Abstract:
:In order to disclose the mechanism that regulate synapse development we compared the distributions of agrin, acetylcholine receptors (AChR) and a basal lamina heparan sulfate proteoglycan (HSPG) in sections and cultures prepared from Xenopus laevis and Ambystoma mexicanum embryos. While agrin, AChR and HSPG may accumulate almost synchronously at synapses in vivo, agrin deposition usually lagged well behind the other synaptic markers during development in culture, and was not detectable at many differentiated junctions. Agrin deposition at nerve-muscle contacts in culture also appeared to require the presence of other synaptic components. A similarly variable deposition occurred on noninnervated myocytes, where agrin again collected near sites of HSPG and AChR accumulation on some cells. Profuse agrin accretion occurred consistently, however, within the extracellular matrices of surrounding epithelial cells derived from both myotomes and neural tubes. In cocultures of Ambystoma neurons and Xenopus myocytes Ambystoma agrin collected at some chimeric neuromuscular junctions, but also accumulated on noninnervated myocytes and in the extracellular matrices of salamander neuroendothelial cells. Based upon these observations we conclude that (a) focal agrin deposition is not required for synaptic differentiation on Xenopus myocytes and (b) agrin may be one of several muscle basal lamina components that stem mainly from the secreted products of nearby epithelial cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Anderson MJ,Shi ZQ,Grawel R,Zackson SLdoi
10.1006/dbio.1995.1191subject
Has Abstractpub_date
1995-07-01 00:00:00pages
1-20issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(85)71191-8journal_volume
170pub_type
杂志文章abstract::Syndecan-3 is one of four identified members of a family of transmembrane proteoglycans (the syndecans) that possess highly similar cytoplasmic and transmembrane domains and may function as extracellular matrix receptors and/or low affinity receptors for signaling molecules such as FGF. We previously reported the clon...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1093
更新日期:1995-04-01 00:00:00
abstract::Neurogenin (Ngn) 1 and Ngn2 encode basic-helix-loop-helix transcription factors expressed in the developing neocortex. Like other proneural genes, Ngns participate in the specification of neural fates and neuronal identities, but downstream effectors remain poorly defined. We set out to identify Ngn2 effectors in the ...
journal_title:Developmental biology
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更新日期:2004-09-15 00:00:00
abstract::Inverse gradients of transcriptional repressors antagonize the transcriptional effector response to morphogens. However, the role of such inverse regulation might not manifest solely from lack of repressors. Sonic hedgehog (Shh) patterns the forebrain by being expressed ventrally; however, absence of antagonizing Gli3...
journal_title:Developmental biology
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更新日期:2019-06-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90445-9
更新日期:1991-07-01 00:00:00
abstract::An important developmental question concerns whether neurotransmitter phenotype is an inherent property of neurons or is influenced by target tissues. This issue can be addressed in the avian ciliary ganglion (CG) which contains two cholinergic populations, ciliary and choroid neurons, that differentially express the ...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:1998-10-15 00:00:00
abstract::Collapsin-1 is a secreted glycoprotein that inhibits the extension of specific growth cones in vitro. It has been hypothesized to serve as a repulsive guidance cue for extending growth cones in vivo. Here we report the distribution of collapsin-1 message as demonstrated by in situ hybridization using digoxigenin-label...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0016
更新日期:1996-01-10 00:00:00
abstract::Using lineage tracers, we recently showed dorsal and ventral ectodermal compartments along the sides of the body in chick embryos. The compartments are formed both in presumptive limb-forming regions where they position the apical ridge and also in presumptive interlimb (flank). Here we show, using a novel technique c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9659
更新日期:2000-06-15 00:00:00
abstract::A monoclonal antibody, Mab 8A2, that recognizes a novel set of gangliosides was produced by immunizing a mouse with Embryonic Day 14 chick optic nerve. Immunohistochemical studies of the developing chick retina revealed a complex pattern of Mab 8A2 immunoreactivity. Initially, staining is concentrated in the optic fib...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90221-n
更新日期:1991-05-01 00:00:00
abstract::Smad4 is defined as the common-mediator Smad (co-Smad) required for transducing signals for all TGF-beta superfamily members. This paper describes two Smad4s in Xenopus: XSmad4alpha, which is probably the Xenopus orthologue of human Smad4, and a distinct family member, XSmad4beta, which differs primarily at the extrem...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9430
更新日期:1999-10-15 00:00:00
abstract::Differences are demonstrated in the chondrogenic potential of cells derived from the distal and proximal halves of chick wing buds from as early as stage 23, prior to the appearance of overt cartilage differentiation. In high cell density cultures, cells obtained from the distal portions of stage 23 or 24 limb buds ar...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90063-5
更新日期:1983-05-01 00:00:00
abstract::Enhancers are flexible in terms of their location and orientation relative to the genes they regulate. However, little is known about whether the flexibility can be applied in every combination of enhancers and genes. Enhancer detection with transposable elements is a powerful method to identify enhancers in the genom...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.12.012
更新日期:2013-03-01 00:00:00
abstract::Rex1 (Zfp42), GeneID 132625, is a gene whose expression is closely associated with pluripotency/multipotency in both mouse and human embryonic stem cells. To study the function of the murine Rex1 gene in vivo, we have used cre/lox technology to create Rex1(floxed) mice and mice deficient in Rex1 gene function. Rex1(-/...
