Abstract：
:Bone morphogenetic protein (BMP) signalling is key to many developmental processes, including early regionalisation of the ectoderm. The neural crest is induced here by a combination of BMP and Wnt signals from nearby tissues with many secreted factors contributing to its initial specification at the neural plate border. Gremlin 1 (Grem1) is a secreted BMP antagonist expressed in the neural crest in Xenopus laevis but its function here is unknown. As well as binding BMPs, Grem1 has been shown to interact with heparan sulfate proteoglycans (HSPGs), a family of cell surface macromolecules that regulate a diverse array of signalling molecules by affecting their availability and mode of action. This study describes the impact of HSPGs on the function of Grem1 in neural crest induction. It shows for the first time that Grem1 is required for neural crest development in a two-step process comprising an early HSPG-independent, followed by a late HSPG-dependent phase.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Pegge J,Tatsinkam AJ,Rider CC,Bell Edoi
10.1016/j.ydbio.2019.12.015subject
Has Abstractpub_date
2020-04-15 00:00:00pages
108-114issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(19)30171-Xjournal_volume
460pub_type
杂志文章abstract：:Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.01.010
更新日期：2017-02-15 00:00:00
abstract：:Expression of cardiac troponin I (TnIcardiac) and slow skeletal troponin I (TnIslow) genes was analyzed at the mRNA and protein level in the developing rat heart. TnIslow mRNA was detectable by in situ hybridization in the embryonic cardiac tube as early as the 13-somite stage (Embryonic Day 10). In contrast, TnIcardi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1074
更新日期：1993-03-01 00:00:00
abstract：:The ectoderm of early Xenopus gastrula is competent to become induced to neural tissue, but dorsal ectoderm is more neural competent than ventral ectoderm. It is a tenable, but as yet untested possibility that the higher neural competence of dorsal gastrula ectoderm is dependent on the presence of the dorsal mesoderm....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90168-g
更新日期：1992-07-01 00:00:00
abstract：:GDF11, a new member of the TGF-beta gene superfamily, regulates anterior/posterior patterning in the axial skeleton during mouse embryogenesis. Gdf11 null mice display skeletal abnormalities that appear to represent anterior homeotic transformations of vertebrae consistent with high levels of Gdf11 expression in...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9981
更新日期：2001-01-15 00:00:00
abstract：:This study reports a morphological, skeletal, and molecular characterization of the supernumerary limbs induced by systemic administration of all-trans retinoic acid to egg-cylinder stage mouse embryos. As initially described by Rutledge et al. (Proc. Natl. Acad. Sci. USA 91, 5436, 1994), we have found that oral admin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0126
更新日期：1996-06-15 00:00:00
abstract：:We have investigated the sequential changes of acetylcholine receptor (AChR) distribution on identified Xenopus laevis muscle cells in culture before and after innervation. AChRs on muscle cells were stained with tetramethylrhodamine-conjugated alpha-bungarotoxin and the distribution of AChR clusters was examined on a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90006-x
更新日期：1984-05-01 00:00:00
abstract：:Replication-dependent histone genes in the mouse and Xenopus share a common regulatory element within the protein-encoding sequence called the CRAS alpha element (coding region activating sequence alpha) which has been shown to mediate normal expression in vivo and to interact with nuclear factors in vitro in a cell c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8459
更新日期：1997-02-01 00:00:00
abstract：:The eukaryotic initiation translation factor eIF6 is a highly conserved, essential protein implicated in translation. eIF6 is regulated in vivo by extracellular signals, such as IGF signaling (for a review see Miluzio et al., 2009). In Xenopus, eif6 over-expression causes a delay in eye development (De Marco et al., 2...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.04.017
更新日期：2017-07-01 00:00:00
abstract：:In studies initially focused on roles of nonmuscle myosin IIA (NMIIA) in the developing mouse epidermis, we have discovered that a previously described cytokeratin 5 (K5)-Cre gene construct is expressed in early embryo development. Mice carrying floxed alleles of the nonmuscle myosin II heavy chain gene (NMHC IIA(flox...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.017
更新日期：2013-10-01 00:00:00
abstract：:Monoclonal antibodies against the surface of embryonic osteogenic cells (SB-1, SB-2, SB-3, and SB-5) have been used to characterize the sequence of transitions involved in the osteogenic cell lineage. In the present study, immunohistochemical analyses of the expression of osteogenic cell surface antigens in organ cult...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90388-y
更新日期：1990-10-01 00:00:00
abstract：:Characterization of the molecular pathways controlling differentiation and proliferation in mammalian hair follicles is central to our understanding of the regulation of normal hair growth, the basis of hereditary hair loss diseases, and the origin of follicle-based tumors. We demonstrate that the proto-oncogene Wnt3,...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.9140
更新日期：1999-03-01 00:00:00
abstract：:During early vertebrate development, ANF homeobox genes are expressed in the prospective forebrain. Their regulation is essential for correct morphogenesis and function of the prosencephalon. We identified a 1-kb fragment upstream of the chicken GANF gene sufficient to drive lacZ expression in the endogenous expressio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.044
更新日期：2004-05-15 00:00:00
abstract：:We have studied the effect of saline and acid extracts of chick brain on the total number of acetylcholine (ACh) receptors and the number of receptor clusters in cultured chick muscle cells. Myotubes in 7-day cultures responded more rapidly to brain extract than did myotubes in 4-day cultures, so the older cells were ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90039-8
更新日期：1983-02-01 00:00:00
