Abstract：
:In the present study, we have investigated the role of tyrosine kinase activity during early retinal development in Xenopus laevis. The protein tyrosine kinase (PTK) inhibitors lavendustin A and genistein were used to determine the possible role of tyrosine kinase activity during retinal development in vivo and in vitro. Application of the inhibitors to early embryonic retina disrupted the pattern of lamination in the developing retina. The plexiform layers were severely disorganized or were no longer apparent, and photoreceptor morphogenesis was disrupted. Immunocytochemical analysis verified the presence of focal adhesions in dissociated retinal neuroepithelial cells isolated from St 25 embryos. Application of the PTK inhibitors blocked focal adhesion assembly in these primary cultured cells. To further investigate the regulation of focal adhesions by PTK activity, we examined the effect of lavendustin A on cultured XR1 glial cells. Lavendustin A produced a dose-dependent decrease in the proportion of XR1 cells displaying focal adhesions. Taken together, these results suggest that tyrosine kinase activity is essential for regulating neuroepithelial cell adhesion, migration and morphogenesis during retinal development. Furthermore, the disruption of retinal development may, in part, be due to the inhibition of integrin-mediated signaling.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Li M,Babenko NA,Sakaguchi DSdoi
10.1016/j.ydbio.2003.10.006subject
Has Abstractpub_date
2004-02-01 00:00:00pages
209-21issue
1eissn
0012-1606issn
1095-564Xpii
S0012160603006195journal_volume
266pub_type
杂志文章abstract：:Skeletal muscle fibers express members of the myosin heavy chain (MyHC) gene family in a fiber-type-specific manner. In avian skeletal muscle it is the expression of the slow MyHC isoforms that most clearly distinguishes slow- from fast-contracting fiber types. Two hypotheses have been proposed to explain fiber-type-s...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8619
更新日期：1997-08-01 00:00:00
abstract：:Previous studies from this laboratory have identified a novel alpha-D-mannosidase on the sperm plasma membranes of several species, including man, which may have a role in fertilization. The polyclonal antibody raised against an isoform of the enzyme purified from rat epididymal fluid was found to cross-react with the...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1050
更新日期：1995-02-01 00:00:00
abstract：:Fibronectin (Fn) plays an important part in the branching morphogenesis of salivary gland, lung, and kidney. Here, we examine the effect of the conditional knockout of Fn in the mammary epithelium [Fn(MEp-/-)] on postnatal mammary gland development, using Cre-loxP-mediated gene knockout technology. Our data show that ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.07.001
更新日期：2010-10-01 00:00:00
abstract：:Transcription factors are key orchestrators of the emergence of neuronal diversity within the developing spinal cord. As such, the two paralogous proteins Pax3 and Pax7 regulate the specification of progenitor cells within the intermediate neural tube, by defining a neat segregation between those fated to form motor c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.06.014
更新日期：2017-12-01 00:00:00
abstract：:The sizes of amino acid pools in growing Xenopus laevis oocytes have been measured. The total free amino acid content per oocyte increases nearly 25-fold during oocyte growth. Together, glutamic acid and aspartic acid account for approximately 59-75% of the total amino acid pool in Xenopus oocytes. On the other hand, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(87)90480-5
更新日期：1987-11-01 00:00:00
abstract：:Septins are guanosine triphosphate-binding proteins that are evolutionally conserved in all eukaryotes other than plants. They function as multimeric complexes that interact with membrane lipids, actomyosin, and microtubules. Based on these interactions, septins play essential roles in the morphogenesis and physiologi...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.09.012
更新日期：2016-11-15 00:00:00
abstract：:The correct assembly of junction components, such as E-cadherin and beta-catenin, into the zonula adherens is fundamental for the function of epithelia, both in flies and in vertebrates. In C. elegans, however, the cadherin-catenin system is not essential for general adhesion, raising the question as to the genetic ba...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0113
更新日期：2001-02-01 00:00:00
