Abstract：
:Mammalian eggs remain arrested at metaphase of the second meiotic division (metII) for an indeterminate time before fertilization. During this period, which can last several hours, the continued attachment of sister chromatids is thought to be achieved by inhibition of the protease separase. Separase is known to be inhibited by binding either securin or Maturation (M-Phase)-Promoting Factor, a heterodimer of CDK1/cyclin B1. However, the relative contribution of securin and CDK/cyclin B1 to sister chromatid attachment during metII arrest has not been assessed. Although there are conditions in which either CDK1/cyclinB1 activity or securin can prevent sister chromatid disjunction, principally by overexpression of non-degradable cyclin B1 or securin, we find here that separase activity is primarily regulated by securin and not CDK1/cyclin B1. Thus the CDK1 inhibitor roscovitine and an antibody we designed to block the interaction of CDK1/cyclin B1 with separase, both failed to induce sister disjunction. In contrast, securin morpholino knockdown specifically induced loss of sister attachment, that could be restored by securin cRNA rescue. During metII arrest separase appears primarily regulated by securin binding, not CDK1/cyclin B1.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Nabti I,Reis A,Levasseur M,Stemmann O,Jones KTdoi
10.1016/j.ydbio.2008.06.036subject
Has Abstractpub_date
2008-09-15 00:00:00pages
379-86issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(08)01045-2journal_volume
321pub_type
杂志文章abstract：:Amphibian embryos from the genus Xenopus are among the best species for understanding early vertebrate development and for studying basic cell biological processes. Xenopus, and in particular the diploid Xenopus tropicalis, is also ideal for functional genomics. Understanding the behavior of genes in this accessible m...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.04.023
更新日期：2004-07-15 00:00:00
abstract：:During limb development, Sonic hedgehog (SHH) and HOX proteins are considered among the most important factors regulating digit number and identity. SHH signaling prevents the processing of GLI3 into a short form that functions as a strong transcriptional repressor. Gli3 mutant limbs are characterized by a severe poly...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.07.023
更新日期：2007-10-15 00:00:00
abstract：:During epithelial development cells become polarized along their apical-basal axis and some epithelia also exhibit polarity in the plane of the tissue. Mutations in the gene encoding a Drosophila Pak family serine/threonine kinase, dPak, disrupt the follicular epithelium that covers developing egg chambers during ooge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.02.034
更新日期：2007-05-15 00:00:00
abstract：:Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.05.006
更新日期：2020-08-01 00:00:00
abstract：:Trophoblast cells are characterized by an invasive behavior into the surrounding uterine tissue. In rodents, an early peri-/endovascular type of invasion exerted by trophoblast giant cells can be distinguished from a late interstitial type carried out by glycogen trophoblast cells. Analysis of the molecular mechanisms...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.040
更新日期：2004-07-15 00:00:00
abstract：:Gap junctions are intercellular channels made of connexins (Cxs) that allow direct communication between adjacent cells. Modulation of Cxs has been associated with abnormal development and function of the mammary gland and breast cancer. However, the mechanisms underlying their expression during normal mammary gland a...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.06.011
更新日期：2016-08-01 00:00:00
abstract：:Symplastic spermatids (sys) male mice are sterile due to a recessive mutation that causes defective adhesion between spermatids and Sertoli cells within the seminiferous epithelium. We show that the mutation in sys mice involves a deletion of 1.24 Mb of chromosome 14. Comparative genomic analysis suggests that this re...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.054
更新日期：2006-10-15 00:00:00
abstract：:Sine Oculis (So), the founding member of the SIX family of homeobox transcription factors, binds to sequence specific DNA elements and regulates transcription of downstream target genes. It does so, in part, through the formation of distinct biochemical complexes with Eyes Absent (Eya) and Groucho (Gro). While these c...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.03.012
更新日期：2014-06-15 00:00:00
abstract：:Chromatin regulators contribute to the developmental control of gene expression. In the nematode Caenorhabditis elegans, the roles of chromatin regulation in development have been explored in several contexts, including vulval differentiation. The synthetic multivulva (synMuv) genes are regulators of vulval developmen...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2014.05.018
更新日期：2014-08-15 00:00:00
abstract：:Adherent cultures of E10.5 rat neuroepithelial cells (NEP cells) from the caudal neural tube require FGF (fibroblast growth factor) and CEE (chick embryo extract) to proliferate and maintain an undifferentiated phenotype in culture. Epidermal growth factor (EGF) does not support E10.5 NEP cells in adherent culture and...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8592
更新日期：1997-06-15 00:00:00
abstract：:We have previously shown that the Caenorhabditis elegans gut-specific esterase gene (Ce-ges-1) has the unusual ability to be expressed in different modules of the embryonic digestive tract (anterior pharynx, posterior pharynx, and rectum) depending on sequence elements within the Ce-ges-1 promoter. In the present pape...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0442
更新日期：2001-11-15 00:00:00
abstract：:We wish to identify genes involved in mediating early lineage decisions in the mouse embryo. F9 teratocarcinoma cells treated with retinoic acid (RA) in suspension culture develop into embryoid bodies (EBs) with an outer layer of visceral endoderm. In order to identify genes that are involved in establishing this extr...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1236
更新日期：1993-09-01 00:00:00
abstract：:Many insects undergo katatrepsis, essential reorganization by the extraembryonic membranes that repositions the embryo. Knockdown of the zen gene by RNA interference (RNAi) prevents katatrepsis in the milkweed bug Oncopeltus fasciatus. However, the precise morphogenetic defect has been uncertain, and katatrepsis itsel...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.06.036
