Abstract:
:We have investigated the regulation of DNA synthesis in the heterokaryons of HL60 human myelomonocytic leukemia cells and NIH3T3 mouse fibroblasts to examine if the differentiated leukemia cells contained a replication inhibiting activity. Cell fusions were performed either by exposing a suspension of mixed cells to an electric pulse or by the polyethylene glycol method. To identify the origin of the nuclei in a heterokaryon, one set of partner cells was prelabeled with [3H]thymidine before fusion. DNA synthetic activity after fusion was then revealed immunohistochemically by bromodeoxyuridine incorporation. DNA synthesis in the nuclei of 3T3 was inhibited in the heterokaryons of 3T3 and in either one of the two differentiated forms of HL60, i.e., the macrophage-like or the granulocyte-like. The result supports that a negative regulator of DNA synthesis exists in the differentiated HL60. Surprisingly, we have also found that DNA synthesis was inhibited in the nuclei of both 3T3 and nondifferentiated, proliferating HL60 when these two cells were fused. When unfused, proliferating cells were eliminated with cytosine arabinoside; these nonreplicating heterokaryons survived for at least 5 days, and 15% of them showed alpha-naphthylacetate esterase activity, a trait of the macrophage differentiation. The blockage of DNA synthesis in both partner nuclei was also observed in the heterokaryons of NIH3T3 cells and nondifferentiated human promonocytic leukemia cells U937, and in nondifferentiated HL60 and human diploid fibroblasts WI38. However, this effect was not found in the heterokaryons of NIH3T3 cells and human B lymphoma WI-729-HF2 cells. This is the first demonstration of the inhibition of DNA synthesis upon fusion of two proliferating cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Prudovsky IA,Tsong TYdoi
10.1016/0012-1606(91)90417-2subject
Has Abstractpub_date
1991-04-01 00:00:00pages
232-9issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(91)90417-2journal_volume
144pub_type
杂志文章abstract::The Toll signaling pathway functions in several Drosophila processes, including dorsal-ventral pattern formation and the immune response. Here, we demonstrate that this pathway is required in the epidermis for proper muscle development. Previously, we showed that the zygotic Toll protein is necessary for normal muscle...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8915
更新日期:1998-07-01 00:00:00
abstract::Gastrulation is the first major morphogenetic movement in development and requires dynamic regulation of cell adhesion and the cytoskeleton. Caenorhabditis elegans gastrulation begins with the migration of the two endodermal precursors, Ea and Ep, from the surface of the embryo into the interior. Ea/Ep migration provi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.05.507
更新日期:2010-08-15 00:00:00
abstract::The role of angiotensin II (Ang II) in the early embryonic development of the heart has not been examined. We have used RT-PCR to identify mRNA for angiotensinogen, angiotensin-converting enzyme, and the Ang II AT1 and AT2 receptors in embryonic day 10.25 Sprague-Dawley rats, and have used confocal microscopy to local...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8764
更新日期:1997-12-15 00:00:00
abstract::Trichosia pubescens larvae were ligated at different prepupal developmental stages in order to investigate whether ecdysterone acts just as a trigger or whether its presence in the hemolymph is constantly necessary for the induction of the late puffs, in particular the DNA puffs. The larvae were ligated in the middle ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90170-2
更新日期:1984-03-01 00:00:00
abstract::The zebrafish T-box transcription factors spadetail (spt) and the brachyury ortholog no tail (ntl) are together essential for posterior mesoderm formation. In addition to being functionally redundant, spt and ntl also genetically interact with zygotic mutant alleles of one-eyed pinhead (Zoep), leading to synergistic m...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.09.008
更新日期:2003-12-15 00:00:00
abstract::The relationship among neuronal type, position, and time course of development of identified neurons was examined in the zebrafish (Brachydanio rerio). The cells studied, the reticulospinal neurons Mauthner, MiM1, and MiV1, are located within the same small region in the hindbrain, differ stereotypically in their posi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90422-1
更新日期:1985-12-01 00:00:00
abstract::Three unrelated chromosome 2q14.1-14.2 region translocations caused the split hand/foot limb malformation development in humans by an unknown mechanism. Their etiology was described by the autosomal dominant inheritance with incomplete penetrance genetic model although authors stated, "the understanding of the genotyp...
