Abstract：
:Genome integrity in the developing germ line is strictly required for fecundity. In proliferating somatic cells and in germ cells, there are mitotic checkpoint mechanisms that ensure accurate chromosome segregation and euploidy. There is growing evidence of mitotic cell cycle components that are uniquely required in the germ line to ensure genome integrity. We previously showed that the primary phenotype of germ cell deficient 2 (gcd2) mutant mice is infertility due to germ cell depletion during embryogenesis. Here we show that the underlying mutation is a mis-sense mutation, R308K, in the motor domain of the kinesin-8 family member, KIF18A, a protein that is expressed in a variety of proliferative tissues and is a key regulator of chromosome alignment during mitosis. Despite the conservative nature of the mutation, we show that its functional consequences are equivalent to KIF18A deficiency in HeLa cells. We also show that somatic cells progress through mitosis, despite having chromosome alignment defects, while germ cells with similar chromosome alignment defects undergo mitotic arrest and apoptosis. Our data provide evidence for differential requirements for chromosome alignment in germ and somatic cells and show that Kif18a is one of a growing number of genes that are specifically required for cell cycle progression in proliferating germ cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Czechanski A,Kim H,Byers C,Greenstein I,Stumpff J,Reinholdt LGdoi
10.1016/j.ydbio.2015.03.011subject
Has Abstractpub_date
2015-06-15 00:00:00pages
253-262issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(15)00144-Xjournal_volume
402pub_type
杂志文章abstract：:We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells wi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0537
更新日期：2002-02-15 00:00:00
abstract：:The segmental origin and migratory pattern of neural crest cells at the trunk level of avian embryos was studied, with special emphasis on the formation of the dorsal root ganglia (DRG) which organize in the anterior half of each somite. Neural crest cells were visualized using the quail-chick marker and HNK-1 immunof...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90236-3
更新日期：1987-04-01 00:00:00
abstract：:Repulsion plays a fundamental role in the establishment of a topographic map of the chick retinotectal projections. This has been highlighted by studies demonstrating the role of opposing gradients of the EphA3 receptor tyrosine kinase on retinal axons and two of its ligands, ephrin-A2 and ephrin-A5, in the tectum. We...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0268
更新日期：2001-06-15 00:00:00
abstract：:Members of the fibroblast growth factor (FGF) family of peptide growth factors are widely expressed in the germ layer derivatives during gastrulation and early organogenesis of the mouse. We have investigated the effect of administering recombinant FGF-4 in the late-primitive streak stage embryo to test if the pattern...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9663
更新日期：2000-05-01 00:00:00
abstract：:The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8764
更新日期：1997-12-15 00:00:00
abstract：:Eggs have developed their own strategies for early development. Amphibian, teleost fish, and ascidian eggs show cortical rotation and an accompanying structure, a cortical parallel microtubule (MT) array, during the one-cell embryonic stage. Cortical rotation is thought to relocate maternal deposits to a certain compa...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.11.019
更新日期：2019-04-15 00:00:00
abstract：:Trypsin-like activity is secreted from eggs of many species at fertilization, and this activity is believed to be critical for the block to polyspermy. Here we show that a cortical granule serine protease of sea urchins is the major and perhaps only protease family member important for fertilization. Zymography assays...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9299
更新日期：1999-07-01 00:00:00
abstract：:In the deuterostomes and ecdysozoans that have been studied (e.g. chordates and insects), neural fate specification relies on signaling from surrounding cells. However, very little is known about mechanisms of neural specification in the third major bilaterian clade, spiralians. Using blastomere isolation in the annel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.09.022
更新日期：2017-11-15 00:00:00
abstract：:In tetrapods, the anteroposterior (AP) patterning of the limb is under the control of the antagonistic activities of the secreted factor Sonic hedgehog (Shh) and Gli3R, the truncated repressor form of the transcription factor Gli3. In this report, we show that Msx1 and Msx2 are targets and downstream effectors of Gli3...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.038
更新日期：2009-07-15 00:00:00
abstract：:Bone development is dependent on the functionality of three essential cell types: chondrocytes, osteoclasts and osteoblasts. If any of these cell types is dysfunctional, a developmental bone phenotype can result. The bone disease osteopetrosis is caused by osteoclast dysfunction or impaired osteoclastogenesis, leading...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.06.018
更新日期：2010-08-15 00:00:00
abstract：:The ectoderm of early Xenopus gastrula is competent to become induced to neural tissue, but dorsal ectoderm is more neural competent than ventral ectoderm. It is a tenable, but as yet untested possibility that the higher neural competence of dorsal gastrula ectoderm is dependent on the presence of the dorsal mesoderm....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90168-g
更新日期：1992-07-01 00:00:00
abstract：:Adult tissue stem cells self-renew and differentiate in a way that exactly meets the biological demand of the dependent tissue. We evaluated spermatogonial stem cell (SSC) activity in the developing rat testis and the quality and accessibility of the stem cell niche in wild type, and two busulfan-treated models of rat...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.07.010
更新日期：2003-11-15 00:00:00
abstract：:Integrins are expressed on the surface of some vertebrate eggs where they are thought to have a role in fertilization. The objective of this study is to determine if integrins are expressed on sea urchin eggs. The alphaB and betaC subunits were cloned using the homology polymerase chain reaction. Monoclonal and polycl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9910
更新日期：2000-11-15 00:00:00
abstract：:Ooplasmic segregation in the late interphase zygote of the leech Theromyzon trizonare is accomplished by reorganization of an ectoplasmic cytoskeleton formed by polar rings and meridional bands. The dynamic properties of this cytoskeleton were explored by time-lapse confocal and video microscopy. Cytoskeleton assembly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.023
