Abstract:
:Cis-regulatory modules (CRMs) control spatiotemporal gene expression patterns in embryos. While measurement of quantitative CRM activities has become efficient, measurement of spatial CRM activities still relies on slow, one-by-one methods. To overcome this bottleneck, we have developed a high-throughput method that can simultaneously measure quantitative and spatial CRM activities. The new method builds profiles of quantitative CRM activities measured at single-embryo resolution in many mosaic embryos and uses these profiles as a 'fingerprint' of spatial patterns. We show in sea urchin embryos that the new method, Multiplex and Mosaic Observation of Spatial Information encoded in Cis-regulatory modules (MMOSAIC), can efficiently predict spatial activities of new CRMs and can detect spatial responses of CRMs to gene perturbations. We anticipate that MMOSAIC will facilitate systems-wide functional analyses of CRMs in embryos.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Guay CL,McQuade ST,Nam Jdoi
10.1016/j.ydbio.2017.01.010subject
Has Abstractpub_date
2017-02-15 00:00:00pages
92-104issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(16)30660-1journal_volume
422pub_type
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