Abstract：
:The thisbe (ths) gene encodes a Drosophila fibroblast growth factor (FGF), and mutant females are viable but sterile suggesting a link between FGF signaling and fertility. Ovaries exhibit abnormal morphology including lack of epithelial sheaths and muscle tissues that surround ovarioles. Here we investigated how FGF influences Drosophila ovary morphogenesis and identified several roles. Heartless (Htl) FGF receptor was found to be expressed within somatic cells at the larval and pupal stages, and phenotypes were uncovered using RNAi. Differentiation of terminal filament cells was affected, but this effect did not alter the ovariole number. In addition, proliferation of epithelial sheath progenitors, the apical cells, was decreased in both htl and ths mutants, while ectopic expression of the Ths ligand led to these cells' over-proliferation suggesting that FGF signaling supports ovarian muscle sheath formation by controlling apical cell number in the developing gonad. Additionally, live imaging of adult ovaries was used to show that htl RNAi mutants, hypomorphic mutants in which epithelial sheaths are present, exhibit abnormal muscle contractions. Collectively, our results demonstrate that proper formation of ovarian muscle tissues is regulated by FGF signaling in the larval and pupal stages through control of apical cell proliferation and is required to support fertility.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Irizarry J,Stathopoulos Adoi
10.1016/j.ydbio.2015.04.023subject
Has Abstractpub_date
2015-08-01 00:00:00pages
1-13issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(15)00245-6journal_volume
404pub_type
杂志文章abstract：:Several genomic clones were isolated from a Drosophila library screened with cDNA prepared from abundant adult female mRNA. Cytoplasmic dot hybridizations have shown that the genes in all of these clones are expressed only in posteclosion (stages 8-14) follicles. One set of overlapping clones (lambda 20, lambda 28, an...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90271-9
更新日期：1984-09-01 00:00:00
abstract：:Sonic hedgehog is a secreted factor regulating patterning of the anterior-posterior axis in the developing limb. The signaling pathway mediating the transduction of the signal is still poorly understood. In Drosophila several genes are known to act downstream of hedgehog, the fly homolog of Sonic hedgehog. An importan...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0300
更新日期：1996-11-25 00:00:00
abstract：:TGF-beta superfamily members signal through a heteromeric receptor complex to regulate craniofacial development. TGF-beta type II receptor appears to bind only TGF-beta, whereas TGF-beta type I receptor (ALK5) also binds to ligands in addition to TGF-beta. Our previous work has shown that conditional inactivation of T...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.03.045
更新日期：2008-08-01 00:00:00
abstract：:An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8998
更新日期：1998-10-15 00:00:00
abstract：:A group of proteins judged on several criteria to be yolk proteins have been isolated from a homogenate of the nematode Caenorhabditis elegans. Comparison of partial proteolysis fragments indicates that the two bands of a 170,000-dalton doublet (yp170) are closely related; bands observed at 115,000 daltons (yp115) and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90321-4
更新日期：1983-03-01 00:00:00
abstract：:Metamorphosis involves the destruction of larval, the formation of adult and the transformation of larval into adult tissues. In this study, we demonstrate the role of the Drosophila nuclear proteins EAST and Chromator in tissue destruction and remodeling. To better understand the function of east, we performed a yeas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.05.001
更新日期：2007-07-15 00:00:00
abstract：:We recently discovered that in rat spermatids, kinesin light chain KLC3 can associate with outer dense fibers, major sperm tail components, and accumulates in the sperm midpiece. Here, we show that mitochondria isolated from rat-elongating spermatids have bound KLC3. Immunoelectron microscopy indicates that the associ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.014
更新日期：2004-11-01 00:00:00
abstract：:The facial primordia in the chick embryo begin as rounded swellings that surround the primitive mouth and these grow out to form the beak. The control of proximodistal outgrowth is not well understood but may involve similar mechanisms to the limb bud. In order to test this hypothesis, combinations were made between e...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90069-s
更新日期：1992-12-01 00:00:00
abstract：:During epithelial development cells become polarized along their apical-basal axis and some epithelia also exhibit polarity in the plane of the tissue. Mutations in the gene encoding a Drosophila Pak family serine/threonine kinase, dPak, disrupt the follicular epithelium that covers developing egg chambers during ooge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.034
更新日期：2007-05-15 00:00:00
abstract：:C. elegans ahr-1 is orthologous to the mammalian aryl hydrocarbon receptor, and it functions as a transcription factor to regulate the development of certain neurons. Here, we describe the role of ahr-1 in a specific behavior: the aggregation of C. elegans on lawns of bacterial food. This behavior is modulated by nutr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.07.017
更新日期：2006-10-15 00:00:00
abstract：:Results of numerous experiments indicate that the transient rise in intracellular Ca2+ following sperm-egg fusion is essential for the subsequent events that constitute egg activation. Some events of egg activation, e.g., cortical granule exocytosis, however, appear more sensitive to intracellular Ca2+ than other even...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0331
更新日期：1996-12-15 00:00:00
abstract：:To examine the conditions of cell composition necessary for formation of a complete tadpole, defect embryos of 35 series were prepared by removing particular cells from 16- and 32-cell embryos of Xenopus laevis. These defect embryos were cultured and their development was examined. Formation of a complete tadpole did ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1223
更新日期：1995-08-01 00:00:00
