Abstract:
:Soluble N-ethylmalameide-sensitive factor attachment protein receptor (SNARE) proteins are present in mammalian sperm and could be involved in critical membrane fusion events during fertilization, namely the acrosome reaction. Vesicle-associated membrane protein/synaptobrevin, a SNARE on the membrane of a vesicular carrier, and syntaxin 1, a SNARE on the target membrane, as well as the calcium sensor synaptotagmin I, are present in the acrosome of mammalian sperm (human, rhesus monkey, bull, hamster, mouse). Sperm SNAREs are sloughed off during the acrosome reaction, paralleling the release of sperm membrane vesicles and acrosomal contents, and SNARE antibodies inhibit both the acrosome reaction and fertilization, without inhibiting sperm-egg binding. In addition, sperm SNAREs may be responsible, together with other sperm components, for the asynchronous male DNA decondensation that occurs following intracytoplasmic sperm injection, an assisted reproduction technique that bypasses normal sperm-egg surface interactions. The results suggest the participation of sperm SNAREs during membrane fusion events at fertilization in mammals.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Ramalho-Santos J,Moreno RD,Sutovsky P,Chan AW,Hewitson L,Wessel GM,Simerly CR,Schatten Gdoi
10.1006/dbio.2000.9745subject
Has Abstractpub_date
2000-07-01 00:00:00pages
54-69issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(00)99745-8journal_volume
223pub_type
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