更新日期：2009-09-15 00:00:00
abstract：:Genome integrity in the developing germ line is strictly required for fecundity. In proliferating somatic cells and in germ cells, there are mitotic checkpoint mechanisms that ensure accurate chromosome segregation and euploidy. There is growing evidence of mitotic cell cycle components that are uniquely required in t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.03.011
更新日期：2015-06-15 00:00:00
abstract：:In vertebrates, hematopoietic and vascular progenitors develop from ventral mesoderm. The first primitive wave of hematopoiesis yields embryonic red blood cells, whereas progenitor cells of subsequent definitive waves form all hematopoietic cell lineages. In this report we examine the development of hematopoietic and ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1998.8887
更新日期：1998-05-15 00:00:00
abstract：:Bone development is dependent on the functionality of three essential cell types: chondrocytes, osteoclasts and osteoblasts. If any of these cell types is dysfunctional, a developmental bone phenotype can result. The bone disease osteopetrosis is caused by osteoclast dysfunction or impaired osteoclastogenesis, leading...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.06.018
更新日期：2010-08-15 00:00:00
abstract：:Effective transgenesis methods have been successfully employed in many organisms including zebrafish. However, accurate spatiotemporal control of transgene expression is still difficult to achieve. Here we describe a system for chemical-inducible gene expression and demonstrate its feasibility for generating transgeni...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2008.04.042
更新日期：2008-08-01 00:00:00
abstract：:In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1015
更新日期：1995-01-01 00:00:00
abstract：FOREWORD:The neural crest has been the main object of my investigations during my career in science, up to now. It is a fascinating topic for an embryologist because of its two unique characteristics: its large degree of multipotency and the fact that its development involves a phase during which its component cells mi...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2018.07.019
更新日期：2018-12-01 00:00:00
abstract：:We have investigated the function of the neurogenic gene Notch (N) during development of the adult sensilla of Drosophila. Heat pulses were applied to flies carrying the temperature-sensitive Notch allele Nts1 at different larval and pupal stages. We can show that the reduction of Notch+ function during a short interv...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(90)90147-b
更新日期：1990-11-01 00:00:00
abstract：:Stimulation of epithelial cell cycling by the neuropeptide head activator was analyzed in Hydra magnipapillata and compared with the action of head activator on bud formation and tentacle formation during head regeneration. The results obtained indicate that head activator treatment stimulates epithelial cell division...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8491
更新日期：1997-03-01 00:00:00
abstract：:Retinoic acid (RA) signaling is required for normal development of multiple organs. However, little is known about how RA influences the initial stages of lung development. Here, we used a combination of genetic, pharmacological and explant culture approaches to address this issue, and to investigate how signaling by ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2005.10.045
更新日期：2006-03-01 00:00:00
abstract：:The chick spinal cord can regenerate following injury until advanced developmental stages. It is conceivable that changes in stem/progenitor cell plasticity contribute to the loss of this capacity, which occurs around E13. We investigated the contribution of proliferation, phenotypic changes in radial glia progenitors...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.05.569
更新日期：2009-08-15 00:00:00
abstract：:Integrins are alphabeta transmembrane receptors that function in key cellular processes, including cell adhesion, differentiation, and extracellular matrix deposition through interactions with extracellular, membrane, and cytoplasmic proteins. We previously identified and cloned a muscle beta1 integrin cytoplasmic bin...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/s0012-1606(03)00304-x
更新日期：2003-09-01 00:00:00
abstract：:Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2019.07.014
更新日期：2019-11-15 00:00:00
abstract：:A genetically complex mutant, two-faced (tfd), which causes the production of extra eye and antennal tissue on the dorsal head cuticle, has been analyzed for connectivity to and projection patterns in the central brain of nerves derived from these extra structures. It has been found that the extra antennal nerves freq...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90370-7
更新日期：1985-01-01 00:00:00
abstract：:The location and distribution of neural crest-derived Schwann cells during development of the peripheral nerves of chick forelimbs were examined using chick-quail chimeras. Neural crest cells were labeled by transplantation of the dorsal part of the neural tube from a quail donor to a chick host at levels of the neura...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90014-8
更新日期：1992-03-01 00:00:00
abstract：:Angiogenesis relies on specialized endothelial tip cells to extend toward guidance cues in order to direct growing blood vessels. Although many of the signaling pathways that control this directional endothelial sprouting are well known, the specific cellular mechanisms that mediate this process remain to be fully elu...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.05.011
更新日期：2015-08-15 00:00:00
abstract：:The axonal functions that act in the formation of the neuronal network have been shown to occur in close interdependence with the tissue that surrounds the growing axons. However, little is known about the molecular building blocks underlying axonal functions, although more than 400 axonal proteins have been identifie...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(86)90090-4
更新日期：1986-11-01 00:00:00
abstract：:We have analyzed several aspects of the development of flies carrying mutations at the Glued locus. Optic lobe abnormalities in individuals heterozygous for the original Glued allele were previously shown to result from an action of this mutation in the retinula cells. We have estimated when the functioning of this ge...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(83)90256-7
更新日期：1983-09-01 00:00:00
