Abstract:
:The zebrafish T-box transcription factors spadetail (spt) and the brachyury ortholog no tail (ntl) are together essential for posterior mesoderm formation. In addition to being functionally redundant, spt and ntl also genetically interact with zygotic mutant alleles of one-eyed pinhead (Zoep), leading to synergistic mesodermal defects. Here we have used genetic and pharmacological assays to address the mechanism of these interactions. We show that Zoep and ntl are together required upstream of spt expression, accounting for the severity of the mesodermal defects in Zoep;ntl embryos. Since Xenopus brachyury is proposed to regulate fgf expression, and FGF signaling is required for spt expression, we analyzed the involvement of the FGF signaling pathway in these genetic interactions. Using a specific inhibitor of FGFR activity to indirectly assay the strength of FGF signaling in individual embryos, we found that spt and ntl mutant embryos were both hypersensitive to the FGFR inhibitor. This hypersensitivity is consistent with the possibility that Spt and Ntl function upstream of FGF signaling. Furthermore, we show that minor pharmacological or genetic perturbations in FGF signaling are sufficient to dramatically enhance the Zoep mutant phenotype, providing a plausible explanation for why Zoep genetically interacts with spt and ntl. Finally, we show that Zoep and ace/fgf8 function are essential for the formation of all posterior tissues, including spinal cord. Taken together, our data provide strong in vivo support for the regulation of FGF signaling by T-box transcription factors, and the cooperative activity of Oep and FGF signaling during the formation of posterior structures.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Griffin KJ,Kimelman Ddoi
10.1016/j.ydbio.2003.09.008subject
Has Abstractpub_date
2003-12-15 00:00:00pages
456-66issue
2eissn
0012-1606issn
1095-564Xpii
S001216060300530Xjournal_volume
264pub_type
杂志文章abstract::Primitive erythropoiesis is regulated in a non cell-autonomous fashion across evolution from frogs to mammals. In Xenopus laevis, signals from the overlying ectoderm are required to induce the mesoderm to adopt an erythroid fate. Previous studies in our lab identified the transcription factor GATA2 as a key regulator ...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90445-3
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90417-2
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9727
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.09.014
更新日期:2014-11-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80058-2
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1112
更新日期:1993-05-01 00:00:00
abstract::This study describes the temporal pattern of posterior positional identity in mouse limb bud cells. To do this wedges of tissue from the posterior edge of mouse limb buds at various stages (limb stages: Wanek et al., 1989b. J. Exp. Zool. 249, 41-49) were grafted to the anterior edge of a host chick embryo wing bud. Gr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90306-n
更新日期:1991-10-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.01.045
更新日期:2008-05-01 00:00:00
abstract::Oral-facial-digital type I (OFDI) syndrome is an X-linked male lethal developmental disorder. It is ascribed to ciliary dysfunction and characterized by malformation of the face, oral cavity, and digits. Conditional inactivation using different Cre lines allowed us to study the role of the Ofd1 transcript in limb deve...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.09.020
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abstract::To investigate the mechanism by which the hormone 17 alpha,20 beta-dihydroxy-4-pregnen-3-one(17 alpha,20 beta-DP) acts on a receptor on the external surface of rainbow trout oocytes to induce maturation, the interaction between 17 alpha,20 beta-DP receptors and G-proteins was examined. Pertussis toxin (PT) catalyzed t...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1341
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90006-x
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pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9120
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90143-5
更新日期:1992-08-01 00:00:00
abstract::We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (blood vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9246
更新日期:1999-05-01 00:00:00