Abstract：
:Dictyostelium discoideum amoebas display colonial multicellularity where starving amoebas aggregate to form migrating slugs and fruiting bodies consisting of spores and three supporting cell types. To resolve the cell signalling mechanism that control sporulation, we use insertional mutagenesis of amoebas transformed with fusion constructs of spore genes and red fluorescent protein. We identified the defective gene in a mutant lacking spore gene expression as the autophagy gene Atg7. Directed knock-out of atg7 and of autophagy genes like atg5 and atg9 yielded a similar phenotype, with lack of viable spores and excessive differentiation of stalk cells. The atg7-, atg5- and atg9- cells were specifically defective in cAMP induction of prespore genes, but showed enhanced cAMP stimulation of prestalk genes at the same developmental stage. The lack of prespore gene induction in the autophagy mutants was not due to deleterious effects of loss of autophagy on known components of the cAMP pathway, such as cAMP receptors and their cAMP-induced phosphorylation and internalization, PKA and the transcription factors SpaA and GbfA, or to lack of NH3 production by proteolysis, which was previously suggested to stimulate the spore pathway. Our continued mutagenesis approach is the most likely to yield the intriguing link between autophagy and prespore gene induction.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Yamada Y,Schaap Pdoi
10.1016/j.ydbio.2019.04.017subject
Has Abstractpub_date
2019-08-15 00:00:00pages
114-126issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(19)30028-4journal_volume
452pub_type
杂志文章abstract：:Peptide growth factors can initiate changes in cell fate in Xenopus ectodermal explants and induce the formation of mesoderm. Marker genes expressed in mesoderm allow the analysis of whether, or how much, induction has occurred, but do not tell us what molecules are involved in carrying out the response. In this repor...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0158
更新日期：1996-07-10 00:00:00
abstract：:Dorsoventral specification of the zebrafish gastrula is governed by the functions of the dorsal shield, a region of the embryo functionally analogous to the amphibian Spemann organizer. We report that the bozozok locus encodes the transcription factor nieuwkoid/dharma, a homeobox gene with non-cell-autonomous organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9479
更新日期：1999-11-15 00:00:00
abstract：:We recently demonstrated the requirement of two-pore channel type 2 (TPC2)-mediated Ca2+ release during slow muscle cell differentiation and motor circuit maturation in intact zebrafish embryos. However, the upstream trigger(s) of TPC2/Ca2+ signaling during these developmental processes remains unclear. Nicotinic acid...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.11.005
更新日期：2019-01-15 00:00:00
abstract：:The germinal vesicle (GV) of starfish oocytes contains a factor which is required to drive the cytoplasmic cycle of the meiotic division. Biochemical investigation of this factor has been difficult due to the small quantities of obtainable GV materials. To overcome this, we have developed a mass-isolation procedure fo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90187-l
更新日期：1992-06-01 00:00:00
abstract：:The thisbe (ths) gene encodes a Drosophila fibroblast growth factor (FGF), and mutant females are viable but sterile suggesting a link between FGF signaling and fertility. Ovaries exhibit abnormal morphology including lack of epithelial sheaths and muscle tissues that surround ovarioles. Here we investigated how FGF i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.04.023
更新日期：2015-08-01 00:00:00
abstract：:lilliputian (lilli), the sole Drosophila member of the FMR2/AF4 (Fragile X Mental Retardation/Acute Lymphoblastic Leukemia) family of transcription factors, is widely expressed with roles in segmentation, cellularization, and gastrulation during early embryogenesis with additional distinct roles at later stages of emb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.10.027
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abstract：:The enteric nervous system (ENS) forms from the neural crest-derived precursors that colonize the bowel before differentiating into a network of neurons and glia that control intestinal function. Retinoids are essential for normal ENS development, but the role of retinoic acid (RA) metabolism in development remains in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.06.021
更新日期：2013-09-01 00:00:00
abstract：:In mammals, two spatially and temporally distinct waves of fiber cell differentiation are crucial steps for normal lens development. In between these phases, an anterior growth zone forms in which progenitor cells migrate circumferentially, terminally exit the cell cycle and initiate differentiation at the lens equato...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.015
更新日期：2009-04-01 00:00:00
abstract：:The formation and maintenance of the highly regular organization of membrane systems and proteins in striated muscle require specific membrane-membrane and membrane-cytoskeleton interactions. The development of T-tubules and sarcoplasmic reticulum (SR) was followed in gastrocnemius muscle fibers from chicken embryos b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1292
更新日期：1993-11-01 00:00:00
abstract：:The murine cpk mouse develops a rapid-onset polycystic kidney disease (PKD) with many similarities to human PKD. During kidney development, the transcription factor Pax2 is required for the specification and differentiation of the renal epithelium. In humans, Pax2 is also expressed in juvenile cystic kidneys where it ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9618
更新日期：2000-03-15 00:00:00