abstract：:Previous studies suggest that sensory innervation of muscles is patterned by motor innervation. Muscle afferent projections mirror motor projections after various experimental manipulations and muscle afferents fail to project to muscle in the absence of motoneurons. It is not known, however, whether muscle afferents ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8583
更新日期：1997-06-01 00:00:00
abstract：:Thrombospondin type 1 repeat (TSR) superfamily members regulate diverse biological activities ranging from cell motility to inhibition of angiogenesis. In this study, we verified that mouse protein O-fucosyltransferase-2 (POFUT2) specifically adds O-fucose to TSRs. Using two Pofut2 gene-trap lines, we demonstrated tha...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.07.008
更新日期：2010-10-01 00:00:00
abstract：:DNA amplification for two major DNA puffs (II/2B and II/9A) of the fungus fly Sciara coprophila increases steeply from 17 to 19 days after hatching (18 degrees C), resulting in almost 20-fold more DNA at these loci in mature larval salivary glands than in adult tissues. At 19 days after hatching when gene amplificatio...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1287
更新日期：1993-11-01 00:00:00
abstract：:Insulin-producing beta-cells are present as single cells or in small clusters distributed throughout the pancreas of the Xenopus laevis tadpole. During metamorphic climax when the exocrine pancreas dedifferentiates to progenitor cells, the beta-cells undergo two changes. Insulin mRNA is down regulated at the beginning...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.01.038
更新日期：2009-04-15 00:00:00
abstract：:Mesenchymal-epithelial interactions are an important source of information for pattern formation during organogenesis. In the developing excretory system, one of the secreted mesenchymal factors thought to play a critical role in patterning the growth and branching of the epithelial ureteric bud is GDNF. We have teste...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.04.008
更新日期：2005-07-01 00:00:00
abstract：:The polypeptide sequence predicted from the Pem oncofetal gene cDNA contains a homeodomain most closely related to the paired class. Pem, paired class member orthodenticle, and the bicoid maternal gene product homeodomains all have lysine residues at a recognition helix position implicated in DNA-binding specificity. ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90467-h
更新日期：1991-07-01 00:00:00
abstract：:Much of boundary formation during development remains to be understood, despite being a defining feature of many animal taxa. Axial patterning of Hydra, a member of the ancient phylum Cnidaria which diverged prior to the bilaterian radiation, involves a steady-state of production and loss of tissue, and is dependent o...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.11.006
更新日期：2020-02-15 00:00:00
abstract：:Despite 30 years of Hox gene study, we have a remarkably limited knowledge of the downstream target genes that Hox transcription factors regulate to confer regional identity. Here, we have used a microarray approach to identify genes that function downstream of a single vertebrate Hox gene, zebrafish hoxb1a. This gene...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.06.012
更新日期：2007-09-15 00:00:00
abstract：:We have used amphibian gastrulation as a model system to study the action of the extracellular matrix (ECM) glycoprotein tenascin on mesodermal cell migration. Tenascin function was assayed in vitro during spreading of isolated cells from the dorsal marginal zone (DMZ) and during cell migration from DMZ explants. Plas...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90256-i
更新日期：1990-02-01 00:00:00
abstract：:Oral-facial-digital type I (OFDI) syndrome is an X-linked male lethal developmental disorder. It is ascribed to ciliary dysfunction and characterized by malformation of the face, oral cavity, and digits. Conditional inactivation using different Cre lines allowed us to study the role of the Ofd1 transcript in limb deve...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.09.020
更新日期：2011-01-15 00:00:00
abstract：:As the germ band shortens in Drosophila melanogaster embryos, cell shape changes cause segments to narrow anteroposteriorly and to lengthen dorsoventrally. One of the genes required for this retraction process is the hindsight (hnt) gene. hnt encodes a nuclear Zinc-finger protein that is expressed in the extraembryoni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9409
更新日期：1999-10-01 00:00:00
