Abstract:
:The gastrointestinal (GI) tract of metazoans is lined by a series of regionally distinct epithelia. To maintain structure and function of the GI tract, regionally diversified differentiation of somatic stem cell (SC) lineages is critical. The adult Drosophila midgut provides an accessible model to study SC regulation and specification in a regionally defined manner. SCs of the posterior midgut (PM) have been studied extensively, but the control of SCs in the middle midgut (MM) is less well understood. The MM contains a stomach-like copper cell region (CCR) that is regenerated by gastric stem cells (GSSCs) and contains acid-secreting copper cells (CCs). Bmp-like Decapentaplegic (Dpp) signaling determines the identity of GSSCs, and is required for CC regeneration, yet the precise control of Dpp signaling activity in this lineage remains to be fully established. Here, we show that Dad, a negative feedback regulator of Dpp signaling, is dynamically regulated in the GSSC lineage to allow CC differentiation. Dad is highly expressed in GSSCs and their first daughter cells, the gastroblasts (GBs), but has to be repressed in differentiating CCs to allow Dpp-mediated differentiation into CCs. We find that the Hox gene ultrabithorax (Ubx) is required for this regulation. Loss of Ubx prevents Dad repression in the CCR, resulting in defective CC regeneration. Our study highlights the need for dynamic control of Dpp signaling activity in the differentiation of the GSSC lineage and identifies Ubx as a critical regulator of this process.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Li H,Qi Y,Jasper Hdoi
10.1016/j.ydbio.2016.08.027subject
Has Abstractpub_date
2016-11-15 00:00:00pages
373-381issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(16)30337-2journal_volume
419pub_type
杂志文章abstract::The olfactory epithelium (OE) of the mouse provides a unique system for understanding how cell birth and cell death interact to regulate neuron number during development and regeneration. We have examined cell death in the OE in normal adult mice; in adult mice subjected to unilateral olfactory bulbectomy (surgical re...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.0025
更新日期:1995-11-01 00:00:00
abstract::Mutations that inactivate either merlin (mer) or expanded (ex) result in increased cell growth and proliferation in Drosophila. Both Mer and Ex are members of the Band 4.1 protein superfamily, and, based on analyses of mer ex double mutants, they are proposed to function together in at least a partially redundant mann...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.12.021
更新日期:2007-04-01 00:00:00
abstract::Epithelial-mesenchymal interactions are of major importance during development to direct correct differentiation and morphogenesis of embryonic tissues. One subset of lateral mesoderm-derived mesenchymal cells will form the smooth muscle (SM) layer of the primary epithelial lining of hollow internal organs. It has bee...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1207
更新日期:1994-08-01 00:00:00
abstract::In the Drosophila ventral nerve cord, the three pairs of Capability neuropeptide-expressing Va neurons are exclusively found in the second, third and fourth abdominal segments (A2-A4). To address the underlying mechanisms behind such segment-specific cell specification, we followed the developmental specification of t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.02.015
更新日期:2011-05-01 00:00:00
abstract::The development of the action potential and responses to neurotransmitters have been described for a population of embryonic spinal neurons developing in vivo. A comparable pattern is seen for spinal neurons developing in dissociated cell culture. The impulse appears very early in this developmental sequence, and the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90065-4
更新日期:1984-11-01 00:00:00
abstract::Evolutionary comparisons between Caenorhabditis elegans and the satellite organism Pristionchus pacificus revealed major differences in the regulation of nematode vulva development. For example, Wnt signaling is part of a negative signaling system that prevents vulva formation in P. pacificus, whereas it plays a posit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.01.034
更新日期:2006-05-01 00:00:00
abstract::Notch signaling has a fundamental role in stem cell maintenance and in cell fate choice in the intestine of different species. Canonically, Notch signaling represses the expression of transcription factors of the achaete-scute like (ASCL) or atonal related protein (ARP) families. Identifying the ARP/ASCL genes express...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.01.011
更新日期:2013-04-15 00:00:00
abstract::Segmentation is a key step in embryonic development. Acting in all germ layers, it is responsible for the generation of antero-posterior asymmetries. Hox genes, with their diverse expression in individual segments, are fundamental players in the determination of different segmental fates. In vertebrates, Hox gene prod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.016
更新日期:2010-07-01 00:00:00
abstract::The ciliated band of the Strongylocentrotus purpuratus embryo consists of a columnar epithelium, 3-5 cells wide, which gives rise to a small number of neuroblasts. It arises late in development, as a border separating the squamous epithelial cells of the oral and aboral ectoderm. To determine the lineage origins of th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1313
更新日期:1993-12-01 00:00:00
abstract::The human kidneys filter 180 l of blood every day via about 2.5 million glomeruli. The three layers of the glomerular filtration apparatus consist of fenestrated endothelium, specialized extracellular matrix known as the glomerular basement membrane (GBM) and the podocyte foot processes with their modified adherens ju...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.047
更新日期:2008-01-15 00:00:00
abstract::Mutations in the Bone Morphogenetic Protein (BMP) pathway are associated with a range of defects in skeletal formation. Genetic analysis of BMP signaling requirements is complicated by the presence of three partially redundant BMPs that are required for multiple stages of limb development. We generated an inducible al...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.07.003
更新日期:2014-09-15 00:00:00
abstract::Establishment and maintenance of functional stem cells is critical for organ development and tissue homeostasis. Little is known about the mechanisms underlying stem establishment during organogenesis. Drosophila testes are among the most thoroughly characterized systems for studying stem cell behavior, with germline ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.09.009
更新日期:2012-12-01 00:00:00
