Abstract：
:The CRISPR/Cas9 system is a rapid, simple, and often extremely efficient gene editing method. This method has been used in a variety of organisms and cell types over the past several years. However, using this technology for generating gene-edited animals involves a number of obstacles. One such obstacle is mosaicism, which is common in founder animals. This is especially the case when the CRISPR/Cas9 system is used in embryos. Here we review the pros and cons of mosaic mutations of gene-edited animals caused by using the CRISPR/Cas9 system in embryos. Furthermore, we will discuss the mechanisms underlying mosaic mutations resulting from the CRISPR/Cas9 system, as well as the possible strategies for reducing mosaicism. By developing ways to overcome mosaic mutations when using CRISPR/Cas9, genotyping for germline gene disruptions should become more reliable. This achievement will pave the way for using the CRISPR technology in the research and clinical applications where mosaicism is an issue.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Mehravar M,Shirazi A,Nazari M,Banan Mdoi
10.1016/j.ydbio.2018.10.008subject
Has Abstractpub_date
2019-01-15 00:00:00pages
156-162issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(18)30251-3journal_volume
445pub_type
杂志文章,评审abstract：:Chick sensory neurons grow to their correct targets in the hindlimb from the outset during normal development and following various experimental manipulations. This may result not because sensory neurons respond to specific limb-derived cues, but because they interact in some way with motoneurons which are responsive ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90023-0
更新日期：1986-12-01 00:00:00
abstract：:Cell adhesion molecules (CAMs) perform numerous functions during neural development. An individual CAM can play different roles during each stage of neuronal differentiation; however, little is known about how such functional switching is accomplished. Here we show that Drosophila N-cadherin (CadN) is required at mult...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2012.04.006
更新日期：2012-06-15 00:00:00
abstract：:Human MusTRD1alpha1 was isolated as a result of its ability to bind a critical element within the Troponin I slow upstream enhancer (TnIslow USE) and was predicted to be a regulator of slow fiber-specific genes. To test this hypothesis in vivo, we generated transgenic mice expressing hMusTRD1alpha1 in skeletal muscle....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.01.019
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0850
更新日期：2002-12-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1024
更新日期：1995-01-01 00:00:00
abstract：:Immature, Stage VI oocytes of Xenopus laevis fail to activate (i.e., to propagate a cortical reaction and elevate a fertilization envelope) when pricked or exposed to A23187. We determined the times during maturation when immature oocytes treated with progesterone in vitro developed the capacity to respond to pricking...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90177-5
更新日期：1984-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00014-9
更新日期：2003-04-01 00:00:00
abstract：:Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.01.040
更新日期：2007-05-01 00:00:00
abstract：:Syndecan, a cell surface proteoglycan, binds multiple extracellular ligands, and is developmentally regulated in epithelial and mesenchymal tissues. The branching morphogenesis of embryonic lung is dependent on epithelial-mesenchymal interactions and, based on studies with inhibitors, on proteoglycan synthesis. To ass...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90286-c
更新日期：1991-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90339-9
更新日期：1988-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.019
更新日期：2008-07-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.09.012
更新日期：2016-11-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.05.666
更新日期：2011-08-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1015
更新日期：1995-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.03.012
更新日期：2010-06-15 00:00:00
abstract：:We have performed a detailed analysis of the expression pattern of the three gnathostome Otx classes in order to gain new insights into their functional evolution. Expression patterns were examined in the developing eye of a chondrichthyan, the dogfish, and an amniote, the chick, and compared with the capacity of para...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.11.019
更新日期：2005-02-15 00:00:00
abstract：:A cDNA library was used to measure changes in many individual mRNAs during muscle differentiation in culture. A library of 1000 clones was constructed from total myofiber poly(A) RNA. About 23% of these clones gave a detectable colony hybridization signal using end-labeled myofiber mRNA, the remainder containing muscl...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90049-0
更新日期：1983-02-01 00:00:00
abstract：:Monoclonal antibodies (mAbs), electrophoresis, immunoblotting, and immunohistochemistry were used to determine the molecular properties of cardiac myosin heavy chain (MHC) isoforms and the regions of the developing chicken heart in which they were expressed. Adult atria expressed three electrophoretically distinct MHC...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90324-7
更新日期：1988-06-01 00:00:00
abstract：:In the ascidian Ciona intestinalis, oral siphon amputation activates adult stem cell niches in the branchial sac to divide and dispatch migratory progenitor cells to a regeneration blastema at the site of injury. This study shows that progenitor cells derived from branchial sac stem cell niches have roles in homeostas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.005
更新日期：2019-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2003.10.005
更新日期：2004-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1995-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2006-07-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2016-02-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90378-8
更新日期：1988-10-01 00:00:00
abstract：:The facial primordia in the chick embryo begin as rounded swellings that surround the primitive mouth and these grow out to form the beak. The control of proximodistal outgrowth is not well understood but may involve similar mechanisms to the limb bud. In order to test this hypothesis, combinations were made between e...
journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1998-11-01 00:00:00
abstract：:In this study, we demonstrate the developmental activation, in the zebrafish embryo, of a surveillance mechanism which triggers apoptosis to remove damaged cells. We determine the time course of activation of this mechanism by exposing embryos to camptothecin, an agent which specifically inhibits topoisomerase I withi...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1999-05-15 00:00:00
abstract：:Mf1, which encodes a winged-helix/forkhead transcription factor, is the murine homolog of human FKHL7, mutated in individuals with autosomal dominant inherited dysgenesis of the anterior segment of the eye (Axenfeld-Reiger anomaly). Mouse embryos homozygous for null mutations in Mf1 (Mf1(lacZ) and Mf1(ch)) show severe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9314
更新日期：1999-07-15 00:00:00