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pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.664
更新日期:2011-08-15 00:00:00
abstract::Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0718
更新日期:2002-08-15 00:00:00
abstract::Nematodes provide a useful experimental system with which to investigate the evolution of development at the cellular, genetic, and molecular levels. Building on an understanding of vulval development in Caenorhabditis elegans, analysis of vulval development has been extended to a number of other species in three fami...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1006/dbio.1996.0035
更新日期:1996-02-01 00:00:00
abstract::One rapid response of starfish oocytes to the maturation-inducing hormone, 1-methyladenine (1-MA), is the formation of transient actin-filled spikes on the cell surface. The presence and distribution of G- and F-actin and several actin-associated proteins were examined in cortices isolated from oocytes before, during,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90140-4
更新日期:1984-02-01 00:00:00
abstract::During the last stages of fetal life, the immature epithelial cells of the rat lung alveolus develop the properties of mature type 2 cells. Adult type 2 cells rapidly lose these same properties when isolated and maintained in cell culture. We have examined the synthesis of cytokeratin proteins by adult type 2 cells as...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90396-x
更新日期:1988-10-01 00:00:00
abstract::Primitive erythropoiesis is regulated in a non cell-autonomous fashion across evolution from frogs to mammals. In Xenopus laevis, signals from the overlying ectoderm are required to induce the mesoderm to adopt an erythroid fate. Previous studies in our lab identified the transcription factor GATA2 as a key regulator ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.08.012
更新日期:2015-11-01 00:00:00
abstract::Antibodies to nonerythroid alpha spectrin (p 230) were used to study the distribution of this polypeptide in mouse germ cells, zygote, and early embryonic cells. In the primordial germ cells, fetal oocytes, and spermatogonia, spectrin was found predominantly in the form of a narrow condensed subplasmalemmal band, as i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90389-1
更新日期:1986-03-01 00:00:00
abstract::The midbrain-hindbrain boundary (MHB) acts as a local organizer in the development of the CNS in vertebrates. Previously, we identified an MHB-specific bHLH-WRPW transcriptional repressor gene, Xenopus Hes-related 1 (XHR1), which is initially expressed in the presumptive MHB (pre-MHB) region at the early gastrula stag...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.04.020
更新日期:2005-07-01 00:00:00
abstract::Using two different models of pregnancy failure in mice, we show in this communication that preimplantation embryonic development can be controlled, in vivo, with cytokines. In one model, described by us previously, CSF-1 is shown to block gestations in plugged females due to its induction of defective early developme...
journal_title:Developmental biology
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abstract::The uterus undergoes a series of dramatic changes in response to an implanting conceptus that, in some mammalian species, includes differentiation of the endometrial stroma into decidual tissue. This process, called decidualization, can be induced artificially in rodents indicating that the conceptus may not be essent...
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更新日期:2006-06-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0717
更新日期:2002-08-01 00:00:00
abstract::Epithelial-mesenchymal interactions are of major importance during development to direct correct differentiation and morphogenesis of embryonic tissues. One subset of lateral mesoderm-derived mesenchymal cells will form the smooth muscle (SM) layer of the primary epithelial lining of hollow internal organs. It has bee...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1207
更新日期:1994-08-01 00:00:00
abstract::Myogenic precursors in adult skeletal muscle (satellite cells) are mitotically quiescent but can proliferate in response to a variety of stresses including muscle injury. To gain further understanding of adult myoblasts, we analyzed myogenesis of satellite cells on intact fibers isolated from adult rat muscle. In this...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1226
更新日期:1994-08-01 00:00:00
abstract::Neurons and glia are produced in stereotyped patterns after neuroblast cell division during development of the Drosophila central nervous system. The first cell division of thoracic neuroblast 6-4 (NB6-4T) is asymmetric, giving rise to a glial precursor cell and a neuronal precursor cell. In contrast, abdominal NB6-4 ...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2000-06-15 00:00:00
abstract::Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.08.002
更新日期:2020-10-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90220-5
更新日期:1988-02-01 00:00:00
abstract::Vulval epithelial tubes invaginate through concerted cell migration, ring formation, stacking of rings and intra-ring cell fusion in the nematodes Caenorhabditis elegans, Oscheius tipulae and Pristionchus pacificus. The number of rings forming the invaginations is invariantly seven, six, and eight, respectively. We hy...
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
abstract::In C. elegans the 4-cell stage blastomere EMS is an endomesodermal precursor. Its anterior daughter, MS, makes primarily mesodermal cells, while its posterior daughter E generates the entire intestine. The gene regulatory network underlying specification of MS and E has been the subject of study for more than 15 years...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.042
更新日期:2010-04-15 00:00:00
abstract::It has been posited that animal development evolved from pre-existing mechanisms for regulating cell differentiation in the single celled and colonial ancestors of animals. Although the progenitors of animals cannot be studied directly, insights into their cell biology may be gleaned from comparisons between animals a...
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pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00