Abstract：
:The mature heart valves and septa are derived from the cardiac cushions which initially form as local outgrowths of mesenchymal cells within the outflow tract and atrioventricular regions. Endocardial cells respond to signals from the overlying myocardium and undergo an epithelial-to-mesenchymal transformation to invade the intervening extracellular matrix. The molecules that can induce and maintain these cell populations are not known, but many candidates, including several TGFbetas and BMPs, have been proposed based on their expression patterns and activities in other systems. In the present study, we describe the expression of Bmp6 and Bmp7 in overlapping and adjacent sites, including the cardiac cushions during mouse embryonic development. Previous analyses demonstrate that neither of these BMPs is required during cardiogenesis, but analysis of Bmp6;Bmp7 double mutants uncovers a marked delay in the formation of the outflow tract endocardial cushions. A proportion of Bmp6;Bmp7 mutants also display defects in valve morphogenesis and chamber septation, and the embryos die between 10.5 and 15.5 dpc due to cardiac insufficiency. These data provide the first genetic evidence that BMPs are involved in the formation of the cardiac cushions.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Kim RY,Robertson EJ,Solloway MJdoi
10.1006/dbio.2001.0284subject
Has Abstractpub_date
2001-07-15 00:00:00pages
449-66issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(01)90284-2journal_volume
235pub_type
杂志文章abstract：:The correct assembly of junction components, such as E-cadherin and beta-catenin, into the zonula adherens is fundamental for the function of epithelia, both in flies and in vertebrates. In C. elegans, however, the cadherin-catenin system is not essential for general adhesion, raising the question as to the genetic ba...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0113
更新日期：2001-02-01 00:00:00
abstract：:Non-syndromic mitral valve prolapse (MVP) is the most common heart valve disease affecting 2.4% of the population. Recent studies have identified genetic defects in primary cilia as causative to MVP, although the mechanism of their action is currently unknown. Using a series of gene inactivation approaches, we define ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.03.003
更新日期：2020-07-01 00:00:00
abstract：:Retinal ganglion cells (RGCs) are the first cell type to differentiate during retinal histogenesis. It has been postulated that specified RGCs subsequently influence the number and fate of the remaining progenitors to produce the rest of the retinal cell types. However, several genetic knockout models have argued agai...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.01.015
更新日期：2008-04-15 00:00:00
abstract：:Integrins are expressed on the surface of some vertebrate eggs where they are thought to have a role in fertilization. The objective of this study is to determine if integrins are expressed on sea urchin eggs. The alphaB and betaC subunits were cloned using the homology polymerase chain reaction. Monoclonal and polycl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9910
更新日期：2000-11-15 00:00:00
abstract：:The extracorporeal vasculature of the colonial ascidian Botryllus schlosseri plays a key role in several biological processes: transporting blood, angiogenesis, regeneration, self-nonself recognition, and parabiosis. The vasculature also interconnects all individuals in a colony and is composed of a single layer of ec...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.10.013
更新日期：2019-04-15 00:00:00
abstract：:We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger(s) of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.11.005
更新日期：2019-01-15 00:00:00
abstract：:Changes in gene regulatory networks are a major engine for creating developmental novelty during evolution. Conversely, regulatory linkages that survive for very long evolutionary periods might be characteristic of ancient and abstract functions of fundamental utility to all metazoans. The proneural genes, which encod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.03.004
更新日期：2005-05-15 00:00:00
abstract：:To examine whether the extracellular matrix (ECM) plays a role in mediating interactions between the apical ectodermal ridge (AER) and the subjacent mesoderm in the chick wing bud, we used immunohistochemistry to locate the following tissue components during wing morphogenesis: types I and IV collagens, fibronectin, t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1141
更新日期：1994-05-01 00:00:00
abstract：:Recent results have demonstrated the occurrence of mRNA cap methylation in the sea urchin embryo following fertilization. It has been suggested that this methylation event is responsible for the translational activation of maternal histone mRNAs in these embryos. We have used aphidicolin, an effective inhibitor of bot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90161-8
更新日期：1987-05-01 00:00:00
abstract：:The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.004
更新日期：2009-06-15 00:00:00
abstract：:The cornea is one of the most densely innervated structures of the body. In the developing chicken embryo, nerves from the ophthalmic trigeminal ganglion (OTG) innervate the cornea in a series of spatially and temporally regulated events. However, little is known concerning the signals that regulate these events. Here...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.04.032
更新日期：2010-08-01 00:00:00
abstract：:The enteric nervous system (ENS) forms from the neural crest-derived precursors that colonize the bowel before differentiating into a network of neurons and glia that control intestinal function. Retinoids are essential for normal ENS development, but the role of retinoic acid (RA) metabolism in development remains in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.021
更新日期：2013-09-01 00:00:00
abstract：:Branching morphogenesis, the process by which cells or tissues generate tree-like networks that function to increase surface area or in contacting multiple targets, is a common developmental motif in multicellular organisms. We use Drosophila tracheal terminal cells, a component of the insect respiratory system, to in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.02.021
更新日期：2014-06-01 00:00:00
abstract：:Large steady electrical currents traverse Drosophila follicles in vitro as well as permeabilized eggs. During the period of main follicle growth (stages 9-11), these currents enter the anterior or nurse cell end of the follicles. This inward current acts like a sodium ion influx with some calcium involvement. During t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90013-2
