Abstract:
:We examined the development of epithelial Na+ conductance in 6- and 7-day post coitus (p.c.) preimplantation rabbit embryos using the whole-cell patch-clamp technique on dissociated rabbit trophectodermal cells and by immunocytochemical localization using a polyclonal antibody directed against subunits of an apical epithelial Na+ channel on the intact blastocyst. In Day 6 and 7 p.c. trophectodermal cells, we observed an outwardly rectified whole-cell Na+ current. The current-voltage characteristics did not differ between the 6- and the 7-day p.c. cells. Replacement of Na+ with the impermeant cation N-methyl-D-glucamine in the pipette or bath reduced outward currents and inward currents, respectively, indicating that the current was Na(+)-dependent. Treatment of 7-day p.c. cells with 100 microM amiloride, benzamil, or ethylisopropyl amiloride (EIPA) blocked the whole-cell currents within 5 min. However, the current of the Day 6 p.c. embryo was not blocked by amiloride. The amiloride block at Day 7 p.c. was only partially reversible after 15 min of continuous perfusion of the bath with an amiloride-free solution. The apparent dissociation constant (Ki) for amiloride, benzamil, and EIPA was 12, 50, and 16 microM, respectively, when measured 5 min after drug addition. Immunolocalization studies of blastocysts with a polyclonal antibody raised against a high amiloride affinity Na+ channel isolated from bovine kidney revealed no specific binding to the trophectodermal cells at Day 6 p.c.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Robinson DH,Bubien JK,Smith PR,Benos DJdoi
10.1016/0012-1606(91)90289-fsubject
Has Abstractpub_date
1991-10-01 00:00:00pages
313-21issue
2eissn
0012-1606issn
1095-564Xpii
0012-1606(91)90289-Fjournal_volume
147pub_type
杂志文章abstract::During development of Dictyostelium discoideum, the cellular specific activity of beta-glucosidase increases before aggregation, declines to low levels during pseudoplasmodium formation, and increases rapidly during culmination. In addition, two electrophoretically distinct isozymes of beta-glucosidase are present at ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90407-6
更新日期:1987-10-01 00:00:00
abstract::The scalloped and vestigial genes are both required for the formation of the Drosophila wing, and recent studies have indicated that they can function as a heterodimeric complex to regulate the expression of downstream target genes. We have analyzed the consequences of complete loss of scalloped function, ectopic expr...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9939
更新日期:2000-12-15 00:00:00
abstract::In a previous study (K.-I. Sato et al., 1999, Dev. Biol. 209, 308-320), we presented evidence that a Src-related protein-tyrosine kinase (PTK), named Xyk, may act upstream of the calcium release in fertilization of the Xenopus egg. In the present study, we examined whether PTK activation of phospholipase Cgamma (PLCga...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9782
更新日期:2000-08-15 00:00:00
abstract::In the small-optic-lobes (sol) and sine oculis (so) mutants of Drosophila melanogaster extensive cell death occurs in the optic lobes during the first half of pupal development. Gynandromorph flies show that the sol mutation acts primarily on cells of the medulla cortex. Degeneration of medullar ganglion cells occurs ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90050-2
更新日期:1984-07-01 00:00:00
abstract::LIM-Kinase (LIMK), including LIMK1 and LIMK2, is the only known catalytic protein among LIM-family molecules. It is well known that LIMK phosphorylates and inactivates cofilin, an actin-depolymerizing factor regulating actin reorganization, while in vivo functions have remained to be elucidated. In the present study, ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0512
更新日期:2002-01-15 00:00:00
abstract::Temporally-regulated maternal RNA translation is essential for embryonic development, with defective degradation resulting in stalled 2-cell embryos. We show that DDX1, a DEAD box protein implicated in RNA transport, may be a key regulator of maternal RNA utilization. DDX1 protein localizes exclusively to cytoplasmic ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2019.07.014
更新日期:2019-11-15 00:00:00
abstract::The categorical data set is an important data class in experimental biology and contains data separable into several mutually exclusive categories. Unlike measurement of a continuous variable, categorical data cannot be analyzed with methods such as the Student's t-test. Thus, these data require a different method of ...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2010.08.018
