Abstract:
:Intracellular pH (pHi) measurements were performed in surf clam (Spisula solidissima) oocytes before and after artificial activation or fertilization [evidenced by germinal vesicle breakdown (GVBD)] by the dimethyloxazolidinedione (DMO) and 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) methods. Results using both methods showed increases of pHi of 0.3 pH unit after activation by excess K+. Using BCECF, we found an increase of similar magnitude after fertilization or after the addition of serotonin. By contrast, GVBD did not occur when the pHi was increased to similar or even higher levels by exposing the oocytes to ammonia. In sodium-free seawater, excess K+ induced GVBD but the pHi of K+-activated oocytes decreased significantly below the resting level of unactivated oocytes. The pHi increases in K+-activated oocytes were otherwise proportional to the external Na+ concentration. The amiloride derivatives dimethylamiloride and hexamethylene amiloride (at 10-50 microM) efficiently inhibited the K+-induced increase of pHi but did not block GVBD. These two derivatives were able, however, to retard K+-induced GVBD, hexamethylene amiloride being the more efficient. This retardation of K+-induced GVBD could be abolished by the simultaneous addition of ammonia. Taken altogether, these results show that a pHi increase, driven by a typical Na+/H+ exchanger, follows activation of surf clam oocytes but that this pHi increase is neither sufficient nor required for GVBD, though it does allow its progression at an optimal rate.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Dubé F,Eckberg WRdoi
10.1006/dbio.1997.8682subject
Has Abstractpub_date
1997-10-01 00:00:00pages
41-54issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(97)98682-6journal_volume
190pub_type
杂志文章abstract::The origin and development of mouse kidney vasculature were examined in chorioallantoic grafts of early kidney rudiments and of experimentally induced explants of separated metanephric mesenchymes. Whole kidney rudiments developed into advanced stages, expressed the segment-specific antigenic markers of tubules and th...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90180-x
更新日期:1983-04-01 00:00:00
abstract::Division of a female Drosophila stem cell produces a daughter stem cell and a cystoblast. The cystoblast produces a syncytial cluster of 16 cells by precisely four mitotic divisions and incomplete cytokinesis. Mutations in genes required for cystoblast differentiation, such as bag-of-marbles, block syncytial cluster f...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9346
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abstract::During its development the epidermis of the pf/pf mutant mouse is invaded by cells from the underlying dermis. These invading cells establish a network of cells including fibroblasts, endothelial cells, and nerve fibers, throughout the epidermis. Subsequent to these events the keratohyalin protein, filaggrin, is drast...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(84)90194-5
更新日期:1984-04-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1999.9285
更新日期:1999-06-15 00:00:00
abstract::The source of neurite outgrowth in explant cultures of normal adult Apteronotus spinal cord was examined. Explants which contained the central region of spinal cord, including ependyma, showed neurite outgrowth in culture. Explants which did not contain ependyma showed no neurite outgrowth. It is concluded that the ep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(87)90062-5
更新日期:1987-02-01 00:00:00
abstract::Proper calcium channel and insulin signaling are essential for normal brain development. Leaner mice with a mutation in the P/Q-type voltage-gated calcium channel, Cacna1a, develop cerebellar atrophy and mutations in the homologous human gene are associated with increased migraine and seizure tendency. Similarly, abno...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00
abstract::We examined the structure, expression, and possible functions of a nanos homologue during gametogenesis and blastogenesis in the colonial ascidian Botryllus primigenus. An approximately 1.3-kb-long cDNA was cloned; it was termed BpNos since the deduced amino acid sequence (288 aa) contained 2 Nanos-like CCHC zinc fing...