abstract：:Bone morphogenetic protein (BMP) signalling is key to many developmental processes, including early regionalisation of the ectoderm. The neural crest is induced here by a combination of BMP and Wnt signals from nearby tissues with many secreted factors contributing to its initial specification at the neural plate bord...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.12.015
更新日期：2020-04-15 00:00:00
abstract：:Successful fertilization requires that sperm are activated prior to contacting an oocyte. In C. elegans, this activation process, called spermiogenesis, transforms round immobile spermatids into motile, fertilization-competent spermatozoa. We describe the phenotypic and genetic characterization of spe-43, a new compon...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.02.013
更新日期：2018-04-15 00:00:00
abstract：:Meckel-Gruber syndrome (MKS) is an embryonic lethal ciliopathy resulting from mutations in genes encoding proteins localising to the primary cilium. Mutations in the basal body protein MKS1 account for 7% of cases of MKS. The condition affects the development of multiple organs, including brain, kidney and skeleton. H...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.02.015
更新日期：2013-05-01 00:00:00
abstract：:The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present st...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90188-7
更新日期：1989-10-01 00:00:00
abstract：:Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.08.002
更新日期：2020-10-01 00:00:00
abstract：:VegT is an essential maternal regulator of germ layer specification in Xenopus. The localization of VegT mRNA to the vegetal cortex of the oocyte during oogenesis ensures its inheritance by vegetal and not animal cells, and directs the differentiation of vegetal cells into endoderm. Similarly localized mRNAs, Vg1 and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0495
更新日期：2001-12-15 00:00:00
abstract：:The organizer is an essential signaling center required for axial formation during vertebrate embryonic development. In the basal chordate amphioxus, the dorsal blastopore lip of the gastrula has been proposed to be homologous to the vertebrate organizer. Lefty is one of the first genes to be expressed in the organize...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.08.006
更新日期：2019-12-01 00:00:00
abstract：:Fibroblast growth factor (FGF) signalling has been implicated in the generation of mesoderm and neural fates in chordate embryos including ascidians and vertebrates. In Ciona, FGF9/16/20 has been implicated in both of these processes. However, in FGF9/16/20 knockdown embryos, notochord fate recovers during later devel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.08.075
更新日期：2007-02-01 00:00:00
abstract：:The heart is the first organ to form and function in the vertebrate embryo. Furthermore, differences between the left and right sides of the embryo become first detectable during cardiac development. We observed strong cardiac laterality phenotypes in medaka embryos by manipulating Groucho protein activity. The phenot...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.11.020
更新日期：2007-03-01 00:00:00
abstract：:The mouse prostate gland develops by branching morphogenesis from the urogenital epithelium and mesenchyme. Androgens and developmental factors, including FGF10 and SHH, promote prostate growth (Berman, D.M., Desai, N., Wang, X., Karhadkar, S.S., Reynon, M., Abate-Shen, C., Beachy, P.A., Shen, M.M., 2004. Roles for He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.08.018
更新日期：2005-12-15 00:00:00
abstract：:Spermatogonial stem cells (SSCs) must balance self-renewal with production of transit-amplifying progenitors that differentiate in response to retinoic acid (RA) before entering meiosis. This self-renewal vs. differentiation spermatogonial fate decision is critical for maintaining tissue homeostasis, as imbalances cau...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90170-2
更新日期：1984-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.12.017
更新日期：2013-03-15 00:00:00
abstract：:During development of the vertebrate visual system, retinal ganglion cell (RGC) axons follow a precise path toward their midbrain targets. Although much is known about the cues that direct RGC axons once they have left the optic disc, less is known about the guidance of axons at earlier stages, when RGCs first send ou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9312
更新日期：1999-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1266
更新日期：1995-09-01 00:00:00
abstract：:The interaction of heterologous tissues involves cell adhesion mediated by the extracellular matrix and its receptor integrins. The Drosophila wing disc is an ectodermal invagination that contacts specific tracheal branches at the basolateral cell surface. We show that an alpha subunit of laminin, encoded by wing blis...
journal_title：Developmental biology
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doi：10.1016/j.ydbio.2006.12.022
更新日期：2007-04-01 00:00:00
abstract：:Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8592
更新日期：1997-06-15 00:00:00
abstract：:A chronic neuromuscular blockade during those embryonic stages when naturally occurring spinal motoneuron death occurs, results in the prevention of this cell loss. The excess motoneurons are maintained as long as the neuromuscular blockade is continued; once embryonic neuromuscular activity resumes, however, the exce...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90114-3
更新日期：1984-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90445-3
更新日期：1987-09-01 00:00:00
abstract：:beta-Catenin was originally identified biochemically as a protein that binds E-cadherin in cultured cells and that interaction was later shown to be essential for cadherin function. Independently, armadillo, the beta-catenin homolog in Drosophila melanogaster, was identified as a segment polarity gene necessary for th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8739
更新日期：1997-12-15 00:00:00