journal_title:Developmental biology
pub_type: 杂志文章,评审
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更新日期:2015-12-01 00:00:00
abstract::It has long been hypothesized that in fishes the contents of cortical granules are involved in the hardening of egg envelope following fertilization. We previously purified the egg envelope hardening initiation factor from the exudates released from activated medaka (Oryzias latipes) eggs and tentatively termed this p...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.09.016
更新日期:2012-12-15 00:00:00
abstract::Mesenchyme to epithelium transitions are crucial to embryonic development. The early mouse embryo offers an excellent model to study epithelium formation as during the first three days of development two epithelia are formed, the trophectoderm (TE) and the primitive endoderm (PrE). We have previously shown that PrE ce...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.10.048
更新日期:2008-01-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1325
更新日期:1993-12-01 00:00:00
abstract::External genital development begins with formation of paired genital swellings, which develop into the genital tubercle. Proximodistal outgrowth and axial patterning of the genital tubercle are coordinated to give rise to the penis or clitoris. The genital tubercle consists of lateral plate mesoderm, surface ectoderm,...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0668
更新日期:2002-07-01 00:00:00
abstract::About 12-17% of the embryos obtained by mating mice carrying the In(X)1H or Paf mutations are of the 39,X (X0) genotype. Depending on the mutant mice used for mating, the monosomic X chromosome can be inherited from the paternal (XP) or the maternal (XM) parent. The XP0 embryos display developmental retardation at gas...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.8972
更新日期:1998-09-01 00:00:00
abstract::Retinoic acid (RA) plays a critical role in neural patterning and organogenesis in the vertebrate embryo. Here we characterize a mutant of the zebrafish named giraffe (gir) in which the gene for the RA-degrading enzyme Cyp26a1 is mutated. The gir mutant displayed patterning defects in multiple organs including the com...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.11.023
更新日期:2005-02-15 00:00:00
abstract::Trypsin-like activity is secreted from eggs of many species at fertilization, and this activity is believed to be critical for the block to polyspermy. Here we show that a cortical granule serine protease of sea urchins is the major and perhaps only protease family member important for fertilization. Zymography assays...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9299
更新日期:1999-07-01 00:00:00
abstract::The NCK adaptor proteins are composed entirely of SH3 and SH2 domains and serve as protein interaction bridges for several receptors during signal transduction events. Here we report the molecular and genetic analysis of the Caenorhabditis elegans nck-1 gene. C. elegans nck-1 encodes two isoforms: NCK-1A and a shorter...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.03.019
更新日期:2011-06-01 00:00:00
abstract::The shoot meristem generates all of the aerial structures of an adult plant. It is organized in three layers which produce the epidermis (L1 layer) and the subepidermal layers (L2 and L3). The origin of adult structures has previously been fate mapped to the primary meristem for L2 and L3 tissues. In this work we cons...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0112
更新日期:1996-05-01 00:00:00
abstract::A pair of the Drosophila eye-antennal disc gives rise to four distinct organs (eyes, antennae, maxillary palps, and ocelli) and surrounding head cuticle. Developmental processes of this imaginal disc provide an excellent model system to study the mechanism of regional specification and subsequent organogenesis. The do...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.06.015
更新日期:2011-08-15 00:00:00
abstract::Conserved from Cnidarians to vertebrates, the dorsoventral (DV) axis is patterned by the Chordin-BMP pathway. However, the functions of the pathway's components are very different in different phyla. By modeling it is shown that many observations can be integrated by the assumption that BMP, acting as an inhibitory co...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.05.025
更新日期:2015-09-01 00:00:00
abstract::The Drosophila Suppressor of Hairy wing [Su(Hw)] insulator protein has an essential role in the development of the female germline. Here we investigate the function of Su(Hw) in the ovary. We show that Su(Hw) is universally expressed in somatic cells, while germ cell expression is dynamic. Robust levels accumulate in ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.05.666
更新日期:2011-08-15 00:00:00
abstract::Symplastic spermatids (sys) male mice are sterile due to a recessive mutation that causes defective adhesion between spermatids and Sertoli cells within the seminiferous epithelium. We show that the mutation in sys mice involves a deletion of 1.24 Mb of chromosome 14. Comparative genomic analysis suggests that this re...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.054
更新日期:2006-10-15 00:00:00
abstract::Type II collagen is a major component of cartilage extracellular matrix. Differentiation of mesenchyme into cartilage involves the cessation of type I collagen synthesis and the onset of type II collagen synthesis. Solution hybridization of mRNA isolated from chick limb buds with a cDNA probe to type II collagen mRNA ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90057-7
更新日期:1988-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90239-x
更新日期:1989-04-01 00:00:00
abstract::We cloned Xenopus Strabismus (Xstbm), a homologue of the Drosophila planar cell or tissue polarity gene. Xstbm encodes four transmembrane domains in its N-terminal half and a PDZ-binding motif in its C-terminal region, a structure similar to Drosophila and mouse homologues. Xstbm is expressed strongly in the deep cell...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0673
更新日期:2002-07-01 00:00:00
abstract::The ectoderm of early Xenopus gastrula is competent to become induced to neural tissue, but dorsal ectoderm is more neural competent than ventral ectoderm. It is a tenable, but as yet untested possibility that the higher neural competence of dorsal gastrula ectoderm is dependent on the presence of the dorsal mesoderm....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90168-g
更新日期:1992-07-01 00:00:00
abstract::The rabbit ovum seldom becomes polyspermic despite the presence of supernumerary sperm with easy access to the ovum plasma membrane. The purpose of this study was to characterize the role of ovum investments in blocking polyspermy. Ova were inseminated with capacitated sperm in vitro and were fertilized. Early stages ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90241-7
更新日期:1987-04-01 00:00:00
abstract::The purpose of this study was to examine part of the trophoblast cell multilineage pathway and its modulation by retinoic acid. A method for studying trophoblast cell differentiation along the spongiotrophoblast cell pathway in vitro was established and characterized. Cells were isolated from junctional zones of Day 1...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1125
更新日期:1994-05-01 00:00:00
abstract::In Xenopus, the hatching and cement glands form at important boundaries in the head. We have examined induction of these glands in order to see the basis for this ectodermal patterning. Ectoderm can be induced to form hatching and cement glands by dorsal mesoderm and by the neural plate. Because the neural plate is su...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1993.1183
更新日期:1993-07-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90236-3
更新日期:1987-04-01 00:00:00
abstract::The Drosophila abnormal wing discs (awd) gene encodes the subunit of a protein that has nucleoside diphosphate kinase (NDP kinase) activity. Null mutations of the awd gene cause lethality after puparium formation. Larvae homozygous for such mutations have small imaginal discs, lymph glands, and brain lobes. Neither th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1996.0184
更新日期:1996-08-01 00:00:00
abstract::The embryonic vertebrate heart consists of two epithelia: the myocardium and endothelium, separated by the myocardial basement membrane (MBM). The myocardium has been shown to induce endothelial transformation into prevalvular mesenchyme in a temporally and site restricted manner. Previously, we hypothesized that the ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(89)90135-8
更新日期:1989-11-01 00:00:00