更新日期：2004-07-01 00:00:00
abstract：:A gene regulatory network subcircuit comprising the otx, wnt8, and blimp1 genes accounts for a moving torus of gene expression that sweeps concentrically across the vegetal domain of the sea urchin embryo. Here we confirm by mutation the inputs into the blimp1cis-regulatory module predicted by network analysis. Its es...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.10.042
更新日期：2008-01-15 00:00:00
abstract：:In the development of mammalian organs, a rapid and robust expansion of the parenchymal compartment must occur following allocation of organ primordia. This expansion must be regulated so that sufficient tissue mass is generated for further organization into functional tissues. The discovery that mosaic patches in the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1274
更新日期：1994-10-01 00:00:00
abstract：:Collapsin-1 is a secreted glycoprotein that inhibits the extension of specific growth cones in vitro. It has been hypothesized to serve as a repulsive guidance cue for extending growth cones in vivo. Here we report the distribution of collapsin-1 message as demonstrated by in situ hybridization using digoxigenin-label...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0016
更新日期：1996-01-10 00:00:00
abstract：:Gastrulation is the first major morphogenetic movement in development and requires dynamic regulation of cell adhesion and the cytoskeleton. Caenorhabditis elegans gastrulation begins with the migration of the two endodermal precursors, Ea and Ep, from the surface of the embryo into the interior. Ea/Ep migration provi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.507
更新日期：2010-08-15 00:00:00
abstract：:Fertilization results in the tyrosine phosphorylation of several egg proteins within minutes of sperm-egg binding and inhibitor studies have shown that tyrosine kinase activity is required for many aspects of egg activation. The present study demonstrates the presence of p59c-fyn kinase in the sea urchin egg and exami...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0073
更新日期：1996-03-15 00:00:00
abstract：:The remarkable regenerative capacity displayed by plants and various vertebrates, such as amphibians, is largely based on the capability of somatic cells to undergo dedifferentiation. In this process, mature cells reverse their state of differentiation and acquire pluripotentiality--a process preceding not only reentr...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2003.12.027
更新日期：2004-04-01 00:00:00
abstract：:A small number of signaling pathways are used repeatedly during organogenesis, and they can have drastically different effects on the same population of cells depending on the embryonic stage. How cellular competence changes over developmental time is not well understood. Here we used Xenopus, mouse, and human pluripo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.11.018
更新日期：2018-02-01 00:00:00
abstract：:The germinal vesicle (GV) of starfish oocytes contains a factor which is required to drive the cytoplasmic cycle of the meiotic division. Biochemical investigation of this factor has been difficult due to the small quantities of obtainable GV materials. To overcome this, we have developed a mass-isolation procedure fo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90187-l
更新日期：1992-06-01 00:00:00
abstract：:Early spermatids contain roughly 1000-fold more TATA-binding protein (TBP) mRNA than do somatic cells. The appearance of TBP-overexpressing spermatids in the developing testis is accompanied by a large increase in whole-organ levels of total RNA and of poly(A)+ RNA per cell. Whereas somatic cells initiate transcriptio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8514
更新日期：1997-04-01 00:00:00
abstract：:The fertilized ascidian egg is thought to be comprised of distinct regions of tissue-specific cytoplasmic determinants. This idea was tested by bisecting fertilized eggs into egg fragments and culturing them until the unoperated controls developed into larvae. Fertilized eggs were bisected using a microsurgical method...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90178-9
更新日期：1988-09-01 00:00:00
abstract：:Segmentation is a key step in embryonic development. Acting in all germ layers, it is responsible for the generation of antero-posterior asymmetries. Hox genes, with their diverse expression in individual segments, are fundamental players in the determination of different segmental fates. In vertebrates, Hox gene prod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.04.016
更新日期：2010-07-01 00:00:00
abstract：:In the Drosophila ventral nerve cord, the three pairs of Capability neuropeptide-expressing Va neurons are exclusively found in the second, third and fourth abdominal segments (A2-A4). To address the underlying mechanisms behind such segment-specific cell specification, we followed the developmental specification of t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.02.015
更新日期：2011-05-01 00:00:00
abstract：:Early embryonic cells are capable of acquiring numerous developmental fates until they become irreversibly committed to specific lineages depending on intrinsic determinants and/or regional interactions. From fertilization to gastrulation, such pluripotent cells first increase in number and then turn to undergoing dif...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2019.04.014
更新日期：2019-08-15 00:00:00
abstract：:In mammals, two spatially and temporally distinct waves of fiber cell differentiation are crucial steps for normal lens development. In between these phases, an anterior growth zone forms in which progenitor cells migrate circumferentially, terminally exit the cell cycle and initiate differentiation at the lens equato...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.015
更新日期：2009-04-01 00:00:00
abstract：:In amphibians, the composition and pattern of expression of the myosin heavy chain (myHC) gene family are mostly unknown. To understand better the regulation of this complex family, we screened cDNA libraries from swimming tadpole and adult leg muscle RNA and have isolated and partially sequenced clones for several my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90058-4
更新日期：1989-06-01 00:00:00
abstract：:FoxC, FoxF, FoxL1 and FoxQ1 genes have been shown to be clustered in some animal genomes, with mesendodermal expression hypothesised as a selective force maintaining cluster integrity. Hypotheses are, however, constrained by a lack of data from the Lophotrochozoa. Here we characterise members of the FoxC, FoxF, FoxL1 ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.01.015
更新日期：2010-04-15 00:00:00
