Abstract：
:Dorsoventral specification of the zebrafish gastrula is governed by the functions of the dorsal shield, a region of the embryo functionally analogous to the amphibian Spemann organizer. We report that the bozozok locus encodes the transcription factor nieuwkoid/dharma, a homeobox gene with non-cell-autonomous organizer-inducing activity. The nieuwkoid/dharma gene is expressed prior to the onset of gastrulation in a restricted region of an extraembryonic tissue, the yolk syncytial layer, that directly underlies the presumptive organizer cells. A single base-pair substitution in the nieuwkoid/dharma gene results in a premature stop codon in boz(m168) mutants, leading to the generation of a truncated protein product which lacks the homeodomain and fails to induce a functional organizer in misexpression assays. Embryos homozygous for the boz(m168) mutation exhibit impaired dorsal shield specification often leading to the loss of shield derivatives, such as prechordal plate in the anterior and notochord in the posterior, along the entire anteroposterior axis. Furthermore, boz homozygotes feature a loss of neural fates anterior to the midbrain/hindbrain boundary. Characterization of homozygous mutant embryos using molecular markers indicates that the boz ventralized phenotype may be due, in part, to the derepression of a secreted antagonizer of dorsal fates, zbmp2b, on the dorsal side of the embryo prior to the onset of gastrulation. Furthermore, ectopic expression of nieuwkoid/dharma RNA is sufficient to lead to the down regulation of zbmp2b expression in the pregastrula. Based on these results, we propose that gastrula organizer specification requires the Nieuwkoop center-like activity mediated by the nieuwkoid/dharma/bozozok homeobox gene and that this activity reveals the role of a much earlier than previously suspected inhibition of ventral determinants prior to dorsal shield formation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Koos DS,Ho RKdoi
10.1006/dbio.1999.9479subject
Has Abstractpub_date
1999-11-15 00:00:00pages
190-207issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(99)99479-4journal_volume
215pub_type
杂志文章abstract：:Nerve and muscle development was studied in paralysé mutant mice. The mutant phenotype is first recognizable 6-7 days after birth (PN 6-PN 7) as cessation of muscle growth and weakness and incoordination of movement. Mutant animals die between 2 and 3 weeks of age. Muscle fibers from paralysé mutants had a unimodal di...
journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:Climate change is multi-faceted, and includes changing concentrations of greenhouse gases in the atmosphere, rising temperatures, changes in precipitation patterns, and increasing frequency of extreme weather events. Here, we focus on the effects of rising atmospheric CO2 concentrations, rising temperature, and drough...
journal_title：Developmental biology
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abstract：:The kidney of the Gpc3-/ mouse, a novel model of human renal dysplasia, is characterized by selective degeneration of medullary collecting ducts preceded by enhanced cell proliferation and overgrowth during branching morphogenesis. Here, we identify cellular and molecular mechanisms underlying this renal dysplasia. Gl...
journal_title：Developmental biology
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abstract：:The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing method. This method has been used in a variety of organisms and cell types over the past several years. However, using this technology for generating gene-edited animals involves a number of obstacles. One such obstacle is mosaicism,...
journal_title：Developmental biology
pub_type： 杂志文章,评审
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abstract：:Expression of constitutively active Src protein tyrosine kinase in Xenopus oocytes has been shown to accelerate oocyte maturation suggesting that Src may be involved in meiotic progression. However, meiotic regulation of endogenous Src kinase in oocytes has not been investigated in detail. To address this problem, we ...
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2007-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8854
更新日期：1998-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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abstract：:Kidney development is a complex, little understood process based on inductive interactions and intricate epithelial and mesenchymal morphogenesis. Here, we report the use of subtractive hybridization to clone cDNAs expressed in early nephrogenesis. cDNA made from E14.5 mouse kidney was hybridized with adult mouse live...
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pub_type： 杂志文章
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