abstract：:Chondrogenesis of mesenchymal cells from the frontonasal mass, mandibles and maxillae of stage-24 chick embryos has been investigated in micromass (high-density) cultures. Distinct differences in the amount and pattern of cartilage differentiation are found. In cultures of frontonasal mass cells, a central sheet of ca...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90349-0
更新日期：1986-09-01 00:00:00
abstract：:The Smcp mRNA encoding the sperm mitochondria-associated cysteine-rich protein is translationally repressed in round spermatids and translationally active in elongated spermatids. The patterns of transcription and translation of fusions of the Smcp promoter, the green fluorescent protein coding region (Gfp) and variou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.468
更新日期：2006-09-01 00:00:00
abstract：:Neurons of the dorsal hindbrain and spinal cord are central in receiving, processing and relaying sensory perception and participate in the coordination of sensory-motor output. Numerous cellular and molecular mechanisms that underlie neuronal development in both regions of the nervous system are shared. We discuss he...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2016.10.008
更新日期：2017-12-01 00:00:00
abstract：:We have isolated a cDNA of the DEAD protein family from a Xenopus ovary cDNA library. Northern hybridization revealed that the mRNA corresponding to the cDNA [XVLG1 (Xenopus vasa-like gene)] was specifically expressed in adult testis and ovary. The deduced amino acid sequence of XVLG1 was relatively homologous to that...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1093
更新日期：1994-04-01 00:00:00
abstract：:Morphogenesis of the vertebrate head relies on proper dorsal-ventral (D-V) patterning of neural crest cells (NCC) within the pharyngeal arches. Endothelin-1 (Edn1)-induced signaling through the endothelin-A receptor (Ednra) is crucial for cranial NCC patterning within the mandibular portion of the first pharyngeal arc...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.08.003
更新日期：2012-11-01 00:00:00
abstract：:The vertebrate cranial base is a complex structure composed of bone, cartilage and other connective tissues underlying the brain; it is intimately connected with development of the face and cranial vault. Despite its central importance in craniofacial development, morphogenesis and tissue origins of the cranial base h...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.07.016
更新日期：2008-10-01 00:00:00
abstract：:DSL (Delta, Serrate, Lag-2) ligands activate Notch signaling and thereby regulate the differentiation of many different cell types during development. We have isolated a novel Serrate-like gene, Jagged2, whose amino acid sequence and expression pattern during rat embryogenesis suggest that it functions as a ligand for...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0310
更新日期：1996-11-25 00:00:00
abstract：:FgfrL1, which interacts with Fgf ligands and heparin, is a member of the fibroblast growth factor receptor (Fgfr) family. FgfrL1-deficient mice show two significant alterations when compared to wildtype mice: They die at birth due to a malformed diaphragm and they lack metanephric kidneys. Utilizing gene arrays, qPCR ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.08.016
更新日期：2014-10-15 00:00:00
abstract：:Previous work has established that dendritic spines, sites of excitatory input in CNS neurons, can be highly dynamic, in later development as well as in mature brain. Although spine motility has been proposed to facilitate the formation of new synaptic contacts, we have reported that spines continue to be dynamic even...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.09.028
更新日期：2005-01-15 00:00:00
abstract：:Procedures are described for removal of the vitelline membrane from gently fixed Drosophila embryos en masse. The resulting embryos retain excellent structural integrity and are now suitable for a variety of immunocytochemical and biochemical characterizations. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90275-0
更新日期：1983-09-01 00:00:00
abstract：:To analyze the molecular mechanism of the limb pattern formation, we have tried to make monoclonal antibodies against antigens from chick limb buds. We obtained one antibody named AV-1 which recognized a specific region of chick limb buds. AV-1 reacted with the distal portion of the anteroventral mesoderm of only deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90337-4
更新日期：1986-10-01 00:00:00
abstract：:Three myogenic clonal cell lines were isolated from C3H 10T1/2 C18 cells (10T1/2) treated with 5-azacytidine (5-aza-CR). These lines reproducibly underwent fusion at confluence into functional myotubes capable of contracting in response to acetylcholine. The degree of fusion could be increased two- to threefold if the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90302-7
更新日期：1986-10-01 00:00:00
abstract：:A protein complex consisting of Mago Nashi and Tsunagi/Y14 is required to establish the major body axes and for the localization of primordial germ cell determinants during Drosophila melanogaster oogenesis. The Mago Nashi:Tsunagi/Y14 heterodimer also serves as the core of the exon junction complex (EJC), a multiprote...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.007
更新日期：2007-08-15 00:00:00
abstract：:Insulin-producing beta-cells are present as single cells or in small clusters distributed throughout the pancreas of the Xenopus laevis tadpole. During metamorphic climax when the exocrine pancreas dedifferentiates to progenitor cells, the beta-cells undergo two changes. Insulin mRNA is down regulated at the beginning...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.038
更新日期：2009-04-15 00:00:00
abstract：:Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.08.002
更新日期：2020-10-01 00:00:00
abstract：:Zygotic Wnt signaling has been shown to be involved in dorsoventral mesodermal patterning in Xenopus embryos, but how it regulates different myogenic gene expression in the lateral mesodermal domains is not clear. Here, we use transient exposure of embryos or explants to lithium, which mimics Wnt/beta-catenin signalin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0633
更新日期：2002-05-01 00:00:00
abstract：:The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90089-2
更新日期：1985-08-01 00:00:00
