Abstract:
:The zebrafish hedgehog (hh) family members tiggy-winkle hedgehog (twhh) and sonic hedgehog (shh) are involved in patterning the ventral CNS and proximal eye. Using a dominant negative protein kinase A regulatory subunit mutant, we show that these hh activities are mediated by protein kinase A. The effects of dominant negative protein kinase A on pax2 expression appear to be cell nonautonomous, suggesting that cells can respond to regulation of hh signaling by modulating an additional cell-cell signaling pathway. We also investigate the potential involvement of cyclops in the hh signaling pathway and conclude that although cyclops mutant cells can respond to hh signaling, neither hh nor dominant negative protein kinase A rescues the phenotypes associated with cyclops.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Ungar AR,Moon RTdoi
10.1006/dbio.1996.0209subject
Has Abstractpub_date
1996-08-25 00:00:00pages
186-91issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(96)90209-2journal_volume
178pub_type
杂志文章abstract::Transgenic mice expressing stabilized beta-catenin in neural progenitors develop enlarged brains resulting from increased progenitor expansion. To more precisely define beta-catenin regulation of progenitor fate, we employed a conditional transgenic approach to delete the beta-catenin regulatory domain from neural pro...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.07.013
更新日期:2007-09-15 00:00:00
abstract::We have cloned a Caenorhabditis elegans homologue of the Drosophila gap gene hunchback (hb) and have designated it hbl-1 (hunchback-like). hbl-1 encodes a predicted 982-amino-acid protein, containing two putative zinc-finger domains similar to those of Drosophila Hunchback. The gene is transcribed embryonically, but u...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1998.9096
更新日期:1999-01-15 00:00:00
abstract::Quantification of GFP reporter gene expression at single cell level in living sea urchin embryos can now be accomplished by a new method of confocal laser scanning microscopy (CLSM). Eggs injected with a tissue-specific GFP reporter DNA construct were grown to gastrula stage and their fluorescence recorded as a series...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.06.016
更新日期:2006-11-15 00:00:00
abstract::We describe mesendoderm morphogenesis during gastrulation in the frog Xenopus laevis and investigate the mechanics of these movements with tissue explants. When a dorsal marginal zone explant is plated onto fibronectin, the mesendoderm moves away from the dorsal axial tissues as an intact sheet. Mesendodermal cells wi...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0537
更新日期:2002-02-15 00:00:00
abstract::We investigated whether the inhibition of proteoglycan synthesis and salivary branching morphogenesis by beta-D-xyloside was related to the deposition and processing of newly synthesized glycosaminoglycans at the basal epithelial surface that correlates with normal branching activity. Forty eight-hour cultures of cont...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90358-6
更新日期:1985-05-01 00:00:00
abstract::We have used in situ hybridization and RNA blotting analysis to compare the timing of accumulation of poly(A) and alpha-subtype histone mRNA during oogenesis in the sea urchin Strongylocentrotus purpuratus. In situ hybridization with 3H-poly(U) shows that the content of poly(A) in the developing oocyte increases four-...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90161-1
更新日期:1984-02-01 00:00:00
abstract::Distal-less 3 (DLX3) gene mutations are etiologic for Tricho-Dento-Osseous syndrome. To investigate the in vivo impact of mutant DLX3 on bone development, we established transgenic (TG) mice expressing the c.571_574delGGGG DLX-3 gene mutation (MT-DLX3) driven by a mouse 2.3 Col1A1 promoter. Microcomputed tomographic a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.10.014
更新日期:2009-01-01 00:00:00
abstract::Developmental boundaries ensure that cells fated to participate in a particular structure are brought together or maintained at the appropriate locale within developing embryos. Parasegment grooves mark the position of boundaries that separate every segment of the Drosophila embryo into anterior and posterior compartm...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.026
更新日期:2008-09-15 00:00:00
abstract::Lumenogenesis of small seamless tubes occurs through intracellular membrane growth and directed vesicle fusion events. Within the Caenorhabditis elegans excretory cell, which forms seamless intracellular tubes (canals) that mediate osmoregulation, lumens grow in length and diameter when vesicles fuse with the expandin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.07.019
更新日期:2014-10-01 00:00:00
abstract::The sea urchin sperm receptor isolated from the eggs of Strongylocentrotus purpuratus is a high molecular weight proteoglycan-like molecule. Previous studies in our laboratory suggested that the sperm receptor has two functional components, glycosaminoglycan chains that are responsible for sperm binding and polypeptid...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90088-6
更新日期:1986-11-01 00:00:00
abstract::The lateral line system displays highly divergent patterns in adult teleost fish. The mechanisms underlying this variability are poorly understood. Here, we demonstrate that the lateral line mechanoreceptor, the neuromast, gives rise to a series of accessory neuromasts by a serial budding process during postembryonic ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.02.017
更新日期:2010-04-15 00:00:00
abstract::The mouse protamine mRNAs, Prm-1 and Prm-2, are translationally repressed for several days during male germ cell differentiation. The translational delay of mouse Prm-1 mRNA has previously been shown to be dependent upon cis-acting elements that reside in the last 62 nucleotides of the Prm-1 3' untranslated region (3'...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8705
更新日期:1997-11-01 00:00:00
abstract::We have investigated the sequential changes of acetylcholine receptor (AChR) distribution on identified Xenopus laevis muscle cells in culture before and after innervation. AChRs on muscle cells were stained with tetramethylrhodamine-conjugated alpha-bungarotoxin and the distribution of AChR clusters was examined on a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90006-x
更新日期:1984-05-01 00:00:00