abstract：:A small number of signaling pathways are used repeatedly during organogenesis, and they can have drastically different effects on the same population of cells depending on the embryonic stage. How cellular competence changes over developmental time is not well understood. Here we used Xenopus, mouse, and human pluripo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.11.018
更新日期：2018-02-01 00:00:00
abstract：:Unlike adult mammals, adult frogs regrow their optic nerve following a crush injury, making Xenopus laevis a compelling model for studying the molecular mechanisms that underlie neuronal regeneration. Using Translational Ribosome Affinity Purification (TRAP), a method to isolate ribosome-associated mRNAs from a target...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.003
更新日期：2017-06-15 00:00:00
abstract：:Immature, Stage VI oocytes of Xenopus laevis fail to activate (i.e., to propagate a cortical reaction and elevate a fertilization envelope) when pricked or exposed to A23187. We determined the times during maturation when immature oocytes treated with progesterone in vitro developed the capacity to respond to pricking...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90177-5
更新日期：1984-03-01 00:00:00
abstract：:Runx2-Cbfa1, a Runt transcription factor, plays important roles during skeletal development. It is required for differentiation and function of osteoblasts. In its absence, chondrocyte hypertrophy is severely impaired and there is no vascularization of cartilage templates during skeletal development. These tissue-spec...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.003
更新日期：2004-06-15 00:00:00
abstract：:The nucleus of eukaryotic cells is organized into functionally specialized compartments that are essential for the control of gene expression, chromosome architecture and cellular differentiation. The mouse oocyte nucleus or germinal vesicle (GV) exhibits a unique chromatin configuration that is subject to dynamic mod...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2006.01.008
更新日期：2006-04-01 00:00:00
abstract：:Germ line transformation has been used to map the cis regulatory DNA elements responsible for the precise and evolutionarily stable developmental expression of the s18 chorion gene. Constructs containing chimeric combinations of Drosophila melanogaster and D. grimshawi DNA regions, as well as D. grimshawi sequences al...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90160-i
更新日期：1992-07-01 00:00:00
abstract：:Kinesin light chain 3 (KLC3) is the only known kinesin light chain expressed in post-meiotic male germ cells. We have reported that in rat spermatids KLC3 associates with outer dense fibers and mitochondrial sheath. KLC3 is able to bind to mitochondria in vitro and in vivo employing the conserved tetratrico-peptide re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.04.026
更新日期：2012-06-15 00:00:00
abstract：:We have characterized the antigen recognized by mab10, a monoclonal antibody that has been shown to modify outgrowth of thalamic and cortical axons in vitro, and investigated the influence of this antibody on axonal growth in the chicken retina in vivo. Immunopurification, peptide sequencing, and biochemical character...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9970
更新日期：2001-01-01 00:00:00
abstract：:One way to better understand the molecular mechanisms involved in the construction of a nervous system is to identify the downstream effectors of major regulatory proteins. We previously showed that Engrailed (EN) and Gooseberry-Neuro (GsbN) transcription factors act in partnership to drive the formation of posterior ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.10.018
更新日期：2017-12-15 00:00:00
abstract：:Wnt signaling pathways have been demonstrated to play important roles in controlling tissue patterning and cell proliferation. In the gastrointestinal tract, mutations that lead to activation of the canonical Wnt pathway through beta-catenin result in familial and sporadic colon cancers. The downstream transcription f...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00185-4
更新日期：2003-07-15 00:00:00
abstract：:The transition from maternal to zygotic gene expression in the mouse occurs in the 2-cell embryo. Previous studies in which DNA was injected into 2-cell embryos revealed that transcription promoters and origins of DNA replication are strongly repressed in cleavage stage embryos unless linked to an embryo-responsive en...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1160
更新日期：1995-06-01 00:00:00
abstract：:Closure of the cranial neural tube depends on normal development of the head mesenchyme. Homozygous-mutant embryos for the ENU-induced open mind (opm) mutation exhibit exencephaly associated with defects in head mesenchyme development and dorsal-lateral hinge point formation. The head mesenchyme in opm mutant embryos ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.03.018
更新日期：2007-06-01 00:00:00
abstract：:A gene regulatory network (GRN) controls the process by which the endomesoderm of the sea urchin embryo is specified. In this GRN, the program of gene expression unique to the skeletogenic micromere lineage is set in train by activation of the pmar1 gene. Through a double repression system, this gene is responsible fo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.07.008
更新日期：2004-10-15 00:00:00
abstract：:The Drosophila Suppressor of Hairy wing [Su(Hw)] insulator protein has an essential role in the development of the female germline. Here we investigate the function of Su(Hw) in the ovary. We show that Su(Hw) is universally expressed in somatic cells, while germ cell expression is dynamic. Robust levels accumulate in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.666
更新日期：2011-08-15 00:00:00
abstract：:We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90147-b
更新日期：1990-11-01 00:00:00
abstract：:Temporal and spatial aspects of floral determination in seedling terminal buds of the qualitative short-day plant Pharbitis nil were examined using a grafting assay. Floral determination in the terminal buds of 6-day-old P. nil seedlings is rapid; by 9 hr after the end of a 14-hr inductive dark period more than 50% of...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90268-n
更新日期：1990-02-01 00:00:00
abstract：:NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.02.013
更新日期：2008-05-15 00:00:00
abstract：:Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.05.006
更新日期：2020-08-01 00:00:00
