Abstract：
:Amphibian embryos from the genus Xenopus are among the best species for understanding early vertebrate development and for studying basic cell biological processes. Xenopus, and in particular the diploid Xenopus tropicalis, is also ideal for functional genomics. Understanding the behavior of genes in this accessible model system will have a significant and beneficial impact on the understanding of similar genes in other vertebrate systems. Here we describe the analysis of 219,270 X. tropicalis expressed sequence tags (ESTs) from four early developmental stages. From these, we have deduced a set of unique expressed sequences comprising approximately 20,000 clusters and 16,000 singletons. Furthermore, we developed a computational method to identify clones that contain the complete coding sequence and describe the creation for the first time of a set of approximately 7000 such clones, the full-length (FL) clone set. The entire EST set is cloned in a eukaryotic expression vector and is flanked by bacteriophage promoters for in vitro transcription, allowing functional experiments to be carried out without further subcloning. We have created a publicly available database containing the FL clone set and related clustering data (http://www.gurdon.cam.ac.uk/informatics/Xenopus.html) and we make the FL clone set publicly available as a resource to accelerate the process of gene discovery and function in this model organism. The creation of the unique set of expressed sequences and the FL clone set pave the way toward a large-scale systematic analysis of gene sequence, gene expression, and gene function in this vertebrate species.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Gilchrist MJ,Zorn AM,Voigt J,Smith JC,Papalopulu N,Amaya Edoi
10.1016/j.ydbio.2004.04.023subject
Has Abstractpub_date
2004-07-15 00:00:00pages
498-516issue
2eissn
0012-1606issn
1095-564Xpii
S0012160604003136journal_volume
271pub_type
杂志文章abstract：:The mesoderm of the sea urchin embryo conventionally is divided into two populations of cells; the primary mesenchyme cells (PMCs), which produce the larval skeleton, and the secondary mesenchyme cells (SMCs), which differentiate into a variety of cell types but do not participate in skeletogenesis. In this study we e...
journal_title：Developmental biology
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journal_title：Developmental biology
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更新日期：1987-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章,评审
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2017-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
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pub_type： 杂志文章
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90256-x
更新日期：1989-12-01 00:00:00
abstract：:In many organisms early embryogenesis is characterised by a period refractory to transcription. In Caenorhabditis elegans, the one-cell embryo is transcriptionally inactive, but at around eight-cell stage transcription is activated in the somatic lineage. This model suggests that histone tail modifications associated ...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.04.010
更新日期：2011-07-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2004.03.023
更新日期：2004-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.9970
更新日期：2001-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1997.8682
更新日期：1997-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(92)90242-9
更新日期：1992-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1991-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0012-1606(89)90304-7
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journal_title：Developmental biology
pub_type： 杂志文章
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journal_title：Developmental biology
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更新日期：1999-12-01 00:00:00
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pub_type： 杂志文章
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更新日期：1987-04-01 00:00:00
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pub_type： 杂志文章
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更新日期：2005-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1984-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2001-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(89)90058-4
更新日期：1989-06-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2009.04.038
更新日期：2009-07-15 00:00:00
abstract：:A small number of signaling pathways are used repeatedly during organogenesis, and they can have drastically different effects on the same population of cells depending on the embryonic stage. How cellular competence changes over developmental time is not well understood. Here we used Xenopus, mouse, and human pluripo...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2017.11.018
更新日期：2018-02-01 00:00:00
abstract：:Chicken talpid(3) mutant embryos have a wide range of Hedgehog-signalling related defects and it is now known that the talpid(3) gene product encodes a novel protein essential for Hedgehog signalling which is required for both activator and repressor functions of Gli transcription factors (Davey, M.G., Paton, I.R., Yi...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