abstract::FoxC, FoxF, FoxL1 and FoxQ1 genes have been shown to be clustered in some animal genomes, with mesendodermal expression hypothesised as a selective force maintaining cluster integrity. Hypotheses are, however, constrained by a lack of data from the Lophotrochozoa. Here we characterise members of the FoxC, FoxF, FoxL1 ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.01.015
更新日期:2010-04-15 00:00:00
abstract::Monoclonal antibodies have been generated to determine the fate of sperm-specific surface components of the sea urchin, Arbacia punctulata, subsequent to gamete fusion. Monoclonal antibody-7 (MAB-7) reacted with two polypeptide bands having apparent molecular masses of 33 and 35 kDa derived from the sperm plasma membr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80036-3
更新日期:1989-01-01 00:00:00
abstract::The chicken retina is capable of limited regeneration. In response to injury, some Müller glia proliferate and de-differentiate into progenitor cells. However, most of these progenitors fail to differentiate into neurons. The Notch pathway is upregulated during retinal regeneration in both fish and amphibians. Since t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.09.046
更新日期:2007-12-01 00:00:00
abstract::Cell proliferation, growth and survival are three different basic processes which converge at determining a fundamental property -the size of an organism. Scalloped (Sd) is the first characterised transcriptional partner to Yorkie (Yki), the downstream effector of the Hippo pathway which is a highly potential and evol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.10.016
更新日期:2017-12-15 00:00:00
abstract::Cell-cell fusion is a fundamental cellular process that is essential for development as well as fertilization. Myoblast fusion to form multinucleated skeletal muscle myotubes is a well studied, yet incompletely understood example of cell-cell fusion that is essential for formation of contractile skeletal muscle tissue...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.035
更新日期:2009-01-15 00:00:00
abstract::The diaphragm is a mammalian skeletal muscle essential for respiration and for separating the thoracic and abdominal cavities. Development of the diaphragm requires the coordinated development of muscle, muscle connective tissue, tendon, nerves, and vasculature that derive from different embryonic sources. However, de...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.04.010
更新日期:2018-08-15 00:00:00
abstract::Most, if not all, endocrine cells seem capable of synthesizing and storing more than one hormone. Such cellular colocalization of hormones can be due either to the presence of two or more specific granules within the cells or to colocalization of the hormones within a single granule. The present study was performed to...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90122-w
更新日期:1992-10-01 00:00:00
abstract::Gastrulation in Xenopus laevis transforms a ball of undifferentiated cells into a well-organized elongated embryo with a distinct body axis, as a result of extensive cell rearrangements and movements. Since cell adhesion is thought to play an important role during tissue morphogenesis, we investigated the potential ro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1288
更新日期:1995-10-01 00:00:00
abstract::c-fms and c-kit are structurally related tyrosine kinase receptors for colony-stimulating factor-1 (CSF-1) and for steel factor (SF), respectively. The level of c-fms mRNA, like c-kit mRNA, increases during the maturation of oocytes and is an abundant maternal message found in the unfertilized oocyte. Following fertil...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90207-w
更新日期:1992-05-01 00:00:00
abstract::Only a subset of cleavage stage blastomeres in the Xenopus embryo is competent to contribute cells to the retina; ventral vegetal blastomeres do not form retina even when provided with neuralizing factors or transplanted to the most retinogenic position of the embryo. These results suggest that endogenous maternal fac...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.040
更新日期:2007-05-01 00:00:00
abstract::The Jonah gene family consists of approximately 20 genes, distributed in small clusters at eight or more widely dispersed chromosomal sites. Gene clusters differ in the number of genes per cluster, ranging up to four, and in the arrangement of genes within clusters, which include direct repeats, inverted repeats, and ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90038-7
更新日期:1985-04-01 00:00:00
abstract::Type II collagen is a major component of cartilage extracellular matrix. Differentiation of mesenchyme into cartilage involves the cessation of type I collagen synthesis and the onset of type II collagen synthesis. Solution hybridization of mRNA isolated from chick limb buds with a cDNA probe to type II collagen mRNA ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90057-7
更新日期:1988-01-01 00:00:00
abstract::The finding that retinoic acid (RA) has major effects on pattern formation in developing chick limbs has led to the conclusion that RA plays a central role in the normal development of the limb. In addition, it has been demonstrated that treatment with exogenous RA leads to changes in expression of homeobox-containing...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1301
更新日期:1994-11-01 00:00:00
abstract::The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8915
更新日期:1998-07-01 00:00:00
abstract::Border Cells in the Drosophila ovaries are a useful genetic model for understanding the molecular events underlying epithelial cell motility. During stage 9 of egg chamber development they detach from neighboring stretched cells and migrate between the nurse cells to reach the oocyte. RNAi screening allowed us to iden...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.05.017
更新日期:2012-08-01 00:00:00
abstract::The sub-division of the posterior-most territory of the neural plate results in the formation of two distinct neural structures, the hindbrain and the spinal cord. Although many of the molecular signals regulating the development of these individual structures have been elucidated, the mechanisms involved in delineati...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.12.025
更新日期:2016-02-15 00:00:00
abstract::Although the development of several of the voltage-dependent currents in embryonic amphibian myocytes has been described, the overall muscle electrical development, particularly the relative times of expression of different voltage-dependent currents, has not been addressed in a single study under one set of condition...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90043-g
更新日期:1992-11-01 00:00:00
abstract::In vertebrates, the Müllerian duct elongates along the Wolffian duct, a mesonephric structure that is required for Müllerian duct formation. Recently, several genes required for initial Müllerian duct formation have been identified. However, the precise mechanism of Müllerian duct elongation remains to be elucidated. ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.027
更新日期:2009-01-15 00:00:00