Abstract：
:Angiogenesis relies on specialized endothelial tip cells to extend toward guidance cues in order to direct growing blood vessels. Although many of the signaling pathways that control this directional endothelial sprouting are well known, the specific cellular mechanisms that mediate this process remain to be fully elucidated. Here, we show that Polo-like kinase 2 (PLK2) regulates Rap1 activity to guide endothelial tip cell lamellipodia formation and subsequent angiogenic sprouting. Using a combination of high-resolution in vivo imaging of zebrafish vascular development and a human umbilical vein endothelial cell (HUVEC) in vitro cell culture system, we observed that loss of PLK2 function resulted in a reduction in endothelial cell sprouting and migration, whereas overexpression of PLK2 promoted angiogenesis. Furthermore, we discovered that PLK2 may control angiogenic sprouting by binding to PDZ-GEF to regulate RAP1 activity during endothelial cell lamellipodia formation and extracellular matrix attachment. Consistent with these findings, constitutively active RAP1 could rescue the endothelial cell sprouting defects observed in zebrafish and HUVEC PLK2 knockdowns. Overall, these findings reveal a conserved PLK2-RAP1 pathway that is crucial to regulate endothelial tip cell behavior in order to ensure proper vascular development and patterning in vertebrates.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Yang H,Fang L,Zhan R,Hegarty JM,Ren J,Hsiai TK,Gleeson JG,Miller YI,Trejo J,Chi NCdoi
10.1016/j.ydbio.2015.05.011subject
Has Abstractpub_date
2015-08-15 00:00:00pages
49-60issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(15)00280-8journal_volume
404pub_type
杂志文章abstract：:Alkaline phosphatase (AP) activity is expressed in the endodermal cell lineage of ascidian embryos beginning at gastrulation. AP expression is resistant to levels of actinomycin D which completely suppress the appearance of other tissue-specific enzyme and morphological markers including acetylcholinesterase (AchE), a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90043-1
更新日期：1987-02-01 00:00:00
abstract：:Early embryonic cells are capable of acquiring numerous developmental fates until they become irreversibly committed to specific lineages depending on intrinsic determinants and/or regional interactions. From fertilization to gastrulation, such pluripotent cells first increase in number and then turn to undergoing dif...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2019.04.014
更新日期：2019-08-15 00:00:00
abstract：:The epithelial-mesenchymal transition of cardiac endothelium is a critical developmental event in the formation of valvular and septal anlagen. We have demonstrated previously that this event can be mimicked in culture by treating atrioventricular canal (AV) endothelium with EDTA-soluble proteins extracted from embryo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90121-i
更新日期：1991-06-01 00:00:00
abstract：:Establishment and maintenance of functional stem cells is critical for organ development and tissue homeostasis. Little is known about the mechanisms underlying stem establishment during organogenesis. Drosophila testes are among the most thoroughly characterized systems for studying stem cell behavior, with germline ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.09.009
更新日期：2012-12-01 00:00:00
abstract：:AKT signaling is an essential intracellular pathway controlling cell homeostasis, cell proliferation and survival, as well as cell migration and differentiation in adults. Alterations impacting the AKT pathway are involved in many pathological conditions in human disease. Similarly, during development, multiple transm...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.05.023
更新日期：2018-12-01 00:00:00
abstract：:The level of early histone mRNA in Strongylocentrotus purpuratus changes abruptly at 6 hr of development, increasing an average of 10-fold by 9-10.5 hr and then decreasing over 2-fold by 13.5-15 hr. These changes occur when the late embryonic mRNA is still a very minor component of histone gene transcription. The exac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90340-8
更新日期：1983-07-01 00:00:00
abstract：:Embryonic morphogenesis relies on the intrinsic ability of cells, often through remodeling the cytoskeleton, to shape epithelial tissues during development. Epithelial invagination is an example of morphogenesis that depends on this remodeling but the cellular mechanisms driving arrangement of cytoskeletal elements ne...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.02.014
更新日期：2020-06-01 00:00:00
abstract：:Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1093
更新日期：1995-04-01 00:00:00
abstract：:Unidirectional blue light directs the rhizoid-thallus axis in the apolar zygotes of Fucus and Pelvetia. Here, it is shown that blue light (but not red light) increased cyclic GMP levels of Pelvetia zygotes by about a factor of 2. When the increase in cyclic GMP was blocked by a guanylyl cyclase inhibitor, photopolariz...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8600
更新日期：1997-07-01 00:00:00
abstract：:Neural crest cells migrate along two pathways in the trunk: the ventral path, between the neural tube and somite, and the dorsolateral path, between the somite and overlying ectoderm. In avian embryos, ventral migration precedes dorsolateral migration by nearly 24 h, and the onset of dorsolateral migration coincides w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8963
更新日期：1998-08-15 00:00:00
abstract：:In mammals, two spatially and temporally distinct waves of fiber cell differentiation are crucial steps for normal lens development. In between these phases, an anterior growth zone forms in which progenitor cells migrate circumferentially, terminally exit the cell cycle and initiate differentiation at the lens equato...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.015
更新日期：2009-04-01 00:00:00
abstract：:A polarity in gap junctional permeability normally exists in 32-cell stage Xenopus embryos, in that dorsal cells are relatively more coupled than ventral cells, as measured by transfer of Lucifer yellow dye. The current study extends our analysis of whether gap junctional permeability at this stage can be modulated by...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90227-8