abstract：:Mammalian heart development involves complex morphogenetic events which lead to the formation of fully separated left and right atrial and ventricular chambers from a tubular heart. Separation of left and right ventricular chambers is dependent on a single structure, the interventricular septum (IVS), which has both m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.02.045
更新日期：2006-06-15 00:00:00
abstract：:To feed or breathe, the oral opening must connect with the gut. The foregut and oral tissues converge at the primary mouth, forming the buccopharyngeal membrane (BPM), a bilayer epithelium. Failure to form the opening between gut and mouth has significant ramifications, and many craniofacial disorders have been associ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.09.029
更新日期：2014-12-01 00:00:00
abstract：:We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (blood vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibod...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1999.9246
更新日期：1999-05-01 00:00:00
abstract：:A reexamination of the cDNA clones of the Strongylocentrotus purpuratus sea urchin egg receptor for sperm resulted in several important changes to the sequence. By using both rapid amplification of cDNA ends (RACE) and Northern blot analysis for confirmation, the corrected deduced amino acid sequence was shown to lack...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8504
更新日期：1997-04-01 00:00:00
abstract：:Non-syndromic mitral valve prolapse (MVP) is the most common heart valve disease affecting 2.4% of the population. Recent studies have identified genetic defects in primary cilia as causative to MVP, although the mechanism of their action is currently unknown. Using a series of gene inactivation approaches, we define ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.03.003
更新日期：2020-07-01 00:00:00
abstract：:Cardiomyocyte differentiation overall has been analyzed in vivo and in vitro at the molecular level by homologous recombination, gene mutation studies, and by transgenics; however, the roles of many signal transduction mechanisms that drive this differentiation process are still not fully understood. One set of signal...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(02)00080-5
更新日期：2003-03-15 00:00:00
abstract：:To examine the conditions of cell composition necessary for formation of a complete tadpole, defect embryos of 35 series were prepared by removing particular cells from 16- and 32-cell embryos of Xenopus laevis. These defect embryos were cultured and their development was examined. Formation of a complete tadpole did ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1223
更新日期：1995-08-01 00:00:00
abstract：:Metathoracic limb buds were removed unilaterally from Locusta migratoria embryos at 30% of embryonic development, thereby depriving limb-innervating neurons of the opportunity of innervating their normal target muscles. The operated embryos were allowed to hatch and develop to adulthood, and then the connections betwe...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90335-5
更新日期：1985-02-01 00:00:00
abstract：:In the head of an embryo, a layer of mesenchyme surrounds the brain underneath the surface ectoderm. This cranial mesenchyme gives rise to the meninges, the calvaria (top part of the skull), and the dermis of the scalp. Abnormal development of these structures, especially the meninges and the calvaria, is linked to si...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.07.015
更新日期：2019-11-15 00:00:00
abstract：:The interstitial cells of hydra form a multipotent stem cell system, producing terminally differentiated nerve cells and nematocytes during asexual growth. Under well-fed conditions the interstitial cell population doubles in size every 4 days. We have investigated the possible role of nerve cells in regulating this b...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90089-2
更新日期：1985-08-01 00:00:00
abstract：:LIM-Kinase (LIMK), including LIMK1 and LIMK2, is the only known catalytic protein among LIM-family molecules. It is well known that LIMK phosphorylates and inactivates cofilin, an actin-depolymerizing factor regulating actin reorganization, while in vivo functions have remained to be elucidated. In the present study, ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0512
更新日期：2002-01-15 00:00:00
abstract：:Primitive blood constitutes the ventralmost mesoderm in amphibians, and its cleavage-stage origin reveals important clues about the orientation of the dorsal/ventral axis in the embryo. In recent years, investigators employing various lineage-labeling strategies have reported disparate results for the origin of primit...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2002.0717
更新日期：2002-08-01 00:00:00