更新日期:2010-12-01 00:00:00
abstract::Mammalian sperm-egg adhesion occurs when sperm bind to the zona pellucida of the egg. In this study with guinea pig gametes, we have asked if sperm can initiate binding to the zona before and after the acrosome reaction and if the sperm surface protein PH-20 is involved in the binding at these two stages. Sperm bindin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90191-6
更新日期:1987-06-01 00:00:00
abstract::Cellular dedifferentiation underlies topical issues in biology such as regeneration and nuclear cloning and has common features in plants and animals. In plants, this process characterizes the transition of differentiated leaf cells to protoplasts (plant cells devoid of cell walls) and is accompanied by global chromat...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.03.023
更新日期:2007-06-15 00:00:00
abstract::Tyrosinase is the rate-limiting enzyme for the production of melanin pigmentation. In the mouse and other animals, homozygous null mutations in the Tyrosinase gene (Tyr) result in the absence of pigmentation, i.e. albinism. Here we used the CRISPR/Cas9 system to generate mono- and bi-allelic null mutations in the Tyr ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2014.06.017
更新日期:2014-09-01 00:00:00
abstract::Xenopus laevis frogs from laboratory stocks normally lay eggs exhibiting extensive size variability. We find that these initial size differences subsequently affect the size of the embryos prior to the onset of growth, and the size of tadpoles during the growth period. Even though these tadpoles differ in size, their ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.03.014
更新日期:2020-06-15 00:00:00
abstract::Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.03.008
更新日期:2005-06-01 00:00:00
abstract::This work has identified the enzymes involved in the activation and conjugation of the ubiquitin-like protein NED-8 in Caenorhabditis elegans. A C. elegans conjugating enzyme, UBC-12, is highly specific in its ability to utilize NED-8 as a substrate. Immunostaining shows that NED-8 is conjugated in vivo to a major tar...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9847
更新日期:2000-10-01 00:00:00
abstract::The task of the visual system is to translate light into neuronal encoded information. This translation of photons into neuronal signals is achieved by photoreceptor neurons (PRs), specialized sensory neurons, located in the eye. Upon perception of light the PRs will send a signal to target neurons, which represent a ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2011.07.006
更新日期:2011-10-01 00:00:00
abstract::The distribution and ontogeny of four neuropeptides in developing chick lumbosacral sensory and sympathetic ganglia were studied using immunohistochemical techniques. Antibodies to two of these peptides, substance P (SP) and calcitonin gene-related peptide (CGRP), stained small neurons in the medial part of the dorsal...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90137-5
更新日期:1986-08-01 00:00:00
abstract::NK genes are related pan-metazoan homeobox genes. In the fruitfly, NK genes are clustered and involved in patterning various mesodermal derivatives during embryogenesis. It was therefore suggested that the NK cluster emerged in evolution as an ancestral mesodermal patterning cluster. To test this hypothesis, we cloned...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.02.013
更新日期:2008-05-15 00:00:00
abstract::We previously demonstrated that male mice deficient in the soluble adenylyl cyclase (sAC) are sterile and produce spermatozoa with deficits in progressive motility and are unable to fertilize zona-intact eggs. Here, analyses of sAC(-/-) spermatozoa provide additional insights into the functions linked to cAMP signalin...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.05.038
更新日期:2006-08-15 00:00:00
abstract::The elongation of embryo and tissue is a key morphogenetic event in embryogenesis and organogenesis. Notochord, a typical chordate organ, undergoes elongation to perform its regulatory roles and to form the structural support in the embryo. Notochord elongation is morphologically similar across all chordates, but asci...
journal_title:Developmental biology
pub_type: 杂志文章,评审
doi:10.1016/j.ydbio.2018.11.009
更新日期:2019-04-15 00:00:00
abstract::The ectoderm of early Xenopus gastrula is competent to become induced to neural tissue, but dorsal ectoderm is more neural competent than ventral ectoderm. It is a tenable, but as yet untested possibility that the higher neural competence of dorsal gastrula ectoderm is dependent on the presence of the dorsal mesoderm....
