Abstract：
:Recombinant limb buds constructed of an ectodermal jacket containing dissociated anterior wing bud mesoderm cells form limb-like structures lacking anteroposterior polarity. Polarity can be restored by implanting a small piece of polarizing region into the recombinant limb. We used recombinant limbs to study expression of Msx1 and Msx2 and 5' Hoxd genes in limb development. Detectable mesodermal expression of Msx1 and Msx2 was lost during preparation of the recombinants. When expression was restored both genes were expressed in a symmetrical fashion in the apical mesoderm whether or not the recombinant was polarized. The normal anterior pattern of Msx1 and Msx2 was never reestablished. The roles Msx1 and Msx2 play in limb patterning are at present not clear. In nonpolarized recombinant limbs the 5' Hoxd genes were sequentially and uniformly expressed in the subridge mesoderm across the anteroposterior axis. They exhibited nested domains of expression showing exclusively distal restriction. In polarized recombinants the 5' Hoxd genes were expressed progressively in an asymmetric, nested fashion adjacent to the implanted polarizing region; there was also a distal mesodermal region further from the implant that did not express the most 5' Hoxd genes. Both of these observations are similar to the normal limb. Our results demonstrate that the polarizing region is not necessary for the activation or maintenance of 5' Hoxd gene expression but that its presence is required in order for the normal pattern to be established. We propose that the expression of the Hoxd genes is an intrinsic property of rapidly growing limb cells and that their expression is related to the limb bud proximodistal outgrowth. Our data also support the influence of the polarizing region upon Hoxd gene expression in two ways. First there is a short-range action responsible for the normal asymmetric expression and second a long-range effect suppressing the expression of 5'-Hoxd genes by the anterior cells.
journal_name
Dev Bioljournal_title
Developmental biologyauthors
Ros MA,Lyons GE,Mackem S,Fallon JFdoi
10.1006/dbio.1994.1296subject
Has Abstractpub_date
1994-11-01 00:00:00pages
59-72issue
1eissn
0012-1606issn
1095-564Xpii
S0012-1606(84)71296-6journal_volume
166pub_type
杂志文章abstract：:The development of the mammalian neocortex requires radial and tangential migration of cells. Radial migration of differentiated neurons from the ventricular zone (VZ) is well established. It is hypothesised that an earlier phase of tangential migration of mitotically active cells lays down a widespread periodically s...
journal_title：Developmental biology
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abstract：:We have performed a detailed analysis of the expression pattern of the three gnathostome Otx classes in order to gain new insights into their functional evolution. Expression patterns were examined in the developing eye of a chondrichthyan, the dogfish, and an amniote, the chick, and compared with the capacity of para...
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journal_title：Developmental biology
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journal_title：Developmental biology
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更新日期：1984-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2016-11-15 00:00:00
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journal_title：Developmental biology
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journal_title：Developmental biology
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更新日期：2011-04-01 00:00:00
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journal_title：Developmental biology
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doi：10.1016/0012-1606(85)90437-3
更新日期：1985-09-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1998-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0174
更新日期：1996-08-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1004
更新日期：1994-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(85)90318-5
更新日期：1985-02-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2020.10.003
更新日期：2021-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/0012-1606(84)90192-1
更新日期：1984-04-01 00:00:00
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更新日期：2004-04-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0257
更新日期：1996-10-10 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1994.1284
更新日期：1994-10-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1993.1123
更新日期：1993-05-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.0265
更新日期：1996-11-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
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更新日期：1992-01-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1006/dbio.1996.8472
更新日期：1997-02-01 00:00:00
abstract：:The chicken retina is capable of limited regeneration. In response to injury, some Müller glia proliferate and de-differentiate into progenitor cells. However, most of these progenitors fail to differentiate into neurons. The Notch pathway is upregulated during retinal regeneration in both fish and amphibians. Since t...
journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2007.09.046
更新日期：2007-12-01 00:00:00
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journal_title：Developmental biology
pub_type： 杂志文章
doi：10.1016/j.ydbio.2018.07.006
更新日期：2018-10-01 00:00:00
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更新日期：2012-11-01 00:00:00
abstract：:Rho GTPases control signal transduction pathways that link cell surface receptors to a variety of intracellular responses. They are best known as regulators of the actin cytoskeleton, but in addition they control cell polarity, gene expression, microtubule dynamics and vesicular trafficking. Through these diverse func...
journal_title：Developmental biology
pub_type： 杂志文章,评审
doi：10.1016/j.ydbio.2003.06.003
更新日期：2004-01-01 00:00:00
