Abstract：
:In the mouse embryo, the onset of zygotic transcription occurs at the end of the first cell cycle, upon completion of DNA replication. We show that the nonhistone chromosomal protein HMG-I, whose translocation into the pronuclei of one-cell embryos is linked to this first round of DNA synthesis, plays a critical role in the activation of zygotic transcription. Indeed, microinjection of purified HMG-I results in a higher nuclear accumulation of the protein and triggers an earlier activation of zygotic transcription, an effect which is abolished by the preincubation of the protein with a specific antibody directed against its AT-hook DNA-binding motifs. Significantly, microinjection of this antibody also prevents the normal onset of transcription in the embryo, suggesting that endogenous HMG-I is similarly involved in this process. Finally, microinjection of the exogenous protein modifies chromatin structure as measured by in situ accessibility to DNase I. We propose that general chromosomal architectural factors such as HMG-I can modulate the accessibility of chromatin to specialized regulatory factors, thereby promoting a transcriptionally competent state.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Beaujean N,Bouniol-Baly C,Monod C,Kissa K,Jullien D,Aulner N,Amirand C,Debey P,Käs Edoi
10.1006/dbio.2000.9668subject
Has Abstractpub_date
2000-05-15 00:00:00pages
337-54issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)99668-4journal_volume
221pub_type
杂志文章abstract：:In warm-blooded vertebrates it is generally accepted that after early stages of development new neurons are not added to the retina. Contrary to this belief, we show here that hatched chickens have a zone of proliferating cells at the peripheral margin of the retina, similar to that of fish and amphibians. We found th...
journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：2000-04-15 00:00:00
abstract：:Using gene-specific probes derived from four mouse gamma-crystallin cDNAs, we have examined the regulation of different members of the mouse gamma-crystallin gene family during lens development. Our analysis revealed that, while the different gamma-crystallin genes appear to be coordinately activated during embryogene...
journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章,评审
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journal_title：Developmental biology
pub_type： 杂志文章
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