Abstract：
:Vascular smooth muscle of the head derives from neural crest, but developmental mechanisms and early transcriptional drivers of the vSMC lineage are not well characterized. We find that in early development, the transcription factor foxc1b is expressed in mesenchymal cells that associate with the vascular endothelium. Using timelapse imaging, we observe that foxc1b expressing mesenchymal cells differentiate into acta2 expressing vascular mural cells. We show that in zebrafish, while foxc1b is co-expressed in acta2 positive smooth muscle cells that associate with large diameter vessels, it is not co-expressed in capillaries where pdgfrβ positive pericytes are located. In addition to being an early marker of the lineage, foxc1 is essential for vSMC differentiation; we find that foxc1 loss of function mutants have defective vSMC differentiation and that early genetic ablation of foxc1b or acta2 expressing populations blocks vSMC differentiation. Furthermore, foxc1 is expressed upstream of acta2 and is required for acta2 expression in vSMCs. Using RNA-Seq we determine an enriched intersectional gene expression profile using dual expression of foxc1b and acta2 to identify novel vSMC markers. Taken together, our data suggests that foxc1 is a marker of vSMCs and plays a critical functional role in promoting their differentiation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Whitesell TR,Chrystal PW,Ryu JR,Munsie N,Grosse A,French CR,Workentine ML,Li R,Zhu LJ,Waskiewicz A,Lehmann OJ,Lawson ND,Childs SJdoi
10.1016/j.ydbio.2019.06.005subject
Has Abstractpub_date
2019-09-01 00:00:00pages
34-47issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(19)30209-Xjournal_volume
453pub_type
杂志文章abstract：:To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1341
更新日期：1994-12-01 00:00:00
abstract：:Actin dynamics are necessary at multiple steps in the formation of multinucleated muscle cells. BAR domain proteins can regulate actin dynamics in several cell types, but have been little studied in skeletal muscle. Here, we identify novel functions for the N-BAR domain protein, Bridging integrator 3 (Bin3), during my...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.07.004
更新日期：2013-10-01 00:00:00
abstract：:The origin and development of mouse kidney vasculature were examined in chorioallantoic grafts of early kidney rudiments and of experimentally induced explants of separated metanephric mesenchymes. Whole kidney rudiments developed into advanced stages, expressed the segment-specific antigenic markers of tubules and th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90180-x
更新日期：1983-04-01 00:00:00
abstract：:Hair shafts are produced from stem cells located in the bulge. Our knowledge of the genetic pathways regulating cell fate acquisition in the immediate descendents of these stem cells, and fate maintenance in their committed progeny, is still incomplete. One pathway involved in fate maintenance within the hair matrix i...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.11.021
更新日期：2009-02-15 00:00:00
abstract：:We investigated the potential role of rostral-caudal and dorsal-ventral subdivisions of the early rostral brain by relating these subdivisions to the early patterning of neuron cell bodies and their axon projections. The earliest neurons were mapped using the lipophilic axon tracers diI and diO on embryos fixed on emb...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0008
更新日期：1996-01-10 00:00:00
abstract：:Expression of cardiac troponin I (TnIcardiac) and slow skeletal troponin I (TnIslow) genes was analyzed at the mRNA and protein level in the developing rat heart. TnIslow mRNA was detectable by in situ hybridization in the embryonic cardiac tube as early as the 13-somite stage (Embryonic Day 10). In contrast, TnIcardi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1074
更新日期：1993-03-01 00:00:00
abstract：:The tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.09.023
更新日期：2019-04-15 00:00:00
abstract：:A glycoprotein from the liver, which shares epitopes with chorion (egg envelope or zona pellucida) glycoproteins, is present only in the spawning female fish, Oryzias latipes, under natural conditions. This spawning female-specific (SF) substance is distinct from vitellogenin but closely resembles a major glycoprotein...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90301-1
更新日期：1989-05-01 00:00:00
abstract：:Exocytosis of cortical granules in mouse eggs is required to produce the zona pellucida block to polyspermy. In this study, we examined the role of microfilaments and microtubules in the regulation of cortical granule movement toward the cortex during oocyte maturation and anchoring of cortical granules in the cortex....
