Abstract:
:Evolutionary comparisons between Caenorhabditis elegans and the satellite organism Pristionchus pacificus revealed major differences in the regulation of nematode vulva development. For example, Wnt signaling is part of a negative signaling system that prevents vulva formation in P. pacificus, whereas it plays a positive role in C. elegans. We wondered if the genetic control of the second major part of the nematode egg-laying system, the sex muscles, has diverged similarly between P. pacificus and C. elegans. The sex muscles derive from the mesoblast M, which has an identical lineage in both species. Here, we describe a large-scale mutagenesis screen for mutations that disrupt the M lineage and the sex myoblast (SM) sublineage. We isolated and characterized mutations that result in a failure of proper SM fate specification and SM migration and showed that the corresponding genes encode Ppa-sem-4 and Ppa-egl-17, respectively. Ppa-sem-4 mutants have additional defects in the specification of the vulva precursor cells P(5, 7).p and experimental studies in the Ppa-egl-17 mutant background indicate a complex set of gonad-dependent and gonad-independent mechanisms required for SM migration. Mutations in Cel-sem-4 and Cel-egl-17 cause similar defects. Thus, the molecular mechanisms of SM cell specification and migration are conserved between P. pacificus and C. elegans.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Photos A,Gutierrez A,Sommer RJdoi
10.1016/j.ydbio.2006.01.034subject
Has Abstractpub_date
2006-05-01 00:00:00pages
142-53issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(06)00071-6journal_volume
293pub_type
杂志文章abstract::The germinal vesicle (GV) of starfish oocytes contains a factor which is required to drive the cytoplasmic cycle of the meiotic division. Biochemical investigation of this factor has been difficult due to the small quantities of obtainable GV materials. To overcome this, we have developed a mass-isolation procedure fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90187-l
更新日期:1992-06-01 00:00:00
abstract::The cDNA encoding the TATA binding protein was isolated from 8- to 16-cell and morula-stage embryonic libraries of two distantly related species of sea urchin, Strongylocentrotus purpuratus and Lytechinus variegatus, respectively. The two proteins are 96% identical over both the N- and C-terminal domains, suggesting a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9052
更新日期:1998-12-01 00:00:00
abstract::Selected stages of the developing chicken cornea have been examined for type VI collagen, employing monoclonal antibodies specific for this molecule. By immunofluorescence, the molecule is not detectable in 5 1/2 day corneas, a time at which the epithelial-derived, acellular primary stroma is the only corneal matrix p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90013-8
更新日期:1986-12-01 00:00:00
abstract::Huntington disease (HD) is an adult-onset neurodegenerative disease characterized by motor, cognitive, and psychiatric symptoms that is caused by a CAG expansion in the HTT gene. Palmitoylation is the addition of saturated fatty acids to proteins by DHHC palmitoylacyl transferases. HTT is palmitoylated by huntingtin i...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.11.018
更新日期:2015-01-15 00:00:00
abstract::Drosophila thoracic muscles are comprised of both direct flight muscles (DFMs) and indirect flight muscles (IFMs). The IFMs can be further subdivided into dorsolongitudinal muscles (DLMs) and dorsoventral muscles (DVMs). The correct patterning of each category of muscles requires the coordination of specific executive...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(03)00255-0
更新日期:2003-08-15 00:00:00
abstract::The fate of bacteriophage lambda-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of lambda-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90155-7
更新日期:1986-08-01 00:00:00
abstract::Monoclonal antibodies (mAbs), electrophoresis, immunoblotting, and immunohistochemistry were used to determine the molecular properties of cardiac myosin heavy chain (MHC) isoforms and the regions of the developing chicken heart in which they were expressed. Adult atria expressed three electrophoretically distinct MHC...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90324-7
更新日期:1988-06-01 00:00:00
abstract::The human kidneys filter 180 l of blood every day via about 2.5 million glomeruli. The three layers of the glomerular filtration apparatus consist of fenestrated endothelium, specialized extracellular matrix known as the glomerular basement membrane (GBM) and the podocyte foot processes with their modified adherens ju...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.047
更新日期:2008-01-15 00:00:00
abstract::Thoracic sensory neurons in bullfrog tadpoles can be induced to form connections typical of brachial sensory neurons by transplanting thoracic ganglia to the branchial level at stages when some thoracic sensory neurons already have formed connections. In order to find out how many postmitotic sensory neurons survive t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90369-t
更新日期:1990-12-01 00:00:00
abstract::The zebrafish T-box transcription factors spadetail (spt) and the brachyury ortholog no tail (ntl) are together essential for posterior mesoderm formation. In addition to being functionally redundant, spt and ntl also genetically interact with zygotic mutant alleles of one-eyed pinhead (Zoep), leading to synergistic m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.09.008
更新日期:2003-12-15 00:00:00
abstract::One of the earliest steps in embryonic development is the establishment of the future body axes. Morphological and molecular data place the Ambulacraria (echinoderms and hemichordates) within the Deuterostomia and as the sister taxon to chordates. Extensive work over the last decades in echinoid (sea urchins) echinode...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.030
更新日期:2011-06-01 00:00:00
abstract::The development of the Drosophila leg is a good model to study processes of pattern formation, cell death and segmentation. Such processes require the coordinate activity of different genes and signaling pathways that progressively subdivide the leg territory into smaller domains. One of the main pathways needed for l...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2013.10.011
更新日期:2014-01-15 00:00:00
abstract::Two populations of blastomeres become positionally distinct during fourth cleavage in the mouse embryo; the inner cells become enclosed within the embryo and the outer cells form the enclosing layer. The segregation of these two cell populations is important for later development, because it represents the initial ste...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(90)90003-2
