Abstract：
:During digit development, the correct balance of chondrogenic signals ensures the recruitment of undifferentiated cells into the cartilage lineage or the maintenance of cells at the undifferentiated stage. WNT/β catenin maintains the pool of progenitor cells, whereas TGFβ signalling promotes cartilage differentiation by inducing Sox9 expression. Moreover, WNT5A promotes the degradation of β catenin during mouse limb development. Although these mechanisms are well established, it is still unknown whether the signalling pathway downstream WNT5A is also involved in early chondrogenesis during digit formation. Thus, the aim of this study was to determine the role of WNT5A during the recruitment of progenitor cells during digit development. Our results showed that WNT5A activated calcium (Ca2+) release in the undifferentiated region during digit development. Further, the blockade of Ca2+ release or calcineurin (CaN) or nuclear factor of activated T-cells (NFAT) functions resulted in an inhibition of cartilage differentiation. Together, our results demonstrate that non canonical WNT5A-Ca2+-CaN-NFAT signalling plays a key role during embryonic digit development in vivo promoting the competence for chondrogenic signals and also acts as a permissive factor for chondrogenesis independently of cell death mechanisms.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Farrera-Hernández A,Marín-Llera JC,Chimal-Monroy Jdoi
10.1016/j.ydbio.2020.10.003subject
Has Abstractpub_date
2021-01-01 00:00:00pages
86-95eissn
0012-1606issn
1095-564Xpii
S0012-1606(20)30274-8journal_volume
469pub_type
杂志文章abstract：:The dopaminergic neurons of the basal ganglia play critical roles in CNS function and human disease, but specification of dopamine neuron phenotype is poorly understood in vertebrates. We performed an in vivo screen in zebrafish to identify dopaminergic neuron enhancers, in order to facilitate studies on the specifica...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2011.01.023
更新日期：2011-04-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2012.06.028
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journal_title：Developmental biology
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journal_title：Developmental biology
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doi：10.1016/s0012-1606(03)00255-0
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journal_title：Developmental biology
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journal_title：Developmental biology
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2010.08.029
更新日期：2010-11-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0016
更新日期：1996-01-10 00:00:00
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更新日期：1983-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1006/dbio.2001.0397
更新日期：2001-10-01 00:00:00
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journal_title：Developmental biology
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doi：10.1016/0012-1606(84)90036-8
更新日期：1984-07-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2016.02.007
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(91)90089-l
更新日期：1991-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2001.0416
更新日期：2001-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(88)90199-6
更新日期：1988-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2015.08.004
更新日期：2015-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.2000.0127
更新日期：2001-03-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1995.1050
更新日期：1995-02-01 00:00:00
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journal_title：Developmental biology
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pub_type： 杂志文章
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更新日期：2000-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 历史文章,杂志文章
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更新日期：2011-10-15 00:00:00
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pub_type： 杂志文章
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更新日期：2006-07-15 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2006.06.005
更新日期：2006-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章,评审
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更新日期：2019-01-15 00:00:00
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pub_type： 杂志文章
doi：10.1006/dbio.2000.9671
更新日期：2000-05-01 00:00:00