journal_title:Developmental biology
pub_type: 杂志文章
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90243-9
更新日期:1983-12-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2004.05.034
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(85)90358-6
更新日期:1985-05-01 00:00:00
abstract::Development of the craniofacial structures requires the precise differentiation of cranial neural crest cells into osteoblasts or chondrocytes. Here, we explore the epigenetic and non-epigenetic mechanisms that are required for the development of craniofacial chondrocytes. We previously demonstrated that the acetyltra...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2020.05.006
更新日期:2020-08-01 00:00:00
abstract::Optimal maturation of oocytes and successful development of preimplantation embryos is essential for reproduction. We performed DNA microarray analyses of ovarian transcripts and identified glial cell line-derived neurotrophic factor (GDNF) secreted by cumulus, granulosa, and theca cells as an ovarian factor stimulate...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2007.12.029
更新日期:2008-03-01 00:00:00
abstract::A new method is presented for preparation of highly purified plasma membranes from fertilized sea urchin eggs. The purified plasma membranes are in vesicle form and are highly enriched in ouabain inhibitable, Na+/K+ ATPase activity. Analysis of membrane proteins by sodium dodecyl sulfate-gel electrophoresis indicates ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(83)90106-9
更新日期:1983-06-01 00:00:00
abstract::During endochondral bone formation, vascular invasion initiates the replacement of avascular cartilage by bone. We demonstrate herein that the cartilage-specific overexpression of VEGF-A(164) in mice results in the hypervascularization of soft connective tissues away from cartilage. Unexpectedly, perichondrial tissue ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.05.552
更新日期:2009-08-15 00:00:00
abstract::Suppression of wing fate and specification of haltere fate in Drosophila by the homeotic gene Ultrabithorax is a classical example of Hox regulation of serial homology (Lewis, E.B. 1978. Nature 276, 565-570) and has served as a paradigm for understanding homeotic gene function. We have used DNA microarray analyses to ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.12.022
更新日期:2006-03-15 00:00:00
abstract::Bone morphogenetic protein-4 (BMP-4) induces epidermis and represses neural fate in Xenopus ectoderm. Our previous findings implicate p42 Erk MAP kinase (MAPK) in the response to neural induction. We have examined the effects of BMP-4 on MAPK activity in gastrula ectoderm. Expression of a dominant negative BMP-4 recep...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2001.0338
更新日期:2001-08-15 00:00:00
abstract::The regulation of early embryonic development in the amphibian Xenopus laevis depends largely upon translational and post-translational regulatory mechanisms to direct the complex cytodifferentiations that take place during early cleavage and blastula formation. The cell cycle dependence of protein synthesis was exami...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(91)90460-k
更新日期:1991-07-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2000.9981
更新日期:2001-01-15 00:00:00
abstract::Rapidly growing human teratocarcinoma cells (Tera-2) can be induced to differentiate into quiescent, nontumorigenic cells expressing neuronal markers. To more closely mimic the in vivo conditions for tumor growth, we grew Tera-2 cells in three-dimensional collagen gel cultures. The undifferentiated cells proliferated ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1994.1016
更新日期:1994-01-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.2002.0839
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pub_type: 杂志文章
doi:10.1016/j.ydbio.2009.09.026
更新日期:2009-12-15 00:00:00
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journal_title:Developmental biology
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abstract::This investigation provides the first systematic determination of the cellular and molecular progression of vocal fold (VF) epithelium development in a murine model. We define five principal developmental events that constitute the progression from VF initiation in the embryonic anterior foregut tube to fully differen...
journal_title:Developmental biology
pub_type: 杂志文章
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更新日期:2015-03-15 00:00:00
abstract::The vertebrate pituitary gland is a key endocrine control organ that contains six distinct hormone secreting cell types. In this study, we analyzed the role of direct cell-to-cell Delta-Notch signaling in zebrafish anterior pituitary cell type specification. We demonstrate that initial formation of the anterior pituit...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.04.019
更新日期:2008-07-15 00:00:00
abstract::Octopamine is an important neuroactive substance that modulates several physiological functions and behaviors of invertebrate species. Its biosynthesis involves two steps, one of which is catalyzed by Tyramine beta-hydroxylase enzyme (TBH). The Tbetah gene has been previously cloned from Drosophila melanogaster, and n...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2003.07.019
更新日期:2003-12-01 00:00:00
abstract::To investigate the molecular regulation of embryonic somite formation and development, we have cloned the full-length cDNA and characterized the embryonic expression profile of chicken Paraxis, a member of a novel family of basic helix-loop-helix (bHLH) proteins, which has been suggested to play a role in paraxial mes...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1006/dbio.1997.8663
更新日期:1997-09-01 00:00:00
abstract::We have previously shown that the adenosine analog 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR), an activator of AMP-activated protein kinase (AMPK), stimulates an increase in AMPK activity and induces meiotic resumption in mouse oocytes [Downs, S.M., Hudson, E.R., Hardie, D.G., 2002. A potential rol...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2005.11.039
更新日期:2006-03-15 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2008.11.031
更新日期:2009-03-01 00:00:00
abstract::The fate of bacteriophage lambda-DNA was examined after injection into the fertilized eggs of Xenopus laevis. Injection of a large amount of lambda-DNA (ca. 24 ng) into a fertilized Xenopus egg induced the formation around the injected DNA of a giant nucleus-like structure which was surrounded by an apparently normal ...
journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/0012-1606(86)90155-7
更新日期:1986-08-01 00:00:00
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journal_title:Developmental biology
pub_type: 杂志文章
doi:10.1016/j.ydbio.2006.10.012
更新日期:2006-12-15 00:00:00