更新日期:1990-01-01 00:00:00
abstract::Inverse gradients of transcriptional repressors antagonize the transcriptional effector response to morphogens. However, the role of such inverse regulation might not manifest solely from lack of repressors. Sonic hedgehog (Shh) patterns the forebrain by being expressed ventrally; however, absence of antagonizing Gli3...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.03.011
更新日期:2019-06-01 00:00:00
abstract::During endochondral ossification, the differentiation of proliferating into hypertrophic chondrocytes is a key step determining the pace of bone formation and the future length of the skeletal elements. A variety of transcription factors are expressed at the onset of hypertrophy coordinating the expression of differen...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.09.012
更新日期:2020-01-01 00:00:00
abstract::The serine/threonine kinase LKB1 is a master kinase that regulates a number of critical events such as cell transformation, polarization, development, stress response, and energy metabolism in metazoa. After multiple unsuccessful attempts of generating Dictyostelium lkb1-null cells, an RNAi-based knockdown approach pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.10.006
更新日期:2011-12-15 00:00:00
abstract::The relative sizes of the various structures in Hydra attenuata were compared over a broad range of animal sizes to determine in detail the ability to regulate proportions during regeneration. The three components of the head, namely hypostome, tentacles, and tentacle zone from which the tentacles emerge, the body col...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90318-x
更新日期:1984-06-01 00:00:00
abstract::To analyze the molecular mechanism of the limb pattern formation, we have tried to make monoclonal antibodies against antigens from chick limb buds. We obtained one antibody named AV-1 which recognized a specific region of chick limb buds. AV-1 reacted with the distal portion of the anteroventral mesoderm of only deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90337-4
更新日期:1986-10-01 00:00:00
abstract::NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.013
更新日期:2008-05-15 00:00:00
abstract::The MEI-1/MEI-2 microtubule-severing complex, katanin, is required for oocyte meiotic spindle formation and function in C. elegans, but the microtubule-severing activity must be quickly downregulated so that it does not interfere with formation of the first mitotic spindle. Post-meiotic MEI-1 inactivation is accomplis...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.04.004
更新日期:2009-06-15 00:00:00
abstract::Inhibitor of differentiation and DNA-binding (Id) proteins, Id1 to Id4, function in the regulation of cellular proliferation and differentiation. Id proteins have been shown to interact with bHLH proteins and other proteins involved in regulating cellular proliferation and differentiation, suggesting a widespread regu...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.12.006
更新日期:2020-04-15 00:00:00
abstract::Eggs and embryos of the sea urchin Lytechinus pictus were labeled with [35S]methionine. Aqueous extracts of protein were prepared and analyzed by a high resolution two-dimensional polyacrylamide gel electrophoresis system described recently by O'Farrell utilizing isoelectric focusing and sodium dodecyl sulfate electro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(76)90248-7
更新日期:1976-09-01 00:00:00
abstract::Expression of constitutively active Src protein tyrosine kinase in Xenopus oocytes has been shown to accelerate oocyte maturation suggesting that Src may be involved in meiotic progression. However, meiotic regulation of endogenous Src kinase in oocytes has not been investigated in detail. To address this problem, we ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.10.018
更新日期:2005-02-15 00:00:00
abstract::The t haplotypes are variant forms of the proximal portion of chromosome 17 in the mouse. The t haplotypes alter spermatogenesis and many also contain lethal factors. Although the lethal factors vary between t haplotypes, all t haplotypes have the same effect on sperm, that of altering sperm function in fertilization....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1002
更新日期:1993-01-01 00:00:00
abstract::Extensive elongation of lens fiber cells is a central feature of lens morphogenesis. Our study investigates the role of N-cadherin junctions in this process in vivo. We investigate both the molecular players involved in N-cadherin junctional maturation and the subsequent function of these junctions as epicenters for t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.10.009
更新日期:2011-01-15 00:00:00
abstract::Serendipity delta (sry delta) is a member of a set of Drosophila zinc finger protein genes showing maximal transcription during oogenesis. By using transformant lines, we monitored the zygotic expression of the sry delta gene and characterized some biochemical properties of a sry delta/beta-galactosidase fusion protei...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90272-8
更新日期:1989-12-01 00:00:00
abstract::Conjugation in Tetrahymena represents an ordered developmental pathway which represents the sexual phase of the ciliate life cycle. This pathway is initiated when starved cells of opposite mating types are mixed and are allowed to make a series of cell-cell contacts (a period termed costimulation) which lead to the fo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90075-e
更新日期:1991-02-01 00:00:00
abstract::The rabbit sperm membrane autoantigen RSA-1 is a sialoglycoprotein of 13,000 daltons which first appears on the surface of pachytene spermatocytes. Using specific antiserum to RSA-1 the antigen has been localized by immunofluorescence and immunoperoxidase staining. On testicular cells labeled at 37 degrees C, RSA-1 is...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(81)90400-0
更新日期:1981-06-01 00:00:00
abstract::The lizards are evolutionarily the closest vertebrates to humans that demonstrate the ability to regenerate entire appendages containing cartilage, muscle, skin, and nervous tissue. We previously isolated PAX7-positive cells from muscle of the green anole lizard, Anolis carolinensis, that can differentiate into multin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.08.037
更新日期:2018-01-15 00:00:00
abstract::Pax3/7 paired homeodomain transcription factors are important markers of muscle stem cells. Pax3 is required upstream of myod for lateral dermomyotomal cells in the amniote somite to form particular muscle cells. Later Pax3/7-dependent cells generate satellite cells and most body muscle. Here we analyse early myogenes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.009
更新日期:2007-02-15 00:00:00