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8945
更新日期：1998-08-01 00:00:00
abstract：:Initial studies suggested that spatial organization of the putative polar body contractile ring was determined by the peripheral aster in Spisula [Biol. Bull. 205 (2003) 192]. Here we report detailed supporting observations, including testing of aster and ring function with inhibitors. The metaphase peripheral aster w...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.01.033
更新日期：2004-05-15 00:00:00
abstract：:The Hippo tumor suppressor pathway plays many fundamental cell biological roles during animal development. Two central players in controlling Hippo-dependent gene expression are the TEAD transcription factor Scalloped (Sd) and its transcriptional co-activator Yorkie (Yki). Hippo signaling phosphorylates Yki, thereby b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.06.022
更新日期：2019-11-01 00:00:00
abstract：:Fertilization results in the tyrosine phosphorylation of several egg proteins within minutes of sperm-egg binding and inhibitor studies have shown that tyrosine kinase activity is required for many aspects of egg activation. The present study demonstrates the presence of p59c-fyn kinase in the sea urchin egg and exami...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0073
更新日期：1996-03-15 00:00:00
abstract：:Xenopus laevis is among the few species that are capable of fully regenerating a lost lens de novo. This occurs upon removal of the lens, when secreted factors from the retina are permitted to reach the cornea epithelium and trigger it to form a new lens. Although many studies have investigated the retinal factors tha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2013.12.036
更新日期：2014-02-15 00:00:00
abstract：:Nociceptive neurons innervate the skin with complex dendritic arbors that respond to pain-evoking stimuli such as harsh mechanical force or extreme temperatures. Here we describe the structure and development of a model nociceptor, the PVD neuron of C. elegans, and identify transcription factors that control morphogen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.05.502
更新日期：2010-09-01 00:00:00
abstract：:The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90004-0
更新日期：1983-01-01 00:00:00
abstract：:A simple assay has been developed to measure cGMP-specific phosphodiesterase (cGPD) activity in crude soluble extracts of amoebae of Dictyostelium discoideum. When amoebae of different wild-type strains were starved on buffered agar, all strains exhibited an 8- to 12-fold increase in cGMP-specific hydrolyzing activity...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90026-5
更新日期：1984-05-01 00:00:00
abstract：:The N-ethyl-N-nitrosourea (ENU) ←forward genetic screen is a useful tool for the unbiased discovery of novel mechanisms regulating developmental processes. We recovered the dorothy mutation in such a screen designed to recover recessive mutations affecting craniofacial development in the mouse. Dorothy embryos die pre...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.10.039
更新日期：2020-02-15 00:00:00
abstract：:Large steady electrical currents traverse Drosophila follicles in vitro as well as permeabilized eggs. During the period of main follicle growth (stages 9-11), these currents enter the anterior or nurse cell end of the follicles. This inward current acts like a sodium ion influx with some calcium involvement. During t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90013-2
更新日期：1985-03-01 00:00:00
abstract：:CHARGE syndrome is caused by spontaneous loss-of-function mutations to the ATP-dependant chromatin remodeller chromodomain-helicase-DNA-binding protein 7 (CHD7). It is characterised by a distinct pattern of congenital anomalies, including cardiovascular malformations. Disruption to the neural crest lineage has previou...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.06.017
更新日期：2015-09-01 00:00:00
abstract：:Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.12.022
更新日期：2006-03-15 00:00:00
abstract：:The sizes of amino acid pools in growing Xenopus laevis oocytes have been measured. The total free amino acid content per oocyte increases nearly 25-fold during oocyte growth. Together, glutamic acid and aspartic acid account for approximately 59-75% of the total amino acid pool in Xenopus oocytes. On the other hand, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90480-5
更新日期：1987-11-01 00:00:00
abstract：:Epithelial planar cell polarity (PCP) allows epithelial cells to coordinate their development to that of the tissue in which they reside. The mechanisms that impart PCP as well as effectors that execute the polarizing instructions are being sought in many tissues. We report that the epidermal epithelium of Drosophila ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.04.454
更新日期：2006-09-15 00:00:00
abstract：:In mouse sex differentiation, SRY promotes Sertoli cell differentiation via SOX9 action, resulting in testis formation. SRY/SOX9 also initiates various testis-specific morphogenic events including glycogenesis in pre-Sertoli cells, suggesting the importance of glucose storage for certain SRY/SOX9-downstream events in ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.09.004
更新日期：2008-12-01 00:00:00
abstract：:A subset of macrophages that reside in adult tissues originate from the fetal yolk sac, while others derive from circulating monocytes. These ontologically different macrophage subsets have distinct roles in tissue injury responses, with the embryonic population overall having beneficial activity in cardiac repair. He...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.03.014
更新日期：2016-05-15 00:00:00
abstract：:A fundamental question in biology is how animal segmentation arose during evolution. One particular challenge is to clarify whether segmental ganglia of the nervous system evolved once, twice, or several times within the Bilateria. As close relatives of arthropods, Onychophora play an important role in this debate sin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.08.011
更新日期：2009-11-01 00:00:00
abstract：:Metamorphosis is a postembryonic developmental process that involves morphophysiological and behavioral changes, allowing organisms to adapt into a novel environment. In some amphibians, aquatic organisms undergo metamorphosis to adapt in a terrestrial environment. In this process, these organisms experience major cha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.08.002
更新日期：2020-10-01 00:00:00
abstract：:Glycosaminoglycans in the form of heparan sulfate proteoglycans (HSPG) and chondroitin sulfate proteoglycans (CSPG) are required for normal kidney organogenesis. The specific roles of HSPGs and CSPGs on ureteric bud (UB) branching morphogenesis are unclear, and past reports have obtained differing results. Here we emp...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.029
更新日期：2004-08-15 00:00:00
abstract：:Eph receptors play important roles in axon guidance at the midline. In the auditory system, growth of axons across the midline is an important determinant of auditory function. The avian cochlear nucleus, n. magnocellularis (NM), makes bilateral projections to its target, n. laminaris (NL). We examined the time course...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.03.010
更新日期：2006-07-01 00:00:00
abstract：:This investigation provides the first systematic determination of the cellular and molecular progression of vocal fold (VF) epithelium development in a murine model. We define five principal developmental events that constitute the progression from VF initiation in the embryonic anterior foregut tube to fully differen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.12.037
更新日期：2015-03-15 00:00:00
abstract：:We have characterized the antigen recognized by mab10, a monoclonal antibody that has been shown to modify outgrowth of thalamic and cortical axons in vitro, and investigated the influence of this antibody on axonal growth in the chicken retina in vivo. Immunopurification, peptide sequencing, and biochemical character...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9970
更新日期：2001-01-01 00:00:00