更新日期：1985-03-01 00:00:00
abstract：:Artificial extracellular matrices composed of collagen, glycosaminoglycans (GAG), proteoglycans (PG), plasma fibronectin (FN), and a hyaluronate-binding protein (HABP) have been prepared that morphologically resemble embryonic extracellular matrices in vivo at the light and electron microscope level. The effect of eac...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/0012-1606(85)90461-0
更新日期：1985-06-01 00:00:00
abstract：:The inner ear, the sensory organ responsible for hearing and balance, contains specialized sensory and non-sensory epithelia arranged in a highly complex three-dimensional structure. To achieve this complexity, a tight coordination between morphogenesis and cell fate specification is essential during otic development....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.025
更新日期：2008-10-01 00:00:00
abstract：:Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9140
更新日期：1999-03-01 00:00:00
abstract：:Angiogenesis relies on specialized endothelial tip cells to extend toward guidance cues in order to direct growing blood vessels. Although many of the signaling pathways that control this directional endothelial sprouting are well known, the specific cellular mechanisms that mediate this process remain to be fully elu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.05.011
更新日期：2015-08-15 00:00:00
abstract：:The Egr family of zinc-finger transcription factors, consisting of Egr1, Egr2, Egr3, and Egr4, are involved in cellular growth and differentiation. Adult Egr3-deficient mice are ataxic and lack muscle spindle proprioceptors that normally develop at the sites of Ia afferent-myotube contacts during embryogenesis. To res...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0202
更新日期：2001-04-15 00:00:00
abstract：:The respective role of Pax2 and Pax8 in early kidney development in vertebrates is poorly understood. In this report, we have studied the roles of Pax8 and Pax2 in Xenopus pronephros development using a loss-of-function approach. Our results highlight a differential requirement of these two transcription factors for p...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.10.022
更新日期：2015-01-15 00:00:00
abstract：UNLABELLED:Notch signaling plays an acknowledged role in bile-duct development, but its involvement in cholangiocyte-fate determination remains incompletely understood. We investigated the effects of early Notch2 deletion in Notch2(fl/fl)/Alfp-Cre(tg/-) ("Notch2-cKO") and Notch2(fl/fl)/Alfp-Cre(-/-) ("control") mice. F...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.10.002
更新日期：2014-12-15 00:00:00
abstract：:Named for its assembly near exon-exon junctions during pre-mRNA splicing, the exon junction complex (EJC) regulates multiple aspects of RNA biochemistry, including export of spliced mRNAs from the nucleus and translation. Transcriptome analyses have revealed broad EJC occupancy of spliced metazoan transcripts, yet inh...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.09.010
更新日期：2020-01-01 00:00:00
abstract：:Recent studies with amoebae of the Myxomycetes Didymium iridis and Physarum polycephalum reveal that competence to undergo sexual fusion or to differentiate to plasmodia apogamically can be induced by a diffusible factor. Suspension cultures of Didymium amoebae have proven to be a good source of the inducer activity. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90337-3
更新日期：1984-06-01 00:00:00
abstract：:Bullous pemphigoid antisera and monoclonal antibodies to type VII collagen were used to localize hemidesmosomes and anchoring fibrils, respectively, in tissues of developing eyes and healing corneal wounds of New Zealand white rabbits. In the 17-day fetal rabbit eye, both antibodies colocalize to the epithelial-stroma...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90136-4
更新日期：1988-04-01 00:00:00
abstract：:Heparan sulfate proteoglycans (HSPGs) regulate a number of major developmental processes, but their roles in synovial joint formation remain unknown. Here we created conditional mouse embryo mutants lacking Ext1 in developing joints by mating Ext1(f/f) and Gdf5-Cre mice. Ext1 encodes a subunit of the Ext1/Ext2 Golgi-a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.12.022
更新日期：2011-03-01 00:00:00
abstract：:Nonmuscle myosin-II is a key motor protein that drives cell shape change and cell movement. Here, we analyze the function of nonmuscle myosin-II during Drosophila embryonic myogenesis. We find that nonmuscle myosin-II and the adhesion molecule, PS2 integrin, colocalize at the developing muscle termini. In the paradigm...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0452
更新日期：2001-11-15 00:00:00
abstract：:Electroporation-based assays were used to test whether the myogenic regulatory factor (MRF) of Ciona intestinalis (CiMRF) interferes with endogenous developmental programs, and to evaluate the importance of its unusual N-terminus for muscle development. We found that CiMRF suppresses both notochord and endoderm develo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.10.010
更新日期：2019-04-15 00:00:00
abstract：:The cranial neural crest (CNC) is a highly motile and multipotent embryonic cell population, which migrates directionally on defined routes throughout the embryo, contributing to facial structures including cartilage, bone and ganglia. Cadherin-mediated cell-cell adhesion is known to play a crucial role in the directi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.02.007
更新日期：2016-03-15 00:00:00
abstract：:Genome integrity in the developing germ line is strictly required for fecundity. In proliferating somatic cells and in germ cells, there are mitotic checkpoint mechanisms that ensure accurate chromosome segregation and euploidy. There is growing evidence of mitotic cell cycle components that are uniquely required in t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.03.011
更新日期：2015-06-15 00:00:00
abstract：:Autoradiography has been used to confirm and to extend previous microspectrophotometric studies (Doerder and DeBault, 1975) on the timing of DNA synthesis during conjugation in Tetrahymena thermophila. The majority of DNA synthesis occurs at the expected periods preceding gamete formation and the two postzygotic divis...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90500-8
更新日期：1987-12-01 00:00:00