更新日期：1992-05-01 00:00:00
abstract：:We investigated the potential role of rostral-caudal and dorsal-ventral subdivisions of the early rostral brain by relating these subdivisions to the early patterning of neuron cell bodies and their axon projections. The earliest neurons were mapped using the lipophilic axon tracers diI and diO on embryos fixed on emb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0008
更新日期：1996-01-10 00:00:00
abstract：:Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct regions, exhibiting differences in m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.06.004
更新日期：2018-09-15 00:00:00
abstract：:Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.021
更新日期：2009-02-15 00:00:00
abstract：:The sensory neurons of the Drosophila wing differentiate during the initial stages of metamorphosis, appearing in the imaginal wing disc as it everts and flattens. These identifiable neurons arise in a stereotyped sequence, and lay down a specific pattern of axon bundles which travel proximally to the CNS. In several ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90044-2
更新日期：1985-04-01 00:00:00
abstract：:Embryonic kidney development begins with the outgrowth of the ureteric bud (UB) from the Wolffian duct (WD) into the adjacent metanephric mesenchyme (MM). Both a GDNF-dependent and GDNF-independent (Maeshima et al., 2007) pathway have been identified. In vivo and in vitro, the GDNF-dependent pathway is inhibited by BM...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.029
更新日期：2010-11-15 00:00:00
abstract：:Retinoic acid (RA) is a potent inducer of cell differentiation and plays an essential role in sex-specific germ cell development in the mammalian gonad. RA is essential for male gametogenesis and hence fertility. However, RA can also disrupt sexual cell fate in somatic cells of the testis, promoting transdifferentiati...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.02.015
更新日期：2017-04-15 00:00:00
abstract：:Runx2-Cbfa1, a Runt transcription factor, plays important roles during skeletal development. It is required for differentiation and function of osteoblasts. In its absence, chondrocyte hypertrophy is severely impaired and there is no vascularization of cartilage templates during skeletal development. These tissue-spec...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.003
更新日期：2004-06-15 00:00:00
abstract：:Actin-related protein 5 (ARP5) is a conserved subunit of the INO80 chromatin-remodeling complex in yeast and mammals. We have characterized the expression and subcellular distribution of Arabidopsis thaliana ARP5 and explored its role in the epigenetic control of multicellular development and DNA repair. ARP5-specific...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.08.006
更新日期：2009-11-01 00:00:00
abstract：:Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.05.006
更新日期：2020-08-01 00:00:00
abstract：:Catecholaminergic cells are transiently present during development of the fetal murine bowel. These transient catecholaminergic (TC) cells appear at Day E10, but by Day E13 can no longer be detected. In order to evaluate the hypothesis that these cells are the precursors of enteric neurons, we investigated the possibi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90217-0
更新日期：1989-03-01 00:00:00
abstract：:In mammalian fertilization, paternal chromatin is exhaustively remodeled, yet the maternal contribution to this process is unknown. To address this, we prevented the induction of meiotic exit by spermatozoa and examined sperm chromatin remodeling in metaphase II (mII) oocytes. Methylation of paternal H3-K4 and H3-K9 r...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.006
更新日期：2007-01-15 00:00:00
abstract：:Mutations impairing the GTPase activity of G protein Galpha subunits can result in activated Galpha subunits that affect signal transduction and cellular responses and, in some cases, promote tumor formation. An analogous mutation in the Dictyostelium Galpha4 subunit gene (Q200L substitution) was constructed and found...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9474
更新日期：1999-11-15 00:00:00
abstract：:In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8887
更新日期：1998-05-15 00:00:00
abstract：:We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1236
更新日期：1993-09-01 00:00:00
abstract：:Rhodopsin is essential for normal photoreceptor development in Drosophila (O'Tousa et al., 1989; Leonard et al., 1992; Kumar and Ready, 1995) and in mice (Humphries et al., 1997). Here we report studies in which a rhodopsin transgene is expressed at restricted stages during the development of Drosophila photoreceptors...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8636
更新日期：1997-08-01 00:00:00
abstract：:Nematodes provide a useful experimental system with which to investigate the evolution of development at the cellular, genetic, and molecular levels. Building on an understanding of vulval development in Caenorhabditis elegans, analysis of vulval development has been extended to a number of other species in three fami...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1006/dbio.1996.0035
更新日期：1996-02-01 00:00:00
abstract：:Proper patterning and growth of oral structures including teeth, tongue, and palate rely on epithelial-mesenchymal interactions involving coordinated regulation of signal transduction. Understanding molecular mechanisms underpinning oral-facial development will provide novel insights into the etiology of common congen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.002
更新日期：2011-08-01 00:00:00
abstract：:In this study, we present evidence that neurogenic cells inhibit the differentiation of cardiogenic cells. When cells of the entire area pellucida at stage 5 were dissociated and reaggregated, the aggregates differentiated into neural tissues and other structures of any germ layer origin, except for heart tissues, des...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1266
更新日期：1995-09-01 00:00:00
