Abstract：
:Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle of the body being sure that the salivary glands were divided in two halves. Some of the ligated larvae had the anterior portion of the body dissected immediately in order to determine the puff pattern at the time of ligature. These patterns were compared with those from the posterior half which were dissected at different times after ligature. The results obtained in these experiments can be summarized as follow: (1) the absence of ecdysterone source prevents the induction of late puffs even when ligature is carried out after the induction of the early ecdysterone-responsive puffs; (2) the lack of an ecdysterone source blocks the process of gene amplification when the ligature is carried out at the beginning of this process (L3 stage); (3) the gene amplification process would not seem to be blocked but DNA puffs do not expand if the larvae are ligated some hours later (early L4 stage); (4) both gene amplification and DNA puffs expansion occur normally when larvae are ligated at a more advanced stage (at late L4 stage or thereafter). These results show that the presence of ecdysterone source is necessary for the induction of the late puffs up to a critical period at L4 stage. They also show that gene amplification and transcription of amplified sequences at the DNA puffs are two independently controlled phenomena.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Amabis DC,Amabis JMdoi
10.1016/0012-1606(84)90170-2subject
Has Abstractpub_date
1984-03-01 00:00:00pages
10-20issue
1eissn
0012-1606issn
1095-564Xpii
0012-1606(84)90170-2journal_volume
102pub_type
杂志文章abstract：:The current academic and business landscape does not provide a clear path for doctoral trainees graduating. Moreover, the fundamental need of the hour is diversification of the workforce where PhD graduates need to be trained to do more beyond basic scientific research. One of the possible paths that is now becoming p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.025
更新日期：2020-03-01 00:00:00
abstract：:The Drosophila larval central brain contains about 10,000 differentiated neurons and 200 scattered neural progenitors (neuroblasts), which can be further subdivided into ~95 type I neuroblasts and eight type II neuroblasts per brain lobe. Only type II neuroblasts generate self-renewing intermediate neural progenitors ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.10.020
更新日期：2012-01-01 00:00:00
abstract：:Mouse mammary ductal cells cultured in type I collagen gels give rise to three-dimensional multicellular outgrowths consisting of thin spikes which are often branched, and which may have pointed or blunt ends. The significance of these spikes to normal ductal morphogenesis has been unclear, since identical structures ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90036-8
更新日期：1984-07-01 00:00:00
abstract：:About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8972
更新日期：1998-09-01 00:00:00
abstract：:We have used amphibian gastrulation as a model system to study the action of the extracellular matrix (ECM) glycoprotein tenascin on mesodermal cell migration. Tenascin function was assayed in vitro during spreading of isolated cells from the dorsal marginal zone (DMZ) and during cell migration from DMZ explants. Plas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90256-i
更新日期：1990-02-01 00:00:00
abstract：:Cdc42, a member of the Rho family of GTP binding proteins, functions in the formation of polarized actin structures, in elongation of cell shape, and in cell signaling. Although genetic mutations previously have not been available in multicellular organisms, studies have attempted to discern Cdc42 functions in organis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9671
更新日期：2000-05-01 00:00:00
abstract：:The distribution of microtubules and microtubule organizing centers in the events leading up to the establishment of the first asymmetric cleavage furrow in nematode embryos was followed using indirect immunofluorescence of antibodies to tubulin. Oocytes arrest in meiotic prophase then undergo two meiotic reduction di...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90117-9
更新日期：1984-01-01 00:00:00
abstract：:To investigate the spatiotemporal dynamics of skin pattern formation, we developed a simple method for artificially disarranging the placement of all three pigment cell types in the body trunk of zebrafish (Danio rerio). We generated transgenic fish with melanophores that ectopically expressed a variant of channelrhod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.12.019
更新日期：2020-04-01 00:00:00
abstract：:The sea urchin sperm receptor isolated from the eggs of Strongylocentrotus purpuratus is a high molecular weight proteoglycan-like molecule. Previous studies in our laboratory suggested that the sperm receptor has two functional components, glycosaminoglycan chains that are responsible for sperm binding and polypeptid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90088-6
更新日期：1986-11-01 00:00:00
abstract：:ragged seedling2 (rgd2) is a novel, recessive mutation affecting lateral organ development in maize. The mutant phenotype of homozygous rgd2-R leaves is variable. Mild leaf phenotypes have a reduced midrib and may be moderately narrow and furcated; severe Rgd2-R(-) leaves are filamentous or even radial. Despite their ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.027
更新日期：2005-06-15 00:00:00
abstract：:The dorsal-ventral axis of the Xenopus embryo is approximately determined by the point of sperm entry, which occurs randomly about the animal-vegetal axis of the egg. Two cytoplasmic components were studied whose spatial arrangements and local concentration patterns appear to reflect the forming dorsal-ventral axis. F...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90457-9
更新日期：1985-06-01 00:00:00
abstract：:Nonmuscle myosin-II is a key motor protein that drives cell shape change and cell movement. Here, we analyze the function of nonmuscle myosin-II during Drosophila embryonic myogenesis. We find that nonmuscle myosin-II and the adhesion molecule, PS2 integrin, colocalize at the developing muscle termini. In the paradigm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0452
更新日期：2001-11-15 00:00:00
abstract：:The growing pollen tubes of tomato and tobacco were exposed to electrical fields and the effect on the direction of growth was measured. They responded by turning toward the positive electrode (anode) and gave a detectable response in fields as small as 0.1 mV/tube diameter. The sites of germination of tobacco pollen ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90266-2