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(92)90168-g
更新日期:1992-07-01 00:00:00
abstract::The tadpole-type larva of Ciona has emerged as an intriguing model system for the study of neurodevelopment. The Ciona intestinalis connectome has been recently mapped, revealing the smallest central nervous system (CNS) known in any chordate, with only 177 neurons. This minimal CNS is highly reminiscent of larger CNS...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2018.09.023
更新日期:2019-04-15 00:00:00
abstract::We have used a subtractive method to clone novel messages enriched in the heart. Here we show that one such message, bves (blood vessel/epicardial substance) is a novel protein that is highly conserved between chicken and mouse. The bves message is detected at high levels in early chick hearts. Using anti-Bves antibod...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9246
更新日期:1999-05-01 00:00:00
abstract::The transcription factors GATA4, GATA5 and GATA6 are important regulators of heart muscle differentiation (cardiomyogenesis), which function in a partially redundant manner. We identified genes specifically regulated by individual cardiogenic GATA factors in a genome-wide transcriptomics analysis. The genes regulated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2017.11.017
更新日期:2018-02-01 00:00:00
abstract::Trypsin-like activity is secreted from eggs of many species at fertilization, and this activity is believed to be critical for the block to polyspermy. Here we show that a cortical granule serine protease of sea urchins is the major and perhaps only protease family member important for fertilization. Zymography assays...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9299
更新日期:1999-07-01 00:00:00
abstract::Specific binding of spermatozoa to the zona pellucida that surrounds mammalian eggs is a key step in the fertilization process. However, the sperm proteins that recognise zona pellucida receptors remain contentious despite longstanding research efforts to identify them. Here we present evidence that proacrosin, a tiss...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.04.001
更新日期:2007-06-15 00:00:00
abstract::In Hydra long-range interactions between head, bud, and foot formation take place in addition to short-range interactions which are recognized as induction phenomena, occurring when transplants are placed into an environment of disparate positional value. Here the long-range promotion of foot formation is analyzed. Th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1995.1015
更新日期:1995-01-01 00:00:00
abstract::CHARGE syndrome is caused by spontaneous loss-of-function mutations to the ATP-dependant chromatin remodeller chromodomain-helicase-DNA-binding protein 7 (CHD7). It is characterised by a distinct pattern of congenital anomalies, including cardiovascular malformations. Disruption to the neural crest lineage has previou...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2015.06.017
更新日期:2015-09-01 00:00:00
abstract::This work describes unknown aspects of chordotonal organ (ChO) morphogenesis revealed in post-embryonic stages through the use of new fluorescently labeled markers. We show that towards the end of embryogenesis a hitherto unnoticed phase of cell migration commences in which the cap cells of the ventral ChOs elongate a...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2016.01.009
更新日期:2016-03-01 00:00:00
abstract::To examine whether the 200-kDa neurofilament protein (200K NFP) is involved in mechanically stabilizing axons, we studied the developmental appearance of immunoreactivity to nonphosphorylated and phosphorylated 200K NFP at the neuromuscular junction. Polyinnervated rat muscle fibers become singly innervated during the...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(88)90422-8
更新日期:1988-11-01 00:00:00
abstract::GATA4, a transcription factor expressed in the proximal small intestine but not in the distal ileum, maintains proximal-distal distinctions by multiple processes involving gene repression, gene activation, and cell fate determination. Friend of GATA (FOG) is an evolutionarily conserved family of cofactors whose member...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.07.022
更新日期:2008-10-01 00:00:00
abstract::Type II collagen is a major component of hyaline cartilage, and has been suggested to be causally involved in promoting chondrogenesis during embryonic development. In the present study we have performed an immunohistochemical analysis of the distribution of type II collagen during several early stages of embryonic ch...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/s0012-1606(89)80026-0
更新日期:1989-02-01 00:00:00