abstract::The calmodulin levels in stage 6 Xenopus oocytes averaged 89 +/- 24 (SD) ng/oocyte and had largely accumulated by stage 3 of oogenesis. From stage 3 to early stage 6, calmodulin levels did not increase further. However, in large stage 6 oocytes (greater than 1.25 mm diam) calmodulin levels again rose to a level as hig...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90120-x
更新日期:1986-01-01 00:00:00
abstract::Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron pr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.08.009
更新日期:2016-10-01 00:00:00
abstract::Many insects undergo katatrepsis, essential reorganization by the extraembryonic membranes that repositions the embryo. Knockdown of the zen gene by RNA interference (RNAi) prevents katatrepsis in the milkweed bug Oncopeltus fasciatus. However, the precise morphogenetic defect has been uncertain, and katatrepsis itsel...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.06.036
更新日期:2009-09-15 00:00:00
abstract::Treatment of metaphase II-arrested hamster eggs with activators of protein kinase C has been reported to promote resumption of the cell cycle, second polar body emission, and pronucleus formation (G.I. Gallicano, S.M. Schwarz, R.W. McGaughey, and D.G. Capco, 1993, Dev. Biol. 156, 94-106). In contrast, we have not obse...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1233
更新日期:1995-08-01 00:00:00
abstract::Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we chara...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2012.12.009
更新日期:2013-02-15 00:00:00
abstract::Melanoblasts, the precursors of the pigment-producing cells of the skin and hair, are derived from the neural crest and migrate to the skin around 12 days of gestation in the mouse. In adult mice almost all the melanoblasts are confined to the hair follicles except for the epidermal layers of nonhairy skin. The recept...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9856
更新日期:2000-09-15 00:00:00
abstract::T-box family transcription factors play many roles in Metazoan development. Here we characterise Tbx6r, a unique Tbx6 paralogue isolated from the amphibian Xenopus. The evolution and developmental integration of this divergent T-box gene within the vertebrates reveals an unexpected level of plasticity within this cons...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.01.013
更新日期:2010-04-01 00:00:00
abstract::Retinal stem cells (RSCs) exist as rare pigmented ciliary epithelial cells in adult mammalian eyes. We hypothesized that RSCs are at the top of the retinal cell lineage. Thus, genes expressed early in embryonic development to establish the retinal field in forebrain neuroectoderm may play important roles in RSCs. Pax6...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.01.021
更新日期:2007-04-15 00:00:00
abstract::cAMP chemoattractant receptors on the surface of Dictyostelium discoideum cells are visualized by means of immunocytochemistry. Receptor antigen is virtually absent from growing cells and begins to accumulate after about 6 hr of starvation, concomitant with the increase in surface cAMP binding activity. In aggregating...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90268-0
更新日期:1988-07-01 00:00:00
abstract::We demonstrate that interspecific sequence conservation can provide a systematic guide to the identification of functional cis-regulatory elements within a large expanse of genomic DNA. The test was carried out on the otx gene of Strongylocentrotus purpuratus. This gene plays a major role in the gene regulatory networ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0618
更新日期:2002-06-01 00:00:00
abstract::The cellular control of the switch from embryonic to fetal globin formation in man was investigated with studies of globin expression in erythroid cells of 35- to 56-day-old embryos. Analyses of globins synthesized in vivo and in cultures of erythroid progenitors (burst-forming units, BFUe) showed that cells of the yo...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90441-6
更新日期:1987-09-01 00:00:00
abstract::The cornea is one of the most densely innervated structures of the body. In the developing chicken embryo, nerves from the ophthalmic trigeminal ganglion (OTG) innervate the cornea in a series of spatially and temporally regulated events. However, little is known concerning the signals that regulate these events. Here...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2010.04.032
更新日期:2010-08-01 00:00:00
abstract::In adult vertebrates, fibroblast growth factor (FGF) synergizes with many hematopoietic cytokines to stimulate the proliferation of hematopoietic progenitors. In vertebrate development, the FGF signaling pathway is important in the formation of some derivatives of ventroposterior mesoderm. However, the function of FGF...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9205
更新日期:1999-04-15 00:00:00
abstract::The contributions that control of cell proliferation and cell growth make to developmental regulation of organ and body size remain poorly explored, particularly with respect to endocycles in polyploid tissues. The epithelium of the marine chordate Oikopleura dioica is composed of a fixed number of cells grouped in te...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0834
更新日期:2002-12-01 00:00:00
abstract::Urodeles begin gastrulation with much of their presumptive mesoderm in the superficial cell layer, all of which must move into the deep layers during development. We studied the morphogenesis of superficial mesoderm in the urodeles Ambystoma maculatum, Ambystoma mexicanum, and Taricha granulosa. In all three species, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0718
更新日期:2002-08-15 00:00:00
abstract::Heparan sulfate proteoglycans are important modulators of growth factor signaling in a variety of patterning processes. Secreted growth factors that play critical roles in angiogenesis bind to heparan sulfate, and this association is affected by 6-O-sulfation of the heparan sulfate chains. Addition of 6-O-sulfate is c...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.05.032
更新日期:2005-08-15 00:00:00
abstract::In many organisms, like Caenorhabditis elegans and Drosophila melanogaster, establishment of spatial patterns and definition of cell fate are driven by the segregation of determinants in response to spatial cues, as early as oogenesis or fertilization. In these organisms, a family of conserved proteins, the PAR protei...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.03.001
更新日期:2005-06-15 00:00:00