更新日期：1989-12-01 00:00:00
abstract：:We studied the pathway of 5S ribosomal RNA (rRNA) during oogenesis in Xenopus from its storage in the cytoplasm to incorporation into ribosomes in the nucleus. Ribonucleoprotein particle (RNP) assembly assays reveal striking differences in the behavior of oocyte-type and somatic-type 5S rRNA after microinjection into ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1080
更新日期：1995-04-01 00:00:00
abstract：:Gap junctions are intercellular channels made of connexins (Cxs) that allow direct communication between adjacent cells. Modulation of Cxs has been associated with abnormal development and function of the mammary gland and breast cancer. However, the mechanisms underlying their expression during normal mammary gland a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.011
更新日期：2016-08-01 00:00:00
abstract：:Mammalian oocytes are arrested in meiotic prophase from around the time of birth until just before ovulation. Following an extended period of growth, they are stimulated to mature to the metaphase II stage by a preovulatory luteinizing hormone (LH) surge that occurs with each reproductive cycle. Small, growing oocytes...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.12.014
更新日期：2018-02-01 00:00:00
abstract：:Integrins are alphabeta transmembrane receptors that function in key cellular processes, including cell adhesion, differentiation, and extracellular matrix deposition through interactions with extracellular, membrane, and cytoplasmic proteins. We previously identified and cloned a muscle beta1 integrin cytoplasmic bin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00304-x
更新日期：2003-09-01 00:00:00
abstract：:GDF11, a new member of the TGF-beta gene superfamily, regulates anterior/posterior patterning in the axial skeleton during mouse embryogenesis. Gdf11 null mice display skeletal abnormalities that appear to represent anterior homeotic transformations of vertebrae consistent with high levels of Gdf11 expression in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9981
更新日期：2001-01-15 00:00:00
abstract：:BMPRIA is a receptor for bone morphogenetic proteins with high affinity for BMP2 and BMP4. Mouse embryos lacking Bmpr1a fail to gastrulate, complicating studies on the requirements for BMP signaling in germ layer development. Recent work shows that BMP4 produced in extraembryonic tissues initiates gastrulation. Here w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.048
更新日期：2004-06-01 00:00:00
abstract：:During Drosophila oogenesis, activation of Notch signaling in the follicular epithelium (FE) around stage 6 of oogenesis is essential for entry into the endocycle and a series of other changes such as cell differentiation and migration of subsets of the follicle cells. Notch induces the expression of zinc finger prote...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.04.024
更新日期：2014-08-01 00:00:00
abstract：:The hallmark of the arthropods is their segmented body, although origin of segmentation, however, is unresolved. In order to shed light on the origin of segmentation we investigated orthologs of pair rule genes (PRGs) and segment polarity genes (SPGs) in a member of the closest related sister-group to the arthropods, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.010
更新日期：2013-10-01 00:00:00
abstract：:The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90220-5
更新日期：1988-02-01 00:00:00
abstract：:Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9140
更新日期：1999-03-01 00:00:00
abstract：:During early development, GATA factors have been shown to be important for key events of coronary vasculogenesis, including formation of the epicardium. Myocardial GATA factors are required for coronary vascular (CV) formation; however, the role of epicardial localized GATAs in this process has not been addressed. The...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.033
更新日期：2014-02-01 00:00:00
abstract：:The outgrowth of motor axons to the developing pectoral fin of the Japanese medaka fish (Oryzias latipes) was investigated both in wildtype embryos and in the pectoral finless (pl) mutants in which adults are missing pectoral fins. Late in embryogenesis the pectoral fin is a simple limb which contains two antagonist m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90445-9
更新日期：1991-07-01 00:00:00
abstract：:Monoclonal antibodies (mAbs), electrophoresis, immunoblotting, and immunohistochemistry were used to determine the molecular properties of cardiac myosin heavy chain (MHC) isoforms and the regions of the developing chicken heart in which they were expressed. Adult atria expressed three electrophoretically distinct MHC...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90324-7
更新日期：1988-06-01 00:00:00
abstract：:Cells anchor to substrates by binding to extracellular matrix (ECM). In addition to this anchoring function however, cell-ECM binding is a mechanism for cells to sense their surroundings and to communicate and coordinate behaviour amongst themselves. Several ECM molecules and their receptors play essential roles in mu...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2011.03.015
更新日期：2011-06-15 00:00:00
abstract：:A complex regulatory cascade is required for normal cardiac development, and many aspects of this network are conserved from Drosophila to mammals. In Drosophila, the seven-up (svp) gene, an ortholog of the vertebrate chick ovalbumin upstream promoter transcription factors (COUP-TFI and II), is initially activated in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.025
更新日期：2007-02-15 00:00:00
abstract：:The engrailed gene is well known from its role in segmentation and central nervous system development in a variety of species. In molluscs, however, engrailed is involved in shell formation. So far, it seemed that engrailed had been co-opted uniquely for this particular process in molluscs. Here, we show that, in the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0653
更新日期：2002-06-15 00:00:00
abstract：:The mammalian prostate arises from the urogenital sinus and few factors have been identified to be important in the early stages of prostate development. In this study we show that the transcription factor Sox9 is expressed in the epithelia of all mouse prostatic lobes from the initial stages of their development. We ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.030
更新日期：2008-04-15 00:00:00
