Abstract:
:A series of chimeras was generated by injecting single normal, parthenogenetic, or androgenetic blastomeres carrying transgenic markers under the zona pellucida of nontransgenic eight-cell embryos. These chimeras were cultured to the blastocyst stage and sectioned, and the transgenic component was detected by in situ hybridization. No statistically significant difference was found among the normal, parthenogenetic, and androgenetic chimeras in the number of chimeric blastocysts with a transgenic contribution to the inner cell mass (ICM), the trophectoderm, or both the ICM and trophectoderm. Since androgenetic and parthenogenetic cells were present in chimeras at a high frequency in both the ICM and trophectoderm at the blastocyst stage, but not in similar chimeras at late gastrulation, these cells must not respond normally to developmental signals subsequent to blastocyst formation and prior to late gastrulation.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Thomson JA,Solter Ddoi
10.1016/s0012-1606(89)80028-4subject
Has Abstractpub_date
1989-02-01 00:00:00pages
580-3issue
2eissn
0012-1606issn
1095-564Xpii
S0012-1606(89)80028-4journal_volume
131pub_type
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