Abstract:
:We recently discovered that in rat spermatids, kinesin light chain KLC3 can associate with outer dense fibers, major sperm tail components, and accumulates in the sperm midpiece. Here, we show that mitochondria isolated from rat-elongating spermatids have bound KLC3. Immunoelectron microscopy indicates that the association of KLC3 with mitochondria coincides with the stage in spermatogenesis when mitochondria move from the plasma membrane to the developing midpiece. KLC3 is able to bind in vitro to mitochondria from spermatids as well as somatic cells employing a conserved kinesin light chain motif, the tetratrico-peptide repeats. Expression of KLC3 in fibroblasts results in formation of large KLC3 clusters close to the nucleus, which also contain mitochondria: no other organelles were present in these clusters. Mitochondria are not present in KLC3 clusters after deletion of KLC3's tetratrico-peptide repeats. Our results indicate that the rat spermatid kinesin light chain KLC3 can associate with mitochondria.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Zhang Y,Oko R,van der Hoorn FAdoi
10.1016/j.ydbio.2004.07.014subject
Has Abstractpub_date
2004-11-01 00:00:00pages
23-33issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(04)00488-9journal_volume
275pub_type
杂志文章abstract::During early heart development the expression pattern of N-cadherin, a calcium-dependent cell adhesion molecule, suggests its involvement in morphoregulation and the stabilization of cardiomyocyte differentiation. N-cadherin's adhesive activity is dependent upon its interaction with the intracellular catenins. An asso...
journal_title:Developmental biology
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doi:10.1006/dbio.1997.8570
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abstract::The polypeptide sequence predicted from the Pem oncofetal gene cDNA contains a homeodomain most closely related to the paired class. Pem, paired class member orthodenticle, and the bicoid maternal gene product homeodomains all have lysine residues at a recognition helix position implicated in DNA-binding specificity. ...
journal_title:Developmental biology
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journal_title:Developmental biology
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更新日期:2007-02-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0122
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章,评审
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更新日期:2019-04-15 00:00:00
abstract::Cleft palate is one of the most common craniofacial congenital defects in humans. It is associated with multiple genetic and environmental risk factors, including mutations in the genes encoding signaling molecules in the sonic hedgehog (Shh) pathway, which are risk factors for cleft palate in both humans and mice. Ho...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.07.003
更新日期:2018-09-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.04.004
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2020-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
abstract::Recent studies with amoebae of the Myxomycetes Didymium iridis and Physarum polycephalum reveal that competence to undergo sexual fusion or to differentiate to plasmodia apogamically can be induced by a diffusible factor. Suspension cultures of Didymium amoebae have proven to be a good source of the inducer activity. ...
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doi:10.1016/0012-1606(84)90337-3
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journal_title:Developmental biology
pub_type: 杂志文章
doi:
更新日期:1996-09-15 00:00:00
abstract::The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.10.006
更新日期:2011-12-15 00:00:00
abstract::Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth fac...
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doi:10.1016/0012-1606(88)90161-3
更新日期:1988-09-01 00:00:00
abstract::The iris sphincter muscle consists of striated muscle fibers in the adult chicken. The ontogenetic development of this muscle has been studied by immunocytochemistry, from Embryonic Day 7 to the time of hatching. The time course of expression of specific markers of either smooth or striated muscle, i.e., smooth and sk...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1254
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abstract::To identify guanine nucleotide binding proteins (G-proteins) in sea urchin eggs and to investigate their role in signal transduction at fertilization, we used cholera toxin (CTX) and pertussis toxin (PTX), which catalyze the specific ADP-ribosylation of G-proteins. Cell surface complex, consisting of plasma membranes ...
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journal_title:Developmental biology
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