability of multicellular organisms, our understanding of the molecular and cellular events coordinating tubulogenesis is relatively limited. Here, we focus on the activities of Ribbon, a novel BTB-domain containing nuclear protein, in t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.05.541
更新日期：2008-08-01 00:00:00
abstract：:Proliferating intestinal stem cells (ISCs) generate all cell types of the Drosophila midgut, including enterocytes, endocrine cells, and gland cells (e.g., copper cells), throughout the lifetime of the animal. Among the signaling mechanisms controlling the balance between ISC self-renewal and the production of differe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.018
更新日期：2016-08-15 00:00:00
abstract：:Individual somatic muscles and heart progenitors are specified at defined positions within the mesodermal layer of Drosophila. The expression of the homeobox gene even-skipped (eve) identifies one specific subset of cells in the dorsal mesoderm, which give rise to particular pericardial cells and dorsal body wall musc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0397
更新日期：2001-10-01 00:00:00
abstract：:Gastrulation is the first major morphogenetic movement in development and requires dynamic regulation of cell adhesion and the cytoskeleton. Caenorhabditis elegans gastrulation begins with the migration of the two endodermal precursors, Ea and Ep, from the surface of the embryo into the interior. Ea/Ep migration provi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.507
更新日期：2010-08-15 00:00:00
abstract：:The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.0025
更新日期：1995-11-01 00:00:00
abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90178-9
更新日期：1988-09-01 00:00:00
abstract：:To determine whether low oxygen is a stimulus for endothelial cell differentiation and vascular development in the kidney, we examined the effect of low oxygen on rat metanephric organ culture, a model known to recapitulate nephrogenesis in the absence of vessels. After 6 days in culture in standard (20% O2) or low ox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8513
更新日期：1997-03-15 00:00:00
abstract：:Retinal stem cells (RSCs) exist as rare pigmented ciliary epithelial cells in adult mammalian eyes. We hypothesized that RSCs are at the top of the retinal cell lineage. Thus, genes expressed early in embryonic development to establish the retinal field in forebrain neuroectoderm may play important roles in RSCs. Pax6...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.021
更新日期：2007-04-15 00:00:00
abstract：:The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8764
更新日期：1997-12-15 00:00:00
